Effects of mulberry extract on the liver pathology and serum biochemical parameters in carmustine administrated rats

2021 ◽  
pp. 1-13
Author(s):  
Volkan Ipek ◽  
Burcu Menekse Balkan ◽  
Muhammed Enes Inanc ◽  
Oguzhan Kaplan ◽  
Orhan Corum ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adenosine Deaminase ◽  
Recovery Period ◽  
Adenosine Deaminase Activity ◽  
Significant Difference ◽  
Apoptotic Effect ◽  
White Mulberry ◽  
Biochemical Analyses ◽  
Induced Apoptosis ◽  
Oxidative Effects

BACKGROUND: Carmustine is a chemotherapeutic agent that is mainly used in the treatment of glioblastoma and can cause toxic effects on various organs, including the liver. The white mulberry extract has anti-apoptotic and anti-oxidant effects. OBJECTIVE: The study aimed at investigating the effects of the dried white mulberry extract on the pathology, apoptosis, and oxidative stress in the liver, as well as the levels of serum adenosine deaminase, glutathione peroxidase, superoxide dismutase, ceruloplasmin, paraoxonase, and malondialdehyde in carmustine-administrated rats. METHODS: Forty-two rats divided into six groups were used in this study. BCNU was administrated intraperitoneally (IP) (5 mg/kg body weight (BW)/week) for 10 weeks to the BCNU and BCNU-DWME groups. DWME was administered (600 mg/kg-BW by oral gavage) daily for 10 weeks to the DWME and BCNU-DWME groups. After the experimental procedure, histopathological, immunohistochemical, and biochemical analyses were performed. RESULTS: Carmustine caused biliary hyperplasia at a dose of 5 mg/kg. However, the mulberry extract was not effective in alleviating this pathology. Furthermore, the administration of carmustine induced apoptosis in hepatocytes, and the mulberry extract had an anti-apoptotic effect. Carmustine increased the 8-OHdG activity in the liver, and dried mulberry extract ameliorated this activity. Although there was no significant difference in the serum oxidative stress parameters between the groups, carmustine significantly increased the adenosine deaminase activity during the recovery period, while mulberry extracts partially ameliorated these effects in the recovery period. CONCLUSIONS: Dried white mulberry extract has anti-apoptotic and anti-oxidative effects against carmustine-induced toxicity.

scholarly journals Serum total adenosine deaminase activity in Nepalese patients with Rheumatoid Arthritis

2013 ◽  
Vol 4 (2) ◽  
pp. 30-35
Author(s):  
N Gautam ◽  
J Archana ◽  
R Kumar ◽  
LI Singh ◽  
RM Sapkota ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Adenosine Deaminase ◽  
Healthy Controls ◽  
Medical Sciences ◽  
Potential Marker ◽  
Diagnostic Potential ◽  
Clinical Scenarios ◽  
Adenosine Deaminase Activity ◽  
Significant Difference ◽  
Reliable Marker

Objective: Several studies indicate that serum adenosine deaminase (ADA) activity could be a potential marker for the diagnosis of patients with rheumatoid arthritis (RA). However, there has been no such study that could independently verify this finding in Nepali population. The present study therefore aims to measure the total ADA activity in the sera of Nepalese RA patients and verify its diagnostic potential. Materials and Methods: A total of 69 RA patients who visited Universal College of Medical Sciences Teaching Hospital (UCMSTH), Bhairahawa, Nepal for their medical treatment were enrolled for this study. An equal number of age and sex-matched healthy controls were also included in the study. Blood samples were collected from each study subjects and analyzed for serum total ADA, Creactive protein (CRP) and rheumatoid factor (RF). Results: Serum total ADA activity was found to be significantly (p<0.0001) higher (30.0 }10.1 U/L) in all RA patients compared to healthy controls (13.5 } 3.6 U/L). However, no significant difference (p>0.05) in the ADA activity was found between the smokers and non-smoker RA patients. Out of total 69 RA patients, only 16 (23.1%) were positive for CRP and 11 (15.9%) were positive for RF. Conclusion: Measurement of serum total ADA activity could be a reliable marker for the diagnosis of RA in Nepali population with relevant clinical scenarios when there is absence of CRP and RF in the serum.  DOI: http://dx.doi.org/10.3126/ajms.v4i2.6208 Asian Journal of Medical Sciences 4(2013) 30-35

scholarly journals Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

2021 ◽  
Vol 10 (4) ◽  
pp. 328-339
Author(s):  
Bahare Heydari ◽  
◽  
Mohsen Ghofrani ◽  
Mohammad Ebrahim Bahram ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Recovery Period ◽  
Serum Levels ◽  
The Body ◽  
Exhaustive Exercise ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Significant Difference

Objective: The production of reactive oxygen species in exercise causes oxidative stress which disturbs the balance of oxidants and antioxidants, causing destructive effects on cells. The present study aims to investigate the effect of three types of massage (Swedish, Russian, Thai) on serum levels of Malondialdehyde (MDA), Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) following one session of exhaustive exercise. Methods: This quasi-experimental study was conducted on 48 female futsal players aged 17-22 years in Zahedan, Iran who were selected using a purposive sampling method, and randomly divided into four groups of Swedish massage (Long strokes with pressing and tapping using hands), Russian massage (Medium to high pressure), Thai massage (Pressure to certain parts of the body) and Control. The exercise program was based on Bruce protocol. Serum levels of MDA, GPX and SOD were measured by before and immediately after exercise and after massage. Data analysis was performed using repeated measures ANOVA, considering a significance level of P≤0.05. Results: In all three types of massage, there was a significant decrease in serum level of MDA (0.22±0.08), and a significant increase in GPX (1.84±0.46) and SOD (10.02±2.86) levels after exhaustive (P=0.001). No significant difference was observed in the control group. Conclusion: It seems that Russian, Thai, and Swedish types of massage can affect the serum levels of the MDA (as an oxidative stress marker) and the antioxidant enzymes of GPX and SOD during the post-exercise recovery period.

scholarly journals Diagnostic significance of pleural fluid adenosine deaminase activity in tuberculous pleurisy

1970 ◽  
Vol 5 (1) ◽  
pp. 1-5
Author(s):  
Sharmeen Ahmed ◽  
Reaz Fatema ◽  
Ahmed Abu Saleh ◽  
Mumayun Sattar ◽  
Md Ruhul Amin Miah
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Colorimetric Method ◽  
Diagnostic Methods ◽  
Test Accuracy ◽  
Diagnostic Significance ◽  
Tuberculous Pleural Effusion ◽  
Adenosine Deaminase Activity ◽  
Significant Difference

Diagnosis of tuberculous pleural effusion (TPE) is difficult because of its non-specific clinical presentation and insufficient efficiency of conventional diagnostic methods. The study was carried out to evaluate the utility of adenosine deaminase (ADA) activity in pleural fluid for the diagnosis of TPE. ADA activity was measured in pleural fluid of 103 pleural effusion patients by colorimetric method using a commercial ADA assay kit. The diagnosis of TPE was made from pleural fluid examinations (including cytology, biochemistry, and bacteriology) and pleural biopsy. Patient with negative result of this methods were diagnosed by response of empirical treatment. Out of 130 cases, 62 (61.1%) had TPE and the remaining 41 (39.8%) had pleural effusion due to non tuberculous diseases. There was statistically significant difference (p < 0.001) between the mean of pleural fluid ADA levels (70.82±22.54 U/L) in TPE group and (30.07±22.93 U/L) in non-TPE group. Of 62 TPE cases, microscopy for AFB and culture for M.tuberculosis in pleural fluid revealed positivity in 9.6% and 22.5% cases respectively, and biopsy of pleura showed typical epithelioid granuloma in only 43.5% cases. The cut-off value of ADA for diagnosing TPE was 40 U/L using a ROC curve, with a sensitivity of 94% and specificity of 88%. Positive and negative predictive value of ADA assay were 92% and 90% respectively. The overall test accuracy was 90%. Pleural fluid ADA assay is therefore a simple, rapid, highly sensitive and specific adjunct test for diagnosis of TPE. DOI: http://dx.doi.org/10.3329/imcj.v5i1.9852   Ibrahim Med. Coll. J. 2011; 5(1): 1-5 Keywords: Pleural fluid; adenosine deaminase; tuberculous pleural effusion

Oxidative stress and changes in adenosine deaminase activity of cattle experimentally infected by Fasciola hepatica

Parasitology ◽  
2017 ◽  
Vol 144 (4) ◽  
pp. 520-526 ◽  
Author(s):  
ALEKSANDRO S. DA SILVA ◽  
MATHEUS D. BALDISSERA ◽  
NATHIELI B. BOTTARI ◽  
MATHEUS E. GABRIEL ◽  
LEANDRO A. RHODEN ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adenosine Deaminase ◽  
Liver Tissue ◽  
Fasciola Hepatica ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Adenosine Deaminase Activity ◽  
Group A ◽  
Group B

SUMMARYThe aim of this study was to evaluate the oxidative stress in serum and liver and adenosine deaminase (ADA) activity of cattle experimentally infected by Fasciola hepatica. The group A consisted of five healthy animals (uninfected), and the group B was composed of five animals orally infected with 200 metacercariae of F. hepatica. On days 20, 40, 60 and 80 post-infection (PI) serum was collected to measure oxidative stress variables. On day 100 PI, animals were humanely euthanized and liver samples were collected. Infected animals showed lower (P < 0·05) seric ADA activities on days 40 and 60 PI but higher (P < 0·05) in the liver tissue compared with uninfected animals. Seric and hepatic reactive oxygen species (ROS) were higher (P < 0·05) in infected compared with uninfected animals. Hepatic thiobarbituric acid reactive substances were higher (P < 0·05) in infected animals. Catalase and glutathione S-transferase activities were lower in liver tissue of infected animals, while glutathione peroxidase was higher compared with uninfected (P < 0·05). In summary, we conclude that oxidative stress occurs in cattle experimentally infected by F. hepatica, mainly due to excessive ROS production in the course of fasciolosis, contributing to hepatic damage, and that increased in hepatic ADA activity may contribute to the inflammatory process.

scholarly journals IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K/PDK-1/Akt pathway

2018 ◽  
Vol 7 (3) ◽  
pp. 443-455 ◽  
Author(s):  
Chanyang Kim ◽  
Seungjoon Park
Keyword(s):  
Oxidative Stress ◽  
Citrate Synthase ◽  
Apoptotic Cell ◽  
Parp Cleavage ◽  
Akt Pathway ◽  
Protective Effects ◽  
Cytochrome C Release ◽  
Mitochondrial Ros ◽  
Apoptotic Effect ◽  
Induced Apoptosis

Insulin-like growth factor (IGF)-1 is a well-known anti-apoptotic pro-survival factor and phosphatidylinositol-3-kinase (PI3K)/Akt pathway is linked to cell survival induced by IGF-1. It is also reported that Akt signaling is modulated by 3-phosphoinositide-dependent kinase-1 (PDK1). In the current study, we investigated whether the anti-apoptotic effect of IGF-1 in SH-SY5Y cells exposed to 1-methyl-4-phenylpyridinium (MPP+) is associated with the activity of PI3K/PDK1/Akt pathway. Treatment of cells with IGF-1 inhibited MPP+-induced apoptotic cell death. IGF-1-induced activation of Akt and the protective effect of IGF-1 on MPP+-induced apoptosis were abolished by chemical inhibition of PDK1 (GSK2334470) or PI3K (LY294002). The phosphorylated levels of Akt and PDK1 were significantly suppressed after MPP+ exposure, while IGF-1 treatment completely restored MPP+-induced reductions in phosphorylation. IGF-1 protected cells from MPP+ insult by suppressing intracellular reactive oxygen species (ROS) production and malondialdehyde levels and increasing superoxide dismutase activity. Mitochondrial ROS levels were also increased during MPP+ exposure, which were attenuated by IGF-1 treatment. In addition, IGF-1-treated cells showed increased activities of succinate dehydrogenase and citrate synthase, stabilization of mitochondrial transmembrane potential, increased ratio of Bcl-2 to Bax, prevention of cytochrome c release and inhibition of caspase-3 activation with PARP cleavage. Furthermore, the protective effects of IGF-1 on oxidative stress and mitochondrial dysfunction were attenuated when cells were preincubated with GSK2334470 or LY294002. Our data suggest that IGF-1 protects SH-SY5Y cells against MPP+-associated oxidative stress by preserving mitochondrial integrity and inhibiting mitochondrial apoptotic cascades via the activation of PI3K/PDK1/Akt pathway.

scholarly journals Oxidative Stress and Its Relationship With Adenosine Deaminase Activity in Various Stages of Breast Cancer

2012 ◽  
Vol 28 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Mridula Mahajan ◽  
Nitika Tiwari ◽  
Ritu Sharma ◽  
Sukhraj Kaur ◽  
Neetirajan Singh
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Adenosine Deaminase ◽  
Adenosine Deaminase Activity

scholarly journals 5`-Nukleotidase and adenosine deaminase in patients with rheumatoid arthritis

2017 ◽  
Vol 74 (10) ◽  
pp. 940-946 ◽  
Author(s):  
Nela Zivkovic ◽  
Boris Djindjic ◽  
Sonja Stojanovic ◽  
Ivana Krstic ◽  
Ivana Ciric ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Adenosine Deaminase ◽  
Inflammatory Diseases ◽  
Cell Damage ◽  
Control Group ◽  
Adenosine Deaminase Activity ◽  
Significant Difference ◽  
Post Hoc ◽  
Rheumatoid Arthritis Activity

The essence of rheumatoid arthritis (RA) pathogenesis is inflammation, modification of immune system and cell damage. 5'-nucleotidase (5'-NT) and adenosine deaminase (ADA) have a significant role in the process of inflammation-caused tissue damage. The aim of the paper is to define 5'-nucleotidase and adenosine deaminase activity in serum of patients with rheumatoid arthritis treated with Methotrexate and patients with rheumatoid arthritis who have not been treated with Methotrexate, as well as to determine correlation between the enzymes and disease activity. The research included 160 patients suffering from rheumatoid arthritis, 60 of which have not been treated with Methotrexate (age 56,8; 68,3% female) and 100 patients treated with Methotrexate (age 59,8; 88% female), as well as 60 healthy controls (age 58,8; 66,6% female). Patients suffering from chronic inflammatory diseases, chronic respiratory, cardiac and kidney insufficiency, severe acute diseases and other diseases which might modify inflammatory response were not included in the research. There was no statistically significant difference in 5'-NT values among groups. ADA values were significantly different in all tested groups. Post Hoc analysis (Dunnett T3 test) showed that ADA activity in RA groups were significantly higher as compared to control group (p<0.001), and that ADA in RA group with MTX was significantly smaller as compared to RA group without MTX (p<0.001). There is not significant correlation between the disease activity and activities of tested enzymes. We concluded that adenosine deaminase activity was increased in patients with rheumatoid arthritis, as well as that the application of Methotrexate led to the decrease of this enzyme in the serum of patients with rheumatoid arthritis. Activity of 5'-nucleotidase is not increased in patients with rheumatoid arthritis and did not depend on Methotrexate treatment. Serum adenosine deaminase and 5'-nucleotidase were not good indicators of rheumatoid arthritis activity.

scholarly journals AKTIVITAS FISIK MAKSIMAL AKUT (ACUTE OVERTRAINING) MENYEBABKAN KERUSAKAN SEL β PANKREAS MENCIT

2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Ferbian M. Siswanto ◽  
Boedi P. Yenniastuti ◽  
Tri A. Putra ◽  
I Made Kardena
Keyword(s):  
Oxidative Stress ◽  
Recovery Period ◽  
The Body ◽  
Physical Work ◽  
Type I ◽  
Β Cells ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
One Way Anova

Abstract: Excessive physical activity can cause oxidative stress characterized by increased free radicals in the body. This oxidative stress will cause damages to a variety of cells, inter alia β cells of islets of Langerhans in pancreas. This study aimed to determine that acute overtraining could increase the risk of type I diabetes mellitus through induction of pancreatic β cell damages. This study used a completely randomized design with total samples of 36 mice divided into 3 groups. Group P0 was the group of mice without treatment; group P1 was treated with overworking and a recovery period of 48 hours; and group P2 was treated with overworking and a recovery period of 24 hours. After 14 days of treatment, the pancreas of all groups were taken for histological examination using Gomori chrome hematoxylin phloxine B staining. The descriptive analysis showed that the pancreatic tissues of groups P1 and P2 were morphologically damaged compared to group P0. The results of quantitative observations were analyzed by using One way Anova test followed by LSD, indicated that the number of pancreatic β-cells was significantly decreased among the groups (P < 0.05). Conclusion: Acute overtraining may cause damages of pancreatic β cells.Keywords: excessive physical work, pancreatic β cellsAbstrak: Aktivitas fisik berlebihan dapat menyebabkan terjadinya stres oksidatif yang ditandai dengan meningkatnya radikal bebas di dalam tubuh. Stres oksidatif akan menyebabkan kerusakan berbagai macam sel, salah satunya sel β pulau langerhans pankreas. Penelitian ini bertujuan untuk mengetahui aktivitas fisik maksimal akut (acute overtraining) dalam meningkatkan risiko terjadinya diabetes melitus tipe I melalui induksi kerusakan sel β pankreas. Rancangan penelitian ini ialah completely randomized design dengan sampel 36 ekor mencit yang dibagi menjadi 3 kelompok. Kelompok P0 ialah kelompok mencit tanpa perlakuan; kelompok P1 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 48 jam; dan kelompok P2 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 24 jam. Setelah 14 hari perlakuan, keseluruhan kelompok sampel diambil organ pankreasnya untuk dibuat sediaan histologik dengan pewarnaan khusus Gomori chrome hematoxylin phloxine B. Analisis deskriptif kualitatif menunjukkan bahwa morfologi jaringan pankreas pada kelompok P1 dan P2 mengalami kerusakan jika dibandingkan P0. Hasil pengamatan kuantitatif dianalisis dengan menggunakan uji One way Anova dilanjutkan dengan Least Significant Difference (LSD), menunjukkan bahwa terjadi penurunan jumlah sel β pankreas yang signifikan antar kelompok P0, P1, dan P2 (P < 0,05). Simpulan: Aktivitas fisik maksimal akut dapat menyebabkan kerusakan sel β pankreas.Kata kunci: beban kerja maksimal, sel β pankreas

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