scholarly journals Assessing the Viability of Commercial Media for the Mass Culture of Chaetoceros muelleri

2021 ◽  
pp. 201-209
Author(s):  
Rona Cabanayan-Soy ◽  
Glycinea de Peralta ◽  
Marie Antonette Juinio-Meñez
Keyword(s):  
Cell Density ◽  
Mass Culture ◽  
Culture Media ◽  
Exponential Growth Phase ◽  
Glass Bottle ◽  
Chaetoceros Muelleri ◽  
Commercial Media ◽  
Microalgal Culture ◽  
Glass Bottles ◽  
The Cost

The microalgae Chaetoceros muelleri is considered a highly nutritious feed for the cultured larvae of the tropical sea cucumber Holothuria scabra. Due to the cost of analytical grade culture media used in the production of C. muelleri, there is a need to evaluate cheap alternative commercial media to decrease the cost of producing quality live microalgal food. In this study, two different indoor batch culture systems (1 L glass bottles and 10 L plastic carboys) were used to evaluate the effectiveness of two conventional (modified F/2 and Walne’s) and one commercial (Epizyme AGP complete) microalgal culture media. Results of the 1 L glass bottle experiment showed that the peak cell density of C. muelleri in AGP (1,241 ± 116 x 104 cells ml-1) was not significantly different from the modified F/2 (1,584 ± 41 x 104 cells ml-1) and Walne’s medium (1,319 ± 162 x 104 cells ml-1) (Kruskal-Wallis test, p=0.78). Likewise, in the plastic carboy experiment, the maximum cell density of C. muelleri in Walne’s medium (750 ± 144 x 104 cells ml-1) and F/2 medium (653 ± 79 x 104 cells ml-1) were higher, but not significantly different from AGP (496 ± 184 x 104 cells ml-1) (Kruskal-Wallis test, p=0.43). The highest growth rate in the glass bottle cultures was the modified F/2 (0.38 div day-1), while AGP was the lowest (0.34 div. day-1). On the other hand, in carboy culture, AGP was higher (0.17 div.day-1) compared to modified F/2 (0.15 div. day-1) and Walne’s medium (0.13 div. day-1). The exponential growth phase was similar in the glass bottles, while in the carboy, the exponential phase was reached at a shorter time in the AGP treatment than those in the modified F/2 and Walne’s media. The findings showed that AGP medium is an adequate alternative to replace the conventional media (modified F/2 and Walne’s) during the secondary stock culture for C. muelleri. The viability of using cheaper and more readily available commercial AGP media for the indoor culture production of C. muelleri can contribute to cost-effective scaling-up of the hatchery production of quality H. scabra larvae and early juveniles.

scholarly journals Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species

2021 ◽  
Vol 105 (5) ◽  
pp. 2139-2156
Author(s):  
Justine Sauvage ◽  
Gary H. Wikfors ◽  
Xiaoxu Li ◽  
Mark Gluis ◽  
Nancy Nevejan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Culture Media ◽  
Fatty Acid Content ◽  
Pluronic F127 ◽  
Algal Culture ◽  
Omega 3 ◽  
Chaetoceros Muelleri ◽  
Omega 3 Fatty Acid ◽  
Live Feed ◽  
Chaetoceros Calcitrans

Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros calcitrans, Chaetoceros muelleri, Skeletonema costatum, and Thalassiosira pseudonana; two haptophytes-Tisochrysis lutea and Pavlova salina). Algal culture performance in response to the addition of NAC and pluronic, singly or combined, is dosage- and species-dependent. Combined NAC and pluronic F127 algal culture media additives resulted in specific growth rate increases of 38%, 16%, and 24% for C. calcitrans, C. muelleri, and P. salina, respectively. Enhanced culture productivity for strains belonging to the genus Chaetoceros was paired with an ~27% increase in stationary-phase cell density. For some of the species examined, culture media enrichments with NAC and pluronic resulted in increased omega-3-fatty acid content of the algal biomass. Larval development (i.e., growth and survival) of the Pacific oyster (Crassostrea gigas) was not changed when fed a mixture of microalgae grown in NAC- and F127-supplemented culture medium. Based upon these results, we propose that culture media enrichment with NAC and pluronic F127 is an effective and easily adopted approach to increase algal productivity and enhance the nutritional quality of marine microalgal strains commonly cultured for live-feed applications in aquaculture. Key points • Single and combined NAC and pluronic F127 culture media supplementation significantly enhanced the productivity of Chaetoceros calcitrans and Chaetoceros muelleri cultures. • Culture media enrichments with NAC and F127 can increase omega-3-fatty acid content of algal biomass. • Microalgae grown in NAC- and pluronic F127-supplemented culture media are suitable for live-feed applications.

Observation of High Speed Glass Bottle Fracture Behaviors by Underwater Shockwave

2012 ◽  
Vol 586 ◽  
pp. 151-155
Author(s):  
Kosuke Nagata ◽  
Hidetoshi Sakamoto ◽  
Yoshifumi Ohbuchi ◽  
Hiroyuki Kuramae ◽  
Eiji Nakamachi
Keyword(s):  
High Speed ◽  
Fracture Process ◽  
Glass Bottle ◽  
Explosive Energy ◽  
Washing Process ◽  
Fracture Behaviors ◽  
Glass Bottles ◽  
Glass Recycling

This paper described new effective glass bottle fracture process for glass recycling by underwater shockwave. The high-speed fracture behaviors of glass bottles by explosive energy were discussed. In the proposed technique, the washing process can be skipped because the bottle crushing process execute in water. As a result, the recycling cost can be decreased. In order to clarify the behaviors of glass bottle fracture, the bottle was painted by 5 colors. The crushing experiment was executed under four explosive conditions. The influence of various explosive conditions on the cullet sizes were calcified by using painted bottle.

A noble extended stochastic logistic model for cell proliferation with density-dependent parameters

2021 ◽  
Author(s):  
Trina Roy ◽  
Sinchan Ghosh ◽  
Bapi Saha ◽  
Sabyasachi Bhattacharya
Keyword(s):  
Cell Proliferation ◽  
Cell Density ◽  
Negative Feedback ◽  
Deterministic Model ◽  
Culture Media ◽  
Logistic Growth ◽  
Density Dependent ◽  
Proposed Model ◽  
Dependent Cell ◽  
Growth Inhibiting

Abstract Cell proliferation often experiences a density-dependent intrinsic proliferation rate (IPR) and negative feedback from growth-inhibiting molecules in culture media. The lack of flexible models with explanatory parameters fails to capture such a proliferation mechanism. We propose an extended logistic growth law with the density-dependent IPR and additional negative feedback. The extended parameters of the proposed model can be interpreted as density-dependent cell-cell cooperation and negative feedback on cell proliferation. Moreover, we incorporate further density regulation for flexibility in the model through environmental resistance on cells. The proposed growth law has similarities with the strong Allee model and harvesting phenomenon. We also develop the stochastic analog of the deterministic model by representing possible heterogeneity in growth-inhibiting molecules and environmental perturbation of the culture setup as correlated multiplicative and additive noises. The model provides a maximum sustainable stable cell density (MSSCD) and a new fitness measure for proliferative cells. The proposed model shows superiority to the logistic law after fitting to real cell culture datasets. We illustrate both MSSCD and the new cell fitness for a range of parameters. The cell density distributions reveal the chance of overproliferation, underproliferation, or decay for different parameter sets under the deterministic and stochastic setups.

scholarly journals Evaluation of Culture Media Limited in Nitrogen and Silicates on the Production Response and Lipid Content of the Diatom Chaetoceros muelleri

2014 ◽  
Vol 6 (4) ◽  
pp. 145-149 ◽  
Author(s):  
Lopez-Elias Jose Antonio ◽  
Carvallo Ruiz Maria Gisela ◽  
Estrada Raygoza Leticia Areli ◽  
Martinez Cordova Luis Rafael ◽  
Martinez-Porchas Marcel ◽  
...  
Keyword(s):  
Lipid Content ◽  
Culture Media ◽  
Chaetoceros Muelleri ◽  
Production Response

scholarly journals Sinking in Quicksand: The Demise of Victorian High Culture in America 1870-1915

2008 ◽  
Vol 3 ◽  
Author(s):  
Mitra Sharafi
Keyword(s):  
Mass Culture ◽  
Ethnic Origin ◽  
Turn Of The Century ◽  
Point Of View ◽  
Common Denominator ◽  
High Culture ◽  
Level Of Education ◽  
Modern Mass ◽  
The Cost ◽  
The Ideal

Today, the term Victorian implies snobbishness and rigidity. Our world, the result in part of a rebellion against Victorian formality and social hierarchy, celebrates the classless, the democratic, and the popular. It professes faith in the artistic judgment of all members of society regardless of ethnic origin, level of education or wealth. From the Victorian point of view, however, twentieth-century mass culture is accessible to all by appealing to the lowest common denominator; it is inclusive at the cost of a loss of education, refinement, and profundity. Turn-of-the-century America is the ideal subject for a study of the interaction between Victorian high culture and modern mass culture; the period from 1870 to 1915 was one of drastic cultural metamorphosis. Social change threatened the foundations of high culture and eventually killed it, but not without the unintentional help of the Victorians' own self-alienating behaviour.

New Technique for “Cullet” Generation of Glass Bottles by Using Underwater Shockwave

2006 ◽  
Vol 326-328 ◽  
pp. 1551-1554
Author(s):  
Hidetoshi Sakamoto ◽  
Shinjiro Kawabe ◽  
Kazuo Satoh ◽  
Masahiro Himeno ◽  
Shigeru Itoh
Keyword(s):  
Shock Wave ◽  
High Speed ◽  
Fracture Process ◽  
Image Converter ◽  
Glass Bottle ◽  
Underwater Shock Wave ◽  
Application Technique ◽  
System A ◽  
Underwater Shock ◽  
Glass Bottles

As the application technique for glass bottle’s recycling system, a new “Cullet” generation method by using underwater shock wave was proposed. This small fragmentation technique of glass bottles has a lot of excellent advantages such as the simplification of process by simultaneous cleaning and crushing operation, the high collect rate of “Cullet” and so on. In this study, the relation between of the explosive conditions and “Cullet” sizes were clarified and the high speed fracture process of glass bottle was observed by framing photograph of high speed image converter.

scholarly journals Improving the metabolic fidelity of cancer models with a physiological cell culture medium

2019 ◽  
Vol 5 (1) ◽  
pp. eaau7314 ◽  
Author(s):  
Johan Vande Voorde ◽  
Tobias Ackermann ◽  
Nadja Pfetzer ◽  
David Sumpton ◽  
Gillian Mackay ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cells ◽  
Culture Media ◽  
Hypoxia Inducible Factor ◽  
Commercial Media ◽  
Physiological Medium ◽  
Cancer Models ◽  
Cancer Spheroids

Currently available cell culture media may not reproduce the in vivo metabolic environment of tumors. To demonstrate this, we compared the effects of a new physiological medium, Plasmax, with commercial media. We prove that the disproportionate nutrient composition of commercial media imposes metabolic artifacts on cancer cells. Their supraphysiological concentrations of pyruvate stabilize hypoxia-inducible factor 1α in normoxia, thereby inducing a pseudohypoxic transcriptional program. In addition, their arginine concentrations reverse the urea cycle reaction catalyzed by argininosuccinate lyase, an effect not observed in vivo, and prevented by Plasmax in vitro. The capacity of cancer cells to form colonies in commercial media was impaired by lipid peroxidation and ferroptosis and was rescued by selenium present in Plasmax. Last, an untargeted metabolic comparison revealed that breast cancer spheroids grown in Plasmax approximate the metabolic profile of mammary tumors better. In conclusion, a physiological medium improves the metabolic fidelity and biological relevance of in vitro cancer models.

Rural Ambivalence Toward Mass Society: Evidence from the U.S. Parcel Post Debates, 1900–1913

Rural History ◽  
1994 ◽  
Vol 5 (1) ◽  
pp. 81-102 ◽  
Author(s):  
Richard B. Kielbowicz
Keyword(s):  
Mass Culture ◽  
Consumer Choice ◽  
Mass Communication ◽  
Small Towns ◽  
Turn Of The Century ◽  
Consumer Society ◽  
Mass Society ◽  
Production And Distribution ◽  
The Media ◽  
The Cost

For rural Americans, the debate over establishing a parcel post evoked all the hopes and anxieties associated with the expansion of mass society at the turn of the century. Parcel post, today an accepted and seemingly inconsequential government service, was originally seen as a linchpin in the emerging industry of mass culture. The media of mass communication advertised products and ran stories acclimating readers to a consumer society, thereby encouraging demand for mass-produced goods that were distributed, finally, by parcel post. Opponents of parcel post foresaw a decline of small towns, a centralization of production and distribution, a disruption of the ‘natural’ relations among labor, retailers, and consumers, and the aggrandizement of urban culture. At the other extreme, proponents claimed that parcel post would increase consumer choice, reduce the cost of living, and bridge the widening chasm between urban and rural life. Thus, the simple act of carrying a parcel from Chicago to a farmer's lane became freighted with a panoply of issues agitating the nation.

scholarly journals Sociality in Escherichia coli: Enterochelin Is a Private Good at Low Cell Density and Can Be Shared at High Cell Density

2015 ◽  
Vol 197 (13) ◽  
pp. 2122-2128 ◽  
Author(s):  
Rebecca L. Scholz ◽  
E. Peter Greenberg
Keyword(s):  
Escherichia Coli ◽  
Cell Density ◽  
Iron Acquisition ◽  
Partial Privatization ◽  
High Cell ◽  
Wild Type ◽  
Content Type ◽  
Cell Densities ◽  
The Cost ◽  
Low Cell Density

ABSTRACTMany bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of production. Here we show that theEscherichia colisiderophore enterochelin is not evenly shared between producers and nonproducers. Wild-typeEscherichia coligrows well in low-iron minimal medium, and an isogenic enterochelin synthesis mutant (ΔentF) grows very poorly. The enterochelin mutant grows well in low-iron medium supplemented with enterochelin. At high cell densities the ΔentFmutant can compete equally with the wild type in low-iron medium. At low cell densities the ΔentFmutant cannot compete. Furthermore, the growth rate of the wild type is unaffected by cell density. The wild type grows well in low-iron medium even at very low starting densities. Our experiments support a model where at least some enterochelin remains associated with the cells that produce it, and the cell-associated enterochelin enables iron acquisition even at very low cell density. Enterochelin that is not retained by producing cells at low density is lost to dilution. At high cell densities, cell-free enterochelin can accumulate and be shared by all cells in the group. Partial privatization is a solution to the problem of iron acquisition in low-iron, low-cell-density habitats. Cell-free enterochelin allows for iron scavenging at a distance at higher population densities. Our findings shed light on the conditions under which freeloaders might benefit from enterochelin uptake systems.IMPORTANCESociality in microbes has become a topic of great interest. One facet of sociality is the sharing of secreted products, such as the iron-scavenging siderophores. We present evidence that theEscherichia colisiderophore enterochelin is relatively inexpensive to produce and is partially privatized such that it can be efficiently shared only at high producer cell densities. At low cell densities, cell-free enterochelin is scarce and only enterochelin producers are able to grow in low-iron medium. Because freely shared products can be exploited by freeloaders, this partial privatization may help explain how enterochelin production is stabilized inE. coliand may provide insight into when enterochelin is available for freeloaders.

scholarly journals Comparative evaluation of three different commercial blood culture media for recovery of anaerobic organisms

1977 ◽  
Vol 5 (5) ◽  
pp. 505-509
Author(s):  
J I Mangels ◽  
L H Lindberg ◽  
K L Vosti
Keyword(s):  
Blood Culture ◽  
Comparative Evaluation ◽  
Culture Media ◽  
Anaerobic Bacteria ◽  
Brain Heart Infusion ◽  
Blood Stream ◽  
Becton Dickinson ◽  
Commercial Media ◽  
The Brain ◽  
Peptone Broth

A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in a Vacutainer tube with added CO2 (Becton-Dickinson). During a period of 17 consecutive months, 12,216 specimens of blood were processed with each broth. Aerobic or anaerobic bacteria were recovered from 913 specimens (7%). Seventy-four specimens (8%) of the total positive cultures contained anaerobic organisms. When potential contaminants were removed from the totals, 7% of the positive cultures contained anaerobic organisms and 7% of the patients with positive cultures had bacteremia with anaerobic bacteria. Of the three commercial blood culture media studied, the prereduced, supplemented peptone broth recovered more anaerobic organisms than did either the brain heart infusion or Thiol broths.

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