scholarly journals CONSIDERATIONS OF MORPHOLOGIC OBSERVATIONS OF MOSQUITO SPECIES FROM IDENTIFYING COMPLETE SAMPLES IN PANAMA CITY BEACH, FLORIDA

2021 ◽  
Vol 68 (1) ◽  
pp. 6-13
Author(s):  
Michael T. Riles
Keyword(s):  
Public Health ◽  
Mosquito Species ◽  
Abundant Species ◽  
Geographic Range ◽  
Similar Species ◽  
Panama City ◽  
Species Specific ◽  
Control Application ◽  
Identification Practices ◽  
Data Banks

In Panama City Beach, Florida, thirteen mosquito species have been recently registered into public health data banks over the span of 7 years [2014-2020], ten species within their published geographic range and three species outside of their noted geographic range. The underreporting is likely due to past identification practices of sub-sampling and aliquoting surveillance collections while only recording the top-most three abundant species for control application thresholds. However, these thirteen species have not been recorded in this area by public health operations up until their respective record timelines. Timelines of identification, species specific character states, the dynamic of identifying similar species and alternate identification methods are discussed. As of 2020, 10 genera and 50 species within Diptera: Culicidae are recorded in Panama City Beach, FL, U.S.A.

scholarly journals Responses of Bat Communities (Mammalia: Chiroptera) to Forest Loss and Habitat Conversion in Southern Cameroon

2021 ◽  
Vol 14 ◽  
pp. 194008292110103
Author(s):  
Patrick Jules Atagana ◽  
Eric Moïse Bakwo Fils ◽  
Sevilor Kekeunou
Keyword(s):  
Secondary Forest ◽  
Biosphere Reserve ◽  
Abundant Species ◽  
Primary Forest ◽  
Habitat Types ◽  
Habitat Transformation ◽  
Significant Difference ◽  
Southern Cameroon ◽  
Habitat Conversion ◽  
Species Specific

We aimed to assess how bats are affected by habitat transformation by comparing bat assemblages in four habitat types: primary forest, secondary forest, cocoa plantations and human habitations in the Dja Biosphere Reserve of southern Cameroon. Bats were sampled in the four habitat types using mist nets. During 126 nights, a total of 413 bats were captured, belonging to four families, 16 genera and 24 species. Ninety three individuals (17 species) were captured in the primary forest, followed by plantations (105 individuals, 14 species), human habitations (159 individuals, 10 species), and secondary forest (55 individuals, eight species). Megaloglossus woermanni was recorded in all the four habitats, and was the most abundant species (105 individuals). The analysis of bat assemblage between habitat types showed a statistically significant difference in species composition. The distribution of the six most abundant species ( Epomops franqueti, Megaloglossus woermanni, Rousettus aegyptiacus, Dohyrina cyclops, Hipposideros cf. caffer and Hipposideros cf. ruber) was influenced by habitat types. Our results suggest that the decrease in species richness observed in disturbed habitats may be due to habitat perturbations of primary forest habitats. Therefore, it is important to examine the effects of habitat conversion at species level, as responses are often species-specific.

scholarly journals The Serological Cross-Detection of Bat-Borne Hantaviruses: A Valid Strategy or Taking Chances?

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1188
Author(s):  
Renata Carvalho de Oliveira ◽  
Jorlan Fernandes ◽  
Elba Regina de Sampaio Lemos ◽  
Fernando de Paiva Conte ◽  
Rodrigo Nunes Rodrigues-da-Silva
Keyword(s):  
Public Health ◽  
In Silico ◽  
Data Bank ◽  
Distinct Species ◽  
Geographic Range ◽  
Ab Initio Methods ◽  
Success Rates ◽  
B Cell Epitopes ◽  
Crystallographic Structures ◽  
A Minor

Bats are hosts of a range of viruses, and their great diversity and unique characteristics that distinguish them from all other mammals have been related to the maintenance, evolution, and dissemination of these pathogens. Recently, very divergent hantaviruses have been discovered in distinct species of bats worldwide, but their association with human disease remains unclear. Considering the low success rates of detecting hantavirus RNA in bat tissues and that to date no hantaviruses have been isolated from bat samples, immunodiagnostic tools could be very helpful to understand pathogenesis, epidemiology, and geographic range of bat-borne hantaviruses. In this sense, we aimed to identify in silico immunogenic B-cell epitopes present on bat-borne hantaviruses nucleoprotein (NP) and verify if they are conserved among them and other selected members of Mammantavirinae, using a combination of (the three most used) different prediction algorithms, ELLIPRO, Discotope 2.0, and PEPITO server. To support our data, we in silico modeled 3D structures of NPs from representative members of bat-borne hantaviruses, using comparative and ab initio methods due to the absence of crystallographic structures of studied proteins or similar models in the Protein Data Bank. Our analysis demonstrated the antigenic complexity of the bat-borne hantaviruses group, showing a low sequence conservation of epitopes among members of its own group and a minor conservation degree in comparison to Orthohantavirus, with a recognized importance to public health. Our data suggest that the use of recombinant rodent-borne hantavirus NPs to cross-detect antibodies against bat- or shrew-borne viruses could underestimate the real impact of this virus in nature.

scholarly journals Retention of relict satellite DNA sequences in Anemone (Ranunculaceae)

2013 ◽  
Vol 72 (1) ◽  
pp. 1-133 ◽  
Author(s):  
Višnja Besendorfer ◽  
Jelena Mlinarec
Keyword(s):  
Dna Sequences ◽  
Southern Hybridization ◽  
Repeat Unit ◽  
Similar Species ◽  
Genomic Component ◽  
Library Hypothesis ◽  
Species Specific ◽  
Eukaryotic Organisms ◽  
Fish Signal

Abstract Satellite DNAis a genomic component present in virtually all eukaryotic organisms. The turnover of highly repetitive satellite DNAis an important element in genome organization and evolution in plants. Here we study the presence, physical distribution and abundance of the satellite DNAfamily AhTR1 in Anemone. Twenty-two Anemone accessions were analyzed by PCR to assess the presence of AhTR1, while fluorescence in situ hybridization and Southern hybridization were used to determine the abundance and genomic distribution of AhTR1. The AhTR1 repeat unit was PCR-amplified only in eight phylogenetically related European Anemone taxa of the Anemone section. FISH signal with AhTR1 probe was visible only in A. hortensis and A. pavonina, showing localization of AhTR1 in the regions of interstitial heterochromatin in both species. The absence of a FISH signal in the six other taxa as well as weak signal after Southern hybridization suggest that in these species AhTR1 family appears as relict sequences. Thus, the data presented here support the »library hypothesis« for AhTR1 satellite evolution in Anemone. Similar species-specific satellite DNAprofiles in A. hortensis and A. pavonina support the treatment of A. hortensis and A. pavonina as one species, i.e. A. hortensis s.l.

Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

2016 ◽  
Vol 97 (7) ◽  
pp. 1479-1482 ◽  
Author(s):  
Thomas J. Ashton ◽  
Meriem Kayoueche-Reeve ◽  
Andrew J. Blight ◽  
Jon Moore ◽  
David M. Paterson
Keyword(s):  
Dna Barcoding ◽  
Species Abundance ◽  
Microscopic Analysis ◽  
Cost Effective ◽  
Field Studies ◽  
Similar Species ◽  
Species Determination ◽  
Destructive Sampling ◽  
Species Specific ◽  
Non Destructive

Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.

scholarly journals Chikungunya: epidemiology

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 82 ◽  
Author(s):  
Lyle R. Petersen ◽  
Ann M. Powers
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Chikungunya Virus ◽  
Mosquito Species ◽  
Febrile Illness ◽  
Current Understanding ◽  
Global Public Health ◽  
Temperate Climates

Chikungunya virus is a mosquito-borne alphavirus that causes fever and debilitating joint pains in humans. Joint pains may last months or years. It is vectored primarily by the tropical and sub-tropical mosquito, Aedes aegypti, but is also found to be transmitted by Aedes albopictus, a mosquito species that can also be found in more temperate climates. In recent years, the virus has risen from relative obscurity to become a global public health menace affecting millions of persons throughout the tropical and sub-tropical world and, as such, has also become a frequent cause of travel-associated febrile illness. In this review, we discuss our current understanding of the biological and sociological underpinnings of its emergence and its future global outlook.

A new species of Ammothea and other pycnogonids from around Livingston Island, South Shetland Islands, Antarctica

2000 ◽  
Vol 12 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Tomás Munilla León
Keyword(s):  
New Species ◽  
Abundant Species ◽  
South Shetland Islands ◽  
Similar Species ◽  
Shetland Islands ◽  
Livingston Island ◽  
The Family ◽  
A New Species

Specimens of 22 species of pycnogonids belonging to twelve genera and seven families were collected during a cruise near Livingston Island, South Shetland Islands. Twenty two of the 94 stations yielded pycnogonids: the new species Ammothea hesperidensis is described fully, illustrated, and compared with similar species. The family Nymphonidae provided both the greatest diversity of species (seven) and number of specimens (35). The most abundant species were Achelia hoekii and Nymphon australe.

Grenadiers of the World Oceans: Biology, Stock Assessment, and Fisheries

2008 ◽  
Keyword(s):  
Stock Assessment ◽  
Distribution Patterns ◽  
Abundant Species ◽  
Trawl Survey ◽  
Bathymetric Range ◽  
Main Species ◽  
The World ◽  
Species Specific ◽  
Depth Ranges ◽  
Preanal Length

<em>Abstract</em>.—The objective of this study is to describe the distribution patterns of abundance and biomass, on a geographic and bathymetric basis, of the main macrourid species of Mozambique waters. Catch data from a demersal trawl survey (<EM>MOZAMBIQUE 07</EM>) were analyzed. The survey covered the continental shelf and upper-middle slope from 17°00’S to 26°50’S and from 100–700 m depth. Fourteen macrourid species were collected from 200 m and deeper. The most abundant species and the highest in biomass were <em>Coelorinchus braueri</em>, <em>C. trunovi, C. denticulatus</em>, <em>Ventrifossa nasuta</em>, and <em>Malacocephalus laevis</em>. Only those five species were analyzed in detail. The occurrence and yields by geographic and bathymetric range of these main species seem to reveal the existence of some species-specific preference for determinate depth ranges and/or geographic areas. Preanal length-weight relationships were estimated for <em>C. braueri, C. trunovi, </em>and <em>V. nasuta</em>: <em>a</em>= 0.00071; 0.00020; 0.00080; <em>b</em>= 2.50; 2.80; 2.76 and <em>r</em><sup>2</sup>= 0.93; 0.97; 0.78, respectively.

scholarly journals A Clinical Study on Chikungunya Fever in a Multidisciplinary Hospital of Dhaka City

2019 ◽  
Vol 37 (3) ◽  
pp. 124-129
Author(s):  
Safia Binte Rabbani ◽  
Pradip Ranjan Saha ◽  
Md Iqbal Hossain ◽  
Afsana Begum ◽  
Md Jahangir Talukder
Keyword(s):  
Public Health ◽  
Mosquito Species ◽  
Specific Treatment ◽  
Public Health Education ◽  
Adult Patients ◽  
Public Health Issue ◽  
Joint Involvement ◽  
Dhaka City ◽  
Antiinflammatory Drugs ◽  
Altered Liver

Chikungunya is one of the most rapidly spreading Aedes mosquito-borne viral infectious diseases. Recently in Bangladesh it has emerged as an important public health issue. Chikungunya virus (CHIKV) mostly spread by Aedes aegypti and Aedes albopictus, an anthropophilic mosquito species widely distributed in Asia, Europe, Africa and America. Our objective was to determine the clinical, biochemical and radiological features of patients at the acute phase of CHIKV infection. The purpose of study was to evaluate the literature and summarize the current state of CHIKV-associated disease, including clinical presentation, diagnosis, risk factors for development of severe diseases, complications and treatment. We present 253 confirmed cases of chikungunya having different clinical presentations occurring among adult patients from different background including foreigner in Dhaka city, admitted in a tertiary level hospital situated in Gulshan from march’17 to November 2017 . All patients had fever and joint pain. Other common features were rash, diarrhoea, vomiting, confusion, and altered liver biochemistry. Adult patients with multiple co-morbidities admitted in hospital with male preponderance of 59 % and rest were female 41%. Most common complication was post CHIKV arthritis (79%) and rest of the less common complications were post viral asthenia (34%), myocarditis (27%), pneumonitis (30%). Dengue was excluded in all patients. Paracetamol remained the mainstay of treatment during febrile periods, but around 62% of the patients had prolonged joint symptoms requiring non-steroidal antiinflammatory drugs, colchicines, steroid. Among joint involvement, ankle joints were commonly involved joint presented with post viral arthritis. Since there is no specific treatment of chikungunya, prevention through vector control and public health education is the key. J Bangladesh Coll Phys Surg 2019; 37(3): 124-129

scholarly journals Phylogeny of Anopheles (Kerteszia) (Diptera: Culicidae) Using Mitochondrial Genes

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 324 ◽  
Author(s):  
Karin Kirchgatter ◽  
Lilian de Oliveira Guimarães ◽  
Henrry Hugo Yañez Trujillano ◽  
Fernando Rafael Arias ◽  
Abraham Cáceres ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Species Recognition ◽  
Phylogenetic Analyses ◽  
Mosquito Species ◽  
Relevant Information ◽  
Neotropical Region ◽  
Similar Species ◽  
Serra Do Mar ◽  
Additional Tool ◽  
First Time

Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. Kerteszia mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of Kerteszia species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of Kerteszia species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from Anopheles (Kerteszia) boliviensis and Anopheles (Kerteszia) pholidotus. An. pholidotus sequences were recorded for the first time in Peru, whereas An. boliviensis sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as Anopheles (Kerteszia) cruzii clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming An. cruzii as a species complex, composed of at least three putative species.

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