scholarly journals Isolation And Test Potential Of Phosphate Solubilization Microorganisms On Andisols Sinabung Eruption Impact On Some Thickness Of Ash In Karo District

1970 ◽  
Vol 5 (3) ◽  
pp. 328-339
Author(s):  
Nofriyanto Pakolo ◽  
Mariani Sembiring ◽  
Abdul Rauf

Andisol soil erupted ash sinabung has a thickness level, where each thickness has microbial activity and nutrient status of soil who different. This study aims to determine the potential of phosphate solubilization microorganisms on soil erupted ash sinabung. This research was conducted at Soil Biology Laboratory of Faculty of Agriculture USU, which the isolation of microorganisms is fungi and bacteria from the soil erupted Sinabung in some ash thickness to get the isolates to be tested the potential solubility. Isolate tested for their solubility potential in two ways: solid pikovskaya medium with source of phosphate Ca3(PO4)2, AlPO4, FePO4, and RP (Rock Phosphate) and in Andisol soil incubation with microbial dose as much as 1 ml. The result showed that there were 6 isolates of fungi (J1-J6) and 6 bacterial isolates (B1-B6) and the provision of phosphate solubilization microorganisms on Andisol soil has different potential in increasing microbial population, soil respiration, P-total soil, P-available soil, but can decrease C-organic soil with soil acidity degree (pH) and each type of MPF isolate tested will be able to dissolve P-not available to be P-available on solid pikovskaya medium with source of phosphate Ca3(PO4)2, AlPO4, FePO4 and Rock Phosphate but have different capabilities

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
IKHWANI IKHWANI ◽  
NONON SARIBANON ◽  
TATANG MITRA SETIA ◽  
ERNY YUNIARTI ◽  
Jumakir Jumakir

Abstract. Ikhwani, Saribanon N, Setia TM, Yuniarti E, Jumakir. 2021. Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field. Biodiversitas 22: 4722-4730. This study aims to isolate, select and identify molecular characterization of bacteria from alluvial and latosol soil paddy field. This study has been conducted from February-June 2020 in the Soil Biology Laboratory, ICALRRD, Bogor. Alluvial and latosol soil samples were selected from Pusakanagara and Muara experimental gardens, Muara district. The result showed that bacteria growth in N-free media was higher from latosol soil as compared to alluvial soil. The higher nitrogenase activity isolates, could form ethylene gas concentration 1.1.E + 09 µmol/mL/hours, 1.7.E + 09 µmol/mL/hours and 1.3.E + 09 µmol/mL/hours from  alluvial 6, latosol 4, and latosol 9.  Three isolates have great PSB performances, i.e. isolates No. 4.2, 2.1 and 2 could dissolve 14.398 mg l-1, 12.648 mg l-1 and 12.145 mg l-1 of phosphate, from latosol soil. The highest N-fixing and solubilization capacity identified by molecular characterization i.e latosol 4, 4.2 and alluvial 7.1, alluvial-1.11. The phylogeny analyzed the presence of bacterial isolates in two distinct groups. Latosol 3.1 and latosol 4.2 isolates clustered in the same as the genus Lysinibacillus bacteria. The Alluvial 7.1 bacterial isolate showed similarities by Fictibacillus sp. and alluvial 1.2 isolate showed similarities by the genus Bacillus sp.


2021 ◽  
Vol 8 (2) ◽  
pp. 415-426
Author(s):  
Wahyu Satria Eginarta ◽  
Yulia Nuraini ◽  
Jati Purwani

Rice is an important commodity in human life where its production increase must be followed by environmental sustainability and land health. The achievement of increased rice production supported by environmental sustainability can be achieved by utilizing biological fertilizers. One of the microbes that can be used as nutrient providers for rice plants is cyanobacteria. This study was carried out in a greenhouse and soil biology laboratory of the Indonesian Soil Research Institute using a randomized block design with four replications. The treatments tested consisted of F0 (without treatment), F1 (wet cyanobacteria isolate), F2 (dry cyanobacteria isolate), F3 (cyanobacteria + rock phosphate), F4 (cyanobacteria + biochar), F5 (cyanobacteria + kaolin), F6 (cyanobacteria + rock phosphate + kaolin), F7 (cyanobacteria + rock phosphate + biochar), F8 (cyanobacteria + kaolin + biochar). The results showed that the cyanobacteria formula treatment with biochar (F4) carriers showed the highest total population in the parameters of the cyanobacteria population, bacteria, and fungi so that the interaction was said to be positive. The application of cyanobacteria formula to soil chemistry did not significantly affect the post-harvest soil N, P, and K, while the increase in soil pH occurred in the overall treatment of the cyanobacteria formula. Treatment of cyanobacteria formula with biochar carrier (F4) had significantly different results on parameters of number of tillers, number of panicles, grain weight, number of grains.


Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.


Author(s):  
Márcia H. Beck ◽  
Pedro A. V. Escosteguy ◽  
Deborah P. Dick

ABSTRACT The effect of humic acids (HA) on phosphorus (P) availability is still contradictory; thus, it is necessary to identify the conditions that play a crucial role in this effect. The aim of this study was to investigate the effect of HA application, combined with doses of P, on the content of this nutrient in a Latosol with and without acidity correction. Two experiments were carried out, one with HA from peat and another with HA from mineral charcoal (leonardite). Doses of these acids (0; 1.12 and 5.62 mg C g-1 of soil) and P (26.2 and 104.7 mg P g-1 of soil, 1 and 4-fold higher than recommended, respectively) were tested at soil pH 4.5 and 7.0, in a three-factorial design. The soil was incubated for 20 days and the soil-P content was measured by Mehlich-1 and remaining-P tests. The effect of HAs on P availability varied with the P doses and soil acidity. Humic acids application increases P content in Latosol when P dose is higher than recommended and there is no acidity correction (pH 4.5). However, there is no effect of HAs application on soil-P content when applying the recommended amount of this nutrient, irrespective of the pH value.


2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.


Author(s):  
Giuseppe Haydn Lacorte ◽  
Remedios S. Flamiano ◽  
Malona V. Alinsug ◽  
Paolo M. Tagaloguin

Rhizospheric bacteria (PGPR) associated with 5 banana cultivars planted in SOCCSKSARGEN were studied. Microbial population in newly established and old plantation was compared. Bacteria were purified and their beneficial characteristics were determined. This study was able to collect one hundred twenty (120) bacterial isolates, 20 (17%) of which were nitrogen-fixers, nineteen (19/20) were phosphate solubilizers and seventeen (17/20) were IAA producers. From this study, five bacterial isolates coded PE05, RS10, PE11, PE13, and PE18 were found positive for nitrogen fixation, phosphate solubilization and IAA production which may be further evaluated for their potential application in the formulation of biofertilizers for banana cultivation. Read full article here.


2014 ◽  
Vol 1 (2) ◽  
pp. 81
Author(s):  
Lutfi Izhar ◽  
Anas D. Susila

<p>ABSTRACT</p><p><br />Vegetables are important agricultural commodities. Productivity of vegetables in Indonesia is still low. One effort that can be done is an application of specific fertilizer recommendations. Fertilizer recommendation based on soil analysis is still rarely for vegetable crops and need further development. The purpose of this paper is to describe some fertilizer recommendations based on soil analysis for vegetable crops. Three stages to consider in the assessment of the research such as soil incubation, correlation test, calibration test and fertilizer <br />recommendation statue. Application all this stages of soil method recommendation in Indonesia is still not widely applied. Two researches which were completed until the entire stage has been done for yard long beans and <br />tomatoes. Recommendations for tomatoes on Inceptisols soil type with very low nutrient status of soil K was 180 kg K2O ha- 1, a low K soil nutrient status was 131.4 kg K2O ha-1, soil K nutrient status was 82.2 kg K 2O ha-1. Yard <br />long bean that planted on Ultisol soil type with low soil P nutrient status was recommended by an application of 185.8 kg P2O5 ha-1, medium soil P nutrient status was added 174.9 kg P2O5 ha-1. Development of fertilizer recommendation based on soil testing to support agricultural development in Indonesia still has some problems and need some strategies for further research, application and dissemination in the future.</p><p>Key words: vegetables, soil test, fertilizer recommendation</p>


2021 ◽  
Author(s):  
Kehinde Oluwadamilare Sowunmi

Abstract A study investigated impact of cement dust pollution from Ewekoro cement industry on soil microbes. pH of the soil ranged from 6.27±0.03- 6.47 and soil moisture content ranged from 15.78±2.52- 9.65±1.16. The levels of heavy metals except Mg, Zn and Na were higher within the factory than in the control. Microbial population diversity increased steadily away from the factory and this variation could be attributed to the impact of pH and heavy metals on microbial population. The lower counts of bacteria compared to fungi may be as a result of the nutrient status of the soil and the bacteria counts in polluted soil were lower than the fungal counts in control soil. The bacteria and fungi was influenced by the cement dust deposition. The study was published in the journal ‘Phenomenon: Microbes and the Cement Industry’.


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