scholarly journals Postembryonal ontogenesis of broad whitefish and formed herd under phenol intoxication

Author(s):  
S. A. Seliukova ◽  
М. V. Kibalova ◽  
Iu. A. Usoltseva ◽  
A. G. Seliukov

The paper reveals the data on comparative analysis of broad whitefish Coregonus nasus larvae in the Ob received from natural populations (lower Ob) and from fish, farmed outside their natural habitat. This fish herd was formed from fish brought from the lower Ob basin to the fish farm “Forvat” (Leningrad region), where they were bred during five generations. During a month, the embryos of broad whitefish were incubated in the conditions of Sob fish-breeding plant (Harp village, Yamalo-Nenetsk Autonomous District), then they were placed for breeding into the laboratory of the Institute of Biology of Tyumen State University. The broad whitefish embryos from artificial herd were taken to the laboratory at the stage of eye pigmentation. After hatching, the larvae in both series were found to be chronically intoxicated with phenol 1 (0.001 mg/l), 5 (0.005 mg/l) and 10 (0.01 mg/l) since their transfer to external feeding up to two months of age. The authors observed broad whitefish from artificial herd and found out increased survival rate, higher balance and lower level of hystopathological changes in all variants. Due to low biological parameters of the broad whitefish young stock, their application for stocking natural basins in the conditions of modern anthropogenic impact is not efficient. On the other hand, increased biological and toxicovigilance characteristics are more effective when being applied in the basins with high anthropogenic load with the aim to increase fish productivity.

Author(s):  
M.A. Lukyanov ◽  

A digital government is relevant topic. It is associated with hopes for improving the institutions of public policy and administration. Russia has adopted programs for the development of the digital economy. So that, there is planned the transition to digital government in Russia. The transition to digital government is accompanied by several challenges such as control, centralization, excessive control, etc. An important factor in the success of responding to these challenges is the attitude of citizens to the digitalization of public services. This article presents empirical materials from a sociological study conducted in two regions of the Russian Federation: St. Petersburg and the Leningrad region. The sample size was 300 respondents. The study is the empirical basis for a multistage study conducted by the author as part of the research team of St. Petersburg State University in the period from 2017 to 2020. The article presents the empirical material of the study and analysis on its basis of the attitudes of Russian citizens in relation to the implementation of new management models in a transforming social, economic and political environment. Since the article is based primarily on original empirical material, the presented references to other theoretical and practical works are informative


Author(s):  
П.Е. Гарлов

С целью повышения эффективности искусственного воспроизводства популяций ценных видов рыб разработаны новые методы управления их размножением, выживаемостью и ростом, которые представлены в виде изобретений. Для управления размножением производителей разработаны методы стимуляции и задержки полового созревания производителей осетровых и костистых рыб. Для стимуляции полового созревания были разработаны препараты изолированной передней и изолированной задней долей гипофиза. Их внедрение в осетроводстве позволило повысить степень их рыбоводного использования в среднем на 15% и достичь экономии гипофиза до 40%. Для задержки полового созревания производителей разработан метод их промышленного резервирования в среде критической солёности 4–8‰, причём как в морской воде, так и в растворах поваренной соли. Производственными испытаниями доказана возможность сохранения рыбоводного качества производителей и получения доброкачественного потомства в этой среде при верхних нерестовых температурах в течение производственно необходимых сроков. С целью заводского воспроизводства природных популяций промысловых рыб разработан метод управления их размножением триадой адекватных экологических факторов ‒ «критической» солёностью, температурой и освещённостью при видоспецифических пороговых воздействиях. На этой основе разработан полносистемный метод воспроизводства популяций ценных видов промысловых рыб (севрюги и Балтийского лосося), охватывающий все этапы заводской биотехники. Новый метод осуществляется путём массовой морской заготовки производителей, получения потомства в морских садках и, после заводского речного выращивания молоди до готовности к миграции, конечного доращивания в морских садках крупной жизнестойкой молоди. Для промышленного внедрения всей предложенной биотехники и круглогодичной аквакультуры разработаны системы водоснабжения рыбоводных заводов и рыбоводных хозяйств на основе внесезонного подземного гидрокондиционирования среды выращивания и на природно-промышленных принципах инженерной экологии. In order to increase the efficiency of artificial reproduction of populations of valuable fish species new methods for managing their reproduction, survival and growth have been developed, which are presented in the form of inventions. To control the reproduction of producers methods have been developed to stimulate and delay puberty of sturgeon and bony fish producers. To stimulate puberty preparations for the isolated anterior and isolated posterior lobes of the pituitary gland have been developed. Their introduction in sturgeon breeding made it possible to increase the degree of their fish farming use by an average of 15% and to achieve a pituitary gland economy of up to 40%. To delay the puberty of producers, a method for their industrial reservation in an environment of critical salinity of 4–8‰ has been developed both in sea water and in solutions of sodium chloride. Production tests have proven the possibility of preserving the fish-breeding quality of producers and obtaining benign offspring in this environment at the upper spawning temperatures during the production required periods of time. With the aim of factory reproduction of natural populations of commercial fish a method has been developed to control their reproduction by a triad of adequate ecological factors – “critical” salinity, temperature and illumination under species-specific threshold effects. On this basis a full-system method of reproducing populations of valuable species of commercial fish (stellate sturgeon and Baltic salmon) has been developed covering all stages of factory biotechnology. The new method is carried out by mass marine harvesting of producers obtaining offspring in sea pens and after the factory river rearing of hatchling until ready for migration the final rearing of large viable juveniles in sea pens. For the industrial introduction of all the proposed biotechnics and year-round aquaculture, water supply systems for fish breeding plants and fish farms were developed on the basis of off-season underground hydroconditioning of the growing environment and on the natural and industrial principles of engineering ecology.


Author(s):  
Farhan Yasin Hamed Farhan Yasin Hamed ◽  
Lydia Mikhailovna Vasilyeva ◽  
Natalia Viktorovna Sudakova ◽  
Ali Attala Muheisin ◽  
Adelia Zakirovna Anokhina

The use of the crop rotation method in fish farming makes it possible to increase fish productivity and crop yields in ponds. In the countries of the Middle East, in recent years, the Aqua Crop rotation practice has been developing, in particular, the consistent cultivation of fish and rice in fish ponds, which allows eliminating the contradictions between these crops and increasing production efficiency. In Iraq, an experiment was carried out on the variable cultivation of rice and carp in fish ponds for four years, with the aim of introducing this practice into the country’s agriculture. Four ponds, each with an area of 7.5 hectares, were involved. The research was carried out in two variants: in experiment No.1 rice was grown in the first year, fish in the second, then rice again and in the fourth year — fish, in the second experiment, on the contrary, at the beginning of the fish, then rice, etc. The fish-breeding season lasted 9 months (March to November). Fish-breeding indicators were studied in detail: survival rate, feed costs, fish productivity, the results of rice cultivation were judged by yield. In addition, indicators of economic efficiency were determined according to a simplified scheme. The results indicate that, in general, fish-breeding indicators turned out to be higher when growing fish in ponds after rice, so fish productivity increased by almost 30% with an average value of 3.9 t/ha, feed costs decreased, while rice yield increased by 15–16% and averaged 3.6 t/ha. The best fish-breeding indicators were obtained in the second experiment after rice was grown in the ponds for two years. Economic calculations confirmed the advantages of using the aquacrop rotation practice, so in the second experiment the cost of marketable fish decreased by 10%, and the profitability increased by almost 30%.


2014 ◽  
Vol 42 (2) ◽  
pp. 488-494 ◽  
Author(s):  
DuÅ¡ica DORIC ◽  
Vladislav OGNJANOV ◽  
Mirjana LJUBOJEVIC ◽  
Goran BARAC ◽  
Jovana DULIC ◽  
...  

The paper presents a protocol for micropropagation of Prunus sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversity for rootstock breeding derives from natural populations, where conditions and biological vectors for systematic infection with viral diseases are constantly present. The establishment of aseptic culture depends primarily on the explant type, as all selections were collected from natural habitat. For nearly all investigated selections, dormant buds were the favored source, due to enabling rosette initiation in more than 58% cases. In P. cerasus L. selections, 100% contamination was noted when shoot tips were used as an explant source. Significant influence of the double-phase medium on the number and height of multiplied shoots was observed in the standard cherry rootstock, ‘Gisela 6’. For P. fruticosa Pall., selection ‘SV1’ and ‘SV2’, and P. cerasus ‘D6’ selection, the double-phase medium also had a significant effect on the height of multiplied shoots, when compared to solid DKW (Driver and Kuniyuki Walnut) medium. Genetic variability of selections within the investigated species resulted in variable plant rooting success. Adding Fe-EDDHA (Ethylenediamine di-2-hydroxy-phenyl acetate ferric) in the 200 mg l-1 concentration to the rooting medium significantly enhanced the percentage of rooted plants. The highest rooting percentage was noted for ‘Gisela 6’ and ‘D6’ genotype at 1 mgl-1 IBA (indole-3-butyric acid), while 0.8 mgl-1 was the optimum concentration for P. mahaleb L. ‘M1’ selection. P. fruticosa genotypes required significantly higher IBA concentration for rooting (2.5 and 3.5 mg l-1).


1976 ◽  
Vol 3 (3) ◽  
pp. 349 ◽  
Author(s):  
J Halliday ◽  
JS Pate

'Coralloid' roots containing blue-green algae occur commonly on the upper root stocks of M. riedlei in natural habitat in Western Australia. Each coralloid mass persists for several seasons; replacement sets form at irregular intervals, especially after fire. 15N2 and acetylene reduction assays demonstrate that coralloid roots fix nitrogen at physiologically significant rates. C2H2 reduction rates by coralloid roots are higher in winter than in summer. Performance is positively correlated with rainfall; soil temperature appears to be of lesser importance. Diurnal fluctuations in nitrogenase activity occur. Calibration using 15N2 gives a molar ratio of C2H2 reduced : N2 fixed of 5.8 : 1. The seasonal average of C2H2 reduction of 14.8 nmol per g fresh wt coralloid root per min is then equivalent to 37.6 g N per kg fresh wt per year, a fixation rate potentially capable of doubling coralloid root nitrogen once in every 8 weeks, and whole plant nitrogen every 8-11 years. Returns of fixed nitrogen in two natural populations of Macrozamia are estimated by compounding measurements of biomass of host and symbiotic organs with the seasonal average for coralloid fixation rate. The values obtained (18.8 and 18.6 kg N ha-1 year-1) indicate that Macrozamia contributes significantly to the nitrogen economy of its ecosystem.


2020 ◽  
Vol 200 (4) ◽  
pp. 791-808
Author(s):  
V. N. Koshelev ◽  
N. V. Kolpakov

All available data on sakhalin sturgeon Acipenser mikadoi are summarized. Its historical and modern habitats are described and their significant reduction is noted. Now the species is abundant in the Russian part of its range only — in the Tumnin River, where it is represented in commercial catches by two clearly different groups: large-sized juveniles (FL 43–68 cm) and mature fish (FL 135–169 cm). Juveniles of sakhalin sturgeon migrate during their first years, as their osmoregulatory system forms, to the lower reaches of Tumnin, to the internal estuary, and then to the Datta Bay, the Tatar Strait and the Japan Sea. When returned to the Tumnin, the spawners of sakhalin sturgeon have the length 135–169 cm (n = 29) and weight 15–36 kg. Sex ratio among the caught mature fish is 13.0 : 4 or 3.2 : 1 for females : males, on average. Fecundity of the sturgeon females sampled for artificial reproduction varied from 44.8 to 150.0 thousand eggs, on average 87.3 ± 12.1 . 103 eggs. In total, 17 mature spawners of A.mikadoi were caught in the Tumnin River in 2006–2019 for artificial reproduction (♀ = 13, ♂ = 4), 13 individuals were injected, among them 4/5 of females and 2/3 of males gave high-quality sex products. Producers of this species were distinguished by high survival during manipulations of fish breeding (100 %). Their progeny had low survival, both embryos during incubation and juveniles during rearing; the survival rate for the stage from eggs laid for incubation to juveniles with weight 3.4–7.0 g was 1.85 %. The low survival was supposedly reasoned by combination of unfavorable environmental factors and fish-breeding manipulations. Totally 11,214 juveniles of sakhalin sturgeon with weight from 3.4 to 7.0 g were released into the natural habitat (Tumnin river) in 2007, 2008, 2015, 2017, and 2019. At Anyui sturgeon fish hatchery, the broodstock of sakhalin sturgeon with 274 individuals of 5 ages is created and operated successfully. The male sturgeons in the hatchery mature at the age of 8 years. Here, 11 males of the 2007–2008 year-classes participated in the spawning in 2015–2019. Re-maturation of males was not recorded yet. For the broodstock formation in other fish hatcheries, 200 juveniles of sakhalin sturgeon were transferred to them. To preserve the species, a set of measures is proposed to strengthen its protection and to enhance its artificial reproduction, including the fry releasing into the rivers of the mainland coast, Sakhalin Island, and Japan.


2020 ◽  
Author(s):  
Andrew J. Mongue ◽  
Akito Y. Kawahara

AbstractMany species that are extensively studied in the laboratory are less well characterized in their natural habitat, and laboratory strains represent only a small fraction of the variation in a species’ genome. Here we investigate genomic variation in three natural populations of an agricultural pest and a model insect for many scientific disciplines, the tobacco hornworm (Manduca sexta). We show that hornworms from Arizona, Kansas, and North Carolina are genetically distinct, with Arizona being particularly differentiated from the other two populations. Specifically, two segregating inversions and a potential pseudogene are found only in the Arizona population. One inversion on the Z chromosome may enhance adaptive evolution of the sex chromosome, while the significance of the other, autosomal inversion remains unclear. The pseudogene may be involved in the exploitation of a novel hostplant in Arizona, but functional genetic assays will be required to confirm this hypothesis. Nevertheless, our results reveal undiscovered natural variation and provide useful genomic data for a model insect species.


Author(s):  
Lidia Mihajlovna Vasylieva ◽  
Vasily Vladimirovich Tyapugin ◽  
Natalia Viktorovna Sudakova ◽  
Adelia Zakirovna Anokhina

Formation of beluga brood stocks in artificial conditions whilst a catastrophic state of their natural populations is very timely and relevant. The method of domestication of wild beluga females after intravital obtaining roe was widely used in the Caspian basin, when it was still possible to catch sturgeons in the river. In the Astrakhan region, at the beginning of the current century, that method was considerably used in the formation of beluga brood stocks in cages and ponds after adaptation of fish to artificial conditions of rearing and feeding. At present, the roe obtained from domesticated beluga females is used both for seed production and for caviar production. In addition to studying the fish-breeding indicators of beluga females after obtaining roe, it was of interest to assess the economic efficiency in different farming conditions. The article presents comparative economic indicators of keeping beluga females until maturation in cages and ponds, assessed the total costs, sales income, profit, profitability. The fish-breeding indicators were taken as a basis: total eggs yield, body weight gain, fecundity, the number of oocytes per gram, eggs fertilization ratio, hatching ratio and feed conversion ratio. The article discusses the research data of 15 beluga females in cage conditions and 10 beluga females in ponds. A simplified scheme was adopted in economic calculations, in which it is assumed that feed is a half of the total cost. The results obtained indicate that the profitability of the products obtained from beluga females in cages is an order of magnitude higher than from females in ponds. It was found that the sale of seed production is more profitable than the caviar sales.


2020 ◽  
Vol 2 ◽  
pp. 169-173
Author(s):  
S.I. Melnikov ◽  
◽  
M.V. Shchipakin ◽  

Sheep farming is one of the leading agricul-tural industries in the Russian Federation. A modern veterinarian needs to know the functional principles of all organs and sys-tems of the animal body to understand the etiology and build cause-and-effect relation-ships of the occurrence of certain non-infectious diseases. Most of these diseases occur in the digestive tract (stomach, intes-tines). The purpose of this study is to study in detail the age-related patterns of blood supply of the stomachs of edilbaev sheep. The basis for the research at the Department of animal anatomy of the Saint Petersburg state university of veterinary medicine was post mortem material from animals slaugh-tered at a farm in the Leningrad region. Three age groups were formed. The first group included newborn animals. The sec-ond group included young animals of five to six months. The third group consisted of sheep from one year and older. The age of the animals was determined according to the data obtained from monitor cards. Dur-ing the study, a set of anatomical methods was used, including: fine anatomical dissec-tion, vaso-rentgenography, and photo- graphing. Based on our research, we first established the architectonics of the arterial system of the stomachs of edilbaev sheep in the age aspect. These features have common anatomical patterns of the course and branching of blood vessels, typical to rumi-nants. However, the edilbaev sheep breed is characterized by breed features of skeletal and vascular syntopia , determined by their genetic predisposition. The main arterial line of the multicameral stomach of edilbaev sheep is the ventral artery, which in the course of its topography is divided into additional incoming vessels for all the pre-ventricules and the true stomach. The main morphometric parameters of vascular diameter reach their maximum values by the age of one year and older, while the most intensive increase in the caliber of arterial vessels of the multicameral stomach is typical for the age period from the newborn period to five to seven months.


2003 ◽  
Vol 81 (8) ◽  
pp. 805-813 ◽  
Author(s):  
Hannele Lindqvist-Kreuze ◽  
Hilkka Koponen ◽  
Jari P.T Valkonen

The levels of genotypic and genetic variation were estimated in six natural populations of arctic bramble (Rubus arcticus L. subsp. arcticus) in Finland. Using three primer combinations, a total of 117 amplified fragment length polymorphisms (AFLP) were found. The results were highly reproducible and allowed identification of 78 genets among the 122 plants of arctic bramble studied. Genotypic variation measured as Simpson index (D) was high in all populations, ranging from 0.72 to 0.94. Also, the level of genetic variation measured as Shannon index was relatively high in all populations, ranging from 0.19 to 0.32 (average 0.26). The high levels of genetic diversity indicate that sexual reproduction has played a significant role in these populations. The hierarchical analysis of molecular variance (AMOVA) partitioned 48% of the genetic variation among populations, suggesting a high level of population differentiation and a low level of interpopulation gene flow. Genetic diversity among ten currently grown cultivars of arctic bramble and hybrid arctic bramble (R. arcticus subsp. arcticus × R. arcticus subsp. stellatus) was large, and the subspecies were clearly distinguished from each other based on the AFLP marker data.Key words: AFLP, AMOVA, population, natural habitat, Rubus arcticus subsp. arcticus, Rubus arcticus subsp. stellatus.


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