scholarly journals Mexican Sage (Salvia officinalis) Extraction Using Factorial Design and Its Effect on Chemical and Antibacterial Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
L. A. Conde-Hernández ◽  
M. L. Luna-Guevara ◽  
J. J. Luna-Guevara ◽  
J. Pérez-Vázquez ◽  
R. J. Aranda-García
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Factorial Design ◽  
Antibacterial Properties ◽  
Particle Morphology ◽  
Salvia Officinalis ◽  
Resistant Bacteria ◽  
Enterobacter Agglomerans ◽  
Pareto Chart ◽  
Pharmaceutical Industries

The essential oils (EOs) extracted by hydrodistillation (HYDRO) and steam distillation (SD) from Mexican Salvia officinalis L were analyzed for yield, chemical composition (GC-MS), particle morphology (SEM), antioxidant activity (ABTS), and antibacterial activity against Enterobacter agglomerans, Citrobacter freundii, Salmonella sp, E. coli, and Pseudomonas aeruginosa. The influence of the factors (method, quantity, and sample) was evaluated using a 23 full factorial design, Pareto chart, normal probability plot, main effects, and interaction plots in variance analysis on yield and antioxidant activity. The quantity, methods, sample, and the methods × sample and methods × quantity interactions were the most significant factors on yield (%). The sample, methods, and quantity × sample interaction were significant for antioxidant activity. EO yields were between 0.35 and 1.27 (% w/w), and the highest value was obtained by the HYDRO method using 50 g of whole sage leaves. The antioxidant activity values were in the range of 2.35 to 3.44 mg Trolox equivalent/g. Camphor, limonene, camphene, and caryophyllene were the main compounds identified. Micrographs of sage leaves showed relevant changes in the structure after extraction. The antibacterial activity was confirmed with the inhibition diameter and inhibition percentage of all bacteria, and P. aeruginosa was the most resistant bacteria. Finally, S. officinalis EO potentials can be considered an alternative natural preservative for the food and pharmaceutical industries.

IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Pradeep K Naik ◽  
Debasmita Dubey ◽  
Priya Cheruvanachari ◽  
Animesh Pattnaik ◽  
Swaraj K Babu
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Dpph Method ◽  
Celastrus Paniculatus

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality. Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method. Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity. Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens. Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity

scholarly journals Antibacterial Activity of Moroccan Zantaz Honey and the Influence of Its Physicochemical Parameters Using Chemometric Tools

2021 ◽  
Vol 11 (10) ◽  
pp. 4675
Author(s):  
Youssef Elamine ◽  
Hamada Imtara ◽  
Maria Graça Miguel ◽  
Ofélia Anjos ◽  
Letícia M. Estevinho ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Principal Component ◽  
Physicochemical Characteristics ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Coumaric Acid ◽  
Atlas Mountains ◽  
Methyl Syringate ◽  
Chemometric Tools

The emergence of multidrug-resistant bacteria has prompted the development of alternative therapies, including the use of natural products with antibacterial properties. The antibacterial properties of Zantaz honey produced in the Moroccan Atlas Mountains against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was evaluated and analyzed using chemometric tools. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) against S. aureus were the lowest (112.5 ± 54.5 mg/mL), revealing that this species was most sensitive to Zantaz honey. P. aeruginosa showed an intermediate sensitivity (MIC= 118.75 ± 51.9 mg/mL), while E. coli was the most resistant to treatment (MIC = 175 ± 61.2 mg/mL). Content of monosaccharides, certain minerals, and phenolic compounds correlated with antibacterial activity (p < 0.05). Principal component analysis of physicochemical characteristics and antibacterial activity indicated that the parameters most associated with antibacterial activity were color, acidity, and content of melanoidins, fructose, epicatechin, methyl syringate, 4-coumaric acid, and 3-coumaric acid.

scholarly journals Evaluation of Antioxidant and Antibacterial Properties of Magnolia champaca L (Magnoliaceae) Stem Bark Extract

2020 ◽  
Vol 23 (2) ◽  
pp. 96-102
Author(s):  
Md Mahadi Hasan ◽  
Masuma Akter ◽  
Md Ekramul Islam ◽  
Md Aziz Abdur Rahman ◽  
Mst Shahnaj Parvin
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Stem Bark ◽  
Total Antioxidant ◽  
Pharmacologically Active

The current study was designated to explore the antioxidant and antibacterial properties of the methanolic extract of Magnolia champaca stem bark and its different fractions. Antioxidant activity was assessed using total antioxidant capacity, ferric reducing power, DPPH, hydroxyl and hydrogen peroxide scavenging assay. Antibacterial activity was evaluated against five gram positive and five gram negative bacteria using disc diffusion assay method. Among the different fractions, chloroform fraction (CHF) and ethyl acetate fraction (EAF) showed the highest antioxidant activity whereas aqueous fraction (AQF) showed lowest activity in DPPH radical scavenging assay with IC50 of 12.12, 22.41 and 55.16 μg/ml, respectively. Both of the extracts CHF and EAF also exhibited highest total antioxidant capacity, ferric reducing power and hydrogen peroxide scavenging activity with concentration dependent manner when compared to standard BHT. Moderate to potent antibacterial activity was observed against all tested organisms compared to standard azithromycin. The results from the present study revealed that the different fractions of stem bark of M. champaca specially CHF and EAF possess antioxidant and antibacterial property which support its use in traditional medicine and suggesting that the plant may be further investigated to discover its pharmacologically active natural products. Bangladesh Pharmaceutical Journal 23(2): 96-102, 2020

scholarly journals Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial ActivityIn Vivo

2013 ◽  
Vol 57 (10) ◽  
pp. 4945-4955 ◽  
Author(s):  
Divya Prakash Gnanadhas ◽  
Midhun Ben Thomas ◽  
Rony Thomas ◽  
Ashok M. Raichur ◽  
Dipshikha Chakravortty
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Serum Albumin ◽  
Serum Proteins ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Human Society ◽  
Vis Spectroscopy

ABSTRACTThe emergence of multidrug-resistant bacteria is a global threat for human society. There exist recorded data that silver was used as an antimicrobial agent by the ancient Greeks and Romans during the 8th century. Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities, with minimal cytotoxic effects on the cells. However, very few reports have shown the usage of AgNPs for antibacterial therapyin vivo. In this study, we deciphered the importance of the chosen methods for synthesis and capping of AgNPs for their improved activityin vivo. The interaction of AgNPs with serum albumin has a significant effect on their antibacterial activity. It was observed that uncapped AgNPs exhibited no antibacterial activity in the presence of serum proteins, due to the interaction with bovine serum albumin (BSA), which was confirmed by UV-Vis spectroscopy. However, capped AgNPs [with citrate or poly(vinylpyrrolidone)] exhibited antibacterial properties due to minimized interactions with serum proteins. The damage in the bacterial membrane was assessed by flow cytometry, which also showed that only capped AgNPs exhibited antibacterial properties, even in the presence of BSA. In order to understand thein vivorelevance of the antibacterial activities of different AgNPs, a murine salmonellosis model was used. It was conclusively proved that AgNPs capped with citrate or PVP exhibited significant antibacterial activitiesin vivoagainstSalmonellainfection compared to uncapped AgNPs. These results clearly demonstrate the importance of capping agents and the synthesis method for AgNPs in their use as antimicrobial agents for therapeutic purposes.

scholarly journals Correlation of Total Polyphenolic Content with Antioxidant Activity of Hydromethanolic Extract and Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Zakaria Khiya ◽  
Yassine Oualcadi ◽  
Abderrahmane Gamar ◽  
Fatima Berrekhis ◽  
Touria Zair ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Salvia Officinalis ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Pharmaceutical Industries

The purpose of this study is to determine the total content of phenols, flavonoids, and condensed tannins, as well as on the antioxidant activity of the extract, and their fractions were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), phosphomolybdate reduction (or total antioxidant capacity), and cyclic voltammetry (CV). The hydromethanolic extract of Salvia officinalis showed the highest values of total phenolic (176 mgGAE/g of extract) and condensed tannins (162.53 mgEC/g of extract) from the Boulemane and Khenifra regions, respectively. The results showed that the best DPPH assay was found in the ethyl acetate fraction of Salvia officinalis leaves of the Boulemane region (IC50 = 0.002 mg/ml). For the ethyl acetate and butanolic fractions of Salvia officinalis leaves, those collected from different regions have a better reducing capacity (EC50 = 0.021 mg/ml, respectively). For the total antioxidant capacity, the best activity was found in the aqueous fraction of Salvia officinalis leaves of the Boulemane region (108 mgGAE/g of extract). By the cyclic voltammetry method, hydromethanolic extract of Salvia officinalis leaves from the Boulemane region showed an important result (288.8 mgGAE/g). There was a positive correlation between total phenol content (TPC), condensed tannin content (TCT), and total antioxidant capacity (TAC) (r = 0.932, r = 0.896, respectively). The main compounds that have been identified in the hydromethanolic extract of Salvia officinalis are ascorbic acid, gallic acid, 4-hydroxybenzoic acid, tannic acid, and rutin. Due to their antioxidant property, the leaf extracts from Salvia officinalis are used as natural preservative ingredients in food and/or pharmaceutical industries.

scholarly journals The Lyophilization Process Maintains the Chemical and Biological Characteristics of Royal Jelly

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Andresa Piacezzi Nascimento ◽  
Larissa Ariana Roveroni Moraes ◽  
Nathália Ursoli Ferreira ◽  
Gabriela de Padua Moreno ◽  
Fernanda Grassi Mangolini Uahib ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Royal Jelly ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Resistant Bacteria ◽  
Bioactive Substances ◽  
Antibiotic Resistant ◽  
Chromatographic Profile ◽  
Main Fatty Acid

The alternative use of natural products, like royal jelly (RJ), may be an important tool for the treatment of infections caused by antibiotic-resistant bacteria. RJ presents a large number of bioactive substances, including antimicrobial compounds. In this study, we carried out the chemical characterization of fresh and lyophilized RJ and investigated their antibacterial effects with the purpose of evaluating if the lyophilization process maintains the chemical and antibacterial properties of RJ. Furthermore, we evaluated the antibacterial efficacy of the main fatty acid found in RJ, the 10-hydroxy-2-decenoic acid (10H2DA). Chromatographic profile of the RJ samples showed similar fingerprints and the presence of 10H2DA in both samples. Furthermore, fresh and lyophilized RJ were effective against all bacteria evaluated; that is, the lyophilization process maintains the antibacterial activity of RJ and the chemical field of 10H2DA. The fatty acid 10H2DA exhibited a good antibacterial activity againstStreptococcus pneumoniae. Therefore, it may be used as an alternative and complementary treatment for infections caused by antibiotic-resistantS. pneumoniae.

scholarly journals Proximate Analysis, Antioxidant Activity, and Antibacterial Activity of Fish Sausages Fortified with Bee Bread Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Salma Malihah Mohammad ◽  
Amirun Afiq Badrul Hisham ◽  
Nur Atifa Mustapa ◽  
Kim Wei Chan ◽  
Norhasnida Zawawi
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Antibacterial Properties ◽  
Frozen Storage ◽  
Negative Control ◽  
Plate Count ◽  
Oxidation Activity ◽  
Bee Bread

Bee bread is rich in phenolic compounds and recently has gained attention as a food additive, which provides an alternative use to synthetic preservatives. Therefore, this study aims to analyze the proximate composition and antioxidant and antibacterial activities of fish sausages fortified with Heterotrigona itama bee bread ethanolic (BBE) extract at 0.25, 0.5, and 0.75% concentrations. Incorporation of BBE in fish sausages significantly increased the carbohydrate level and lowered the moisture content. The highest phenolic and flavonoid content were observed in fish sausages with 0.75% BBE with 23.46 ± 1.60 mg GAE/g sample and 8.05 ± 0.24 mg rutin/g sample, respectively. The antioxidant activity revealed the highest DPPH scavenging activity for 0.75% BBE fish sausage compared to synthetic additive BHT. After 28 days of frozen storage, lipid oxidation activity of fish sausages with 0.75% BBE had lower thiobarbituric acid reactive substances (TBARS) value than the negative control, but similar ( p > 0.05 ) to BHT. The phenolic compounds and antioxidant activities were significantly reduced ( p < 0.05 ) after 28 days of storage. Based on the antibacterial activity, BBE was able to inhibit tested foodborne pathogens, and the addition of BBE in fish sausage showed total plate count below 6 log10 CFU/g within 6 days of frozen storage. This research demonstrated the BBE efficiency as a natural antioxidant with antibacterial properties in fish sausages.

scholarly journals Discovery of Antibacterial Dietary Spices That Target Antibiotic-Resistant Bacteria

2019 ◽  
Vol 7 (6) ◽  
pp. 157 ◽  
Author(s):  
Dan Zhang ◽  
Ren-You Gan ◽  
Arakkaveettil Kabeer Farha ◽  
Gowoon Kim ◽  
Qiong-Qiong Yang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Antioxidant Activities ◽  
Antibacterial Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Dietary Spices

Although spice extracts are well known to exhibit antibacterial properties, there is lack of a comprehensive evaluation of the antibacterial effect of spices against antibiotic-resistant bacteria. In the present study, ethanolic extracts from a total of 67 spices were comprehensively investigated for their in vitro antibacterial activities by agar well diffusion against two common food-borne bacteria, Staphylococcus aureus and Salmonella enteritidis, with multi-drug resistance. Results showed that S. aureus was generally more sensitive to spice extracts than S. enteritidis. Of the 67 spice extracts, 38 exhibited antibacterial activity against drug-resistant S. aureus, while only four samples were effective on drug-resistant S. enteritidis. In addition, 11 spice extracts with inhibition zones greater than 15 mm were further verified for their broad-spectrum antibacterial properties using another 10 drug-resistant S. aureus strains. It was found that five spice extracts, including galangal, fructus galangae, cinnamon, yellow mustard seed, and rosemary, exhibited the highest antibacterial capacity. Further cytotoxicity of these 11 spices was determined and LC50 values were found to be more than 100 μg/mL except for galangal, rosemary, and sage, whose LC50 values were 9.32 ± 0.83, 19.77 ± 2.17, and 50.54 ± 2.57, respectively. Moreover, the antioxidant activities (ferric-reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) values) and total phenolic content (TPC) of spice extracts were determined to establish possible correlations with the antibacterial activity. Although the antibacterial effect was positively correlated with the antioxidant activities and TPC, the correlation was weak (r < 0.5), indicating that the antibacterial activity could also be attributed to other components besides antioxidant polyphenols in the tested spice extracts. In conclusion, dietary spices are good natural sources of antibacterial agents to fight against antibiotic-resistant bacteria, with potential applications as natural food preservatives and natural alternatives to antibiotics in animal feeding.

scholarly journals PhSeZnCl in the Synthesis of Steroidal β-Hydroxy-Phenylselenides Having Antibacterial Activity

2019 ◽  
Vol 20 (9) ◽  
pp. 2121 ◽  
Author(s):  
Jastrzebska ◽  
Mellea ◽  
Salerno ◽  
Grzes ◽  
Siergiejczyk ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Biofilm Formation ◽  
Ring Opening ◽  
Antibacterial Properties ◽  
Multidrug Resistant ◽  
Bacterial Biofilm ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Ring Opening Reaction

We report here the reaction of in situ prepared PhSeZnCl with steroid derivatives having an epoxide as an electrophilic functionalization. The corresponding ring-opening reaction resulted to be regio- and stereoselective affording to novel phenylselenium-substituted steroids. Assessment of their antibacterial properties against multidrug-resistant bacteria, such as Pseudomonas aeruginosa Xen 5 strain, indicates an interesting bactericidal activity and their ability to prevent bacterial biofilm formation.

scholarly journals Phenolic Compounds in Extracts of Hibiscus acetosella (Cranberry Hibiscus) and Their Antioxidant and Antibacterial Properties

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4190 ◽  
Author(s):  
Jae Il Lyu ◽  
Jaihyunk Ryu ◽  
Chang Hyun Jin ◽  
Dong-Gun Kim ◽  
Jung Min Kim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Antibacterial Properties ◽  
Activity Level ◽  
Leaf Extracts ◽  
Abts Assay ◽  
Activity Group ◽  
Zones Of Inhibition ◽  
Low Activity

Hibiscus species are rich in phenolic compounds and have been traditionally used for improving human health through their bioactive activities. The present study investigated the phenolic compounds of leaf extracts from 18 different H. acetosella accessions and evaluated their biofunctional properties, focusing on antioxidant and antibacterial activity. The most abundant phenolic compound in H. acetosella was caffeic acid, with levels ranging from 14.95 to 42.93 mg/100 g. The antioxidant activity measured by the ABTS assay allowed the accessions to be classified into two groups: a high activity group with red leaf varieties (74.71–84.02%) and a relatively low activity group with green leaf varieties (57.47–65.94%). The antioxidant activity was significantly correlated with TAC (0.933), Dp3-Sam (0.932), Dp3-Glu (0.924), and Cy3-Sam (0.913) contents (p < 0.001). The H. acetosella phenolic extracts exhibited antibacterial activity against two bacteria, with zones of inhibition between 12.00 and 13.67 mm (Staphylococcus aureus), and 10.67 and 13.33 mm (Pseudomonas aeruginosa). All accessions exhibited a basal antibacterial activity level (12 mm) against the Gram-positive S. aureus, with PI500758 and PI500764 exhibiting increased antibacterial activity (13.67 mm), but they exhibited a more dynamic antibacterial activity level against the Gram-negative P. aeruginosa.

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