scholarly journals PREPARATION OF THE ELECTROSPUN POLYVINYLIDENE FLUORIDE / POLYVINYL ALCOHOL SCAFFOLD AS A POTENTIAL TISSUE REPLACEMENT

2021 ◽  
Vol 22 (1) ◽  
pp. 245-258
Author(s):  
Mohd Syahir Anwar Hamzah ◽  
Nurul Amira Ab Razak ◽  
Celine Ng ◽  
Akmal Hafiszi Abdul Azize ◽  
Jumadi Abdul Sukor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyvinyl Alcohol ◽  
Polyvinylidene Fluoride ◽  
Electrospinning Process ◽  
Hydroxyl Group ◽  
Tissue Scaffold ◽  
Tissue Replacement ◽  
Bonding Interaction ◽  
Artificial Tissue

:  Polyvinylidene fluoride (PVDF), a piezoelectric material, is commonly used in tissue engineering due to its potential for mimicking the electrical microenvironment of biological conditions for tissue development. In this present research, polyvinyl alcohol (PVA) was introduced into electrospun PVDF fabrication through an electrospinning process, aiming to enhance the nanofibrous membrane's biocompatibility properties by improving the hydrophilicity properties to act as an artificial tissue scaffold. The electrospun PVDF/PVA membranes are found to be optimum at a PVDF-to-PVA ratio of 90:10 due to its excellent mechanical, morphological, and hydrophilicity conductivity properties. Fourier transform infrared (FTIR) spectroscopy verified strong hydrogen bonding interaction formed between the fluorine group of PVDF with oxygen-containing in the hydroxyl group of PVA. In-vitro cell culture showed that the enhanced hydrophilic property of electrospun PVDF/PVA could significantly enhance the cell growth. These positive results indicated that the scaffold could be implemented as artificial tissue material for tissue engineering applications. ABSTRAK: Polivinilidena fluorida (PVDF) adalah bahan piezoelektrik yang biasa digunakan dalam kejuruteraan tisu kerana potensinya menyerupai keadaan persekitaran mikro-elektrik biologi bagi perkembangan tisu. Dalam penyelidikan ini, polivinil alkohol (PVA) diperkenalkan ke dalam fabrikasi pintalan-elektro PVDF melalui proses pemintalan-elektro, yang bertujuan bagi mengembangkan sifat biokompatibiliti membran nanogentian dengan meningkatkan sifat hidrofilik bagi menjadi perancah tisu tiruan. Membran pintalan-elektro PVDF / PVA didapati optimum pada nisbah PVDF-ke-PVA, 90:10 kerana sifat kekonduksian, mekanikal, morfologi dan hidrofiliknya yang sangat baik. Spektroskopi transformasi inframerah Fourier (FTIR) mengesahkan interaksi ikatan hidrogen yang kuat terbentuk antara kumpulan fluoro PVDF dengan oksigen yang terkandung dalam kumpulan hidroksil PVA. Kultur sel secara in-vitro menunjukkan bahawa sifat hidrofilik pintalan-elektro PVDF / PVA dapat meningkatkan pertumbuhan sel secara signifikan. Hasil positif ini menunjukkan bahawa perancah ini dapat digunakan sebagai bahan tisu buatan bagi aplikasi kejuruteraan tisu.

scholarly journals Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Stefano Focaroli ◽  
Gabriella Teti ◽  
Viviana Salvatore ◽  
Isabella Orienti ◽  
Mirella Falconi
Keyword(s):  
Tissue Engineering ◽  
Bone Morphogenetic Proteins ◽  
Transforming Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Biomechanical Properties ◽  
Alginate Beads ◽  
Cartilage Tissue ◽  
Self Healing ◽  
Tissue Replacement

Articular cartilage is a highly organized tissue with complex biomechanical properties. However, injuries to the cartilage usually lead to numerous health concerns and often culminate in disabling symptoms, due to the poor intrinsic capacity of this tissue for self-healing. Although various approaches are proposed for the regeneration of cartilage, its repair still represents an enormous challenge for orthopedic surgeons. The field of tissue engineering currently offers some of the most promising strategies for cartilage restoration, in which assorted biomaterials and cell-based therapies are combined to develop new therapeutic regimens for tissue replacement. The current study describes thein vitrobehavior of human adipose-derived mesenchymal stem cells (hADSCs) encapsulated within calcium/cobalt (Ca/Co) alginate beads. These novel chondrogenesis-promoting scaffolds take advantage of the synergy between the alginate matrix and Co+2ions, without employing costly growth factors (e.g., transforming growth factor betas (TGF-βs) or bone morphogenetic proteins (BMPs)) to direct hADSC differentiation into cartilage-producing chondrocytes.

scholarly journals Electrospun Polyvinylidene Fluoride-Based Fibrous Scaffolds with Piezoelectric Characteristics for Bone and Neural Tissue Engineering

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 952 ◽  
Author(s):  
Li ◽  
Liao ◽  
Tjong
Keyword(s):  
Tissue Engineering ◽  
Polyvinylidene Fluoride ◽  
Neural Tissue ◽  
Electrospinning Process ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Engineering Applications ◽  
Pore Sizes ◽  
Β Phase ◽  
Small Pore

Polyvinylidene fluoride (PVDF) and polyvinylidene fluoride-trifluoroethylene (P(VDF-TrFE) with excellent piezoelectricity and good biocompatibility are attractive materials for making functional scaffolds for bone and neural tissue engineering applications. Electrospun PVDF and P(VDF-TrFE) scaffolds can produce electrical charges during mechanical deformation, which can provide necessary stimulation for repairing bone defects and damaged nerve cells. As such, these fibrous mats promote the adhesion, proliferation and differentiation of bone and neural cells on their surfaces. Furthermore, aligned PVDF and P(VDF-TrFE) fibrous mats can enhance neurite growth along the fiber orientation direction. These beneficial effects derive from the formation of electroactive, polar β-phase having piezoelectric properties. Polar β-phase can be induced in the PVDF fibers as a result of the polymer jet stretching and electrical poling during electrospinning. Moreover, the incorporation of TrFE monomer into PVDF can stabilize the β-phase without mechanical stretching or electrical poling. The main drawbacks of electrospinning process for making piezoelectric PVDF-based scaffolds are their small pore sizes and the use of highly toxic organic solvents. The small pore sizes prevent the infiltration of bone and neuronal cells into the scaffolds, leading to the formation of a single cell layer on the scaffold surfaces. Accordingly, modified electrospinning methods such as melt-electrospinning and near-field electrospinning have been explored by the researchers to tackle this issue. This article reviews recent development strategies, achievements and major challenges of electrospun PVDF and P(VDF-TrFE) scaffolds for tissue engineering applications.

Preparation of Electrospun PLA Nanofiber Scaffold and the Evaluation In Vitro

2005 ◽  
Vol 288-289 ◽  
pp. 139-142 ◽  
Author(s):  
Xian Tao Wen ◽  
Hong Song Fan ◽  
Yan Fei Tan ◽  
H.D. Cao ◽  
H. Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
High Surface ◽  
Volume Ratio ◽  
Electrospinning Process ◽  
High Porosity ◽  
Electrospun Scaffold ◽  
Nanofiber Scaffold ◽  
Soft Tissue Engineering

A electrospinning process to prepare soft tissue engineering scaffold was introduced in this study. This kind of scaffold was composed with ultrathin fiber and characterized with high porosity, well-interconnected pores and high surface-to-volume ratio. Biodegradable polylaticacid (PLA) was used to spin the scaffold and the scaffold was evaluated in vitro by analysis the microscopic structure, porosity, mechanical property, especially cytocompatibility. The results indicated that the electrospun PLA scaffold showed good cytocompatibility and the tensile property of electrospun scaffold was similar to human’s soft tissue. It could be expected that the electrospun scaffold would be potential in soft tissue engineering or soft tissue repair.

Biomineralization of Electrospun Nano-HA/PHB GTR Membrane

2007 ◽  
Vol 330-332 ◽  
pp. 695-698 ◽  
Author(s):  
Dong Hua Guan ◽  
Chun Peng Huang ◽  
Ji Liu ◽  
Kun Tian ◽  
Lin Niu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Electrospinning Process ◽  
Nanometer Scale ◽  
High Porosity ◽  
The Novel ◽  
Mineral Crystal ◽  
Novel Technology ◽  
Air Jet

Poly 3-hydroxybutyrate (PHB) as a kind of polysaccharides has been proved promising for tissue engineering because of its biocompatibility and biodegradability. But its poor mechanical properties and hydrophilicity limit its application. In order to explore a new useful porch to improve the performance of PHB-based GTR membrane, membrane composed of nano-HA / PHB composite was manufactured through the air/jet electrospinning process which can potentially generate nanometer scale diameter fibers and enlarge surface area of materials while maintaining high porosity. Successively, the biomineralization behavior of the membrane in supersaturated calcification solution (SCS) was studied. The Results of this investigation show that the successfully manufactured porous nano-HA/PHB membrane has high activity in SCS and its ability of inducing the formation of mineral crystal in vitro than that of the unfilled PHB membrane. It can be concluded that the addition of nano-HA and the novel technology could improve the performance of the PHB-based GTR membrane.

Tailoring in vitro biological and mechanical properties of polyvinyl alcohol reinforced with threshold carbon nanotube concentration for improved cellular response

RSC Advances ◽  
2016 ◽  
Vol 6 (46) ◽  
pp. 39982-39992 ◽  
Author(s):  
Tejinder Kaur ◽  
Arunachalam Thirugnanam
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Polyvinyl Alcohol ◽  
Bone Tissue ◽  
Cellular Response ◽  
Natural Bone ◽  
Tissue Constructs ◽  
Living Bone

The development of living bone tissue constructs with structural, mechanical and functional similarities to natural bone are the major challenges in bone tissue engineering.

scholarly journals Synergistic effects of retinoic acid and graphene oxide on the physicochemical and in-vitro properties of electrospun polyurethane scaffolds for bone tissue engineering

e-Polymers ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 363-371 ◽  
Author(s):  
Mohammad Mahdi Safikhani ◽  
Ali Zamanian ◽  
Farnaz Ghorbani
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Retinoic Acid ◽  
Synergistic Effects ◽  
Biological Properties ◽  
Electrospinning Process ◽  
Hard Tissue ◽  
Tissue Engineering Scaffolds ◽  
Sem Micrographs

AbstractTissue engineering scaffolds simulate extracellular matrixes (ECMs) to promote healing processes of damaged tissues. In this investigation, ECM were simulated by retinoic acid-loaded polyurethane-graphene oxide nanofibers to regenerate bone defects. Scanning electron microscopy (SEM) micrographs, Fourier transform infrared (FTIR) spectrum and X-ray diffraction (XRD) patterns proved the synthesis of graphene oxide (GO) nanosheets. SEM micrographs of nanofibers demonstrated through the formation of homogeneous and bead free fibrous scaffolds that the diameter of fibers were reduced by decreasing the applied voltage in an electrospinning process and the addition of GO. According to the results, the addition of GO to the polyurethane (PU) solution led to an increase in mechanical strength which is the most important parameter in the hard tissue repair. The GO-containing scaffolds showed an increased wettability, swelling, biodegradation and drug release level. Release behavior in nanocomposite scaffolds followed the swelling and biodegradation mechanisms, so osteogenic expression was possible by incorporating retinoic acid (RA) in PU-GO nanofibrous scaffolds. Biological evaluations demonstrated that composite scaffolds are biocompatible and support cellular attachment in which RA-loaded samples represented better cellular spreading. In brief, nanocomposite fibers showed desired that the physicochemical, mechanical and biological properties and synergic effects of GO and RA in osteogenic activity of MG-63 cells produced favorable constructs for hard tissue engineering applications.

Defect-related luminescent microstructured hydroxyapatite promote bone regeneration through nucleating effect

2020 ◽  
Vol 10 (7) ◽  
pp. 1102-1108
Author(s):  
Chunyan Dai ◽  
Qian Wang ◽  
Georgios Patias ◽  
Ataulla Shegiwal ◽  
Linhua Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Differentiation ◽  
Bone Regeneration ◽  
Collagen Synthesis ◽  
Tissue Scaffold ◽  
Micro Scale ◽  
Nucleating Effect

Non-nano scaled hydroxyapatite (HAP) particles cannot enter the cells, but they are also wildly used in tissue engineering for their excellent bone regeneration. We synthesized a defect-related luminescent micro-scale hydroxyapatite particles (S3) and investigated the effect of S3 during bone regeneration. S3 promoted the formation of mineralized nodules and collagen synthesis of osteoblasts (OBs). Micro-scaled S3 couldn't enter into OBs and couldn't change the Ca2+ concentration of the medium. During the cell differentiation, the location of S3 was tracked by its defect-related luminescence in vitro. Extracellular S3 particles become the nucleation events which promote bone regeneration. The results suggest that micro-scale HAP promoted bone regeneration through extracellular pathways. This result also can explain the reason why hydroxyapatite covered tissue scaffold is more suitable for bone reconstructing.

scholarly journals 3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

2020 ◽  
Vol 21 (20) ◽  
pp. 7757
Author(s):  
Jongmin Kim ◽  
Jeong Sik Kong ◽  
Wonil Han ◽  
Byoung Soo Kim ◽  
Dong-Woo Cho
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
In Vitro Testing ◽  
Living Tissue ◽  
Cell Printing ◽  
Artificial Tissue ◽  
Engineered Tissue ◽  
Pharmaceutical Industries

The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

Graphene oxide-enriched poly(ε-caprolactone) electrospun nanocomposite scaffold for bone tissue engineering applications

2016 ◽  
Vol 32 (3) ◽  
pp. 325-342 ◽  
Author(s):  
Sepideh Mohammadi ◽  
Seyedeh Sara Shafiei ◽  
Mitra Asadi-Eydivand ◽  
Mahmoud Ardeshir ◽  
Mehran Solati-Hashjin
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Average Diameter ◽  
Electrospinning Process ◽  
Graphene Oxide Nanosheets ◽  
Mg63 Cells ◽  
Graphene Oxide Nanosheet ◽  
Nanocomposite Scaffolds ◽  
Pure Poly

Tissue engineering aims at fabricating biological substitutes to improve, repair, and regenerate failing human tissues or organs. Designing a nanocomposite scaffolds with tailored properties that enhance the development of functional tissue can be an appropriate approach to achieve this purpose. In this study, the uniform and bead-free nanofibers of poly(ε-caprolactone) composited with different graphene oxide nanosheet contents (ranging from 0.5 to 2 wt%) were successfully fabricated through electrospinning process. A decrease in the average diameter of poly(ε-caprolactone) nanofibers was observed with the addition of graphene oxide nanosheets. Moreover, the nanocomposite scaffolds containing 2 wt% of graphene oxide nanosheets exhibited superior mechanical properties compared to that of pure poly(ε-caprolactone). Compared with pure poly(ε-caprolactone) scaffold, the degradation rate of poly(ε-caprolactone)-graphene oxide nanosheet nanofibers was enhanced, while the integrity of fibers was preserved. The presence of graphene oxide nanosheets in poly(ε-caprolactone) fibers promoted in vitro biomineralization, indicating bioactive features of the nanocomposite scaffolds. Compared to the pure one, nanocomposite fibers also showed better ability in protein adsorption. The in vitro cell culture studies showed that the addition of graphene oxide nanosheets did not diminish the biocompatibility of the electrospun poly(ε-caprolactone) nanofiber. Furthermore, the adhesion and proliferation of MG63 cells were increased. Altogether, the results demonstrated that electrospun poly(ε-caprolactone)-graphene oxide nanosheet nanofiber may be a suitable candidate for tissue engineering scaffold applications.

Methods for Achieving Soft Tissue Scaffold Sterility

2009 ◽  
Vol 4 ◽  
pp. 59-69 ◽  
Author(s):  
Alex Baume ◽  
Nick Coleman ◽  
Philip Boughton
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Medical Devices ◽  
Tissue Engineering Scaffold ◽  
Test Method ◽  
Mechanical Agitation ◽  
Packaging System ◽  
Tissue Scaffold ◽  
And Performance

The manufacturing process for in vitro tissue culture products and medical devices relies on a validated sterilization route for ensuring product sterility, safety and performance. Two key aspects that contribute toward final sterilization validation are (1) the reliable estimation of product bioburden and (2) the development of a proficient sterile packaging system. Bioabsorbable composite systems and architecture of tissue scaffolds can lead to numerous challenges for bioburden testing and packaging design. This study is concerned with the development of bioburden assessment methods and packaging systems for Variotis™; a soft tissue engineering scaffold. A bioburden test method relying on mechanical agitation was established. Bioburden assessment was achieved by recovering Geobacillus stearothermophilus spore inoculant for analysis. A packaging system was developed which provides adequate protection for Variotis™ scaffolds while meeting other user/sterilization requirements for research grade product. The guidelines and design approaches included in this study are generally applicable to other tissue engineering scaffold and medical devices.

Sign in / Sign up

Export Citation Format

Share Document