scholarly journals Arylesterase activity of paraoxonase 1 and intensity of oxidative processes in the blood of dialysis patients

2019 ◽  
pp. 30-38
Author(s):  
L. Korol ◽  
N. Stepanova ◽  
V. Vasylchenko
Keyword(s):  
Reference Group ◽  
Paraoxonase 1 ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Stress Markers ◽  
Total Peroxidase Activity ◽  
Oxidative Processes ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

This study aimed to investigate the arylesterase activity of paraoxonase 1 (PON-1) in patients with end-stage renal disease (ESRD) and its relationship with oxidative stress markers. Methods. We conducted a one-time prospective observational study involving 58 ESRD patients. Among them, there were 20 hemodialyses (HD) patients and 38 patients treated with peritoneal dialysis (PD). The activity of PON-1 in serum was determined spectrophotometrically by the number of phenolic complexes formed using phenylacetate. Besides, spectrophotometrically the concentrations of malondialdehyde, serum concentrations of ceruloplasmin, thiol groups and total peroxidase activity (TPA) of erythrocyte were determined. The reference group consisted of 30 conditionally healthy individuals. Results. The arylesterase activity of the PON-1 in reference group was 6.57 kU/L versus 2.25 kU/L in HD patients and 4.26 kU/L in PD patients (p ˂ 0.0001). A direct correlation was found between arylesterase activity of PON-1 and ceruloplasmin concentration (p = 0.004), and TPA (p = 0.02) in HD patients. The activity of PON-1 in the serum of PD patients was associated with high-density lipoproteins (p ˂ 0.0001). Conclusions. We observed a decrease in the arylesterase activity of PON-1 in ESRD patients compared to the control group. The lowest activity of PON-1 is determined in HD patients. Moreover, the association of the PON-1 activity with a decrease in antioxidant blood markers was found. The enzyme activity in PD patients correlated with increased blood HDL. Further studies involving a larger dialysis cohort of patients are needed to determine the pathogenetic role of PON-1 activity in the development of cardiovascular events in ESRD patients.

scholarly journals P1591SERUM PARAOXONASE 1 ARYLESTERASE ACTIVITY AS AN ANTIOXIDANT MARKER IN DIALYSIS PATIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Lesya Korol ◽  
Natalia Stepanova ◽  
Victoria Vasylchenko
Keyword(s):  
Oxidative Stress ◽  
Reference Group ◽  
Kidney Diseases ◽  
Group Versus ◽  
High Density ◽  
Paraoxonase 1 ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Dialysis Patients ◽  
Esrd Patients

Abstract Background and Aims Patients with chronic kidney disease (CKD) have the highest risk for cardiovascular disease associated with high-density lipoprotein deficiency, chronic inflammation and the development of oxidative stress (OS). This study aimed to investigate serum paraoxonase 1 (PON-1) arylesterase activity in patients with end-stage renal disease (ESRD) and its association with oxidative stress markers. Method We conducted a cross-sectional observational study involving 58 ESRD patients. Among them, there were 20 hemodialyses (HD) patients and 38 patients treated with peritoneal dialysis (PD). Serum PON1 arylesterase activity was determined spectrophotometrically by the number of formed phenolic complexes using phenylacetate. Also, the concentrations of malondialdehyde, serum concentrations of ceruloplasmin, thiol groups and total peroxidase activity (TPA) in erythrocyte were determined spectrophotometrically. In addition, the blood lipid spectrum (including high-density lipoproteins) was determined in all patients. The reference group consisted of 30 conditionally healthy individuals. For the statistical analysis, we used Kruskal-Wallis’s t-test and Spearman's rank correlation test. All statistical analyses were performed using MedCalc. Results Serum PON1 arylesterase activity was 6.57 kU/L in reference group versus 2.25 kU/L in HD patients and 4.26 kU/L in PD patients (p < 0.0001) (Fig. 1). A direct association was found between serum PON1 arylesterase activity and ceruloplasmin concentration (r = 0.38; p = 0.004) and TPA (r = 0.32; p = 0.02) in HD patients. In iddition, serum PON1 arylesterase activity was associated with high-density lipoproteins (r = 0.67; p < 0.0001) in PD patients (Fig. 2). Conclusion We observed a decrease in serumPON1 arylesterase activityin ESRD patients compared to the control group. The lowest level of serum PON1 arylesterase activity was determined in HD patients. Moreover, the association between serum PON1 arylesterase activity and a decrease in antioxidant blood markers was found. Our results support the hypothesis that OS is an important mediator in the progression of kidney diseases and indicates a potential antioxidant role of serum PON1 arylesterase activity in ESRD patients. We suggest using serum PON1 arylesterase activity as an antioxidant marker in dialysis patients.

scholarly journals Diffusion Tensor Imaging Features of Watershed Segmentation Algorithm for Analysis of the Relationship between Depression and Brain Nerve Function of Patients with End-Stage Renal Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Feng Zhu ◽  
Jiao Xu ◽  
Mei Yang ◽  
Haitao Chi
Keyword(s):  
End Stage Renal Disease ◽  
Diffusion Tensor ◽  
Segmentation Algorithm ◽  
Control Group ◽  
Nerve Function ◽  
Watershed Segmentation ◽  
Stage Renal Disease ◽  
End Stage ◽  
The Relationship ◽  
Esrd Patients

The aim of this research was to explore the relationship between depression and brain nerve function in patients with end-stage renal disease (ESRD) and long-term maintenance hemodialysis (MHD) based on watershed segmentation algorithm using diffusion tensor imaging (DTI) technology. A total of 29 ESRD patients with depression who received MHD treatment in the hemodialysis center of hospital were included as the research subjects (case group). A total of 29 healthy volunteers were recruited as the control group, and a total of 29 ESRD patients with depression and brain lesions were recruited as the control group (HC group). Within 24 h after hemodialysis, the blood biochemical indexes were collected before this DTI examination. All participants completed the neuropsychological scale (MoCA, TMT A, DST, SAS, and SDS) test. The original DTI data of all subjects were collected and processed based on watershed segmentation algorithm, and the results of automatic segmentation according to the image were evaluated as DSC = 0.9446, MPA = 0.9352, and IOU = 0.8911. Finally, the average value of imaging brain neuropathy in patients with depression in the department of nephrology was obtained. The differences in neuropsychological scale scores (PSQI, MoCA, TMTA, DST, SAS, and SDS) between the two groups were statistically significant ( P < 0.05 ). The differences of FA values in all the white matter partitions of Fu organs, except the cingulum of hippocampus (CgH) between the two groups, were statistically significant ( P < 0.05 ). ESRD and DTI quantitative detection under the guidance of watershed segmentation algorithm in MHD patients showed that ESRD patients can be early identified, so as to carry out psychological nursing as soon as possible to reduce the occurrence of depression, and then protect the brain nerve to reduce brain neuropathy.

scholarly journals Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Violeta Dopsaj ◽  
Aleksandra Topić ◽  
Miljan Savković ◽  
Neda Milinković ◽  
Ivana Novaković ◽  
...  
Keyword(s):  
Renal Disease ◽  
Diagnostic Accuracy ◽  
End Stage Renal Disease ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Deficiency Anemia ◽  
Control Group ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 (<0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin ≥9.32 ng/mL, ferritin ≥48.2 μg/L, transferrin saturation ≥16.8%, and MCV ≥81 fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.

scholarly journals Cytomegalovirus (CMV) and C-Reactive Protein (CRP) and their Association with Atherosclerosis in Patients with End Stage Renal Disease

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
A M Okba ◽  
M A Raafat ◽  
M N Farres ◽  
N A Melek ◽  
M M Doss ◽  
...  
Keyword(s):  
Mean Value ◽  
Control Group ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
The Mean ◽  
Stage Renal Disease ◽  
End Stage ◽  
Serum Crp ◽  
Atherosclerotic Changes ◽  
Esrd Patients

Abstract Background ESRD (end stage renal disease) is associated with an increase in the risk for cardiovascular disease, which can only be partially explained by known classical risk factors. However, chronic inflammation and endothelial dysfunction are key events in the development of atherosclerosis; both are observed in ESRD patients . The significance of C-Reactive Protein (CRP) and inflammation has increased over time, especially in the ESRD population. From being a simple marker, it now shown that CRP has an active participation in pro-atherosclerotic phenomenon including local pro-inflammatory and thrombotic events. Studies in the general population indicate the usefulness of CRP in prognosis and in monitoring response to therapy. Cytomegalovirus (CMV) is an important pathogen in immunocompromised individuals. Patients with ESRD display signs of frequent CMV re-activation, which may be caused by the uraemia-associated defect in cellular immunity. It has been well documented that hemodialysis patients have impaired immune response, which may result in higher prevalence rates of viral infections, including CMV. Infections in these patients may be due to primary infection or, more commonly, by reactivation of latent virus or re-infection with exogenous virus, which may be introduced by blood transfusion or kidney transplant. Infection with CMV is also considered a risk factor for progression of atherosclerotic disease. Methods CRP and CMV IgG level was measured in the blood samples of sixty adult patients diagnosed as ESRD, 30 ESRD patients with atherosclerotic changes(Group I) and 30 ESRD patients without atherosclerotic changes (Group II) and in comparison with 30 control subjects(Group III) (Control Group). Results The mean value of CRP in the control group (6.0 ± 4.2), the mean value in the ESRD patients with atherosclerotic changes group (15.8 ± 5.6) and the mean value in the ESRD patients without atherosclerotic changes group (11.2 ± 3.9),thus the mean values of CRP in ESRD patients groups were significantly higher than that of the control group (P &lt; 0.001) and the mean value of CRP in ESRD with atherosclerotic changes is significantly higher compared to ESRD without atherosclerotic changes group (P &lt; 0.001). Regarding CMV IgG antibodies it was significantly higher in ESRD patients compared to the control group and was also significantly higher in ESRD with atherosclerotic changes compared to ESRD without atherosclerotic changes. Conclusions ESRD are at greater risk of inflammatory reaction against factors originating from graft, fistula, dialysis membrane, infection sites. These reactions are associated with increased levels inflammatory markers such as serum CRP. Serum CRP seems to have a contribution in the development of cardiovascular complications in ESRD patients.CMV seropositivity is also significantly associated with atherosclerotic disease in ESRD patients. Our data suggest that the risk for progressive atherosclerosis is specifically increased in patients with an inflammatory response to CMV and elevated CRP level.

Bifidobacteriaceae family diversity in gut microbiota of patients with renal failure

2020 ◽  
Author(s):  
Gholamreza Hanifi ◽  
Hamid Tayebi Khosroshahi ◽  
Reza Shapouri ◽  
Mohammad Asgharzadeh ◽  
Hossein Samadi Kafil
Keyword(s):  
Gut Microbiota ◽  
Control Group ◽  
Disease Group ◽  
Bifidobacterium Adolescentis ◽  
Fecal Samples ◽  
Health Promoting ◽  
Stage Renal Disease ◽  
End Stage ◽  
And Control ◽  
Esrd Patients

Abstract Background: Bifidobacteriaceae family are belonged to the gut microbiota that could exhibit probiotic or health promoting effects on the host. Several studies suggested that gut microbiota are quantitative and qualitative altered in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). The present study was aimed to assess the members of Bifidobacteriaceae family in fecal samples of patients with CKD and ESRD in compared to non-CKD/ESRD patients to find any changes of their counts in these patients.Methods: Twenty fresh fecal samples of patients with CKD/ESRD and twenty from non-CKD/ESRD patients were included. The whole DNA of fecal samples were extracted and the gut microbiota composition was analyzed by next generation sequencing (NGS) method.Results: Total 651 strains were identified from 40 fecal samples, which 8 (1.23%) strains were identified as family Bifidobacteriaceae. The most abundance species in both control and disease group were Bifidobacterium adolescentis (2.10% ± 1.05% vs. 1.98% ± 1.53%, respectively) and the lowest abundance species in disease group was Bifidobacterium animalis subsp. lactis (0.0007% ± 0.0009%).Conclusions: There was no significant differentiation in the abundance of various species between disease group and control group (p<0.05). This study has confirmed that the members of Bifidobacteriaceae family are not alters in patients with CKD/ESRD.

scholarly journals Cell-free DNA as a marker for the outcome of end-stage renal disease patients on haemodialysis

2020 ◽  
Author(s):  
Susana Coimbra ◽  
Susana Rocha ◽  
Henrique Nascimento ◽  
Maria João Valente ◽  
Cristina Catarino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
End Stage Renal Disease ◽  
Stress Markers ◽  
Free Dna ◽  
All Cause Mortality ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients ◽  
And Oxidative Stress

Abstract Background DNA damage and inflammation are common in end-stage renal disease (ESRD). Our aim was to evaluate the levels of circulating cell-free DNA (cfDNA) and the relationship with inflammation, anaemia, oxidative stress and haemostatic disturbances in ESRD patients on dialysis. By performing a 1-year follow-up study, we also aimed to evaluate the predictive value of cfDNA for the outcome of ESRD patients. Methods A total of 289 ESRD patients on dialysis were enrolled in the study: we evaluated cfDNA, haemogram, serum iron, hepcidin, inflammatory and oxidative stress markers, and haemostasis. Events and causes of deaths were recorded throughout the follow-up period. Results ESRD patients, as compared with controls, presented significantly higher levels of cfDNA, hepcidin, and inflammatory and oxidative stress markers, and significantly lower values of iron and anaemia-related haemogram parameters. The all-cause mortality rate was 9.7%; compared with alive patients, deceased patients (n = 28) were older and presented significantly higher values of inflammatory markers and of cfDNA, which was almost 2-fold higher. Furthermore, cfDNA was the best predictor of all-cause mortality and cardiovascular mortality in ESRD patients, in both unadjusted and adjusted models for basic confounding factors in dialysis. Conclusions Our data show cfDNA to be a valuable predictive marker of prognosis in ESRD patients on dialysis treatment; high levels of cfDNA were associated with a poor outcome.

scholarly journals Effects of Peer Support Program on Self-Management in Patients with End-Stage Renal Disease Undergoing Hemodialysis

2020 ◽  
Vol 10 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Fida' Husain ◽  
Henni Kusuma ◽  
Andrew Johan
Keyword(s):  
Peer Support ◽  
End Stage Renal Disease ◽  
Intervention Group ◽  
Support Programs ◽  
Self Management ◽  
Control Group ◽  
The Mean ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

Background: End-stage renal disease (ESRD) patients undergoing hemodialysis require essential self-management to lifestyle changes to minimize the risk of complications, morbidity, and mortality. Efforts made to improve self-management of hemodialysis patients in previous studies were carried out by health workers that may not provide 'real' knowledge, while peer support programs carried out by patients as peers to share their experiences may provide more benefits.Purpose: The purpose of this study was to determine the effects of peer support programs on improving self-management in patients with ESRD undergoing hemodialysis.Methods: This study employed a quasi-experimental design and involved a total of 33 patients in the control group and 32 patients in the intervention group, who met the inclusion and exclusion criteria. The samples were recruited consecutively. The intervention of peer support programs was implemented through information support, emotional support, and mutual reciprocity in groups of 10-12 people to share experiences related to their self-management. The intervention was given for six sessions; each lasted for 30-45 minutes. The data were collected using the Indonesian version of the hemodialysis self-management instrument (HDSMI) and analyzed using a paired-sample t-test and independent-sample t-test.Results: The results showed that after the intervention, the mean score of self-management in the intervention group increased from 79.47±7.919 to 90.75±7.089, and in the control group, the mean increased from 81.88±8.291 to 82.12±7.692. After the implementation of peer support programs, there was a significant difference in the score of self-management between the intervention and control groups (p<0.001).Conclusion: Peer support programs gave an effect on increasing self-management in patients with ESRD undergoing hemodialysis. Peer support programs should be introduced early to ESRD patients undergoing hemodialysis so that they can learn about self-management from other patients.  

Impaired resistance artery function in patients with end-stage renal disease

2011 ◽  
Vol 120 (12) ◽  
pp. 525-536 ◽  
Author(s):  
Natallia Luksha ◽  
Leanid Luksha ◽  
Juan Jesús Carrero ◽  
Folke Hammarqvist ◽  
Peter Stenvinkel ◽  
...  
Keyword(s):  
Renal Disease ◽  
End Stage Renal Disease ◽  
Nitrosative Stress ◽  
Control Group ◽  
Strong Impact ◽  
Arterial Distensibility ◽  
Functional Features ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

We investigated an effect of uraemia on structural and functional features of human resistance vasculature. Arteries (≈ 200 μm) isolated from subcutaneous fat biopsies obtained from 35 ESRD (end-stage renal disease) patients starting peritoneal dialysis and 30 matched controls were studied using isolated small artery bioassays. Flow-mediated dilatation was attenuated in ESRD patients compared with controls. NO (nitric oxide) contribution to flow was lacking in ESRD patients, but present in the controls. ADMA (asymmetrical dimethyl L-arginine) levels were higher in the ESRD group compared with the control group. Dilatation in response to acetylcholine was reduced in ESRD patients compared with controls, but response to NO donor was similar. Expression of nitrotyrosine and heat shock proteins 70 and 27, but not 90, was increased in arteries from ESRD patients compared with controls. Arterial remodelling was absent in ESRD patients. There was no difference between the groups in myogenic tone, vascular reactivity or sensitivity to several vasoconstrictors. Arterial distensibility, reflecting passive properties of the vascular wall, was reduced in ESRD patients compared with controls. Exclusion of ESRD patients with diabetes and/or cardiovascular disease from analyses had no influence on the main findings. Thus we propose that uraemia has a strong impact on endothelial function and passive properties of the arterial wall of human peripheral resistance vasculature. The reduced contribution of NO to flow stimulus via enhanced nitrosative stress and higher plasma concentrations of ADMA may suggest potential mechanisms behind endothelial dysfunction in the resistance peripheral circulation in ESRD.

Exploring the Association between Changes in Dialysis Reimbursement Policies and Care Outcomes of Peritoneal Dialysis

2020 ◽  
Author(s):  
Ray-E Chang ◽  
Shih-Pi Lin ◽  
Feng-Jung Yang ◽  
Robert C. Myrtle
Keyword(s):  
Peritoneal Dialysis ◽  
Cost Effective ◽  
Control Group ◽  
Technical Failure ◽  
Care Providers ◽  
Before And After ◽  
Stage Renal Disease ◽  
End Stage ◽  
The Impact ◽  
Esrd Patients

Abstract Background: Except for renal transplantation, peritoneal dialysis (PD) is considered to be relatively cost-effective option for end-stage renal disease (ESRD) patients. Less than 7% of ESRD patients receiving PD in Taiwan, and the promising benefits of PD treatment influenced health policy makers to seek ways to encourage PD utilization. The purpose of this study is to evaluate the effect of their policy initiatives.Methods: An observational longitudinal study using a before-and-after analysis was conducted. The propensity score matching technique was employed to match PD patients before and after the introduction of Taiwan’s efforts to encourage PD utilization in ESRD patients, and the change in PD technical failure was analyzed. HD patients were also matched as the control group to assess the impact of Taiwan’s PD utilization encouraging policies on mortality in PD patients. The competing risk regression approach for survival analysis was adopted in our study.Results: The results showed that while the PD encouraging policies had increased the PD utilization, the increase in PD utilization was accompanied by an increase in technique failure and an increase in mortality.Conclusions: The adoption of new treatments which may benefit patients and incentives to change physician practice behaviors require more disciplined and carefully managed implementation efforts. Care providers need to be equipped by adequate training and sufficient manpower as part of the policy package.

Differential Modulation of Cytokines by VEGF In End Stage Renal Disease

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 5187-5187
Author(s):  
Vinod Bansal ◽  
Debra Hoppensteadt ◽  
Evangelos Litinas ◽  
Indermohan Thethi ◽  
Jawed Fareed
Keyword(s):  
Inflammatory Mediators ◽  
Inflammatory Responses ◽  
Conflicts Of Interest ◽  
Maintenance Hemodialysis ◽  
Control Group ◽  
Cellular Activation ◽  
Stage Renal Disease ◽  
End Stage ◽  
Endothelial Growth Factor ◽  
Esrd Patients

Abstract Abstract 5187 Vascular endothelial growth factor (VEGF) mediates nitric oxide dependent angiogenic effects contribute to the inflammatory responses. VEGF is upregulated in ESRD and contributes to the cardiovascular dysfunction in ESRD. VEGF is also upregulated by erythropoietin which is commonly used in the management of ESRD patients. With the availability of BioChip Array Technology, a multiparametric screening of inflammatory mediators/cytokines can be measured and relevance of VEGF can be established. Utilizing the Randox Biochip technology, we profiled the plasma samples from 53 ESRD patients to determine their relevance to VEGF levels. Pre-dialysis samples from 53 male and female ESRD patients on maintenance hemodialysis were analyzed using a cytokine biochip for VEGF, IL2, IL4, IL6, IL8, IFNG1, TNF, IL1, MCP-1 and EGF. In addition, NO, asymmetric dimethyl arginine (ADMA), serotonin and functional microparticles levels were also measured. A group of 50 normal healthy males and females constituted the control group. The circulating levels of VEFG and mediators were compared with the normals. In addition, VEGF levels were compared with the other mediators. In comparison to the normals, ESRD patients exhibited decreased levels of IL4, IL8, TNF and EGF, which ranged from 20–210%. Increased levels of IL2, IL6, IL10, IFNG1, IL1 and MCP-1 were noted (20-350%). NO levels were also increased, however ADMA remained unchanged. Both the serotonin and microparticle levels were also elevated in comparison to the normals (p>0.05) VEGF levels showed wide variation, however on a cumulative basis a 30–210% increase in VEGF was noted. Except for the MCP-1, the correlation of VEGF with other inflammatory markers was relatively poor (r2=<0.25). These results show that ESRD patients can be profiled for various inflammatory mediators. VEGF levels were markedly increased, however only MCP-1 levels correlated well with the increase in VEGF levels. Additional studies are warranted to clarify the regulatory role of VEGF and other mediators on inflammatory cytokines and cellular activation in ESRD. Disclosures: No relevant conflicts of interest to declare.

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