scholarly journals Nasal Carriage of Staphylococcus aureus among Healthy School Children in Kathmandu Valley

1970 ◽  
Vol 9 ◽  
pp. 139-142 ◽  
Author(s):  
Dev Raj Joshi ◽  
Suraj Narayan Shrestha ◽  
Rajdeep Bomjan ◽  
Kamal Poudel
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
School Children ◽  
Antibiotic Susceptibility ◽  
Clinical Laboratory ◽  
Nasal Carriage ◽  
Diffusion Method ◽  
P Value ◽  
Kathmandu Valley ◽  
Microbiological Methods

Staphylococcus aureus remains one of the most frequently isolated pathogens in both community and hospital practices. Methicillin resistant S. aureus (MRSA) continues to be a major cause of serious infections in the community worldwide. This study was undertaken to determine the prevalence of S. aureus and MRSA in school children in Kathmandu valley and, also to evaluate the antibiotic susceptibility pattern of the isolates. Identification of the S. aureus was confirmed by standard microbiological methods and the antibiotic susceptibility testing was performed by disk diffusion method according to the Clinical Laboratory Standards Institution (CLSI). Of the 324 school children who participated in the study, 38 (11.8%) had S. aureus isolated from nasal swabs. The potential risk factors for the S. aureus carriage showed the antibiotic usage within past 4 weeks to be associated with the rate of the nasal carriage (p value 0.000). Out of 38, two (5.2%) isolates were MRSA. One of the MRSA strains was also resistant to Cloxacillin. Erythromycin and Cloxacillin resistance was present in 3 (7.9%) isolates. Resistance to two or more antibiotics was noted in 2 (5.3%) isolates. However, no strains were resistant to Vancomycin. The results of this study indicated that the carriage of MRSA exists among young healthy school children who lack traditional risk factors for MRSA. Key words: community acquired MRSA; Staphylococcus aureus; nasal carriage; school children DOI: 10.3126/njst.v9i0.3177 Nepal Journal of Science and Technology 9 (2008) 139-142

scholarly journals Detection of Methicillin Susceptible and Resistant Staphylococcus aureus Nasal Carriage and Its Antibiotic Sensitivity among Basic and Clinical Years Medical Students

Healthcare ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 161 ◽  
Author(s):  
Hamed Alzoubi ◽  
Mohammad Al Madadha ◽  
Asma’a Al-Mnayyis ◽  
Muayad Azzam ◽  
Amira Aldawoud ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Medical Students ◽  
Antibiotic Susceptibility ◽  
Healthcare Workers ◽  
Antibiotic Sensitivity ◽  
Nasal Carriage ◽  
Diffusion Method ◽  
P Value ◽  
Susceptibility Data ◽  
Significant Difference

Background: Healthcare workers (HCWs) and medical students can be asymptomatic carriers in transmitting methicillin resistant and susceptible Staphylococcus aureus (MRSA and MSSA). Studying epidemiological and antibiotic susceptibility data is necessary to limit the spread of infections, help with treatment and understand the transmission dynamics of MSSA and MRSA. Our study assessed the rate of MSSA and MRSA nasal carriage and its antibiogram among medical students in basic and clinical years at the University of Jordan. Methods: A total of 210 nasal swabs were randomly collected from participants. MSSA and MRSA were identified by culture, biochemical and other phenotypical analysis methods. Antibiotic susceptibility was determined by the disc diffusion method. Results: The nasal carriage of MSSA was 6.6% and 11.4% and that of MRSA was 1.9% and 2.8% among basic and clinical years, respectively. There was no significant difference for the nasal carriage of MSSA and MRSA among basic and clinical year students (p value ≥ 0.05). MSSA resistance ranged between 25% and 33% for trimethoprim-sulfamethoxazole, tetracycline and ciprofloxacin. For MRSA, the highest resistance was to trimethoprim-sulfamethoxazole and tetracycline (67% to 100%), followed by gentamicin and ciprofloxacin (33% to 67%), in all participants in the study. Conclusion: The difference in the carriage rates of MSSA and MRSA among basic and clinical students was statistically insignificant. The continuous awareness and implementation of infection control procedures and guided patient contact are recommended. The results might also suggest that healthcare workers could be victims in the cycle of MRSA nasal carriage, a theory that needs further study.

Staphylococcus aureus nasal colonization among Vietnamese adults: prevalence, risk factors and antibiotic susceptibility profile

MedPharmRes ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 21-31
Author(s):  
Nguyen Phan ◽  
Hien Pham ◽  
Thuc Nguyen ◽  
Hoai Nguyen
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Nasal Carriage ◽  
Resistance Rate ◽  
High Rate ◽  
Weight History ◽  
Potential Risk Factors ◽  
History Of ◽  
Vietnamese Community

Staphylococcus aureus (S. aureus) has long been recognized as an important human pathogen causing many severe diseases. It is also a part of human normal flora with its ecological niche in the human anterior nares. This study focused on screening S. aureus nasal carriage in community and its relationship to human physiological and pathological factors which have not been studied in Vietnam previously. Two hundred and five volunteers in Ho Chi Minh City from 18 to 35 and over 59 years old both male and female participated in the study. Result showed that the prevalence of S. aureus nasal carriage in southern Vietnamese community was relatively low, only 11.2% (23/205), much lower than that in other international reports on human S. aureus. In addition, nasal carriage of the older age group (> 59 years old, 13.7%) was higher than that of younger age (18-35 years old, 10.4%). Other potential risk factors such as gender, career, height, weight, history of antibiotic usage, daily nasal wash, use of nasal medication sprays, acne problems, smoking and nasal problems showed no significant impact on S. aureus carriage. The obtained S. aureus nasal isolates were all sensitive to vancomycin. Lincomycin and tetracycline had low resistance rate with 4.3 % and 17.4 %, respectively. However, the isolates showed particularly high rate of multidrug resistance (54.2%) In summary, our data provided researchers an overview on S. aureus nasal carriage and antibiotic susceptibility profile of the community- isolated S. aureus in Vietnam. This would serve as valuable information on assessing risk of community-acquired S. aureus infections.

scholarly journals Staphylococcus aureus nasal carriage in the community: a survey from central Italy

2002 ◽  
Vol 129 (2) ◽  
pp. 417-420 ◽  
Author(s):  
G. ZANELLI ◽  
A. SANSONI ◽  
A. ZANCHI ◽  
S. CRESTI ◽  
S. POLLINI ◽  
...  
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Central Italy ◽  
Rare Event ◽  
Nasal Carriage ◽  
High Rate ◽  
Methicillin Resistant ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Patterns

Recently, concern has increased regarding the spread of methicillin-resistant Staphylococcus aureus (MRSA) in the community. We studied 812 subjects from central Italy to establish the rates of nasal carriage of S. aureus, and antibiotic susceptibility patterns, in the community. The prevalence of S. aureus nasal carriage was 30.5%. Only one subject, with predisposing risk factors for acquisition, was identified as carrier of MRSA (prevalence of 0.12%). The presence of MRSA in the community of our area still appears to be a rare event. Among methicillin-susceptible S. aureus (MSSA) isolates, a surprisingly high rate (18%) of resistance to rifampin was observed.

Nasal Carriage of Staphylococcus aureus in Healthy Adults and in School Children

2013 ◽  
Vol 30 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Marina Dinić ◽  
Slavica Vuković ◽  
Dobrila Stanković Đorđević ◽  
Milena Bogdanović
Keyword(s):  
Staphylococcus Aureus ◽  
School Children ◽  
Local Community ◽  
Nasal Carriage ◽  
Diffusion Method ◽  
Healthy Individuals ◽  
Disk Diffusion Method ◽  
Nasal Swabs ◽  
Mueller Hinton Agar ◽  
Therapeutic Problem

Summary Staphylococcus aureus (S. aureus) is a microorganism that colonizes the skin and mucosal surfaces of healthy individuals, but it is also one of the most common causes of community-acquired and hospital infections. Nasal carriage of S. aureus represents a major risk factor for the development of infection with this bacterium. A special therapeutic problem are methicillin-resistant isolates of S. aureus (MRSA). The aim of this study was to assess the nasal carriage of S. aureus in healthy individuals in the local community, and the sensitivity of the microorganism to antibiotics. The study enrolled 56.868 healthy individuals aged 19 to 65 years, and 2.040 healthy school children aged 15 to 19 years. Specimens to be studied were obtained from anterior nares. We used the disk diffusion method (Kirby-Bauer) on Mueller- Hinton agar to assess the sensitivity of isolated S. aureus. S. aureus was isolated in 1.381 (2.34%) respondents. Positive findings were obtained in 2.33% of adult examinees, and in 2.59% of studied school children. We found a low level of susceptibility only to penicillin (5.36%). The susceptibility of S. aureus isolates to all other tested antibiotics was present in a high percentage, with the lowest percentage of susceptibility to doxycycline (71.54%) and erythromycin (86.09%). The highest percentage of susceptibility of tested isolates was reported for fusidic acid (99.27%). In relation to the total number of S. aureus isolates from nasal swabs in adults, MRSA was present in 8.96% (119 isolates), while there were 4 MRSA isolates from nasal swabs in school children. In this study, we established a low percentage of nasal carriage of S. aureus in the population of healthy individuals, but a high percentage of MRSA.

scholarly journals Nasal carriage of Staphylococcus aureus among indigenous people of Toba Qom ethnic, Benjamín Aceval, Paraguay

2018 ◽  
Vol 5 (7) ◽  
pp. 2720
Author(s):  
Paulo Roberto Blanco Moreira Norberg ◽  
Maria Laura Meister Villalba ◽  
Larissa Duarte Ortellado ◽  
Marcelo Coronel ◽  
Paulo César Ribeiro ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
San Francisco ◽  
Clinical Laboratory ◽  
Nasal Carriage ◽  
Multidrug Resistant ◽  
National Committee ◽  
Isolation And Identification ◽  
Indigenous Ethnic Group ◽  
Microbiological Methods ◽  
Invasive Infections

Background: Staphylococcus aureus are Gram positive cocci components of the normal microbiota of the skin and mucosa, but can attack other sites of the organism depending on favourable conditions. The condition of host of this bacterium colonizing the nasal mucosa is recognized as a potential risk factor for subsequent invasive infections. This research had the objective to investigate the prevalence of nasal carriers of S. aureus among Toba Qom indigenous ethnic group of the San Francisco de Asis community, Paraguay, and to determine the sensitivity profile to antimicrobial agents.Methods: 141 samples of the nasal cavities were analyzed. Isolation and identification were performed by conventional microbiological methods, by the staining characteristics of the Gram method, catalase and coagulase tests, mannitol fermentation test and deoxyribonuclease test. Antimicrobial susceptibility test was performed by the agar diffusion technique according to a procedure standardized by the National Committee for Clinical Laboratory Standards.Results: 22 of the 141 individual examined samples were colonized by Staphylococcus aureus, which corresponds to a prevalence of 15.6%.Conclusions: Multidrug-resistant strains were not isolated. All samples presented good sensitivity to most antibiotics except for nalidixic acid, which all samples presented resistance. 

scholarly journals Bacteriological Profile and Antibiotic Susceptibility Pattern of Isolates of Wound Infection In Children Visiting Kanti Children Hospital

2020 ◽  
Vol 7 ◽  
pp. 123-132
Author(s):  
Gita Ghimire ◽  
Chaudhary RP ◽  
Binod Lekhak
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Infection ◽  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Susceptibility Pattern ◽  
Disc Diffusion Method ◽  
Antibiotic Susceptibility Pattern ◽  
Microbiological Methods ◽  
The Status

Objectives: The objectives of this study was to isolate and identify the bacterial etiological agent of wound infection and explore the status of methicillin-resistant Staphylococcus aureus ( MRSA), multidrug Resistant (MDR) and extended spectrum β-lactamase (ESBL) producers’ strains in clinical specimens and to find the antibiotic susceptibility pattern. Methods: A prospective cross sectional study design was conducted from period of February 2014 to October 2014 at Kanti Children Hospital, Kathmandu. The organisms were isolated and identified from pus sample by standard microbiological methods. Antimicrobial susceptibility test was performed by modified the Kirby Bauer disc diffusion method to evaluate the status of MRSA and MDR. ESBL detection was performed by the combined disc diffusion method. Results: Out of 365 specimens collected between the age group below < 2 to 15 years, 210 (57.73%) samples from male patients and 155 (42.47%) from female patients. In the total samples processed, Gram-positive organisms were found to be more prevalent in which Staphylococcus aureus accounts for 135(47.20%), followed by P. aeruginosa 62 (21.67%), E. coli 29 (10.20%), K. pneumoniae 27 (9.44%), Acinetobacter spp. 20 (6.70%), P. vulgaris 7 (2.44%) and CoNS 6 (2.10%). Among the S. aureus isolates, 29 (21.48%) were found MRSA. Of the total Gram-negative organisms isolated, 74 (51.03%) were MDR and 14 (100%) ESBL producer, (P<0.01). S. aureus was found to be the most important and leading cause of wound infection in this study. Conclusion: Thus, routine antibiotic susceptibility testing is recommended for empirical drug therapy and proper management of disease.  

scholarly journals Nasal carriage of Staphylococcus aureus by medical students: Assessment of antibiotic susceptibility and risk factors

2020 ◽  
Author(s):  
Fikriye MİLLETLİ SEZGİN ◽  
Sevgi SARIHAN ◽  
Havva Nur TÜRKOĞLU ◽  
Melihcan YAĞMUR ◽  
Gülgüşa BUCAK ◽  
...  
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Medical Students ◽  
Antibiotic Susceptibility ◽  
Nasal Carriage
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