Antibacterial Activities Test of Combination of Ethanolic Extract of Betel Leaves (Piper betle L.) and Basil Leaves (Ocimum basilicum L.) Against Staphylococcus aureus

Betel leaf extract and basil leaf extract contain phenol compounds, safonin, flavonoids, tannins, and essential oils that act as antibacterials. Skin is very susceptible to infection or other skin diseases one of which is caused by Staphylococcus aureus bacteria. This research needs to be done to test the antibacterial activity of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The process of extraction of betel leaf and basil leaves was done with 96% ethanol solvent using maceration method. The combinations used in the sample solution were the concentration of ethanol extract of green betel leaf (0%, 15%, 30%, 45%, 60%, 75%) and basil leaf extract (0%, 15%, 30%, 45%, 60 %, 75%). Furthermore, the antibacterial activity was tested for S. aureus with well diffusion method. The data obtained were tested statistically using Anova and Duncan's advanced test. The result of statistical analysis showed difference of drag zone (p <0,05) between various concentration of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The smallest resin zone of ethanol extract of betel leaf and ethanol extract of basil leaves is at 0% concentration (S0 and K0) and highest at 75% concentration (S5 and K5). While the smallest zone of inhibition for the combination of ethanol extract of betel leaf and ethanol extract of basil leaves at concentration S0K0, S0K1 and S0K2 is 0 mm and highest at S5K5 concentration is 31,3 mm. The higher concentration of betel leaf extract, basil leaf extract and the combination of the two extracts, the inhibition of diameter zone will be better. It can be concluded that the combination of betel leaf extract and basil leaf extract has the ability to inhibit the growth of S. aureus better than single extract.

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The Effectiveness Test of Betel Leaf Ethanol Extract Cream (Piper Betle Linn) Toward Propionibacterium acnes Bacterial Growth

BioScientia Medicina ◽

10.32539/bsm.v4i2.112 ◽

2020 ◽

Vol 4 (2) ◽

pp. 10-17

Author(s):

Resva Meinisasti ◽

Zamharira Muslim ◽

Krisyanella ◽

Raden Sunita

Keyword(s):

Antibacterial Activity ◽

Active Substance ◽

Propionibacterium Acnes ◽

Skin Diseases ◽

Acne Vulgaris ◽

Ethanol Extract ◽

Diffusion Method ◽

Piper Betle ◽

Betel Leaf ◽

Acne Treatment

Background: One of skin diseases that is frequently taking attention among teenagers and young adults is acne or in medical term called acne vulgaris. Acne treatment can be treated by repairing follicular abnormalities, decreasing sebum production, decreasing the number of Propionibacterium acnes colonies and reducing inflammation of the skin. The bacterial population of Propionibacterium acnes can be reduced by giving an antibacterial substance such as erythromycin, clindamycin and benzoyl peroxide. In the treatment of acne we can get antibacterial originating from nature, one of which can be obtained in the secondary metabolism of plants. The extracts and essential oils of betel leaf contain antibacterial and antifungal activities. The effectiveness of the use of betel leaf ethanol extract (Piper betle Linn) in acne treatment can be improved by creating formulations in form of cream preparations. Formulations in cream preparations will affect the amount and speed of active substances that can be absorbed. Objectives: To determine the effectiveness of the ethanol extract cream of betel leaf (piper betle linn) cream in formulas with what percentage of active substance has the most-inhibitory effect on the growth of Propionibacterium acnes. Methods:The study used experimental research. Propionibacterium acnes samples were diluted in 0.9% physiological NaCl sterile and embedded in Nutrient Agar (NA) media. The media was inserted into an incubator at 37 Â° C for 24 hours. The test of antibacterial activity used the disk diffusion method. The antibacterial activity test results were statistically analyzed using the Statistical Product Services Solution (SPSS 17) program with a confidence level of 95% (Î± = 0.05). Results: The three creams containing betel leaf ethanol extracts at percentages of 5%, 10% and 15% for each had inhibitory zones: 9.8 mm, 15.85 mm, 17.35 mm. Conclusion: Cream that contains 15% active substance has the strongest inhibition.

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Antibacterial activities of combination of ethanol extract of Delima fruit (Punica granatum L.) and Sereh stem (Cymbopogon citratus) on Staphylococcus aureus

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20195442 ◽

2019 ◽

Vol 6 (12) ◽

pp. 5037

Author(s):

Melia Pebrina ◽

Indah Komala Sari ◽

Eliza Arman ◽

Honesty Diana Morika

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Punica Granatum ◽

Antibacterial Activities ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Randomized Design ◽

Pomegranate Peel Extract

ABSTRACT Background: Pomegranates contain chemical saponins and flavonoids while lemongrass stems have flavonoid chemical compounds. Substances that can inhibit the linking of bacteria are saponins and flavonoids. The most common microorganism found in ulcus diabetikum (ulcer diabetes) is Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of a combination of pomegranate ethanol extract and ethanol extract of lemongrass stems against S. aureus bacteria.Methods: This study used an experimental design with a complete randomized design study divided into 5 groups: groups 1 (75:25), 2 (50:50), 3 (25:75), positive control (tetracycline) and negative control. Manufacture of pomegranate peel extract and lemongrass stems was done by maceration method for further rotary, after the extract was obtained tested for antibacterial activity by diffusion method using a cylinder. With the test used analysis of variance one way.Results: The results of this study showed that the combination of pomegranate ethanol extract and citronella stem ethanol extract showed activity against S. aureus with a ratio of 15 ul:5 ul, 15 ul:15 ul, 5 ul:15 ul with inhibitory diameter of respectively 13 mm, 11 mm, 8 mm. Whereas for positive control with chloramphenicol, the inhibition area is 9 mm.Conclusions: Statistically the combination of pomegranate ethanol extract and lemongrass stems has antibacterial power which uses p=0.005 and p<0.05. The combination of pomegranate ethanol extract and lemongrass stems has very strong antibacterial activity against S. aureus due to inhibition zones of 10-20 mm.

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SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

Journal of Pharmacopolium ◽

10.36465/jop.v1i1.394 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Vera Nurviana

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Diffusion Method ◽

Water Fraction ◽

Tetracycline Hcl

Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl. Keywords: Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.

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Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

Talenta Conference Series Science and Technology (ST) ◽

10.32734/st.v1i2.290 ◽

2018 ◽

Vol 1 (2) ◽

pp. 135-143 ◽

Cited By ~ 1

Author(s):

Dwi Latifah Sari ◽

Sudarmi Sudarmi ◽

Popi Patilaya

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Leaf Extract ◽

Skin Diseases ◽

Ethanol Extract ◽

Positive Control ◽

Annona Muricata ◽

Phytochemical Screening ◽

Activity Test ◽

Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

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Aktivitas antibakteri salep ekstrak etanol daun sirih hijau (Piper betleL.) Terhadap infeksi bakteri Staphylococcus aureus

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v5i2.129 ◽

2018 ◽

Vol 5 (2) ◽

pp. 56

Author(s):

Rissa Laila Vifta ◽

Muhammad Andri Wansyah ◽

Anita Kumala Hati

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Ethanol Extract ◽

Positive Control ◽

Piper Betle ◽

In Vitro Test ◽

In Vivo Test ◽

Betel Leaf

Abstrak Sirih hijau (Piper betle L.) adalah tanaman yang mudah dijumpai di Indonesia. Secara empiris, sirih hijau digunakan sebagai antibakteri pada luka. Penelitian ini bertujuan untuk mengetahui aktivitas salep ekstrak etanol daun sirih hijau sebagai antibakteri. Pengujian efek antibakteri dilakukan secara in vitro dan in vivo. Ekstrak etanol daun sirih hijau dibuat dengan cara maserasi. Pengujian secara in vitro dilakukan menggunakan metode mikrodilusi terhadap Staphylococcus aureus untuk mengetahui nilai konsentrasi hambat minimum ekstrak daun sirih hijau pada konsentrasi 3, 4, dan 5%. Kemudian ekstrak dibuat salep dan diuji secara in vivo menggunakan hewan uji tikus yang terdiri dari kelompok kontrol negatif, kelompok ekstrak (konsentrasi 3,4, dan 5%), serata pembanding kontrol positif gentamisin 0,1%. Hasil uji dianalisis menggunakan Sapiro-Wilk dan dilanjutkan dengan uji ANAVA serta uji LSD untuk mengetahui perbandingan hasil kelima kelompok perlakuan. Hasil uji in vitro menunjukkan bahwa konsentrasi hambat minimum ekstrak daun sirih yang optimal adalah 5%. Hasil uji in vivo menunjukkan lama kesembuhan luka meningkat seiring dengan bertambahnya konsentrasi ekstrak daun sirih hijau. Hasil uji normalitas, Anava, dan uji LSD pada uji in vivo memberikan hasil yang sejalan. Konsentrasi 4 dan 5% merupakan konsentrasi efektif dengan aktivitas waktu kesembuhan luka tidak berbeda bermakna dengan kontrol positif (gentamisin 0,1%) dengan rerata waktu berturut-turut 6,20±0,80 dan 6,00±0,71 hari. Dapat disimpulkan bahwa hasil uji antibakteri pada salep sejalan dengan uji secara in-vitro ekstrak daun Sirih Hijau yang memiliki potensi sebagai antibakteri.Kata kunci: Daun sirih hijau, Antibakteri, Salep, Luka Antibacterial activity from ointment contains extract of green betle leaves (Piper betle l.) to Staphylococcus aureus bacterial infection Abstract Green betel (Piper betle L.) is a plant that is easy to find in Indonesia. Empirically, green betel is used as an antibacterial to the wound. This study aims to determine the activity of ointment of ethanol extract of green betel leaves as antibacterial. Tests of antibacterial effects were performed in vitro and in vivo. Ethanol extract of green betel leaf is made by maceration. In vitro testing was done using micro dilution method to Staphylococcus aureus to determine the minimum inhibitory concentration of green leaf extract at concentrations of 3, 4, and 5%. Then the extract was made ointment and tested in vivo using rat consisting of the negative control group, the extract group (concentrations 3,4, and 5%), the positive control of gentamicin 0.1%. The test results were analyzed using Sapiro-Wilk and continued with ANAVA test and LSD test to find out the comparison of the results of the five treatment groups. The results of in vitro test showed that the minimum inhibit concentration of green betel leaf extract was 5%. The results of in vivo test showed the duration of wound healing increased with increasing concentration of green betel leaf extract. The result of normality test, Anava, and LSD test on in vivo test give the same result. Concentrations of 4 and 5% were effective concentrations with wound healing time activity were not significantly different with positive control (gentamicin 0.1%) with mean time of 6.20 ± 0.80 and 6.00 ± 0.71 days respectively. It can be concluded that the antibacterial test results in ointment are in line with in vitro test of leaf green betel extract which has potential as antibacterial.Key words: Green betel, Antibacterial, Ointment, Wound

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Antibacterial Activity of Ethanol Extract of Betel Leaf (Piper betle L.) Against Some Food Borne Pathogens

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v28i2.11817 ◽

2012 ◽

Vol 28 (2) ◽

pp. 58-63 ◽

Cited By ~ 15

Author(s):

M Mahfuzul Hoque ◽

Shemona Rattila ◽

M Asaduzzaman Shishir ◽

M L Bari ◽

Y Inatsu ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Foodborne Pathogens ◽

Ethanol Extract ◽

Moderate Temperature ◽

Piper Betle ◽

E Coli ◽

Betel Leaf ◽

Neutral Ph ◽

And Staphylococcus Aureus

The ethanol extract of Betel leaf (Piper betle L.), an indigenous climber plant of Bangladesh was screened for its antibacterial activity against some foodborne pathogens viz. Vibrio cholerae ATCC 6395, E. coli ATCC 25922, E. coli O157:H7 NCTC 12049, Shigella dysenteriae-1 MJ-84 and Staphylococcus aureus ATCC 25923. Experimental findings revealed that the ethanol extract of betel leaves potentially inhibit the growth of these foodborne pathogens. Minimum inhibitory concentration (MIC) was ranged from 0.625% (w/v) to 0.75% (w/v). The ethanol extract of betel leaf showed the highest MIC values for E. coli ATCC 25922 (0.625 mm), Vibrio cholera ATCC 6395 (0.625 mm), and Staphylococcus aureus ATCC 25923 (0.625 mm). It was revealed that the antibacterial activity was highest at around neutral pH and at moderate temperature. The highest temperature (100°C) and the pH (10.0) treated ethanol extract showed a little less activity than the maximum activity at moderate temperature and at neutral pH, suggesting that the ethanol extract of the betel leaf was to some extent high temperature and high pH tolerant. The ethanol extract of betel leaf might have a potential application as natural food preservative to improve the microbiological safety of the foods. DOI: http://dx.doi.org/10.3329/bjm.v28i2.11817 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 58-63

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Aktivitas antibakteri salep ekstrak etanol daun sirih hijau (Piper betleL.) Terhadap infeksi bakteri Staphylococcus aureus

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v5i2.117 ◽

2017 ◽

Vol 5 (2) ◽

pp. 56

Author(s):

Rissa Laila Vifta ◽

Muhammad Andri Wansyah ◽

Anita Kumala Hati

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Ethanol Extract ◽

Positive Control ◽

Piper Betle ◽

In Vitro Test ◽

In Vivo Test ◽

Betel Leaf

Abstrak Sirih hijau (Piper betle L.) adalah tanaman yang mudah dijumpai di Indonesia. Secara empiris, sirih hijau digunakan sebagai antibakteri pada luka. Penelitian ini bertujuan untuk mengetahui aktivitas salep ekstrak etanol daun sirih hijau sebagai antibakteri. Pengujian efek antibakteri dilakukan secara in vitro dan in vivo. Ekstrak etanol daun sirih hijau dibuat dengan cara maserasi. Pengujian secara in vitro dilakukan menggunakan metode mikrodilusi terhadap Staphylococcus aureus untuk mengetahui nilai konsentrasi hambat minimum ekstrak daun sirih hijau pada konsentrasi 3, 4, dan 5%. Kemudian ekstrak dibuat salep dan diuji secara in vivo menggunakan hewan uji tikus yang terdiri dari kelompok kontrol negatif, kelompok ekstrak (konsentrasi 3,4, dan 5%), serata pembanding kontrol positif gentamisin 0,1%. Hasil uji dianalisis menggunakan Sapiro-Wilk dan dilanjutkan dengan uji ANAVA serta uji LSD untuk mengetahui perbandingan hasil kelima kelompok perlakuan. Hasil uji in vitro menunjukkan bahwa konsentrasi hambat minimum ekstrak daun sirih yang optimal adalah 5%. Hasil uji in vivo menunjukkan lama kesembuhan luka meningkat seiring dengan bertambahnya konsentrasi ekstrak daun sirih hijau. Hasil uji normalitas, Anava, dan uji LSD pada uji in vivo memberikan hasil yang sejalan. Konsentrasi 4 dan 5% merupakan konsentrasi efektif dengan aktivitas waktu kesembuhan luka tidak berbeda bermakna dengan kontrol positif (gentamisin 0,1%) dengan rerata waktu berturut-turut 6,20±0,80 dan 6,00±0,71 hari. Dapat disimpulkan bahwa hasil uji antibakteri pada salep sejalan dengan uji secara in-vitro ekstrak daun Sirih Hijau yang memiliki potensi sebagai antibakteri.Kata kunci: Daun sirih hijau, Antibakteri, Salep, Luka Antibacterial activity from ointment contains extract of green betle leaves (Piper betle l.) to Staphylococcus aureus bacterial infection Abstract Green betel (Piper betle L.) is a plant that is easy to find in Indonesia. Empirically, green betel is used as an antibacterial to the wound. This study aims to determine the activity of ointment of ethanol extract of green betel leaves as antibacterial. Tests of antibacterial effects were performed in vitro and in vivo. Ethanol extract of green betel leaf is made by maceration. In vitro testing was done using micro dilution method to Staphylococcus aureus to determine the minimum inhibitory concentration of green leaf extract at concentrations of 3, 4, and 5%. Then the extract was made ointment and tested in vivo using rat consisting of the negative control group, the extract group (concentrations 3,4, and 5%), the positive control of gentamicin 0.1%. The test results were analyzed using Sapiro-Wilk and continued with ANAVA test and LSD test to find out the comparison of the results of the five treatment groups. The results of in vitro test showed that the minimum inhibit concentration of green betel leaf extract was 5%. The results of in vivo test showed the duration of wound healing increased with increasing concentration of green betel leaf extract. The result of normality test, Anava, and LSD test on in vivo test give the same result. Concentrations of 4 and 5% were effective concentrations with wound healing time activity were not significantly different with positive control (gentamicin 0.1%) with mean time of 6.20 ± 0.80 and 6.00 ± 0.71 days respectively. It can be concluded that the antibacterial test results in ointment are in line with in vitro test of leaf green betel extract which has potential as antibacterial.Key words: Green betel, Antibacterial, Ointment, Wound

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Isolasi, Identifikasi Senyawa Alkaloid Dan Uji Efektivitas Penghambatan Dari Ekstrak Daun Sirih (Piper betle L.) Terhadap Bakteri Staphylococcus epidermidis

Jurnal e-Biomedik ◽

10.35790/ebm.v8i2.28999 ◽

2020 ◽

Vol 8 (2) ◽

Author(s):

Gwendolyn L. Kapondo ◽

. Fatimawali ◽

Meilani Jayanti

Keyword(s):

Antibacterial Activity ◽

Optical Density ◽

Staphylococcus Epidermidis ◽

Leaf Extract ◽

Ethanol Extract ◽

Dilution Method ◽

Piper Betle ◽

Betel Leaf ◽

Before And After ◽

Density Values

Abstract: Betel leaf (Piper betle L) contains alkaloid compounds and has antibacterial and antiseptic functions. The purpose of this research was to isolated alkaloid compounds also to tested the effectiveness of inhibition of the betel leaf extract against the Staphylococcus epidermidis bacterial growth. Simplicia betel leaf was extracted used 96% of ethanol solvent. Antibacterial activity tested of betel leaf extract with a concentration variation of 25%, 20%, 15%, 10% and 5% using the liquid dilution method. The results of identification of alkaloid isolates used UV-Vis Spectrophotometry method can be known alkaloid compounds contained in betel leaves include alkaloids with indole base framework that was absorption at 262 nm and 274 nm wavelengths. The results of the effectiveness of betel leaf on Bacteria Staphylococcus epidermidis used the liquid dilution method showed that betel leaf extract with Optical density values at concentrations of 25%, 20%, 15% and 10% before and after incubation decreased respectively by -0.347, -0.304, -0.192 and -0.104, while at a concentration of 5% there was increase in Optical density values of 0.162. From the results of the research, it can be concluded that the ethanol extract of betel leaf contains an alkaloid with an indole base framework and was inhibitory activity against Staphylococcus epidermidis bacteria with a MIC at a concentration of 10%.Keywords: betel leaf, Staphylococcus epidermidis, alkaloid compound, liquid dilution. Abstrak: Daun Sirih (Piper betle L) mengandung senyawa alkaloid dan memiliki fungsi sebagai antibakteri dan antiseptik. Penelitian ini bertujuan untuk mengisolasi senyawa alkaloid dan menguji efektivitas penghambatan dari ekstrak daun sirih hijau terhadap pertumbuhan bakteri Staphylococcus epidermidis. Simplisia daun sirih hijau diekstraksi dengan menggunakan pelarut etanol 96%. Uji aktivitas antibakteri ekstrak daun sirih dengan variasi konsentrasi 25%, 20%, 15%, 10% dan 5% menggunakan metode dilusi cair. Hasil identifikasi terhadap isolat alkaloid menggunakan metode Spektrofotometri UV-Vis dapat diketahui senyawa alkaloid yang terkandung dalam daun sirih termasuk alkaloid dengan kerangka dasar Indol yang mempunyai serapan pada panjang gelombang 262 nm dan 274 nm. Hasil uji efektivitas daun sirih terhadap bakteri Staphylococcus epidermidis dengan metode dilusi cair menunjukan bahwa ekstrak daun sirih dengan nilai densitas optik pada konsentrasi 25%, 20%, 15% dan 10% sebelum dan sesudah inkubasi mengalami penurunan berturut-turut sebesar -0.347, -0.304, -0.192 dan -0.104, sedangkan pada konsentrasi 5% mengalami kenaikan nilai densitas optik sebesar 0.162. Dari hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak etanol daun sirih mengandung alkaloid dengan kerangka dasar indol dan memiliki aktivitas penghambatan terhadap bakteri Staphylococcus epidermidis dengan KHM pada konsentrasi 10%.Kata Kunci: daun sirih, Staphylococcus epidermidis, senyawa alkaloid, dilusi cair.

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Uji Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Sirih Hijau (Piper betle L.) dan Madu terhadap Bakteri Propionibacterium acnes

Proceeding of Mulawarman Pharmaceuticals Conferences ◽

10.25026/mpc.v10i1.355 ◽

2019 ◽

Vol 10 ◽

pp. 16-21

Author(s):

Lucia Bilasonya Sakramentia ◽

Nurul ◽

Fajar

Keyword(s):

Antibacterial Activity ◽

Leaf Extract ◽

Propionibacterium Acnes ◽

Sugar Content ◽

Inhibition Zone ◽

Flavonoid Glycoside ◽

Diffusion Method ◽

Piper Betle ◽

Betel Leaf ◽

High Sugar Content

Green betel leaf (Piper betle L.) and honey have been scientifically known to have antibacterial activity. The antibacterial activity of green betel leaf is caused by the presence of compounds, such as tannins, flavonoids, saponins, alkaloids, and terpenoids. While honey is due to the high sugar content, the acidity and compounds from the flavonoid, glycoside, and hydrogen peroxide groups. This study aims to determine the antibacterial activity of the combination of these extracts against Propionibacterium acnes. Antibacterial activity was tested using the well technique agar diffusion method with green betel leaf extract concentrations of 2.5%, 5%, 7.5%, 10%, and 12.5% and combined each concentration with 100% of honey. The results obtained by the extract antibacterial activity at concentrations of 2.5%, 5%, 7.5%, 10%, and 12.5%, each combined with 100% of honey respectively at 11,81 mm; 14,45 mm; 15,48 mm; 16,77 mm; and 14,68 mm against Propionibacterium acnes. The results of this study indicate the combination of green betel leaf extract with a concentration of 10% and 100% honey produces the most effective inhibition zone.

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Betel (Piper betle L.) leaf ethanolic extracts dechlorophyllized using different methods: antioxidant and antibacterial activities, and application for shelf-life extension of Nile tilapia (Oreochromis niloticus) fillets

RSC Advances ◽

10.1039/d1ra02464g ◽

2021 ◽

Vol 11 (29) ◽

pp. 17630-17641

Author(s):

Mohamed Tagrida ◽

Soottawat Benjakul

Keyword(s):

Shelf Life ◽

Nile Tilapia ◽

Ethanolic Extract ◽

Antibacterial Activities ◽

Life Extension ◽

Piper Betle ◽

Refrigerated Storage ◽

Betel Leaf ◽

Nile Tilapia Oreochromis Niloticus ◽

Ethanolic Extracts

Betel leaf ethanolic extract dechlorophyllized using sedimentation (BLEE-SED) exhibited enhanced antioxidant and antibacterial activities. BLEE-SED could extend the shelf-life of Nile tilapia fillets during refrigerated storage (4 °C) up to 9 days.

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