AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DAN KULIT BATANG BERENUK TERHADAP Bacillus cereus

ABSTRACTThe plants (Crecentia cujete Linn) Are known to have antibacterial activity and can be used as a diarrhea drug due to microorganisms. Bacillus cereus is one of the bacteria that causes diarrhea.This aims to the study was antibacterial activity of ethanol extract of leaves (EL) and bark broodstock (EB) on Bacillus cereus. EL and EB were extracted with ethanol 96% using maceration method. Antibacterial activity test was carried out with disk diffusion method in EL with a concentration of 30%, 60%, 90%, 120% and 150%, and in EB using concentrations of 60%, 70%, 80%, 90% and 100% then antibacterial activity measurements were taken. by looking at the Diameter of the Inhibitory Area (DDH). The results of the EL and EB of antibacterial activity proved that EL and EB berenuk have antibacterial activity against Bacillus cereus.Keywords: Antibacterial, Crescentia cujete Linn, Bacillus cereus.

Download Full-text

Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria

Journal of Pharmaceutical And Sciences ◽

10.36490/journal-jps.com.v3i2.51 ◽

2020 ◽

Vol 3 (2) ◽

pp. 93-99

Author(s):

Athaillah Athaillah ◽

Ugi Diana Lestari

Keyword(s):

Antibacterial Activity ◽

Bacillus Cereus ◽

Allium Sativum ◽

Ethanol Extract ◽

Diffusion Method ◽

Phytochemical Screening ◽

Disk Diffusion Method ◽

Activity Test ◽

Inhibitory Power ◽

Dried Extract

This research is aimed to find out the extract activity dried simplisia of garlic (Allium sativum L.) as an antibacterial which is capable to inhibiting growth of Bacillus cereus bacterium, to find out the alkaloid secondary of metabolites, flavonoid, tannin and saponin that contained in dried extract of garlic and to find out a concentration that has the most antibacterial activity which is capable to inhibiting growth of Bacillus cereus bacterium. Garlic was extracted by maceration method using a solvent ethanol 96%. After the extract obtaned, then phtochemical screening and standardization test. Thickening technique by evaporation used vacum rotary evaporator until thick extarct was obtained. Antibacterial activity thest by using disk diffusion method. This research was used seven concentrations which were 20% (b/v), 30% (b/v). 40% (b/v), 50% (b/v), 60% (b/v), 80% (b/v)and100% (b/v). The result of phytochemical screening test, ethanol extract garlic (Allium sativum L.) positive contained compound alkaloid, flavonoid, saponin and tannin. Standardization of power simplisia fulfill the requirements set by Depkes RI 2000. The result of activity test of optimum antibacterial was obtained inhibitory power with the number 27 mm on 40% (b/v) concentration. This proves that garlic (Allium sativum L.) have antibacterial affects against Bacillus cereus bacterium

Download Full-text

Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.58528 ◽

2021 ◽

Vol 6 (1) ◽

pp. 58528

Author(s):

Vilya Syafriana ◽

Renita Noviani Purba ◽

Yayah Siti Djuhariah

Keyword(s):

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Propionibacterium Acnes ◽

Ethanol Extract ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Activity Test ◽

Etlingera Elatior

This study aimed to determine the antibacterial activity from the ethanol extract of Kecombrang flower (Etlingera elatior (Jack) R.M. Smith) against Staphylococcus epidermidis and Propionibacterium acnes. The extract was made by the maceration method with 70% ethanol as a solvent. Antibacterial activity test was carried out by the disk diffusion method with a concentration of 10%, 20%, 40%, and80%. Meanwhile, theMinimum InhibitoryConcentration(MIC)was done atconcentrations of 10%, 8%, 6%, 4%, and 2%. The results showed that the Kecombrang flower (Etlingera elatior (Jack) R.M.Smith) extract hadantibacterialactivityagainstS.epidermidisand P. acnes. The MIC for S. epidermidis is at a concentration of 4%, while in P.acnes cannot determine yet.

Download Full-text

Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

Jurnal Kesehatan ◽

10.26630/jk.v9i3.967 ◽

2018 ◽

Vol 9 (3) ◽

pp. 336

Author(s):

Ni Luh Budi Artaningsih ◽

Nur Habibah ◽

Mastra Nyoman

Keyword(s):

Antibacterial Activity ◽

Streptococcus Mutans ◽

Ethanol Extract ◽

Inhibition Zone ◽

Gliricidia Sepium ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone

Streptococcus mutans is a positive gram bacteria which cause dental caries. From the several previous studies, Gamal leaf has been predicted as the antibacterial agent because of their active substance such as tannins, alkaloids, saponins, and flavonoids. The objective of the study was to know the antibacterial activity of ethanol extract of Gamal leaf (Gliricidia sepium) for Streptococcus mutans in various concentrations. This study was a true experimental with post-test only control design. The determination of antibacterial activity in this study was conducted by using Kirby Bauer disk diffusion method with the various concentrations, there were 40, 50, 60, 70 and 80%. The positive and negative controls in this study were 30 µg disk diffusion of chloramphenicol and the 96% of ethanol. The average inhibition of zone diameter in the concentrations of 40, 50, 60, 70 and 80% were 11.3, 12.3, 13.4, 15.3 and 19.2 mm, respectively. One Way ANOVA statistical analysis showed that the value of p was 0.000, so there was a difference of growth inhibition zone of Streptococcus mutans in various concentrations of ethanol extract of Gamal leaf (Gliricidia sepium). Based on the result, it can be concluded that the ethanol extract of Gamal leaf (Gliricidia sepium) have strong antibacterial activity, especially to Streptococcus mutans bacteria.

Download Full-text

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽

10.35799/pha.9.2020.31360 ◽

2020 ◽

Vol 9 (4) ◽

pp. 525

Author(s):

Rafi’a Adinda Putri ◽

Herny E.I. Simbala ◽

Deby A. Mpila

Keyword(s):

Antibacterial Activity ◽

Ethanol Extract ◽

Inhibition Zone ◽

Salmonella Typhi ◽

Diffusion Method ◽

Disk Diffusion Method ◽

E Coli ◽

Activity Test ◽

Paper Disk ◽

Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has benefits for many diseases, one of them is antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

Download Full-text

AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽

10.23917/biomedika.v4i2.252 ◽

2012 ◽

Vol 4 (2) ◽

Author(s):

Dr. Muhtadi , MSi. ◽

Ria Ambarwati ◽

Ratna Yuliani

Keyword(s):

Antibacterial Activity ◽

Klebsiella Pneumoniae ◽

Ethyl Acetate ◽

Staphylococcus Epidermidis ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

Download Full-text

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

JOURNAL OF PHARMACY SCIENCE AND TECHNOLOGY ◽

10.30649/pst.v2i1.99 ◽

2020 ◽

Vol 2 (1) ◽

pp. 102

Author(s):

Yanu Andhiarto ◽

Rina Andayani ◽

Nur Hidayatul Ilmiyah

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Skin Inflammation ◽

Screening Tests ◽

Diffusion Method ◽

Moderate Activity ◽

Phytochemical Screening ◽

Strong Activity ◽

Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

Download Full-text

UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽

10.22487/bioceb.v15i2.15540 ◽

2022 ◽

Vol 15 (2) ◽

pp. 90-97

Author(s):

Gaby Maulida Nurdin

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Lantana Camara ◽

Diffusion Method ◽

E Coli ◽

Activity Test ◽

Growth Inhibitory ◽

One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

Download Full-text

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

Pharma Xplore : Jurnal Ilmiah Farmasi ◽

10.36805/farmasi.v4i1.618 ◽

2019 ◽

Vol 4 (1) ◽

pp. 278-287

Author(s):

Lia Fikayuniar ◽

Neni Sri Gunarti ◽

Mellya Apriliani

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Curcuma Longa ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus, ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,

Download Full-text

Antibacterial activity of cis- and trans-resveratrol isolated from Polygonum cuspidatum rhizome

Acta periodica technologica ◽

10.2298/apt0637131k ◽

2006 ◽

pp. 131-136 ◽

Cited By ~ 3

Author(s):

Zoran Kukric ◽

Ljiljana Topalic-Trivunovic

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Antibacterial Effect ◽

Disk Diffusion ◽

Diffusion Method ◽

Polygonum Cuspidatum ◽

Disk Diffusion Method ◽

Cis And Trans

The work is concerned with the antibacterial effect of ethanolic solutions of cis- and trans-resveralrol (cis-.lrans-3,5.4'-trihydroxystilbene) obtained by the extraction (ethanol-water 1:1 v/v) of Polygonum cuspidatum rhizome. Antibacterial activity was tested by disk diffusion method on the following bacteria: Escherichia coli, Sarcina liitea. Bacillus subtilis and Staphylococcits sp., using extract concentrations of 5 mg/disk. All tests showed significant antimicrobial activity, whereby the extract with trans-resveratrol exhibited more significant effect than the extract of cis-resveratrol.

Download Full-text

Skrining Aktivitas Antibakteri Ekstrak Etanol Daun Terhadap Salmonella Typhi Resisten Kloramfenikol

JPSCR Journal of Pharmaceutical Science and Clinical Research ◽

10.20961/jpscr.v2i02.14386 ◽

2017 ◽

Vol 2 (02) ◽

pp. 66

Author(s):

Ika Trisharyanti

Keyword(s):

Antibacterial Activity ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Ethanol Extract ◽

Salmonella Typhi ◽

Diffusion Method ◽

Dilution Method ◽

Tea Leaves ◽

Disk Diffusion Method ◽

Phaleria Macrocarpa

Typhoid fever is an infectious disease caused by Salmonella typhi, antibiotic chloramphenicol can be use to treatment, but S. typhi resistant with antibiotic choramphenicol so need to alternative treatment. The purpose of this study was to determine the antibacterial activity of ethanol extract of ten leaves against Salmonella typhi, and knowing the compounds contained in extracts of leaves that have the best antibacterial activity. The leaves was extracted with ethanol 96% by maceration method. Screening antibacterial activity used disk diffusion method with 10% extract concentration. The best antibacterial activity was determined Minimum Inhibitory Concentration and Minimum Bactericidal Concentration used liquid dilution method, identification test of compounds with thin-layer chromatograpy (TLC), and bioautografi test. There were six extracts had antibacterial activity against S. Typhi, Phaleria macrocarpa leaves, Acalypha siamensis leaves, cherry leaves, clove leaves, tea leaves, and Eugenia polyantha leaves. Minimum Bactericidal Concentration from clove leaf extract was 2,5%. The results of identification by TLC compounds were alkaloids, phenolics, flavonoids, terpenoids, triterpenoids, and saponins. Based on bioautografi test, phenolic can inhibit the growth of Salmonella typhi.

Download Full-text