Endophytic Bacterial Consortia as Biological Control of Bacterial Leaf Blight and Plant Growth Promoter of Rice (Oryza sativa L.)

2020 ◽  
Vol 4 (2) ◽  
pp. 134-145
Author(s):  
Zurai Resti ◽  
Yenny Liswarni ◽  
Martinius Martinius
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Bacillus Sp ◽  
Rice Seedlings ◽  
Bacterial Consortia ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

The consortia of endophytic bacteria with various mechanisms (competition, antibiotics, induction of resistance, and others), simultaneously, are more effective in controlling pathogens and increasing plant growth. The purpose of this study was to obtain endophytic bacterial consortia capable of suppressing of bacterial leaf blight and promoting the growth of rice plants. The study consisted of three experimental stages. The first stage was the test on the antibiosis ability of the endophytic bacterial consortia to suppress of pathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) using the Kirby Bauer method. The second stage was the test on the ability of endophytic bacterial consortia to promote the growth of rice seedlings. The experiment was arranged in a Completely Randomized Design (CRD) which consisted of six combinations of endophytic bacterial consortia and 15 replications within each combination. The endophytic bacterial consortia were introduced by soaking the seeds, and the observations were made on the plant height, number of leaves, root length, fresh weight, and dry weight of rice seedlings. Meanwhile, the third stage was the test on the ability of endophytic bacterial consortia to suppress of bacterial leaf blight (BLB) diseases and to promote the rice plant growth. The experiment was arranged in a Completely Randomized Design (CRD) with six combinations of endophytic bacterial consortia and five replications within each combination. The endophytic bacterial consortia were introduced by soaking the roots of the seedlings. The observations were made on the incidence of disease, disease severity, number of leaves, plant height, and number of tillers. The results showed that all endophytic bacterial consortia had antibiosis abilities. The best endophytic bacterial consortia for controlling bacterial leaf blight and promoting the growth of rice seedlings and plants were the C (Bacillus sp SJI; Bacillus sp HI) and D (Bacillus sp SJI; S.marcescens isolate JB1E3) consortia.

scholarly journals Pengaruh Pemberian Pupuk Organik Cair Nasa Dan Pupuk Kompos Terhadap Pertumbuhan Tanaman Kenaf ( Hibiscus cannabinus L)

2018 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Hairin Juanda ◽  
Tutik Nugrahini ◽  
Mahdalena Mahdalena
Keyword(s):  
Plant Growth ◽  
Leaf Area ◽  
Plant Height ◽  
Analysis Of Variance ◽  
Organic Fertilizer ◽  
Hibiscus Cannabinus ◽  
Stem Diameter ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

Effect of NASA Liquid Organic Fertilizer and Compost Fertilizer on Kenaf Plant Growth (Hibiscus cannabinus L). This study aims to determine the effect of NASA POC, compost fertilizer and the interaction of NASA POC and compost fertilizer on kenaf plants. This research was carried out at East Borneo Samarinda Indonesia. with a time of ± 4 months, starting from June to September 2016. The design of the study uses factorial completely randomized design (CRD) consisting of 2 treatments and 4 replications. The treatment consists of 2 factors. The first factor is the administration of NASA POC consisting of 4 levels, namely: j0: Control (without treatment), j1: POC with a concentration of 10 ml / liter of water, j2: POC with a concentration of 15 ml / liter of water, j3: POC with a concentration of 20 ml / liter of water. The second factor is Compost fertilizer consisting of 4 levels, namely: k0: Without the provision of Compost Fertilizer, k1: Composting 500 gram / polybag, k2: Composting Fertilizer 700 grams / polybag, k3: Composting 800 grams / polybag. From the analysis of variance showed that the effect of giving NASA POC (J), compost (K) and the interaction of NASA POC and compost (JK) did not affect the parameters of plant height 30 DAP, 60 DAP and 90 DAP, stem diameter 30 DAP, 60 DAP and 90 DAP, number of leaves 30 DAP, 60 DAP and 90 DAP, leaf area 30 DAP, 60 DAP and 90 DAP. 

scholarly journals The Effect of Goodplant and Hantu Plant Growth Regulator (ZPT) Applicationon the Growth and Production of Pakcoy Crop (Brassica rapa L.) Using Hidroponic Axis System

AGRIFOR ◽  
2019 ◽  
Vol 18 (1) ◽  
pp. 195
Author(s):  
Fauzi Irawan
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Plant Growth Regulator ◽  
Harvest Time ◽  
Land Preparation ◽  
Factor I ◽  
Randomized Design ◽  
Factor Ii ◽  
Number Of Leaves ◽  
Completely Randomized Design

The purpose of this research is to study the effect of Goodplant and ZPT Hantu concentration and their interaction on the growth and production of Pakcoy cropusing hydroponic axis system, and to obtain their proper concentrations for the best growth and production of Pakcoy crop.The study was conducted for three months from February to April 2017, from the time of seed preparation, land preparation until harvest time. The research site was conducted at Sumber Sari village, Barong Tongkok Subdistrict, Kutai Barat District. This study used a Completely Randomized Design (RAL), with 4 × 4 factorial experiment and 3 replications, consisting of 2 factors. Factor I is the concentration of Goodplant (N) consists of 4 levels, namely: no Goodplant application or control (n0), 5 ml 1-1 water (n1), 10 ml 1-1 water (n2), and 15 ml 1-1 water (n3). Factor II is the concentration of ZPT Hantu (H), consisting of 4 levels, ie: no ZPT Hantu application or control (h0), 1 ml 1-1 water (h1), 2 ml 1-1 water (h2), and 3 ml 1-1 water (h3).Goodplant treatment (N) affected very significantly on the plant height, leave number and weight of wetcrop. ZPT Hantu (H) treatment affected very significantly on the plant height, leave number and weight wetcrop.The interaction betweenGoodplant and ZPT Hantu(NxH) affected very significantlyon the plant height at 14 daysafter planting, plant height at 28 daysafter planting, number of leaves at 28 daysafter planting and weight of wetcrop; it affected significantly on the plant height at 21 daysafter planting; but it had no significant effect on the number of leaves at 14 days after planting and leave number at 21 days after planting.

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

scholarly journals UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI
Keyword(s):  
Plant Height ◽  
Growth Media ◽  
Growing Media ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

scholarly journals Response of Shallots of Batu Ijo Variety on Doses of N And K Fertilizers

Akta Agrosia ◽  
2021 ◽  
Vol 24 (1) ◽  
pp. 19-24
Author(s):  
Widodo Widodo ◽  
Marlin Marlin ◽  
Nancy B. Sitio
Keyword(s):  
Plant Growth ◽  
N Fertilizer ◽  
Tuber Weight ◽  
Plant Weight ◽  
Randomized Design ◽  
K Fertilizer ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Higher Doses

Shallots (Allium cepa var. Aggregatum L) have many varieties that can be grown in the highlands and lowlands and one of them is the Batu Ijo variety. Nutrients N and K play an important role for plant needs. This research was conducted from March to June 2019 in Medan Baru, Bengkulu City. This study used a completely randomized design with two factors. The first factor was the dose of N fertilizer and the second factor was the application of K fertilizer. The application of N fertilizer had a significant effect on the number of shallots at week 2. The Urea dose given decreases the number of spring onions. For the best treatment at plant height is the treatment of Urea 300 kg/ha and KCl 50 kg/ha. Plant growth from week 2 to 5 increased significantly. The application of K fertilizer has an effect on the number of shallots at week 2 and the number of tubers. The higher the plant age, the more the number of leaves produced, but at higher doses, the number of leaves and the number of tubers decreased. The best treatment for the number of leaves produced was Urea 300 kg/ha and without giving KCl. For the best treatment of fresh plant weight, namely Urea 300 kg/ha and KCl 150 kg/ha, the best treatment fresh tuber weight was Urea 300 kg/ha and KCl 150 kg/ha, for tuber diameter the best treatment was 200 kg/ha of urea and KCl 100 kg/ha and for the number of tubers of Urea 0 kg/ha and KCl 150 kg/ha.Keywords: shallots, N fertilizer, K fertilizer

scholarly journals ALTERNATIVE SUBSTRATES FOR PRODUCTION OF YELLOW PASSION FRUIT SEEDLINGS

2020 ◽  
Vol 7 (1) ◽  
pp. 43-48
Author(s):  
Eduardo Santana Aires ◽  
Carlos Alberto Aragão ◽  
Itala Laiane Silva Gomes ◽  
Gilmário Noberto de Souza ◽  
Isa Gabriela Vieira de Andrade
Keyword(s):  
Plant Height ◽  
Dry Matter ◽  
Passion Fruit ◽  
Dry Matter Accumulation ◽  
Alternative Substrates ◽  
Randomized Design ◽  
Length Width ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Yellow Passion Fruit

This study aimed to evaluate different substrates to produce yellow passion fruit seedlings. For this purpose, an experiment was carried out in a greenhouse, from April to June 2016. A completely randomized design with five replications, in a 6 x 5 factorial scheme was used. The first factor was the alternative substrates (poultry, cattle and equine manure, sugarcane and coconut bagasse, and Organomais compost). The second factor was the replacement levels to the commercial substrate of Plantmax® (100:0, 75:25, 50:50, 25:75, 0:100). The seedling evaluation was carried out 42 days after sowing. The following variables were analyzed: length, width, and the number of leaves; plant height and shoot dry matter. A significant effect (P0.05) for the interaction between the factors was verified. The addition of 60% cattle manure and Organomais to the commercial substrate Plantmax® promoted greater length, width, and the number of leaves, plant height, and shoot dry matter accumulation in the yellow passion fruit seedlings.

scholarly journals HOST RANGE PANTOEA ANANATIS THE CAUSAL AGENT OF BACTERIAL LEAF BLIGHT ON ALLIUM SPP.

2019 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Asrul Asrul ◽  
Umrah Umrah
Keyword(s):  
Host Range ◽  
Pathogenic Bacteria ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Pantoea Ananatis ◽  
Randomized Design ◽  
Spring Onion ◽  
Completely Randomized Design ◽  
Blight Disease ◽  
Range Test

Bacterial leaf blight disease caused by Pantoea ananatis has been reported affects some species of Allium spp. Here, we determined the species of Allium spp. that are not the hosts of the P.ananatis through a host range test. The study designed as a completely randomized design with five replications. Pathogenic bacteria of P. ananatis inoculated to shallot, onion, spring onion, garlic, chives, leek, and cung spring onion in the greenhouse of the Faculty of Agriculture Tadulako University. The incubation period and symptoms of after inoculated by P. ananatis then observed. The results showed that four llium species (onion, onion, scallion, and garlic) were hosts of                P. ananatis while the other three species (chives, leek, and cung spring onion) were not hosts of          P. ananatis.

scholarly journals Toleransi Plasma Nutfah Padi Lokal terhadap Salinitas

2018 ◽  
Vol 23 (1) ◽  
pp. 51 ◽  
Author(s):  
Tintin Suhartini ◽  
Try Zulchi
Keyword(s):  
Plant Height ◽  
Salinity Tolerance ◽  
Negative Correlation ◽  
Dry Weight ◽  
Gene Bank ◽  
Dead Leaf ◽  
Randomized Design ◽  
Highly Sensitive ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p><strong>Abstract</strong></p><p>The research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&amp;#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage &lt;50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage &lt;70%, and 82 accesions were sensitive to highly-sensitive.</p><p><strong>Abstrak</strong></p><p>Penelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &amp;plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati &lt;50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati &lt;70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.</p>

scholarly journals DETECTION AND TRANSMISSION OF RICE STUNT VIRUS ON CIHERANG AND SITU BAGENDIT VARIETIES

2019 ◽  
Vol 18 (2) ◽  
pp. 169
Author(s):  
Selvi Helina ◽  
Sri Sulandari ◽  
Sedyo Hartono ◽  
Andi Trisyono
Keyword(s):  
Plant Height ◽  
Incubation Period ◽  
Rice Production ◽  
Rice Plants ◽  
Inhibition Of Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Rice Ragged Stunt Virus

Detection and Transmission of  rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.

scholarly journals Effect of immersion duration of trichoderma harzianum on local chili growth

2019 ◽  
Vol 6 (3) ◽  
pp. 477-481
Author(s):  
Dini Puspita Yanty ◽  
Siti Hardianti Wahyuni
Keyword(s):  
Plant Height ◽  
Trichoderma Harzianum ◽  
Good Effect ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

Effect of immersion duration of trichoderma harzianum on local chili growth the aim of this researchis to see the old soaking ability of Trichoderma harzianum mushroom on the growth of local chili.The design used was a Completely Randomized Design (CRD) consisting of 6 treatments and 4replications, namely local chili seeds soaked with a 5-minute T.harzianum suspension, local chiliseeds soaked with a 10-minute T.harzianum suspension, local chili seeds that were soaked marinatedwith T.harzianum suspension for 15 minutes, local chili seeds soaked with T.harzianum suspensionfor 20 minutes, local chili seeds soaked with T.harzianum suspension for 25 minutes, Control (withoutimmersion with trichoderma harzianum suspension. Observed parameters are the percentage of local chiligrowth, plant height and number of leaves The results showed that the application of trichoderma harzianumhad a good effect on the growth of chili plants, soaking for 25 minutes was the best soaking on localchili seeds.

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