Karakteristik dan kualitas semen sapi Sumba Ongole dalam pengencer BTS yang dimodifikasi dengan susu kedelai

This study aimed to find out the quality of fresh semen from Sumba Ongole (SO) bulls which was diluted with Bestvile Thawing Solution (BTS) diluent modified with soybean milk (SKD). The study used a completely randomized design consisting of four treatments and 5 replications, P0 (100 % BTS), P1 (95 % BTS + 5 % SM), P2 (90 % BTS + 10 % SM), and P3 (85 % BTS + 15 % SM). The parameters observed were the colour, smell, consistency, pH, volume, motility, viability, and abnormalities of spermatozoa. The results showed that the characteristics were in the normal, creamy white semen colour, semen volume 3,6 ml, medium consistency, pH 6,3, typical SO bulls smell, sperm motility 85 %, mass activity +++, sperm concentration 1276 million/ml, live sperm 90,05 % and sperm abnormalities 6,15 % and quality there was no significant difference between the four treatments. The spermatozoa motility and viability in each treatment was able to which achieved on day 1. The characteristics of the spermatozoa of SO bulls were in the normal category. Meanwhile, BTS diluent with the addition of SKD was unable to maintain sperm quality.

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Kaji Banding Kualitas Semen Segar Empat Genetik Ayam Lokal Indonesia (COMPARATIVE STUDY ON THE QUALITY OF FRESH SEMEN OF FOUR GENETIC LOCAL CHICKEN IN INDONESIA)

jurnal veteriner ◽

10.19087/jveteriner.2019.20.3.397 ◽

2019 ◽

Vol 20 (3) ◽

pp. 397

Author(s):

Junaedi Junaedi ◽

Husnaeni Husnaeni

Keyword(s):

Comparative Study ◽

Spermatozoa Motility ◽

Genetic Groups ◽

Randomized Design ◽

Semen Volume ◽

Completely Randomized Design ◽

Local Chicken ◽

Fresh Semen ◽

Better Than

This study was conducted to examine the comparison the quality of spermatozoa in four genetic groups of poultry (Pelung chicken, Nunukan chicken, Sentul chicken, and Bangkok chicken). The parameters of this study were the concentration of spermatozoa, motility, viability, semen volume, color, and consistency. The study was designed using a Completely Randomized Design (CRD) with four local chicken nations and four replications. The results showed that the volume of Nunukan chicken semen was 0.32±0.01 mL/ejaculation, Bangkok chicken was 0.31±0.01 mL/ejaculate, Sentul chicken was 0.15±0.02 mL/ejaculate and Pelung chicken was 0.23±0.02 mL/ejaculation. the color of fresh semen is white (Pelung chicken, Nunukan chicken and Sentul chicken) except chicken Bangkok has the color of cream semen. Pelung chicken has a concentration of spermatozoa 5,043.33±51 million/mL, Nunukan chicken 3,250.22 ±45 million/mL, Sentul chicken 3,002.87±67 million/mL, and Bangkok chicken 3,002.87 ± 67 million/mL. Motility of Pelung chicken 84.,69 ± 1.12%, Bangkok chicken 82.35±1.85%, Nunukan chicken 77.74±1.57% and Sentul chicken 77.64±1.65%. Viability spermatozoa of Sentul chicken was 90.35 ± 1.21%, Bangkok chicken was 90.64 ± 1.16%, Pelung chicken was 89.17 ± 1.23% and Nunukan chicken was 86.29 ± 1.15%. It can be concluded that the motility spermatozoa of Pelung chicken and Bangkok chicken is better than the spermatozoa motility of Nunukan chicken and Sentul chicken. The viability of Sentul chicken and Bangkok chicken was higher compared to the viability semen of chicken Pelung and chicken Nunukan.

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Effect of Storage Time of Epididymis at 5 oC on Spermatozoa Quality of Aceh Local Goat

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v4i2.9811 ◽

2010 ◽

Vol 4 (2) ◽

Author(s):

Hamdan Hamdan ◽

Budianto Panjaitan ◽

Amalia Sutriana ◽

Dwinna Aliza ◽

Erdiansyah Rahmi

Keyword(s):

Experimental Design ◽

Storage Time ◽

Sperm Concentration ◽

Spermatozoa Motility ◽

Randomized Design ◽

Sperm Abnormality ◽

Significant Difference ◽

Completely Randomized Design ◽

Banda Aceh

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.

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Kualitas spermatozoa semen sexing kambing peranakan etawa (pe) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda

Jurnal Sains Peternakan ◽

10.21067/jsp.v5i1.3136 ◽

2017 ◽

Vol 5 (1) ◽

pp. 39-49

Author(s):

Kornelis Tamo Ama ◽

Enike Dwi Kusumawati ◽

Aju Tjatur Nugroho Krisnaningsih

Keyword(s):

Semen Quality ◽

Sperm Quality ◽

Egg White ◽

Laboratory Research ◽

Sedimentation Method ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Fresh Semen

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui kualitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing PE. Metode percobaan yang digunakan adalah percobaan laboratorium dengan menggunakan rancangan acak lengkap (RAL) Setiap perlakuan semen sexing kambing Peranakan Etawa dengan pengencer yang berbeda yaitu (P0) CEP, (P1) CEP+kuning telur+putih telur, (P2)CEP+putih telur (P3), CEP+kuning telur dan diulang sebanyak 10 kali. Variabel yang diukur pada penelitian ini adalah motilitas, viabilitas, dan abnormalitas spermatozoa. Data yang diperoleh dianalisis dengan mengunakan analisis varian. Apabila perlakuan memberikan pengaruh maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa kualitas spermatozoa kambing Peranakan Etawa (PE) dengan berbagai macam pengencer menunjukkan pengaruh yang sangat nyata (P<0,01). Motilitas dan Viabilitas menunjukkan perbedaan yang sangat nyata (P<0,01) pada berbagai pengencer Motilitas terbaik pada beberapa lapisan atas sebesar 65% dan CEP+PT lapisan atas 60% dilanjutkan oleh CEP+KT+PT lapisan bawah 55,9% dan CEP+PT lapisan bawah 55% dilanjutkan lagi oleh CEP+KT lapisan atas 50% dan lapisan bawah 45% dan yang paling terkecil adalah CEP lapisan bawah 40%. Viabilitas spermatozoa dari yang tertinggi yaitu CEP+KT+PT lapisan atas sebesar 69,553% dan CEP+PT lapisan atas sebesar 69,519% dan dilanjutkan dengan CEP+PT lapisan bawah sebesar 65,504% dan CEP+KT+PT lapisan bawah 65,473%, dan dilanjutkan lagi dengan CEP+KT lapisan atas sebesar 60,269% dan lapisan bawah sebesar 53,476% dan yang paling terkecil yaitu CEP lapisan bawah sebesar 40,371%. Presentase Abnormalitas menunjukkan bahwa perlakuan tidak memberikan pengaruh nyata (P>0,05). Tetapi CEP+KT+PT lapisan atas menunjukkan persentase yang paling rendah yaitu sebesar 4,88% dibandingkan perlakuan lainnya. Dari hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer CEP+ kuning telur + putih telur pada lapisan atas memberikan hasil terbaik terhadap kualitas spermatozoa ditinjau dari motilitas, viabilitas dan abnormalitas. ABSTRACT The purpose of this study was to determine the sexing sperm quality of Etawa cross-bred goat (PE) with egg white sedimentation method using different diluents. The material used in this study was the fresh semen Etawa crossbreed goat (PE) of the center for Artificial Insemination (BBIB) Singosari Malang. The method is laboratory research using completely randomized design. Consist of CEP, CEP + KT, CEP + PT, CEP + KT + PT and repeated 10 times. The variables are motility, viability and abnormality of sperm. Data were analyzed by using variance analysis. If the treatment effect then continued by Least Significant Difference (LSD). The results showed that the quality of Etawa cross-bred goat sperm with various diluents showed a significant influence (P <0.01). Motility and viability showed differences (P <0.01) in various diluents. The best motility and viability on top layer of CEP+KT+PT diluent were 65% and 69%, 55%. Percentage abnormalities showed that the treatment was not significant effect (P> 0.05). but top layer CEP+KT+PT diluent shows the percentage of abnormality west 4.88% compared to the other treatments. From the results of this study concluded that the quality of sexing semen quality by using dilution CEP + yolk white egg on the top layer gives the best results on the quality of sperm in terms of motility, viability and abnormalities. Based on this study it is suggested that the use of sexing semen with egg white sedimentation method using diluent CEP + yolk + white egg.

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Effect of different extenders on quality of frozen-thawed boar semen

Indian Journal of Animal Research ◽

10.18805/ijar.7050 ◽

2015 ◽

Vol 49 (6) ◽

Cited By ~ 3

Author(s):

S Frydrychová ◽

A Lustyková ◽

E Václavková ◽

J Lipenský ◽

M Rozkot

Keyword(s):

Sperm Motility ◽

Aspartate Aminotransferase ◽

Sperm Concentration ◽

Holding Time ◽

Holding Period ◽

Semen Volume ◽

Significant Difference ◽

Boar Semen ◽

Fresh Semen

The objective of this study was to investigate the effect of using different extenders <italic>viz.</italic> Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

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Pengaruh Lama Thawing yang Berbeda pada Suhu 25 oC Terhadap Kualitas Semen Beku Sapi Ongole

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.6538 ◽

2019 ◽

Vol 6 (1) ◽

pp. 119

Author(s):

Enike Dwi Kusumawati ◽

Syam Rahadi ◽

Sugeng Santoso ◽

Dyah Lestari Yulianti

Keyword(s):

Sperm Quality ◽

Animal Husbandry ◽

Randomized Design ◽

Frozen Semen ◽

Significant Difference ◽

Completely Randomized Design ◽

Bull Sperm

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh lama thawing yang berbeda pada suhu 250C untuk mendapatkan kualitas spermatozoa semen beku sapi ongole yang optimal. Penelitian dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang. Metode Penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Setiap perlakuan thawing diberikan ulangan sebanyak 10 sampel semen beku sapi ongole. Hasil penelitian menunjukkan bahwa perlakuan dengan lama thawing 7, 15, 30 detik pada suhu 25oC memberikan perbedaan pengaruh yang sangat nyata (p<0.01) pada motilitas, viabilitas dan abnormalitas spermatozoa semen beku sapi ongole. Motilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 40,8%, viabilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 82,39%, sedangkan abnormalitas terendah diperoleh pada perlakuan P3 dengan rata-rata sebesar 11,95%. Berdasarkan penelitian ini maka dapat disimpulkan bahwa thawing pada suhu 25oC dengan lama waktu 30 detik memberikan kualitas spermatozoa yang paling baik sehingga disarankan untuk melakukan thawing pada suhu 25oC dengan lama waktu 30 detik. Kata kunci: abnormalias, mortalitas, ongole, thawing, viabilitas ABSTRACT This study aims to determine the effect of different thawing time at 250C to get the sperm quality of frozen semen ongole bull. Research conducted at the Laboratory of Animal Husbandry Faculty Kanjuruhan University of Malang. The method of study by using Completely Randomized Design (CRD) factorial. Any treatment given repeated thawing of frozen samples of 10 times. The study show that treatment with time thawing 7 (P1), 15 (P2), 30 (P3) seconds at 25oC gives a very significant difference (P<0.01) on motility, viability and abnormalities of ongole Bull sperm. The highest motility and viability were obtained at P3 (40,8%) and (82,39%), while the lowest abnormalities obtained on P3 (11,95%). Based on this research it can be concluded that thawing at 25oC with 30 seconds to give the best quality sperm that is recommended for thawing. Keywords: abnormality, motility, ongole, thawing, viabilit

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Cryopreservation and quality assessment of boar semen collected from bulk samples

Veterinární Medicína ◽

10.17221/125/2018-vetmed ◽

2019 ◽

Vol 64 (No. 5) ◽

pp. 209-216 ◽

Cited By ~ 1

Author(s):

R Ratchamak ◽

T Vongpralub ◽

W Boonkum ◽

V Chankitisakul

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Sperm Quality ◽

Sperm Concentration ◽

Bulk Sample ◽

Energy Status ◽

Semen Volume ◽

Boar Semen ◽

Live Sperm ◽

Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P < 0.05) had a lower mean sperm concentration (P < 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P < 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

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Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation

Research Society and Development ◽

10.33448/rsd-v10i16.23382 ◽

2021 ◽

Vol 10 (16) ◽

pp. e408101623382

Author(s):

Diego André Costa Saranholi ◽

Rafael Rocha de Paula ◽

Edmilson Pytilak ◽

Fabíola Afonso ◽

Luis Felipe Canela ◽

...

Keyword(s):

Statistical Difference ◽

Sperm Quality ◽

Sperm Concentration ◽

Physical Parameters ◽

Significant Difference ◽

Before And After ◽

Holstein Bulls ◽

The Difference ◽

Fresh Semen

The aim of this study was to evaluate parameters indicative of sperm quality of fresh and post-thawed semen of Aberdeen Angus, Holstein and Nelore bulls. Thirty-nine bulls were used: Aberdeen Angus (n=13), Holstein (n=13) and Nelore (n=13). The ejaculate collects were performed twice a week using artificial vagina, totaling 792 semen collections, 307 for Aberdeen Angus, 225 for Holstein and 260 for Nelore bulls. After collection, fresh semen was evaluated and semen freezing was performed. After freezing, the batches were thawed and progressive motility was determined. The analysis of fresh semen showed that there was no difference (P = 0.053) between the Aberdeen Angus and Nelore breeds, while ejaculates from Holstein bulls showed a statistical difference (P = 0.024). As well, a difference (P<0.001) was identified in the sperm concentration of the three breeds. In the samples evaluated after thawing, a statistical difference was observed between Holstein and Nelore breeds (P<0.001), while the values of the Angus breed were similar to the other two breeds. The difference in motility of fresh and post-thawing semen showed that Nelore and Angus bulls showed greater variation in values between the analyzes (26.0±8.9% and 25.3±8.4%, respectively) showing a significant difference (P<0.001) in relation to Holstein bulls (20.6±9.3%) that obtained the smallest difference. The analysis of fresh and post-thawing semen did not show any significant difference (P=0.13) between breeds. In conclusion, the semen cryopreservation process causes a decrease in the physical parameters of the semen and these quality losses suffer interference according to the breeds.

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PENGARUH PEMBERIAN ZINK (Zn) TERHADAP KUALITAS SPERMATOZOA WISTAR JANTAN DEWASA (Rattus norvegicus) YANG DIBERIKAN MONOSODIUM GLUTAMAT (MSG)

Jurnal e-Biomedik ◽

10.35790/ebm.3.1.2015.6847 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Katarina K. Tandung ◽

Lusiana Satiawati ◽

Benny Wantow

Keyword(s):

Glutamic Acid ◽

Rattus Norvegicus ◽

Sperm Quality ◽

Monosodium Glutamate ◽

Control Group ◽

Spermatozoa Motility ◽

Randomized Design ◽

Study Results ◽

Completely Randomized Design

Abstract: Zinc is a cofactor of more than 100 enzymes that are essential for the metabolism of nucleic acid and protein synthesis. Zinc plays an important role in growth and development, immune response, neurological and reproductive functions. Monosodium glutamate (MSG) is the sodium salt of glutamic acid , a food flavor enhancer in the form of L-glutamic acid. The purpose of this study was to determine the effect of zinc on sperm quality adult male wistar (Rattus norvegicus), following administration of monosodium glutamate (MSG). This study is experimental with a completely randomized design were conducted from November to Desember 2014 using a sample of 6 male wistars as a control group (P0) with 2 mg MSG being administered. The P1 group consist of 2 male wistars with 2 mg of MSG and zinc at a dose of 10 mg as treatment were administered to this group. The last group P2 also consist of 2 male wistars with the administration 2 mg of MSG and 20 mg doses of zinc as treatment.This study results in an increasement of spermatozoa consentration in the treatment group (P2), accounted for 78,25x106 as well as the increased of spermatozoa motility of 43,5 % for the treadment group (P2). Morfologically, 83,5 % of normal spermatozoa were accounted from the same group. Conclusion: Administration of zinc can improve the quality of male wistar spermatozoa following the administration of MSG, which includes concentration, motility and morphology. The increasing dosage of zinc being administered showed higher impact in improving the quality of spermatozoa.Keywords: zinc, MSG, male wistar, the quality of spermatozoa.Abstrak: Zink adalah kofaktor lebih dari 100 enzim yang penting untuk metabolisme asam nukleat dan sintesis protein. Zink berperan penting dalam pertumbuhan dan perkembangan, respon kekebalan, fungsi neurologis dan reproduksi. Monosodium glutamat (MSG) adalah garam natrium dari asam glutamat (glutamic acid), sebagai penambah rasa makanan dalam bentuk L-glutamic acid. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian zink terhadap kualitas spermatozoa wistar jantan dewasa (Rattus norvegicus) yang telah diberi monosodium glutamat (MSG). Penelitian ini bersifat eksperimental dengan rancangan acak lengkap (completely randomized design) yang dilakukan pada bulan November sampai Desember 2014 dengan menggunakan sampel sebanyak 6 ekor terdiri dari 2 ekor wistar pemberian MSG dengan dosis 2 mg sebagai kontrol (P0), 2 wistar pemberian MSG dengan dosis 2 mg dan zink dengan dosis 10 mg sebagai perlakuan (P1), dan 2 wistar pemberian MSG dengan dosis 2 mg dan zink dengan dosis 20 mg sebagai perlakuan (P2). Hasil penelitian ini di dapatkan peningkatan konsentrasi spermatozoa pada kelompok perlakuan P2 sebesar 78,25 x 106, peningkatan motilitas spermatozoa kategori A pada perlakuan kelompok P2 sebesar 43,5% dan morfologi normal spermatozoa pada kelompok perlakuan P2 sebesar 83,5%. Simpulan: Pemberian zink dapat menyebabkan perbaikan pada spermatozoa wistar jantan yang sebelumnya telah di beri MSG meliputi konsentrasi, motilitas, dan morfologi. Peningkatan dosis zink yang diberikan menunjukkan adanya perbaikan kualitas spermatozoa yang semakin meningkat juga.Kata kunci: zink, MSG, wistar jantan, kualitas spermatozoa.

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Evaluasi Kualitas Spermatozoa Ayam Brahma, Cochin dan Bangkok Sebagai Dasar Penerapan Teknologi Inseminasi Buatan pada Ayam Kampung

Jurnal Sains Teknologi & Lingkungan ◽

10.29303/jstl.v0i0.250 ◽

2021 ◽

pp. 1-9

Author(s):

Asnawi Asnawi ◽

Budi Indarsih ◽

Dewi Haryani ◽

Sukartha Jaya ◽

Maya Nachida ◽

...

Keyword(s):

Artificial Insemination ◽

Sperm Quality ◽

Animal Science ◽

Spermatozoa Motility ◽

Animal Reproduction ◽

Randomized Design ◽

Completely Randomized Design ◽

Artificial Vagina ◽

Local Chickens

Research on the evaluation of the spermatozoa quality of Brahma, Cochin and Bangkok chickens as the basis for the application of artificial insemination technology in native chickens has been carried out. The purpose of this study was to evaluate the spermatozoa quality of Brahma, Cochin and Bangkok rooster as the basis for determining the spermatozoa dose for artificial insemination in local chickens. method research was used a completely randomized design (CRD) with three treatments, that is: Brahma, Cochin and Bangkok rooster. Each treatment was repeated five times. Cement storage is carried out by inserting an artificial vagina into the cloaca of the rooster and stimulating it to climb the hen. The collected sperm were analyzed at the Animal Reproduction Laboratory, Faculty of Animal Science, Mataram University. The variables observed included motility, viability and morphology of spermatozoa. The results of studied that the spermatozoa motility of Brahma, Chicin and Bangkok rooster were 72 ± 2.74%. 74 ± 2.24% and 73 ± 2.74% (P> 0.05) respectively; The spermatozoan viability of Brahma rooster and Bangkok rooster was 99.2 ± 1.30%, 99.4 ± 0.55% and 99.2 ± 1.10% (P>0.05)respectively. The spermatozoan morphology of brahma rooster, chicin rooster and Bangkok rooster was 94.6 ± 2.30%, 94.4 ± 2.70% and 97.4 ± 2.07% (P> 0.05), respectively. Cochin rooster and Bangkok rooster respectively, 62.6 ± 7.92 x 107, 57 ± 5.83 x107 , and 65.2 ± 12.28 x107, respectively. The results of the study concluded that Brahma, Cochin and Bangkok rooster had good sperm quality and could be able to used in artificial insemination of Kampung hens.

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KUALITAS MOTILITAS DAN VIABILITAS SPERMATOZOA DARI SEMEN AFKIR SAPI LIMOUSIN PADA PENGENCER SUSU SKIM KUNING TELUR SITRAT DENGAN PENAMBAHAN BERBAGAI KADAR GLUKOSA

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v7i2.2018.96-101 ◽

2020 ◽

Vol 7 (2) ◽

pp. 96

Author(s):

Rudi Irvanto ◽

Hardijanto Hardijanto ◽

Widya Paramita ◽

Suherni Susilowati ◽

Tita Damayanti L ◽

...

Keyword(s):

Research Design ◽

Glucose Level ◽

Skim Milk ◽

Egg Yolk ◽

Spermatozoa Motility ◽

Bull Semen ◽

Randomized Design ◽

Completely Randomized Design

Quality of spermatozoa motility and viability from rejected limousin bull semen diluted with skim milk egg yolk sitrat added with various levels of glucose. Glucose level used were 0%, 0,5%, 1,5%, 2,5%, and 3,5%. Writer was using on four years old Limousine bull. Bull semen used in this research was bull rejected semen with bellow 70% motility. Semen observation was done at 0 hour, 24 hours and 48 hours. Research design used in this study was completely randomized design with faktorial pattern with 5 replicates. Highest result in motility this research was showed at 24 hours with 30% value in glucose 2,5% treatment and 48 hours with 10% value. The lowest result showed in glucose 0% treatment at 24 hours and 0% at 48 hours. Highest result in viability showed on glucose 2,5% treatment with 62,6% value at 24 hours and at 48 hours with 53,4% value. Lowest result in viability showed on glucose 0% treatment with 44,2% value at 24 hours and 31,4% value at 48 hours.

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