scholarly journals Ameliorative Effects of the Aqueous Extract of Allium porrum (Wild Leek) against Cisplatin-Induced Nephrotoxicity in Rabbits

2021 ◽  
Vol 30 (2) ◽  
pp. 177-184
Author(s):  
HS Mohammed ◽  
MHS Ahmida ◽  
MF Madi ◽  
A A Abdel-Gayoum
Keyword(s):  
Single Dose ◽  
Positive Control ◽  
Negative Control ◽  
Allium Porrum ◽  
Distilled Water ◽  
Group Iii ◽  
Cisplatin Nephrotoxicity ◽  
Group I ◽  
Daily Dose ◽  
New Zealand Rabbits

The aim of the present study was to investigate the nephroprotective, hypolipidemic and hypoglycemic effects of Allium porrum (leek) in rabbits with cisplatin nephrotoxicity. Forty adult male New Zealand rabbits were divided randomly into four groups (ten rabbits in each group) as follows: Group I: (negative control) (C) received oral daily dose of distilled water for 15 successive days. Groups II: (Leek) (L) received oral daily dose of aqueous leek extract (500mg/kg/day) for 15 successive days. Group III: (positive control) [cisplatin (CP)] received oral daily dose of distilled water for 15 successive days, and subsequently administered single dose of Cisplatin (3.5mg/kg/day) by intraperitoneal injection from day 10 for five days. Groups IV: (Leek and Cisplatin

scholarly journals Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

2020 ◽  
Vol 8 (A) ◽  
pp. 423-427
Author(s):  
Prihantono Prihantono ◽  
Salman Ardi Syamsu ◽  
Nilam Smaradhania ◽  
Mardiana Ahmad ◽  
Nurul Aini Siagian ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Mean Value ◽  
Negative Control ◽  
Interleukin 1Β ◽  
Sprague Dawley ◽  
Leaf Extracts ◽  
Group Iii ◽  
Group I ◽  
The Mean

BACKGROUND: Mastitis is an inflammation of the breast tissue, usually caused by bacteria. Mastitis stimulates pro-inflammatory cytokines. The cytokine interleukin-1β (IL-1β) is a crucial mediator of the inflammatory response. This cytokine has adverse effects of hosting immunity that mediates resistance to pathogens and also exacerbates damage during chronic disease and acute tissue injury. Scaevola taccada (Gaertn.) Roxb. has been used as an ethnomedicine for healing sores in several provinces in Indonesia. AIM: This study aimed to assess the profile of pro-inflammatory cytokine IL-1β through the treatment effect of leaf extracts of S. taccada (Gaertn.) Roxb. as adjuvant for healing mastitis. METHODS: This study was a true control group experiment using the pre-test-post-test control design aimed to measure the effect of hydroalcoholic compounds in leaf extracts of S. taccada on the systemic pro-inflammatory activity of interleukin-1β (IL-1β). The treated animals were 18 mice of Sprague Dawley strain induced by Staphylococcus aureus. These treated mice were divided into three groups in which each group consisted of six mice. The mice in the Group I (negative control) were given 1 ml aquabides/250 g body weight, those in the Group II (positive control) were delivered with 9.6 mg/ml amoxicillin/250 g body weight, and those in the Group III (experimental) were given 9.6 mg amoxicillin/250 g body weight + 400 mg/ml leaf extracts of S. taccada/g body weight for 5 days, respectively. Pathological examinations were carried out from the inflamed tissues to prove the healing process of the treated mice. IL-1Β levels were determined using the enzyme-linked immunosorbent assay method. Data were analyzed using ANOVA and post hoc tests. RESULTS: There were statistically significant differences of IL-1β levels after the administration of leaf extracts of S. taccada among all the treated mice groups at p < 0.05. The Group III had the lowest IL-1β level with the mean value ± 1.45 pg/ml compared to the IL-1β level in the Group II (positive control) with the mean value ± 3.82 pg/ml and the IL-1β level in the Group I (negative control) with the with mean value ± 5.22 pg/ml. The pathological analysis of breast tissues of the treated mice proved that leaf extracts of S. taccada (Gaertn Roxb.) could reduce damaged tissues, cellular infiltration, and subcutaneous edema induced by this pathogenic microorganism. CONCLUSION: Leaf extracts of S. taccada had a significant function as adjuvant for healing mastitis by reducing pro-inflammatory cytokine IL-1β.

scholarly journals PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

2017 ◽  
Vol 13 (3) ◽  
Author(s):  
Condro Suro Miyarso ◽  
Tri Cahyani Widiastuti ◽  
Naelaz Zukhruf Wakhidatul Kiromah
Keyword(s):  
Body Weight ◽  
Testosterone Level ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Group Iii ◽  
Eurycoma Longifolia ◽  
Group I ◽  
Eurycoma Longifolia Jack

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight.  Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

scholarly journals Comparison of Clinical Effectiveness of Deslorelin Acetate and Osaterone Acetate in Dogs with Benign Prostatic Hyperplasia

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1936 ◽  
Author(s):  
Wojciech Niżański ◽  
Małgorzata Ochota ◽  
Christelle Fontaine ◽  
Joanna Pasikowska
Keyword(s):  
Clinical Signs ◽  
Clinical Effectiveness ◽  
Positive Control ◽  
Negative Control ◽  
Intact Male ◽  
Prostate Hyperplasia ◽  
Group Iii ◽  
Group I ◽  
Clinical Action ◽  
Healthy Dogs

This article presents the results of a randomized clinical trial, designed to compare the efficacy and therapeutic profiles of YpozaneTM (osaterone acetate—OA) or SuprelorinTM (deslorelin acetate—DA) in male dogs with clinical signs of benign prostate hyperplasia (BPH). Forty-five intact male dogs were used in the study. The Group I (negative control) included 10 healthy dogs, the Group II (positive control) included 10 dogs with confirmed BPH and no treatment, whereas Group III and IV consisted of dogs with BPH and treated either with DA (15 dogs) or OA (10 dogs). The clinical response, testosterone and estradiol levels, hematology, biochemistry, and adverse effects incidence were evaluated. Both OA and DA proved to be effective for BPH treatment in dogs, as they allowed for the clinical remission in all treated dogs. The complete alleviation of BPH symptoms was noticed sooner with the use of OA (in 80% of dogs from day 7) compared to DA (in 40% of dogs within the first 21 days). The recurrence of clinical signs related to BPH was observed from week 24 in dogs treated with OA, whereas no relapse was noticed in dogs treated with DA at the end of the 36 weeks of the observation period. In 5 dogs (33%) treated with DA, a flare-up effect (increase in the clinical signs associated with BPH) was noticed on day 7. Despite individual differences in the clinical action, both medications were effective and safe options for the treatment of symptoms related to BPH in dogs.

scholarly journals Protective and curative effects of Beta vulgaris on dimethyl 2,2-dichlorovinyl phosphate-exposed albino rats

2019 ◽  
Vol 2 ◽  
pp. 26
Author(s):  
Eucharia O. Nwaichi ◽  
Eka B. Essien ◽  
Uzoamaka Chinonso Ibe
Keyword(s):  
Beta Vulgaris ◽  
Histopathological Examination ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Biochemical Assays ◽  
Before And After ◽  
Liver And Kidney

Background: This study evaluated the effect of Beta vulgaris (beetroot) smoothie on some biochemical parameters on dimethyl 2,2-dichlorovinyl phosphate (DDVP, known as dichlorvos)-exposed albino Wistar rats. Methods: A total of 30 rats of both sexes were grouped into five groups of six animals each. Group I served as the negative control and were not exposed to dichlorvos. Group II served as the positive control and were exposed to dichlorvos but received no smoothie. Group III received 500 mg/kg body weight beetroot smoothie and was not exposed to dichlorvos. Groups IV and V were exposed to dichlorvos but received beetroot before and after exposure, respectively. At the end of the 6-week experiment, the animals were euthanized, the blood samples collected for some biochemical assays while the organs (kidney and liver) were harvested and subjected to histopathological examination. Results: From the biochemical assay, it was observed that the beetroot smoothies regulated and significantly reduced the elevated levels of AST, ALT, urea and creatinine observed in the animals that were exposed to dichlorvos. Additionally, the beetroot was able to regenerate the liver and kidney organs that were damaged on exposure to dichlorvos. Conclusion: This study concluded that beetroot smoothie possesses hepato-protective, hepato-curative as well as nephro-curative properties.

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

Effects of Fluoride on the Mouse Sperm Morphology Test

1987 ◽  
Vol 66 (9) ◽  
pp. 1509-1511 ◽  
Author(s):  
Y. Li ◽  
A.J. Dunipace ◽  
G.K. Stookey
Keyword(s):  
Sperm Morphology ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Genotoxic Effects ◽  
Mouse Sperm ◽  
Abnormal Sperm ◽  
Group I ◽  
Cervical Dislocation

There has been little information and much confusion regarding the genotoxic effects of fluoride. The purpose of this study was to examine the spermatogenic influence of sodium fluoride (NaF) on the germ cells by means of the mouse sperm morphology test. Male mice of genotype B6C3F1 were obtained at about eight weeks of age and maintained on a low-fluoride diet (<0.2 ppm F) and distilled water ad libitum throughout the experiment. At approximately 13 weeks of age, the animals were randomly assigned to eight groups. Group I was intubated with the Maximum Tolerable Dosage (MTD) of NaF (70 mg/kg). Groups II through VI received NaF by stomach intubation at doses of 35, 20, 10, 1, and 0.1 mg/kg, respectively. Group VII served as a negative control and was intubated with distilled water. The positive control, Group VIII, was exposed to a known mutagen, cyclophosphamide (20 mglkg, i.p). The animals were treated daily for five days, and killed by cervical dislocation 35 days after the first exposure to chemicals. Slides of sperm from the cauda epididymides were prepared and blindly scored for morphological abnormalities. Weight of the testes was recorded, and the femurs were saved for fluoride (F) analysis. Analysis of bone F demonstrated the effective absorption of fluoride following intubation. The counts of abnormal sperm and the weights of the testes for mice exposed to NaF doses up to the MTD were not significantly different from those of the negative control. The results of this study showed that NaF did not have adverse effects on mouse sperm morphology.

scholarly journals Protective and curative effects of Beta vulgaris on pesticide dimethyl 2,2-dichlorovinyl phosphate-exposed albino rats

2019 ◽  
Vol 2 ◽  
pp. 26
Author(s):  
Eucharia O. Nwaichi ◽  
Eka B. Essien ◽  
Uzoamaka Chinonso Ibe
Keyword(s):  
Beta Vulgaris ◽  
Histopathological Examination ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Biochemical Assays ◽  
Before And After ◽  
Liver And Kidney

Background: This study evaluated the effect of Beta vulgaris (beetroot) smoothie on some biochemical parameters on dimethyl 2,2-dichlorovinyl phosphate (DDVP, known as dichlorvos)-exposed albino Wistar rats. Methods: A total of 30 rats of both sexes were grouped into five groups of six animals each. Group I served as the negative control and were not exposed to dichlorvos. Group II served as the positive control and were exposed to dichlorvos but received no smoothie. Group III received 500 mg/kg body weight beetroot smoothie and was not exposed to dichlorvos. Groups IV and V were exposed to dichlorvos but received beetroot before and after exposure, respectively. At the end of the 6-week experiment, the animals were euthanized, the blood samples collected for some biochemical assays while the organs (kidney and liver) were harvested and subjected to histopathological examination. Results: From the biochemical assay, it was observed that the beetroot smoothies regulated and significantly reduced the elevated levels of AST, ALT, urea and creatinine observed in the animals that were exposed to dichlorvos. Additionally, the beetroot was able to regenerate the liver and kidney organs that were damaged on exposure to dichlorvos. Conclusion: This study concluded that beetroot smoothie possesses hepato-protective, hepato-curative as well as nephro-curative properties.

scholarly journals ISONIAZID (INH)

2018 ◽  
Vol 25 (10) ◽  
pp. 1587-1595
Author(s):  
Umer Aleem ◽  
Rahman Shah ◽  
Noor Khan ◽  
M. Suliman
Keyword(s):  
Serum Bilirubin ◽  
Statistical Significance ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group 2

Objectives: Hepatotoxicity is the most complicated side effect of isoniazid (inh)in the patient treated for tuberculosis. In causes 8–30% hepatotoxicity in the developing world.Metabolism of INH produces a metabolite, called acetyl isoniazid. In this study hepatoprotectiveeffect of honey, in isoniazid induced animal model was assessed. Study Design: Randomizedcontrol trial. Setting: Saidu Medical College, Saidu Sharif Swat, KP. Period: October ToDecember 2017. Material and Methods: 40 healthy male rabbits were assigned randomly tothe group i, ii, iii and iv by using lottery method. Ten animals were grouped each row. Theisoniazid-induced hepatotoxic model was created by giving 50 mg inh/kg orally on daily basisfor eleven days. Group i was taken as negative control group ii as a positive control. Group iii andiv were experimental groups treated with 50 mg /kg/day and 100 mg /kg/day buckwheat honeyrespectively for eleven days. SPSS Version 16 software was used, mean, s.d. were determinedin all the groups. Values of serum bilirubin, sgpt, and alkaline phosphatase were comparedwith each other using pairt-test. Results: SGPT, Serum bilirubin, and alkaline phosphatasewere obtained in all the animals. Comparing group 1 negative control with group 2, 3 and 4shows statistical significance, (p=0.00). Comparing group 2 positive control with 3 and 4 showsstatistical significance, (p=0.00). Further comparing group 3 with group 4 also shows statisticalsignificance (p=0.00). Conclusion: From the above finding, it has been revealed that honeyhas got a protective effect in regressing hepatitis that has been induced in rabbit’s model byhigh doses of isoniazid. Related studies performed in which different chemicals and drugs havebeen tried for their protective role in isoniazid induced hepatitis also shows a similar type ofresults.

scholarly journals ANTIINFLAMMATION ACTIVITY ETHANOL EXTRACT OF MARBOSI-BOSI LEAVES (Tarenna polycarpa (Miq.) Koord.Ex Valeton) ON WHITE RAT IN CARRAGEENAN INDUCED PAW INFLAMMATION

2018 ◽  
Vol 6 (3) ◽  
pp. 1-4
Author(s):  
Haris Munandarnst ◽  
Marline N
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Group Iii ◽  
Rat Paw Edema ◽  
Group I ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Traditionally, (Tarenna polycarpa (Miq.) Koord Ex Valeton as known as marbosi-bosi which commonly found in Sibolga, North Sumatera, Indonesia has been used as antidiabetes, cholesterol, anti-inflammatory and antibacterial. Tarenna species has been found its activities as antimicrobial, anti-oxidant and anti-inflammatory activity. The aim of this study was to investigate the anti inflammatory effect ethanol extract of marbosi-bosi leaves (Tarenna polycarpa (Miq.) Koord Ex Valeton in terms of decreased edema volume of male white rat in 1% carrageenan-induced and also to determine the effective dose of extract to decrease the volume of rat paw edema Ethanol extract of marbosi-bosi (Tarenna polycarpa (Miq.) Koord Ex Valeton was obtained by maceration. The antiinflammatory activity test was divided into 5 groups. The Group I (negative control) was given CMC 0.5%, Group II (positive control) was given diclofenac sodium 2,25 mg / kg BW, while Group III, IV and V were  given marbosi-bosi leaf extract at a dose of 50, 100 and 200 mg/kgBW respectively.  Each rat was induced by 1% carrageenan subplantar injection. Examination of antiinflammatory effect was measured by using digital plethysmometer at minute of 30 to minute of 360. The data were analyzed statistically using ANOVA (analysis of variance).The results showed that negative control did not show anti-inflammatory effect had significant differences with other treatment groups. In conclusion, ethanol extract marbosi-bosi (Tarenna polycarpa (Miq.) Koord Ex Valeton has an effective anti-inflammatory activity at a dose of 100 mg / kgBW.

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