scholarly journals Post-vaccination immunity in dogs vaccinated and treated against the parvovirus enteritis

2021 ◽  
Vol 2021 (2) ◽  
pp. 19-24
Author(s):  
Vladimir Gerasimchik ◽  
Evgeniy Eremeev
Keyword(s):  
Clinical Examination ◽  
Total Protein ◽  
Health Indicators ◽  
Toxocara Canis ◽  
The State ◽  
Post Vaccination ◽  
Experimental Animals ◽  
Antibody Titers ◽  
Biochemical Research

In this article we present the results of studies aimed at establishing the level of post-vaccination antibodies to parvovirus enteritis. The work was performed on dogs 1.5…2 months old. The animals were divided into two groups. In the first group, dogs were vaccinated and treated against endoparasites according to all instructions. Dogs of the second group were also vaccinated, but were not exposed to anthelmintic treatments. Further, the state of the experimental animals was monitored with the methods of clinical examination, coproscopic, hematological, biochemical and serological researches. Dogs not treated against endoparasites showed symptoms of parvovirus enteritis by the 6th day of the experiment. Coproscopic research indicated an invasion of Toxocara canis by nematodes. Hematological research showed a decrease in the level of hemoglobin, hematocrit and platelets. The results of biochemical research showed a decrease in the level of albumin and total protein. During a serological research (ELISA), the development of extremely low antibody titers was noted. The dogs of the first group did not show any signs of deterioration in health. Indicators of hematology and biochemistry were within the physiological norm. The ELISA results indicated the development of antibodies to parvovirus enteritis. This research confirms the need for anthelmintic treatments before vaccinations.

scholarly journals Diphtheria-tetanus-pertussis vaccine combined with Bordetella parapertussis vaccine

1962 ◽  
Vol 60 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Neda Köhler-Kubelka
Keyword(s):  
Clinical Examination ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Whooping Cough ◽  
Production Test ◽  
Post Vaccination ◽  
Bordetella Parapertussis ◽  
Cross Reactions ◽  
Combined Vaccine

Investigations carried out to ascertain the ability of various strains of Bordetella pertussis and B. parapertussis to produce agglutinins have shown that the agglutinin response is considerably greater with B. parapertussis.Children inoculated with a combined vaccine in which the parapertussis element contained B. parapertussis in only one-twelfth of the concentration of B. pertussis in the pertussis element showed agglutinins in their sera in titres well above 1:300 for both organisms. There were no cross-reactions and the serological responses were specific throughout. The vaccine used was the standard diphtheria-tetanus-pertussis (DTP) prophylactic to which had been added a vaccine prepared from recently isolated strains of B. parapertussis.Agglutinin titres of both whooping cough components with the combined vaccine were somewhat lower in mice than was the case when monovalent vaccines were used, but they were considered to be satisfactory.It is suggested that the agglutination production test in mice could be used for the assessment of protective power of B. parapertussis vaccines against infection.I wish to thank Dr Ikić, director of the Institute of Immunology, Zagreb, who enabled me to perform all these examinations, further to Dr B. Mravunac and Dr Z. Radanov for having carried out vaccination in children and for the clinical examination of post vaccination reactions.

scholarly journals Serosurvey of Rickettsia spp. in dogs and humans from an endemic area for Brazilian spotted fever in the State of São Paulo, Brazil

2008 ◽  
Vol 24 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Adriano Pinter ◽  
Maurício C. Horta ◽  
Richard C. Pacheco ◽  
Jonas Moraes-Filho ◽  
Marcelo B. Labruna
Keyword(s):  
Human Serum ◽  
Endemic Area ◽  
Spotted Fever ◽  
Sao Paulo ◽  
The State ◽  
The Other ◽  
São Paulo ◽  
Human Sera ◽  
Antibody Titers ◽  
Brazilian Spotted Fever

The present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for Brazilian spotted fever in the State of São Paulo, where the tick Amblyomma aureolatum is the main vector. Testing canine and human sera by indirect immunofluorescence against four Rickettsia antigens (R. rickettsii, R. parkeri, R. felis and R. bellii) showed that 16 (64%) of canine sera and 1 (2.8%) of human sera reacted to at least one of these rickettsial antigens with titers <FONT FACE=Symbol>³</FONT> 64. Seven canine sera and the single reactive human serum showed titers to R. rickettsii at least four times those of any of the other three antigens. The antibody titers in these 7 animals and 1 human were attributed to stimulation by R. rickettsii infection. No positive canine or human serum was attributed to stimulation by R. parkeri, R. felis, or R. bellii. Our serological results showed that dogs are important sentinels for the presence of R. rickettsii in areas where the tick A. aureolatum is the main vector of Brazilian spotted fever.

scholarly journals Serological response to rabies virus induced by commercial vaccines in cattle

2017 ◽  
Vol 47 (10) ◽  
Author(s):  
Mathias Martins ◽  
João Motta de Quadros ◽  
Eduardo Furtado Flores ◽  
Rudi Weiblen
Keyword(s):  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Vaccine Dose ◽  
Booster Vaccination ◽  
Serological Response ◽  
Serum Samples ◽  
Anamnestic Response ◽  
Post Vaccination ◽  
Antibody Titers ◽  
One Year

ABSTRACT: The antibody response to rabies virus (RABV) induced by commercial vaccines in heifers was investigated. For this, 84 heifers were vaccinated twice (30 days interval) with each of four vaccines (G1 = 14 animals; G2 = 24; G3 = 22 and G4 = 24) and received a booster vaccination 360 days later. Serum samples collected at different intervals after vaccination and 30 days after booster were submitted to a virus neutralizing (VN) assay for RABV antibodies. Thirty days after the second vaccine dose, 92% of the immunized animals presented VN titers ≥0.5UI/mL (geometric medium titers [GMT] 1.7 to 3.8UI/mL). At the day of the booster (360 days post-vaccination); however, the percentage of animals harboring antibody titers ≥0.5UI/mL had dropped to 31% (0-80% of the animals, depending on the vaccine), resulting in lower GMT (0.1 to 0.6UI/mL). Booster vaccination at day 360 resulted in a detectable anamnestic response in all groups, resulting in 83% of animals (65 to 100%) harboring VN titers ≥0.5UI/mL thirty days later (GMT 0.6 to 4.3UI/mL). These results indicated that these vaccines were able to induce an adequate anti-RABV response in all animals after prime vaccination (and after booster as well). However, the titers decreased, reaching titers <0.5UI/mL in approximately 70% of animals within the interval before the recommended booster. Thus, booster vaccination for rabies in cattle using the current vaccines should be performed before the recommended one-year interval, as to maintain neutralizing antibodies levels in most vaccinated animals.

scholarly journals SARS-CoV-2 Post Vaccinated Adverse Effects and Efficacy in the Egyptian Population

Vaccines ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Marwa O. Elgendy ◽  
Ahmed O. El-Gendy ◽  
Abdulaziz Ibrahim Alzarea ◽  
Sarah Mahmoud ◽  
Saad S. Alqahtani ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Laboratory Tests ◽  
Dose Interval ◽  
Vaccine Safety ◽  
Spike Protein ◽  
Post Vaccination ◽  
The Third ◽  
Antibody Titers ◽  
Administration Methods ◽  
Structured Questionnaire

Vaccines are the solution to overcome SARS-CoV-2. This study aimed to determine the post-Sinopharm vaccine safety-profile and immunity through antibody titers. Data were collected using a structured questionnaire from Egyptian participants who received two doses of Sinopharm vaccine. Data were divided into three parts, the first and second parts were to detect participants’ post-first and second dose symptoms and practices, and the third for the results of IgG anti spike protein antibodies test and laboratory tests. Pain, redness, swelling at the injection site, headache, fatigue, and lethargy were the most common post-vaccine symptoms for both first and second doses. Most of the participants felt mild or no symptoms after vaccination. The symptoms started mostly during the first day post-vaccination and lasted for no more than two days. Forty-nine percent of the participants resulted in positive antibodies tests on day 18 post-vaccination. The average antibody level for vaccinated participants with past SARS-CoV-2 infection was much higher than that for non-past infected participants. These vaccines’ administration methods need to be reevaluated by changing the dose, dose interval, adding a third dose, or mixing it with other vaccines with different techniques to improve their protection rates. Further studies are required to validate this finding.

scholarly journals The research of the state of prosthetic bed tissues in patients with pemphigus vulgaris.

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
Bohdan Lyubomyrovych Henyk ◽  
Mykola Mykhaylovych Rozko
Keyword(s):  
Survey Data ◽  
Oral Mucosa ◽  
Clinical Examination ◽  
Comparative Evaluation ◽  
Objective Assessment ◽  
Pemphigus Vulgaris ◽  
The State ◽  
Control Group

The clinical examination of condition of tissues prosthetic bed was conducted in 20 patients with  pemphigus vulgaris. The results are compared with survey data of 20 persons of control group without somatic pathology. It was conducted the analyzes of subjective and objective assessment of tissues prosthetic bed, the results of clinical examination and frequency of various pathologies of the oral mucosa membrane in the studied groups, conducted the comparative evaluation of dental indicators.

scholarly journals THE EFFECTIVENES OF COMPLEX DRUG “BIFIDUM BAG” FOR STATUS CORRECTION OF LARGE INTESTINE MICROBOICENOSIS AND ANTIOXIDANT PROPERTIES OF COLONOCYTES IN EXPERIMENTAL DYSBIOSIS

2019 ◽  
Vol 23 (2) ◽  
pp. 203-210
Author(s):  
O. A. Medvedeva ◽  
V. A. Korolev ◽  
N. A. Verevkina ◽  
V. A. Riadnova
Keyword(s):  
Superoxide Dismutase ◽  
Large Intestine ◽  
Antioxidant Properties ◽  
Malonic Dialdehyde ◽  
The State ◽  
Control Group ◽  
Experimental Animals ◽  
Bacteriological Method ◽  
Complex Preparation ◽  
Complex Drug

The effectiveness of complex drug “Bifidum BAG” for status correction of large intestine microbiocenosis and antioxidant properties of colonocytes in experimental dysbiosis has been studied. Acomplex drug was administered to experimental animals, which includes Bifidobacterium and Dihydroquercetin. Quantitative and qualitative study of large intestine was done in mices by bacteriological method. The state of lipid peroxidation system was evaluatedaccoding to the content of acylhydroperoxide and malonic dialdehyde. The state of antioxidant protective system was reached by means of catalase and superoxide dismutase activity. Experimental dysbiosis was shown as significant changes in mucosal microflora, changes colonocytes antioxidant properties. The use of the complex preparation “Bifidum BAG”, led to the normalization of the colon microbiota (11 of 16 microorganisms were recovered). After correction gentamicin-associated dysbiosis with a complex probiotic, a positive effect of the drug on the colonocytes antioxidant defense was noted. So the activity of catalase increased 1.1 times, compared with the determined index in the group “dysbiosis”. The activity of superoxide dismutase increased 2 times in comparison with the group “dysbiosis”, exceeding the value of the control group. The concentration of LPO products in colonocytes of experimental animals decreased significantly. The content of malonicdialdehyde and acylhydroperoxide decreased 1.6 times and 5.6 times in comparison with the determined index of the group “dysbiosis”, respectively.

scholarly journals The results of clinical examination of patients with decompensated form of dental abrasion and complaints of the state of the temporomandibular joints

2020 ◽  
Vol 20 (1-2) ◽  
pp. 80-84
Author(s):  
Rustem R. Gabdrafikov
Keyword(s):  
Clinical Examination ◽  
The State ◽  
Old Patients ◽  
Temporomandibular Joints

We examined 66 patients 1755 years old with decompensated form of dental abrasion, who had applied to the hospital Dental Forte with various types of complaints of discomfort in the TMJ. They were divided into several groups: 1st 1725 years old, 2nd 2535 years old, 3rd 3545 years old, 4th 4555 years old patients. It was established, that the age of 2535 is specific for significant indication of dysfunctions in the TMJ. By the age of 3545 years the number of complaints of discomfort in the TMJ increases. There is also increase in the diversity of complaints, and decrease in their allocation in percentage terms. The number of complaints and their diversity in patients of 4555 years old remained, complaints of one type of pain decreased, but the number of complaints about pain associated with chewing, headache, crunch, etc. increased.

scholarly journals Characterization of the significant decline in humoral immune response six months post-SARS-CoV-2 mRNA vaccination: A systematic review

2021 ◽  
Author(s):  
Kin Israel Notarte ◽  
Israel Guerrero-Arguero ◽  
Jacqueline Veronica Velasco ◽  
Abbygail Therese Ver ◽  
Maria Helena Santos de Oliveira ◽  
...  
Keyword(s):  
Humoral Response ◽  
Serum Levels ◽  
Related Factors ◽  
Post Vaccination ◽  
Humoral Immune ◽  
Antibody Titers ◽  
Waning Immunity ◽  
Antibody Class ◽  
Antibody Decline ◽  
Primary Cycle

Accumulating evidence shows a progressive decline in the efficacy of coronavirus disease 2019 (COVID-19) mRNA vaccines such as Pfizer-BioNTech (mRNA BNT161b2) and Moderna (mRNA-1273) in preventing breakthrough infections due to diminishing humoral immunity over time. Thus, this review characterizes the kinetics of anti-SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) antibodies after the second dose of a primary cycle of COVID-19 mRNA vaccination. A systematic search of literature was performed and a total of 18 studies (N=15,980) were identified and reviewed. The percent difference of means of reported antibody titers were then calculated to determine the decline in humoral response after the peak levels post-vaccination. Findings revealed that the peak humoral response was reached at 21-28 days after the second dose, after which serum levels progressively diminished at 4-6 months post-vaccination. Additionally, results showed that regardless of age, sex, serostatus and presence of comorbidities, longitudinal data reporting antibody measurement exhibited a decline of both anti-receptor binding domain (RBD) IgG and anti-spike IgG, ranging from 94-95% at 90-180 days and 55-85% at 140-160 days, respectively, after the peak antibody response. This suggests that the rate of antibody decline may be independent of patient-related factors and peak antibody titers but mainly a function of time and antibody class/molecular target. Hence, this study highlights the necessity of more efficient vaccination strategies to provide booster administration in attenuating the effects of waning immunity, especially in the appearance of new variants of concerns (VoCs).

scholarly journals Detection of buffaloes (Bubalus bubalis) seroreactive for vesicular stomatitis virus in the state of Paraíba, Northeastern Brazil

2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3769
Author(s):  
Camila de Sousa Bezerra ◽  
Jéssica Tatiane Sauthier ◽  
Juliana Felipetto Cargnelutti ◽  
Gisele Cândida Ramalho ◽  
Denise Batista Nogueira ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Virus Isolation ◽  
Neutralization Test ◽  
Bubalus Bubalis ◽  
The State ◽  
Virus Neutralization Test ◽  
Northeastern Brazil ◽  
Antibody Titers ◽  
Vesicular Stomatitis

This study aimed to determine the prevalence of vesicular stomatitis virus (VSV) in buffaloes in the state of Paraíba, Northeastern Brazil. The study was carried out in 14 herds in the municipalities of Alagoa Nova, Areia, Campina Grande, Guarabira, Juripiranga, Santa Helena, Sapê, Rio Tinto, Santana dos Garrotes, Itatuba, Solânea, and Cacimbas. The studied population was formed by buffalo females reared for meat and milk, comprising of mixed and Murrah breeds, at least 24 months of age. For the serological diagnosis of VSIV-3, the virus-neutralization test (VN) was performed, using the VSIV-3 isolate 2013 São Bento/Paraíba. Real prevalence was 2.6% for VSIV-3, with antibody titers ranging from 160 to 1280. This is the first study to characterize VSV circulation in the buffalo population in Northeastern Brazil, where infection is considered endemic; some aspects of virus maintenance are not fully understood, such as the role of reservoirs in endemic areas. The identification of seroreactive animals in this study demonstrates the circulation of VSIV-3 in the buffalo species. Reports of virus isolation in this species have not yet been described, which suggests the need for investigating the role of buffaloes in vesicular stomatitis epidemiology.

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