scholarly journals Periodic Assessment Of Antenatal And Post Natal Serum Endothelin1 and Nitric Oxide Levels In Hypertensive Disorders Of Pregnancy

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Hidayatul Radziah Ismawi ◽  
Tariq Abdul Razaq ◽  
Maizura Mohd. Zainudin ◽  
Nur Jasmine Aida Jamani
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Endothelial Dysfunction ◽  
Pregnant Women ◽  
Post Partum ◽  
Chronic Hypertension ◽  
Hypertensive Disorder ◽  
Sample Collection ◽  
Endothelin 1 ◽  
Potent Vasoconstrictor

Introduction: Hypertensive Disorder of Pregnancy (HDP) is an independent risk factor of cardiovascular (CVS) disease with persistent endothelial dysfunction, that occurs after the initial insult during pregnancy and persists post partum postulated to be the pathophysiology. Endothelin-1 (ET-1), a potent vasoconstrictor, has been identified as a pivotal mediator in both essential hypertension and HDP. Disturbances in Nitric Oxide (NO) bioavailability found in endothelial dysfunction may increase susceptibility to cardiovascular diseases such as hypertension. Materials and Methods: Thirty six pregnant women at 30-36 weeks period of gestation from the following categories (i) pregnancy induced hypertension (PIH) (ii) chronic hypertension during pregnancy (CH) and (iii) normal pregnant women (Control). Blood pressure indices measurements and sample collection was done at antepartum (30-36 weeks), post partum (8 weeks and 12 weeks). Endothelin-1 was measured using the Human ET-1 (Endothelin-1) ELISA Kit and serum NO was measured using the Nitric Oxide (total) detection kit. Results: All blood pressure indices were significantly higher in HDP patients compared to control during antenatal and post partum periods. Serum ET-1 was significantly higher in patients with HDP compared to control during antenatal until 3 months post partum. This was accompanied by significantly lower levels of serum NO in HDP patients. Conclusion: ET-1 levels in patients with history of HDP irrespective of post partum blood pressure contributes to persistent endothelial dysfunction.

scholarly journals Periodic Assessment of Antenatal and Post Natal Serum Endothelin-1 (ET-1) and Nitric Oxide (NO) Levels in Hypertensive Disorders of Pregnancy (HDP)

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Hidayah I ◽  
Tariq A.R. ◽  
N.A. Jamani ◽  
Maizura M.Z.
Keyword(s):  
Nitric Oxide ◽  
Endothelial Dysfunction ◽  
Pregnant Women ◽  
Disease Risk ◽  
Chronic Hypertension ◽  
Hypertensive Disorders Of Pregnancy ◽  
Sample Collection ◽  
Endothelin 1 ◽  
Hypertensive Disorders ◽  
Potent Vasoconstrictor

Hypertensive Disorders of Pregnancy (HDP) is an independent risk factor of cardiovascular (CVS) disease with endothelial dysfunction postulated to be the pathophysiology. Endothelin-1 (ET-1), a potent vasoconstrictor, has been identified as a pivotal mediator in HDP. Disturbances in nitric oxide (NO) bioavailability found in endothelial dysfunction may increase susceptibility to cardiovascular diseases such as hypertension. The study aims to determine serial ET-1 and NO levels in patients with HDP and its role in persistent endothelial dysfunction. Thirty-six pregnant women from the following categories (i) normal pregnant women (Control) (ii) chronic hypertension during pregnancy (CH) and (iii) pregnancy induced hypertension (PIH) participated in this study. Blood pressure indices measurements and sample collection were done at antepartum (32 weeks) and postpartum (8 weeks and 12 weeks). ET-1 and serum NO were measured using the Human ET-1 (Endothelin-1) ELISA Kit and Nitric Oxide (total) detection kit respectively. Results: Serum ET-1 was significantly higher in patients with CH (55.3 pg/ml) and PIH (35.6 pg/ml) compared to Control (11.8 pg/ml) during antenatal until 3 months postpartum (CH 38.3 pg/ml, PIH 29.5 pg/ml, Control 1.9 pg/ml). This was accompanied by significantly lower levels of serum NO in HDP patients. Persistently higher than normal levels of ET-1 and lower than normal levels of NO up to 3 months postpartum in patients with history of HDP indicate presence of persistent endothelial dysfunction despite BP normalisation in PIH patients. Long term NO/ET-1 imbalance may account for the increased CVS disease risk.

scholarly journals Altered Bioavailability of Nitric Oxide and L-Arginine Is a Key Determinant of Endothelial Dysfunction in Preeclampsia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Worlanyo Tashie ◽  
Linda Ahenkorah Fondjo ◽  
William K. B. A. Owiredu ◽  
Richard K. D. Ephraim ◽  
Listowell Asare ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Endothelial Dysfunction ◽  
Pregnant Women ◽  
Positive Association ◽  
Neonatal Morbidity ◽  
Sub Saharan Africa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Birth Weight Infants ◽  
Significant Positive Association

Background. Preeclampsia is a major cause of maternal and neonatal morbidity and mortality in sub-Saharan Africa. Evidence indicates that endothelial dysfunction is central to the pathogenesis of preeclampsia. This study assessed the level of the components of the arginine-nitric oxide pathway to evaluate endothelial dysfunction in normotensive pregnancies and pregnancies complicated with preeclampsia. Methods. This case-control study was conducted among pregnant women who visited Comboni Hospital from January 2017 to May 2018. A total of 180 pregnant women comprising 88 preeclamptic women (PE) and 92 healthy normotensive pregnant women (NP) were recruited. Sociodemographic, clinical, and obstetric data were obtained using validated questionnaires. Blood pressure and anthropometrics were measured, and blood samples were collected for the estimation of nitric oxide (NO∙), L-arginine, asymmetric dimethylarginine (ADMA), and 3-nitrotyrosine using an enzyme-linked immunosorbent assay technique. Results. The mean NO∙ ( p = 0.010 ) and L-arginine/ADMA ratio ( p < 0.0001 ) was significantly lower in PE compared to NP while mean L-arginine ( p = 0.034 ), ADMA ( p < 0.0001 ), and 3-nitrotyrosine ( p < 0.0001 ) were significantly higher in PE than NP. ADMA showed a significant positive association with systolic blood pressure ( β = 0.454 , p = 0.036 ) in severe PE. Women with PE had significant intrauterine growth restriction ( p < 0.0001 ) and low birth weight infants ( p < 0.0001 ) when compared to NP. Conclusion. Preeclampsia is associated with reduced NO∙ bioavailability, L-arginine/ADMA ratio, and elevated levels of ADMA and 3-nitrotyrosine. Measurements of the levels of these parameters can help in the early prediction of endothelial dysfunction in preeclampsia. Exogenous therapeutic supplementation with L-arginine during pregnancy to increase the L-arginine/ADMA ratio should be considered to improve endothelial function in preeclampsia and pregnant women at risk of developing preeclampsia.

Associations of endothelial biomarkers, nitric oxide metabolites and endothelin, with blood pressure and coronary lesions depend on cardiovascular risk and sex to mark endothelial dysfunction on the SCORE scale

2020 ◽  
Vol 41 (4) ◽  
Author(s):  
Nadezhda G. Gumanova ◽  
Alexander U. Gorshkov ◽  
Marina V. Klimushina ◽  
Alexander Y. Kots
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Cardiovascular Risk ◽  
Endothelial Dysfunction ◽  
Coronary Atherosclerosis ◽  
Risk Estimation ◽  
High Systolic Blood Pressure ◽  
Endothelin 1 ◽  
Coronary Lesions ◽  
Circulating Levels

AbstractObjectivesEndothelial dysfunction contributes to the onset and progression of cardiovascular diseases. However, direct associations of vasoactive mediators with cardiovascular risk are poorly understood.MethodsWe have determined associations of circulating levels of stable metabolites of nitric oxide, nitrate and nitrite (NOx), endothelin-1, and the endothelin-1/NOx ratio with blood pressure in 177 asymptomatic subjects without signs of coronary atherosclerosis; associations with blood pressure and with presence of coronary lesions were also evaluated in 457 patients suspected to have coronary heart disease with or without coronary lesions confirmed by coronary angiography. All participants were on a low nitrate diet 24 h prior to blood sampling.ResultsIn men, NOx levels were inversely correlated with blood pressure similar to women with low (0–4%) European Systematic Coronary Risk Estimation (SCORE). However, the correlation was not significant in women with high SCORE (5–8%). High systolic blood pressure over 140 mm Hg was negatively associated with NOx levels in asymptomatic men (p=0.05) but not in women. This association is disrupted in male and female patients with coronary atherosclerosis. In male patients, NOx (p=0.05), endothelin (p=0.01), and the endothelin/NOx ratio (p=0.04) were associated with presence of coronary lesions.ConclusionsThus, elevated cardiovascular risk according to SCORE over 4% in asymptomatic women, but not in men, is associated with a shift in markers of endothelial dysfunction. Presence of coronary lesions in patients is associated with significant changes in circulating levels of markers of endothelial dysfunction in men but not in women.

Coupling of Heart Rate and Systolic Blood Pressure in Hypertensive Pregnancy

2014 ◽  
Vol 53 (04) ◽  
pp. 286-290 ◽  
Author(s):  
C. Fischer ◽  
R. Schroeder ◽  
A. Voss
Keyword(s):  
Blood Pressure ◽  
Risk Stratification ◽  
Systolic Blood Pressure ◽  
Pregnant Women ◽  
Gestational Hypertension ◽  
Characteristic Curve ◽  
Chronic Hypertension ◽  
Hypertensive Disorder ◽  
Hypertensive Pregnancy ◽  
Pregnancy Disorders

SummaryIntroduction: This article is part of the Focus Theme of Methods of Information in Medicine on “Biosignal Interpretation: Advanced Methods for Studying Cardiovascular and Respiratory Systems”.Background: Hypertensive pregnancy disorders affect 6 – 8 percent of all pregnancies and can result in severe complications for both the mother and the fetus.Objectives: The aim of this study was to improve risk stratification of pregnant women suffering from hypertension and pre-eclampsia (PE) by applying bivariate Segmented Poincaré plot analysis (BSPPA).Methods: From 35 pregnant women suffering from chronic hypertension, gestational hypertension and PE, 30 minutes of non invasive systolic blood pressure and beat-to-beat intervals were continuously recorded and analyzed by applying BSPPA to quantify their couplings.Results: We revealed significant different couplings between chronic hypertension (CH), gestational hypertension and PE, indicating that cardiovascular regulation can be considerably altered depending on the type of hypertensive disorder. The optimal multivariate set of two BSPPA indices was determined which distinguish best between CH and PE. It achieved a sensitivity of 100%, a specificity of 77.8% and an area under the receiver operator characteristic curve of 90.8%.Conclusions: The BSPPA method a) provides improved risk stratification for pregnant women suffering from hypertension and PE, b) increases the ability to diagnose pathological changes, and c) could contribute substantially to the differential diagnosis of hypertensive pregnancy disorders.

Nitric Oxide in Different Types of Hypertension during Pregnancy

1997 ◽  
Vol 93 (5) ◽  
pp. 413-421 ◽  
Author(s):  
Jenny V. Garmendia ◽  
Ylse Gutiérrez ◽  
Isaac Blanca ◽  
N. E. Bianco ◽  
J. B. De Sanctis
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Platelet Count ◽  
Pregnant Women ◽  
Normal Pregnancy ◽  
Chronic Hypertension ◽  
Different Types ◽  
History Of ◽  
Family History Of Hypertension ◽  
Positive Correlations

1. Serum nitric oxide (NO) levels (determined by its products of oxidation) were assessed in nonpregnant women, normal pregnant women and patients suffering from mild pre-eclampsia (MPE), severe pre-eclampsia (SPE), chronic hypertension (CHT) and CHT with pre-eclampsia (CHT+PE). The levels of NO products were significantly reduced during pregnancy in MPE (P < 0.001), CHT+PE (P < 0.01) and SPE (P < 0.05). Significant reductions of NO products were also observed in puerperium (P < 0.001) in all groups except CHT+PE (P < 0.05). 2. In normal pregnancy, three events were related to NO levels: (1) negative correlations were found between the levels of nitrite (r = −0.73, P = 0.0003), nitrate (r = −0.53, P = 0.017) and the number of weeks of gestation; (2) in the caesarean section group, the levels of NO at puerperium were significantly lower (P < 0.05) than those during pregnancy; and (3) there was a significant reduction in NO levels in the pregnant women carrying male fetuses as compared with female fetuses (P < 0.05). 3. In SPE, the patients with a family history of hypertension had lower levels of NO compared with the patients without such a history (P < 0.05). 4. A negative correlation was observed between systolic blood pressure, diastolic blood pressure and NO levels in MPE (r = −0.62, P = 0.013 and r = −0.68, P = 0.0049 respectively) and SPE (r = −0.72, P = 0.004 and r = −0.53, P = 0.037 respectively). 5. In SPE, positive correlations were observed between platelet count and nitrite (r = 0.67, P = 0.006) and nitrate levels (r = 0.56, P = 0.028). 6. In MPE, patients with anti-hypertensive treatment showed significantly (P < 0.05) higher levels of NO compared with the non-treated patients. 7. NO may be important in the physiopathology of hypertension during pregnancy, although several factors may affect its levels.

scholarly journals Assessment of Risk Factors in Hypertensive Disorder During Pregnancy: Hospital Based (KAP) Knowledge, Attitude and Practice Survey. A Key for Preventive Approach

2018 ◽  
Vol 16 (2) ◽  
pp. 74-77
Author(s):  
Merina Shrestha
Keyword(s):  
Risk Factors ◽  
Blood Pressure ◽  
Pregnant Women ◽  
Gestational Hypertension ◽  
Cross Sectional Study ◽  
Chronic Hypertension ◽  
Hypertensive Disorder ◽  
Cross Sectional ◽  
Knowledge And Attitude ◽  
Associated Risk Factors

Background: Hypertensive disorder (HDP) during pregnancy is a group of conditions featuring with high blood pressure that may lead to feto-maternal morbidity and mortality, particularly in developing countries. Aims and Objectives: To assess relationship between risk factor of hypertensive disorder during pregnancy, their self care knowledge and practice to prevent and control hypertension. Materials And Method: A cross-sectional study of 93 registered, pregnant women belonging to reproductive ageth group, after 20th  gestation age completed, who came for ANC checkup or admitted for hypertension management or prior to delivery in Nepalgunj medical college and teaching hospital under department of Obstetrics and Gynecology from 15th  June to 15th  December 2018. Blood pressure was measured for each women at booking or at subsequent visits. Urine test for protein albumin was performed if blood pressure was elevated. Data was entered and analysis was completed using SPSS package version 20. Results: The overall prevalence of hypertensive disorder during pregnancy was 2.85% among all deliveries. Pre-eclampsia accounted 36% of the cases followed by eclampsia 18%. Gestational hypertension was 21%, pregnancy induced hypertension was 20% and chronic hypertension was only 5% in study population. Conclusion: Knowledge and attitude regarding lack of exercise, low salt diet, obesity and lack of rest were poor contributory risk factors. It is recommended that all pregnant women must develop knowledge about associated risk factors and ensure the application of behavioral change practices to control and prevent hypertensive disorder during pregnancy.

Does genetically-defined ancestry predict booking blood pressure in pregnant women with chronic hypertension?

2019 ◽  
Vol 17 ◽  
pp. S11
Author(s):  
Frances Conti-Ramsden ◽  
Raquel Iniesta ◽  
Phil Chowienczyk ◽  
Lucy C. Chappell
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Chronic Hypertension

Effects of lipoproteins from pre-eclamptic women on umbilical endothelial cell 6-oxo-prostaglandin F1α and endothelin 1 synthesis, and nitric oxide synthase 3 mRNA expression

1999 ◽  
Vol 97 (6) ◽  
pp. 697-706 ◽  
Author(s):  
A. BARDEN ◽  
L. J. BEILIN ◽  
K. BOTH ◽  
J. RITCHIE ◽  
P. LEEDMAN ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cell ◽  
Nitric Oxide Synthase ◽  
Pregnant Women ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Endothelin 1 ◽  
Nitric Oxide Synthase 3 ◽  
Oxide Synthase

In order to evaluate whether lipid abnormalities may contribute to endothelial dysfunction in pre-eclampsia, the present study examined the in vitro effects of very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL), isolated from women with pre-eclampsia and matched controls, on the endothelial synthesis of 6-oxo-prostaglandin F1α (6-oxo-PGF1α; a metabolite of prostacyclin) and endothelin 1, and on the expression of nitric oxide synthase 3 (NOS3) mRNA. VLDL, LDL and HDL cholesterol were isolated from 20 pre-eclamptic and 20 age- and gestation-matched normal pregnant women. The lipoproteins (50 μg/ml) and lipoprotein-free control plasma were incubated for 1, 3 and 6 h at 37 °C with a human umbilical endothelial cell line. The synthesis of 6-oxo-PGF1α and endothelin 1, and NOS3 mRNA expression, were measured at each time point. VLDL from pre-eclamptic women stimulated endothelial cell 6-oxo-PGF1α synthesis to a lesser extent than that from normal pregnant women (P< 0.05). LDL from women with pre-eclampsia also stimulated 6-oxo-PGF1α synthesis to a lesser extent than LDL from normal pregnant women, but the effect was less sustained. The effect of HDL from women with pre-eclampsia on 6-oxo-PGF1α synthesis was similar to that of HDL from normal pregnant women. The pre-incubation levels of lipid peroxides in VLDL and LDL were not different between the normal pregnant and pre-eclamptic women, and cannot account for the decrease in 6-oxo-PGF1α synthesis. VLDL, LDL and HDL from women with pre-eclampsia did not affect endothelial cell synthesis of endothelin 1 or expression of NOS3 mRNA differently from lipoproteins from normal pregnant women. This study suggests that VLDL, and to a lesser extent LDL, from women with pre-eclampsia could potentially contribute to the reduced systemic 6-oxo-PGF1α synthesis observed in the pre-eclamptic syndrome.

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