Optimization of culture media to produce Bacillus subtilis strain QST 713 in a handcrafted bioreactor

Objective. To optimize a nutrient medium based on fertilizers for the cultivation of Bacillus subtilis in an Airlift-type handcrafted bioreactor. Design/Methodology/Approach. Twenty-seven nutrient media, fixed by combining five factors with three levels, including sucrose, ammonium sulfate, triple superphosphate, UltraK® formula, and B. subtilis inoculum (Serenade® Max) were tested in a 50L handcrafted by the authors. The variables monitored in the media were absorbance, dissolved oxygen, pH, and temperature. The first was the one that was considered for optimization as it is the indirect indicator of bacterial growth. On the statistical analysis, the option "Larger is better" was chosen for Signal/Noise for the ANOVA of the main effects according to the Taguchi method. Results. The highest level of sucrose, together with the lowest level of triple superphosphate were determinants for maximum growth of Bacillus in the time studied. On the other hand, the components such as ammonium sulfate, UltraK® formula, or the amount of inoculum were not significant, which means that they can be added from the mid to low levels. Study limitations/Implications. This new information can be scaled to bioreactors of 2500 L for B. subtilis that we have previously developed. Finding/Conclusions. Maximum bacterial growth depends on a good supply of sucrose, limiting triple superphosphate. Additionally, it is prudent to decrease additions of ammonium sulfate because it reduces dissolved oxygen in the nutrient medium.

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Study of the Effect of the Earthworm (Aporrectodea molleri) Cutaneous Excreta on Bacterial Growth

International Journal of Ecology ◽

10.1155/2021/5518568 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Lamia Yakkou ◽

Sofia Houida ◽

Mohammed Raouane ◽

Souad Amghar ◽

Abdellatif El Harti

Keyword(s):

Bacterial Growth ◽

Growth Rates ◽

Culture Media ◽

Chemical Constituents ◽

High Growth ◽

Maximum Growth ◽

Plant Growth Promoting Bacteria ◽

E Coli ◽

Conventional Medium

The effect on the bacterial growth of Aporrectodea molleri cutaneous excreta has given very satisfactory results. The cutaneous excreta were obtained using the electrical stimulation technique, recently developed by our laboratory. This technique made it possible to collect the excreta in sufficient quantities while ruling out any possible interference with the chemical constituents of the soil. The effectiveness of cutaneous excreta, tested as culture media at different concentrations on bacterial growth, was justified not only by the high growth rates but also by the concentrations used, which were several times lower than those of the conventional medium (nutrient agar). Indeed, the maximum growth rate recorded by E. coli and P. fluorescens is, respectively, 1.32 times and 2.99 times greater than that observed on the conventional medium. These higher levels are obtained at optimal excretion concentrations which are, respectively, 8 times and 133.33 times lower than the concentration of the conventional medium. The efficacy interval that delimited the excretion concentrations tested covers 8 successive concentrations ranging from 0.0075% to 1% for P. fluorescens, while for E. coli, this interval covers only three concentrations ranging from 0.06% at 0.25%. The growth rates of P. fluorescens are always higher than those of E. coli. The higher efficiency of excretions on the growth of P. fluorescens shows that this strain is more suited to use these excretions as a source of nutrients and to react positively to the stimulation of growth. These results, obtained in vitro, prove that, in the natural environment, the production of lubricating and viscous cutaneous excretions does not only have the role of facilitating the movement and digging of galleries by earthworms but also of enriching the soil in nutrients and growth factors with high added values for the activation and development of plant growth-promoting bacteria essential for soil fertility.

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The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.12 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Hussein A Kadhum ◽

Thualfakar H Hasan2

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Bacterial Growth ◽

Broad Spectrum ◽

Inhibitory Activity ◽

Bacterial Pathogens ◽

Inhibitory Ability ◽

Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

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Evaluation of the growth and sporulation of different entomopathogenic fungi in different liquid and solid media at varied concentrations

International Journal of Bioassays ◽

10.21746/ijbio.2017.08.003 ◽

2017 ◽

Vol 6 (8) ◽

pp. 5459

Author(s):

Chandra Teja K. ◽

Rahman S. J.

Keyword(s):

Entomopathogenic Fungi ◽

Large Scale ◽

Insect Pests ◽

Culture Media ◽

Virulent Strain ◽

Carbon Sources ◽

Nitrogen Sources ◽

Maximum Growth ◽

Nutritional Requirements ◽

Virulent Strains

Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides world-wide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.

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Endoglucanase Produced by Bacillus subtilis Strain CBS31: Biochemical Characterization, Thermodynamic Study, Enzymatic Hydrolysis, and Bio-industrial Applications

Biotechnology and Bioprocess Engineering ◽

10.1007/s12257-019-0338-5 ◽

2020 ◽

Vol 25 (1) ◽

pp. 104-116 ◽

Cited By ~ 1

Author(s):

Sudip Regmi ◽

Yoon Seok Choi ◽

Young Kyun Kim ◽

Md Maruf Khan ◽

Sang Hun Lee ◽

...

Keyword(s):

Bacillus Subtilis ◽

Enzymatic Hydrolysis ◽

Biochemical Characterization ◽

Industrial Applications ◽

Thermodynamic Study ◽

Subtilis Strain

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ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters

Microbiology ◽

10.1099/mic.0.024661-0 ◽

2009 ◽

Vol 155 (4) ◽

pp. 1080-1092 ◽

Cited By ~ 28

Author(s):

A. A. Bartosik ◽

J. Mierzejewska ◽

C. M. Thomas ◽

G. Jagura-Burdzy

Keyword(s):

Pseudomonas Aeruginosa ◽

Fluorescence Microscopy ◽

Bacterial Growth ◽

Complete Loss ◽

Physiological Parameters ◽

Wild Type ◽

Partial Removal ◽

Partner Protein ◽

The One ◽

Mutant Phenotypes

Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy of fixed cells showed that ParB mutants lacking the C-terminal domain or HTH motif formed multiple, less intense foci scattered irregularly, in contrast to the one to four ParB foci per cell symmetrically distributed in wild-type P. aeruginosa. All parB mutations affected both bacterial growth and swarming and swimming motilities, and increased the production of anucleate cells. Similar effects were observed after inactivation of parA of P. aeruginosa. As complete loss of ParA destabilized its partner ParB it was unclear deficiency of which protein is responsible for the mutant phenotypes. Analysis of four parB mutants showed that complete loss of ParB destabilized ParA whereas three mutants that retained the N-terminal 90 aa of ParB did not. As all four parB mutants demonstrate the same defects it can be concluded that either ParB, or ParA and ParB in combination, plays an important role in nucleoid distribution, growth and motility in P. aeruginosa.

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Cloning, sequencing and expression of the xylanase gene from a Bacillus subtilis strain B10 in Escherichia coli

Bioresource Technology ◽

10.1016/j.biortech.2005.04.011 ◽

2006 ◽

Vol 97 (6) ◽

pp. 802-808 ◽

Cited By ~ 38

Author(s):

Junli Huang ◽

Guixue Wang ◽

Li Xiao

Keyword(s):

Escherichia Coli ◽

Bacillus Subtilis ◽

Subtilis Strain ◽

Xylanase Gene ◽

Sequencing And Expression

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Complete Genome Sequence of Bacillus subtilis subsp. subtilis Strain ∆6

Genome Announcements ◽

10.1128/genomea.00759-16 ◽

2016 ◽

Vol 4 (4) ◽

Cited By ~ 3

Author(s):

Daniel R. Reuß ◽

Andrea Thürmer ◽

Rolf Daniel ◽

Wim J. Quax ◽

Jörg Stülke

Keyword(s):

Bacillus Subtilis ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Full Genome Sequence ◽

Subtilis Strain ◽

Full Genome ◽

Biotechnological Applications ◽

A Genome ◽

Subtilis Subsp

Bacillus subtilis ∆6 is a genome-reduced strain that was cured from six prophages and AT-rich islands. This strain is of great interest for biotechnological applications. Here, we announce the full-genome sequence of this strain. Interestingly, the conjugative element ICE Bs 1 has most likely undergone self-excision in B. subtilis ∆6.

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Intracellular serine proteinase of Bacillus subtilis strain Marburg 168. Comparison with the homologous enzyme from Bacillus subtilis strain A-50

Biochemical Journal ◽

10.1042/bj1790333 ◽

1979 ◽

Vol 179 (2) ◽

pp. 333-339 ◽

Cited By ~ 13

Author(s):

A Y Strongin ◽

D I Gorodetsky ◽

I A Kuznetsova ◽

V V Yanonis ◽

Z T Abramov ◽

...

Keyword(s):

Bacillus Subtilis ◽

Serine Proteinase ◽

Subtilis Strain ◽

Bacterial Strains ◽

Terminal Sequence ◽

Gramicidin S ◽

Intracellular Enzymes ◽

Sepharose 4B ◽

Homologous Enzyme ◽

Strict Selection

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.

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