scholarly journals Intestinal Parasitosis

1970 ◽  
Vol 31 (3) ◽  
pp. 13-16
Author(s):  
Bikash Shakya ◽  
D Bhargava ◽  
S. Shrestha ◽  
B. P. Rijal

Introduction: Intestinal parasitosis is highly prevalent among general population and hospital patients in Nepal. The study aimed to assess the prevalence of intestinal parasitosis among patients in a hospital of Nepal. Methods: A retrospective study was done by analyzing the findings of stool examined from April 2006 to March 2008 at Department of Microbiology of the hospital. χ2-test was used for analytical assessment. Results: Out of 2221 fecal samples, 20.7% were positive, the positivity being 23.9% and 15.8%, respectively in first and second year (P<0.05). Overall infection rate was nearly equal in male and female (20.2% vs. 21.2%). The infection rate was highest among patients of >60 years (25.3%) followed by those of <15 years (21.2%) and 15-59 years (20.1%) ( P>0.05). Ascaris lumbricoides (31.8%) and Entamoeba histolytica (38.0%) were the commonest helminth and protozoa, respectively. Other parasites were Hookworm (18.3%), Hymenolepis nana (2.6%), Taenia solium (2.6%), Strongyloides stercoralis (1.3%) and Giardia lamblia (5.5%). Conclusions: Though, the prevalence of intestinal parasitosis among hospital visiting patients is declining, high detection of A. lumbricoides and E. histolytica signifies the need of public awareness regarding latrine use, water source protection from fecal contamination, proper sanitation and hygienic behavior. Keywords: Ascaris lumbricoides, Entamoeba histolytica, intestinal parasites. DOI: 10.3126/joim.v31i3.2974 Journal of Institute of Medicine, December, 2009; 31(3) 13-16

2020 ◽  
Vol 30 (1) ◽  
pp. 34764
Author(s):  
Andressa Barros Ibiapina ◽  
Janaína Soares Leal ◽  
Pedro Ricardo Alves de Santana ◽  
Marcelo Ribeiro Mesquita ◽  
Tito Lívio da Cunha Lopes ◽  
...  

AIMS: This research aims to determine the epidemiology and the spatial distribution of intestinal parasitosis in the city of Teresina.METHODS: A cross-sectional study was carried out based on the data of parasitological fecal exams performed in the Laboratory Raul Bacelar between January, 2014 and July, 2017. In addition to the prevalence of intestinal parasitosis and polyparasitism, we verified the association of these diseases with gender, zone and period of the year by means of the chi-squared test, whereas the relation with age was analyzed by the Mann-Kendall tests and multiple comparisons of age classes. The spatial distribution was performed using the QGIS georeferencing software.RESULTS: The prevalence of enteroparasitosis in Teresina is 17,8% with Ascaris lumbricoides being the most common species, due to the precarious sanitary conditions of the city. The prevalence of individuals with polyparasitism is 3,13%, in which an association between the species Entamoeba coli and Entamoeba histolytica/dispar was found. There was no relation between intestinal parasitosis with gender, but we verified that individuals in rural areas are more susceptible to these diseases. The species Ascaris lumbricoides and Entamoeba histolytica/dispar occur more frequently in the first and second semester, respectively. We observed that there is an apparent tendency to increase cases of E. histolytica/dispar and reduction of cases of Giardia sp. according to aging. Mapping intestinal parasitosis showed us that there is a prevalence between one and 20% in most of Teresina's neighborhoods, and Ascariasis embodies at least 40% of cases of enteroparasitosis in these neighborhoods.CONCLUSIONS: Investments in basic sanitation and new epidemiological investigations must be carried out to control intestinal parasitosis in Teresina, emphasizing that children and the elderly should be considered priority groups in these programs.


1968 ◽  
Vol 2 (4) ◽  
pp. 169-214 ◽  
Author(s):  
Ruy Gomes de Moraes

1 - Foram examinadas as fezes de 2.666 indivíduos, operários e funcionários de duas Emprésas industriais, situadas, uma na cidade do Rio de Janeiro e outra no Estado do Rio (Brasil); 2 - Dos 2.666 indivíduos, 1941 (72.80%) estavam parasitados por um ou mais helmintos e 725 (27,20%) tinham seus exames de fezes negativos; 3 - De cada um dos 2.666 indivíduos foi feito um hemograma completo, tendo sido aproveitada a taxa de eosinófilos que, em associação com os exames de fezes, constituiu o objeto principal dêste trabalho. 4 - Na Tabela A observa-se o número de vêzes em que cada um dos vermes foi observado e seus respectivos percentuais. Embora não seja um trabalho de epidemiologia, verifica-se que 46,81% são infestados pelo Trichuris trichiura; 23,85% pelo Strongyloides stercoralis; 22,46% pelo Necator americanus e/ou Ancylostoma duodenale; 20,51% pelo Ascaris lumbricóides; 1,65% pelo Schistosoma mansoni; 0.67% pelo Enterobius vermicularis; 0,26% por Taenia solium ou T. saginata e 0,11% por Hymenolepis nana; 5 - Os exames de fezes foram feitos pelos métodos de Faust (ou de Ritchie), de Willis, de Baermann e de sedimentação; 6 - A eosinofilia anotada foi a relativa ou em seu percentual, sendo considerada hipereosinofilia uma taxa de eosinófilos igual ou superior a 5% (Eo > 5%); 7 - Foram abordados de modo conciso os fatores que provocam oscilações na eosinofilia normal tais como a idade, a raça, as horas do nictêmero, os fatores físicos, o sexo, os fatores químicos e outros; 8 - Tratou-se de modo mais extenso das diferenças entre as hipereosinofilias parasitárias e não parasitárias, tendo sido focalizada a dinâmica da eosinofilia traduzida na curva de Lavier. 9 - A distribuição dos 2.666 casos foi feita pelos diferentes graus de eosinofilia, tendo sido levantados gráficos e traçadas curvas sôbre a distribuição de cada helminto e de suas associações. 10 - Por ser necessário à explanação do assunto, foi criado o "índice eosinofilico", o qual corresponde à relação entre o número de casos de um determinado grupo com Eo > 5% e Eo < 5%. Para o total de casos positivos, ao "índice eosinofílico" denominamos "índice eosinofílico médio" em para o total dos negativos "índice eosinofílico residual"; 11 - Estabelecendo-se o "índice eosinofílico", pode-se ajuizar a capacidade eosinofilogênica de cada helminto isoladamente, bem como a de suas associações; 12 - Atenção especial foi dada aos problemas da existência da hipereosinofilia nos casos com exames coprológicos negativos para helmintos, tendo-se passado em revista vários dos aspectos biológicos que o assunto comporta; 13 - Outra questão de grande importância clínica explanada neste trabalho é a do encontro de casos de parasitismo por vermes, sem hipereosinofilia. O autor, baseado em seus dados e em outros colhidos na literatura sôbre o assunto, discute a fisiopatologia da eosinopoiese nas helmintoses e ojerece uma interpretação para êste fato ainda não defintivamente esclarecido.


Author(s):  
Arcelia González-García ◽  
Claudia Hernández-Salas ◽  
Rosa Maria Martínez-Ortiz ◽  
Lilia González-Martínez

Introduction: Nowadays in our state, intestinal parasitic diseases are still endemic, with a greater frequency in rural and mountainous urban areas. After the years have elapsed since the last national survey, it was necessary to carry out a new survey to know the prevalence rates of intestinal parasitosis and to compare the results obtained between both studies. Such knowledge would be of great value in developing health strategies and designing intestinal parasitic infection control programs. Objective: to determine the current prevalence rates of intestinal parasites, to compare the results with those of the survey obtained. Methods: a random cross-sectional descriptive study was carried out during the months of December 2018 to March 2019 in a random sample of both sexes from the population of urban communities and the province of Zacatecas, Zac. Each one had a stool sample collected that was analyzed by the direct examination method, the Willis concentration technique, and the Kato-Katz examination; A questionnaire was also applied to them. Results: when comparing the results of the national and state surveys of intestinal parasitic infections carried out in 2009 and 2018, it was found that, in general, infections by parasites decreased, both helminths and pathogenic protozoa, although those infected with commensals increased their frequency in that of 2019. There was also a decrease in the frequency of infections by all species of soil-transmitted helminths, Trichuris trichiura, Ascaris lumbricoides, ancylostomideos, and Strongyloides stercoralis, as well as by the pathogenic protozoa Giardia lamblia and Entamoeba histolitica. Conclusions: when comparing the results of national and state surveys of intestinal parasite infections carried out in 2018 and 2019, it was found that in general the frequencies of infections with intestinal parasites decreased. The finding in both studies of a higher frequency of infection with parasites or commensals in the group aged 5 to 14 years (school age), supports the recommendation to place emphasis on control programs for intestinal parasites in this age group.


2018 ◽  
Vol 16 (2) ◽  
pp. 44-46
Author(s):  
Maya Rai ◽  
Ganesh Prasad Neupane ◽  
Dabal Bahadur Dhami ◽  
Thakur Prasad Paudel

Introduction: The parasites can cause different gastrointestinal disorders which have great impact on life quality. Hearing and speech impaired children are unable to maintain proper sanitation, making them more prone to intestinal parasitic infection. Aim and Objective: To know the prevalence of various parasitic infestations among hearing and speech impaired Children. Materials and Methods: Stool specimen were collected from 104 hearing and speech impaired children who were living in private rehabilitation school of chisapani village of Banke district Nepal for a period of 5 month from December 2017 to April 2018 and direct wet mount was prepared using normal saline (0.9%) and Lugol's iodine (0.5%). The wet mount was observed under microscope for parasites. Results: Among 104 individuals intestinal parasites were seen in 25.96 % of students. Giardia lamblia (37.04%) was the most common parasite followed by Ascaris lumbricoides (18.52%) and Entamoeba histolytica (14.82%). Conclusion: The study shows that although the speech and hearing impaired children are more prone to parasitic infection, the prevalence is same as normal people. Giardia lamblia, Ascaris lumbricoides and Entamoeba histolytica were the common parasites. Prompt diagnosis and treatment of infected children should be undertaken.


2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Sandeep Thapa

ObjectiveTo find out the prevalence of Entamoeba species in rural community of Nepal.The purpose of the study is to evaluate Nested PCR, microscopic examination and Elisa assay for detection and differentiation of Entamoeba species.IntroductionNepal being a developing country has many health problems, which escalate in intensity at different times of the year or in epidemic form.Amebiasis is one of the infectious diseases that is highly seen in rural area of Nepal caused by Entamoeba species ((1, 2). Recent reports show that open defecation, drinking untreated water, unsanitary habits and lack of basic health knowledge cause higher mortality and morbidity in our country.E. histolytica is an anaerobic pathogenic parasitic. However, E. dispar and E.moshkovskii exits as non-pathogenic. Likewise, E. histolytica, E. dispar and E. moshkovskii are morphologically identical but genetically distinct species (3).MethodsA total of 270 faecal sample were collected from south eastern terai region of Nepal after the informed consent form. The samples were processed by direct wet smear and formalin ethyl acetate concentration technique (4). Eventually, microscopic examination were performed for the detection of Entamoeba species along with other intestinal parasites. Furthermore, enzyme immunoassay were executed to detect antigens of E. histolytica through ELISA. Additionally, microscopically positive samples for Entamoeba species cysts were further characterized using a Nested- PCR targeting 16S-like ribosomal RNA gene (5). The PCR generate amplicons which was subjected to 2% agarose gels electrophoresis and visualized under UV transilluminator.Results8.52% of the total collected samples were microscopically positive for Entamoeba cysts either singly or in combination with other intestinal parasites. Likewise, among 270 stool sample, viral diarrheal was most significant form of diarrhoea found in 76.67% of patients. Among different organisms, As. Lumbricoids and E. histolytica, G. lambia and H. nana were identified in most of the patients accounting for 11.11%, 8.52%, 2.59% and 1.11% respectively. However, Lumbricoids, G. lambia, Tenia solium and E. histolytica were present in an individual patient while two patient was found with both As. Lumbricoids and G. lambia. Among several symptoms, diarrhoea seems to be the common symptoms infecting all of the patients which is followed by fever and vomiting which accounts for 55.1 % and 46.2% correspondingly. Whereas, nausea appears to be the least common symptoms infecting only 14.4% of patients.Subsequently, 56 cases were PCR positive, 51 cases were ELISA positive whereas 47 were found to be positive by microscopy.ConclusionsMolecular techniques are indeed promising tools for epidemiological studies, particularly in discriminating the pathogenic from the non-pathogenic species of the Entamoeba species. This study reports a new nested multiplex PCR strategy for detection and differentiation of E. histolytica, E. dispar and E. moshkovskii which is highly rapid, specific and sensitive which is useful for proper diagnosis, immunological assay and drug testing.References1. Chaudhary M, Maharjan M. ( 2014). Association of Anaemia with Parasitic Infection in Pregnant Women Attending Antenatal Clinic at Koshi Zonal Hospital. Neplalese Journal of Zoology, 2(1):1-7.2. Tandukar S, Ansari S, Adhikari N, Shrestha A, Gautam J, Sharma B, et al (2013). Intestinal parasitosis in school children of Lalitpur district of Nepal. BMC research notes, 6(1):449.3. Clark C, Espinosa Cantellano M, Bhattacharya A. Entamoeba histolytica: an overview of the biology of the organism (2013). Amebiasis: World Scientific, 1-45.4. Dhanabal J, Selvadoss PP, Muthuswamy K(2014). Comparative study of the prevalence of intestinal parasites in low socioeconomic areas from South Chennai, India. Journal of parasitology research.5. Van den Bossche, D., Cnops, L., Verschueren, J., & Van Esbroeck, M. (2015). Comparison of four rapid diagnostic tests, ELISA, microscopy and PCR for the detection of Giardia lamblia, Cryptosporidium spp. and Entamoeba histolytica in feces. Journal of microbiological methods, 110, 78-84.


Author(s):  
Firas Fahad Habeeb ◽  
Ali Mohammed Abed

The current study was conducted to detect the infection rate of the Entamoeba histolytica and Giardia lamblia parasite and to determine the relationship between them and Helicobacter pylori bacteria for patients attending Shirqat General Hospital in Salah al-Din Governorate during the period from October 2020 to March 2021. The number of examined samples reached 409, as they were examined by direct smear and Concentration methods using an optical microscope. The results of the study showed that the total infection rate was 46 (11.24%) positive samples and 363 negative samples with a rate of (88.7%), the Entamoeba histolytica was dominant with rate 67.38%, followed by Giardia lamblia with rate 32.61%, and the infection rates for males were higher than for females. Where the infection rate of males reached 14.29% (34 out of 238) and 7.02% of females (12 out of 171), respectively. The infection rates varied between age groups, as the highest rate of infection was in the age group ≥ 10-1 years, which amounted to 32.6%, and the lowest rate of infection was in the age group 60-51 years, with a rate of 2.17%. The highest infection rate was in October, which amounted to 14.89%, while the lowest infection rate was in December, with a rate of 7.35%. All positive samples were examined for microscopic examination by rapid antigen test to detect H.pylori bacteria involved in infection with Entamoeba histolytica or Giardia lamblia, and the results showed that 13 (28.26) samples were positive and 33 (71.73%) were negative samples, and the Co-infection infection rate was between the Entamoeba histolytica and H.pylori 9 (29.03%) were higher than that of Giardia lamblia and H.pylori 4 (26.66%).The results of the statistical analysis showed that there were significant differences between the sex of patients infected with these parasites and the rates of infection during the months of the study and the age groups of infected patients.


2019 ◽  
Vol 47 (1) ◽  
pp. 129-136
Author(s):  
Md Aminul Islam Bhuiyan ◽  
Zannatun Nahar Jhinu ◽  
Jaliluzzaman ◽  
Mandira Mukutmoni

A total of 200 stool samples were examined and five species of intestinal parasites were identified. Two of them were protozoans (Entamoeba histolytica and Giardia intestinalis) and the rest of which three species were nematode parasites (Ascaris lumbricoides, Trichuris trichiura and Strongyloids stercoralis). The overall prevalence of parasitic infection was 50%. Individually the prevalence of Ascaris lumbricoides, Entamoeba histolytica, Giardia intestinalis, Trichuris trichiura and Strongyloids stercoralis was 25%, 13%, 6%, 3.5% and 2.5%, respectively. Patients of different age and sex had a variable degree of infection. Children aged under ten years showed the highest prevalence (73.97%) compared to other groups. Accordingly, male patients were found slightly more susceptible to the infection (50.38%) compared to the female (49.29%). People belonging to different occupation and educational qualification had various degree of infection. As expected patients who had no education and poor monthly income, showed the highest prevalence of infection, 77.77% and 90%, respectively. Personal hygiene practices of the patients had much influence on the parasitic prevalence and rate of infection. Respectively 58.82%, 91.42%, 80% and 63.55% prevalence were found among the patients who used to drink unsafe water, wash hands without soap, stay barefooted most of the time and do not trim their nails regularly. The present study supposed to reveal that socioeconomic conditions and behavioral factors may influence the prevalence of parasitic infections. Bangladesh J. Zool. 47(1): 129-136, 2019


2015 ◽  
Vol 16 (6) ◽  
pp. 859-870 ◽  
Author(s):  
Antonio Neres Norberg ◽  
Fabiano Guerra-Sanches ◽  
Paulo R. Blanco Moreira-Norberg ◽  
José Tadeu Madeira-Oliveira ◽  
Aluízio Antonio Santa-Helena ◽  
...  

<p><strong>Objetivo </strong>Considerando que más de la mitad de la población mundial está infectada por protozoarios y helmintos intestinales, con alta prevalencia en las zonas más pobres, esta investigación tuvo como objetivo identificar la prevalencia de los parasitismos entre indígenas de la etnia Terena, establecidos en el Estado de Mato Grosso do Sul.</p><p><strong>Metodología </strong>Se examinaron 134 muestras de heces de individuos de la comunidad indígena. Estas se conservaron en solución de Merthiolate-iodo-formol (MIF). Los exámenes de laboratorio fueron realizados por las técnicas de Hoffman, Pons y Janer; Willis y Kinyoun.</p><p><strong>Resultados </strong>Se identificaron infecciones por helmintos nematodos de las especies <em>Ascaris lumbricoides,</em> Ancylostomidae,<em> Enterobius vermicularis, Strongyloides stercoralis, </em>y<em> Trichuris trichiura</em>; cestodos de la especie <em>Hymenolepis nana, </em>y <em>Taenia</em>spp<em>.</em> También por protozoarios de las especies <em>Cryptosporidium </em>spp.<em>, Giardia lamblia, Endolimax nana, Entamoeba coli, </em>y<em> Entamoeba histolytica</em>. De las muestras investigadas, 23,1% fueron negativas; de los 76,9 % parasitados hubo diferencia estadísticamente no significativa para el parasitismo en hombres y mujeres examinados, de unoa 33 años de edad, y sobre parasitismo mono específico, o con simultaneidad de especies. Como diversidad parasitaria fueron encontradas siete especies de helmintos nematodos y cestodos, y cinco de protozoarios Archamoebae, flagelados y enterozoários. </p><p><strong>Conclusiones</strong> Los resultados fueron la base para la orientación e intervención adecuada, revelando la necesidad de la implantación de medidas gubernamentales y socioeducativas para mejorar las condiciones de vida de esta comunidad.</p>


2014 ◽  
Vol 39 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Hamida Khanum ◽  
Farzana Rahman ◽  
Rimi Farhana Zaman

The present study was conducted to find out the incidence of the intestinal parasites and their prevalence among the teachers, students and staffs of University of Dhaka, Bangladesh. The study revealed that the prevalence of intestinal protozoa and helminth parasites are common among the outdoor patients of University of Dhaka. A total of 350 stool samples was examined in the Pathology department of Medical Center of University of Dhaka from June 2009 to May 2010, four species of intestinal parasites were identified of which two species were protozoa (Entamoeba histolytica and Giardia intestinalis) and two species were nematodes (Ascaris lumbricoides and Trichuris trichiura). The overall prevalence of infestation was 23.14% where Entamoeba histolytica, Giardia intestinalis, Ascaris lumbricoides and Trichuris trichiura were found as 4.86%, 3.71%, 11.14% and 3.43% respectively. Highest prevalence was recorded in Ascaris lumbricoides (11.14%) and the seasonal pattern showed that highest (30%) prevalence occurred in rainy season and lowest (17.19%) in winter season. The prevalence of intestinal parasites was higher in female (30.56%) than in male (22.29%). DOI: http://dx.doi.org/10.3329/jasbs.v39i2.17863 J. Asiat. Soc. Bangladesh, Sci. 39(2): 239-246, December 2013


2013 ◽  
Vol 3 (1) ◽  
pp. 75-77
Author(s):  
Shiv Narayan Yadav

The present studyy examined association between ABO human blood group and helminth parasites. In total 3000 people were investigated and 1300 individuals were found infected with helminth parasites, namely Ascaris lumbricoides (52.46%) Ancylostoma duodenale (45.00%), Trichuris trichura (1.90%), Enterobius vermicularis (0.03%), Taenia solium (0.015%), Hymenolepis nana (0.15%).


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