scholarly journals Isolation and Molecular Marker Detection of Badh2 Gene from Aromatic Rice Germplasm Resources in Southern Henan

2019 ◽  
pp. 35-45
Author(s):  
Bo Peng ◽  
Yu Zhu ◽  
Zi-Yu Wang ◽  
Juan Peng ◽  
Lu-Lu He ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Chromosome 8 ◽  
Aromatic Rice ◽  
Germplasm Resources ◽  
Rice Varieties ◽  
Exon 2 ◽  
Rice Germplasm ◽  
Functional Molecular Markers ◽  
Badh2 Gene

The production of aroma in aromatic rice is due to the increase of 2-acetyl-1-pyrroline (2-AP) precursor substances caused by the functional deletion of Badh2 gene on chromosome 8, and the accumulation of 2AP makes rice produce aroma. In this study, Badh2 gene was isolated and cloned from 18 representative aromatic rice cultivars in Southern Henan, and the bioinformatics analysis of Badh2 gene was carried out. Meanwhile, seven functional molecular markers developed by Badh2 gene were used to detect and analyze Badh2 gene in 18 aromatic rice varieties from Southern Henan. The results showed that the coding region of Badh2 gene was 1509 bp in length. It contained 15 exons and 14 introns, and encoded 503 amino acids. There are many types of variation of the Badh2 gene in the 18 aromatic rice varieties. According to the variation of Badh2 gene, the tested aromatic rice varieties could be divided into three groups, among which Xinxianggeng 1, Xiangnuo 25, Heixiangdao 193 and Xiangbao 2 were concentrated in group Ⅰ, while the other 14 kinds of aromatic rice were concentrated in group II. Seven functional molecular markers of Badh2 gene were used to detect different varieties mutation types in exon 2, exon 4~5, exon 7 and exon 13 of Badh2 gene. No aromatic rice varieties with different mutation types were found in promoter region, exon 12 and exon 14 of Badh2 gene. Therefore, our results provide important information for understanding the genetic basis of fragrant genes in aromatic rice germplasm resources in Southern Henan and breeding new varieties of high-quality aromatic rice using molecular marker-assisted selection.

scholarly journals The Determination of Aromatic Character of Several Local Rice Varieties using Phenotypic Analysis and Molecular DNA

2021 ◽  
Vol 22 (2) ◽  
pp. 111
Author(s):  
Wahyu Indra Duwi Fanata ◽  
Syafira Fatihatul Husna
Keyword(s):  
Dna Markers ◽  
Rice Variety ◽  
Genetic Mutation ◽  
Pcr Analysis ◽  
Betaine Aldehyde Dehydrogenase ◽  
Aromatic Rice ◽  
Phenotypic Analysis ◽  
Rice Varieties ◽  
Aromatic Character ◽  
Badh2 Gene

The pandan scent in aromatic rice has been known as the result of 8 bp deletions and 3 Single Nucleotide Polymorphisms (SNPS) in BADH2 gene, which produce non-functional betaine aldehyde dehydrogenase (BADH) enzyme. Several DNA markers for aromatic character based on mutation in BADH2 gene have been developed. In our experiment, we analysed the presence of aromatic character in four local rice variety such as Merah Wangi, Pendok, Genjah Arum, and Mentik Wangi Susu using KOH method and DNA molecular method using three DNA markers to detect mutation that responsible for the development of aromatic character. Phenotype analysis using KOH method showed that Merah Wangi, Genjah Arum, and Mentik Wangi Susu produce pandan scents. PCR analysis using Bradbury and Badex7-5, and RM223 markers showed the presence of BADH2 mutation in Merah Wangi and Mentik Wangi Susu, whereas Pendok and Genjah Arum did not show BADH2 mutation using those used three markers. Our results indicate that among four investigated local rice, only Merah Wangi and Mentik Wangi Susu are categorized as aromatic rice whereas Pendok and Genjah Arum are non-aromatic.Keywords: genetic analysis, aromatic rice, specific markers, genetic mutation.

scholarly journals Applications of molecular markers for bacterial blight resistant varieties in rice (Oryza sativa L.)

2017 ◽  
Vol 9 (4) ◽  
pp. 2309-2314
Author(s):  
A. Premkumar ◽  
Manoj Kumar
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Bacterial Blight ◽  
Oryza Sativa L ◽  
Single Gene ◽  
Durable Resistance ◽  
Genetic Resistance ◽  
Recurrent Parent ◽  
Rice Varieties ◽  
Novel Technologies

Bacterial blight is one of devasting disease in almost all rice growing countries. The most effective, eco-nomic and environmental strategy for control of this disease is to develop rice varieties with genetic resistance. However, new pathotype has overcome single gene for resistance in the new cultivars. So, plant breeders are con-centrating to develop high yielding varieties with durable resistance using novel technologies. Molecular marker technology has progressed tremendously in the past decade for genetic improvement of field crops. Molecular markers can improve efficiency of breeding in different ways for trait in segregating population like identify plants with target gene in maximum recovery portion of recurrent parent. The transfer of two or three genes into single variety with the help of molecular marker is expected to lead to more durable resistance. Thus, thus review describes progress made in the development of bacterial blight resistance rice varieties using Marker Assisted Selection.

scholarly journals Sensory Test and Molecular Marker Based-Selection for Aromatic Rice in the F3 Progenies

Agrikultura ◽  
2020 ◽  
Vol 31 (2) ◽  
pp. 109
Author(s):  
Nono Carsono ◽  
Amalia Purdianty ◽  
Santika Sari ◽  
Citra Bakti
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Agronomic Traits ◽  
Brown Planthopper ◽  
Aromatic Rice ◽  
Sensory Test ◽  
Resistant Lines ◽  
Antisense Primer ◽  
Selection For ◽  
Fgr Gene

Aromatic rice is a special type of rice that highly preferred by people in Asia due to the presence of aroma. Aroma in rice is determined by 2-acetyl-1-pyrroline (2AP) compound which is controlled by a recessive fgr gene. A hybridization between cv. Sintanur (aromatic rice) and PTB33 (non-aromatic, resistant to brown planthopper/BPH) has been done in order to develop aromatic rice lines that resistant to BPH. In the F2 progeny, molecular marker-based selection and bioassay for the brown planthopper resistant lines have been carried out; however selection for the aromatic trait has not been performed yet. The objective of this study was to obtain the F3 progeny’s individual with aromatic trait. Sensory test was conducted by KOH 1.7% solution, meanwhile molecular markers applied were ESP (External Antisense Primer), IFAP (Internal Fragrant Antisense Primer), INSP (Internal Non fragrant Sense Primer) and EAP (External Antisense Primer). Eighty-eight plants from two selected (SP#31 and SP#224) F3 lines progenies derived from cv. Sintanur and PTB33 have been evaluated in this study. Detection by molecular markers found seventy-five genotypes (85.23%) were homozygous recessive (aromatic rice) and one was heterozygous (non-aromatic). Eighty-five (96.59%) genotypes were aromatic as detected by sensory test alone. Seventy-two (81.82%) genotypes were categorized as aromatic rice based on sensory test and molecular markers. Due to inconsistency results from each method alone, it is advised both methods to be applied to ensure the reliability and the accuracy since aroma in rice is affected by genetic composition and environment conditions. Selected genotypes will be continued for breeding program in developing aromatic rice with improved agronomic traits.

scholarly journals Genetic Diversity Evaluation and Conservation of Kam Fragrant Glutinous Rice (Oryza sativa L.) Germplasm in Southeast Guizhou, China

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1898
Author(s):  
Qi-Yi Lei ◽  
Jiang-Ju Zhou ◽  
Yong Xiong ◽  
Wen-Hua Zhang ◽  
Jing Luo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Cultural Anthropology ◽  
Germplasm Resources ◽  
Growth Environment ◽  
Folk Classification ◽  
Rice Germplasm ◽  
Glutinous Rice ◽  
Traditional Cultures ◽  
High Level

The genetic diversity of rice germplasm is the basis for increases in rice yield and quality. The collection, assessment, and protection of the genetic diversity of rice germplasm is important for achieving sustainable agriculture and assuring food security. Many underdeveloped indigenous areas have abundant and valuable rice germplasm resources. However, in-depth assessments of the genetic diversity of rice germplasm from these areas and studies related to protecting these traditional cultures are not available. In this study, from 2005 to 2016, the authors have conducted in-depth evaluation of the genetic diversity of Kam fragrant glutinous rice germplasm resources in southeast Guizhou by using multidisciplinary comprehensive methods such as ethnobotany, cultural anthropology, and modern molecular markers. In total, 376 Kam fragrant glutinous rice samples from 42 villages in the Dong community in southeast Guizhou were collected. Agronomic traits of panicles were complex and exhibited diversity. Some varieties had good disease resistance and adaptation to cold and wet climates. The Dong people named the Kam fragrant glutinous rice varieties by using seven elements, including diverse traits, growth environment, and origin. Traditional folk classification, in addition to morphology and biological analysis using molecular markers, indicates that Kam fragrant glutinous rice includes 91 varieties. Kam fragrant glutinous rice comprises a very high number of varieties, most of which are japonica-type and exhibit a high level of genetic diversity. The traditional folk classification of Kam fragrant glutinous rice by the Dong community is consistent with the biological classification. The traditional naming of Kam fragrant glutinous rice provides an important reference for understanding its genetic diversity. The high level of genetic diversity in Kam fragrant glutinous rice is not only related to the natural environment of the area but also tightly linked with the abundant and diverse Dong ethnic traditional cultures, which has led to protection of Kam fragrant glutinous rice’s genetic diversity.

scholarly journals IDENTIFIKASI MARKA GEN KETAHANAN HAWAR DAUN BAKTERI PADA GALUR PADI INTRODUKSI DAN GALUR DIHAPLOID

2014 ◽  
Vol 14 (2) ◽  
pp. 101-109
Author(s):  
Ovi Prasetya Winandari ◽  
Aris Tjahjoleksono . ◽  
Dwinita Wikan Utami
Keyword(s):  
Molecular Markers ◽  
Selection Process ◽  
Control Plant ◽  
Bacterial Pathogen ◽  
Leaf Blight ◽  
Breeding Program ◽  
Bacterial Leaf Blight ◽  
Rice Varieties ◽  
Rice Germplasm ◽  
Resistant Varieties

Identification marker of bacterial leaf blight (BLB) gene resistance on introducted and dihaploid rice germplasm. Bacterial leaf blight (BLB), caused by bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating diseases in rice. The use of BLB-resistant rice varieties is one of the most efficient ways to protect rice from this disease. BLB-resistant varieties can be produced through the breeding program by using the diverse  rice germplasm. The objective of this research was to identify BLB resistance gene on 37 introducted and dihaploid rice lines derived from wide genetic background double crossing from local rice : IR54/Parekaligolara and Bio110/Markuti, by using the molekular markers. As control plant  used 23 differential varieties (monogenic lines/IRBB) and TN1 (susceptible).  All plants tested were inoculated by 3 selected dominant BLB races (Race III, IV dan VIII). The selected of 19 polymorphism molecular markers used to identify the BLB resistance genes on rice lines tested. The result of this research showed that 4 rice lines were resistance to all BLB races tested. Three molecular markers were specific associated with resistant gene to Race III (Xa7-STS40, Xa1-STS14 and Xa4-STS50); Race IV (Xa1-STS5, Xa4-STS50 and Xa26-STS1), and Race VIII (Xa21-STS6, Xa7-RM20590 and Xa7-STS40). These markers could be utilized for the selection process the developmen of BLB resistance rice lines breeding program. 

scholarly journals Agro-Morphological Characterization and Assessment of Variability in Aromatic Rice Germplasm

2012 ◽  
Vol 37 (1) ◽  
pp. 1-8 ◽  
Author(s):  
M Parikh ◽  
NK Motiramani ◽  
NK Rastogi ◽  
B Sharma
Keyword(s):  
Genetic Parameters ◽  
Agronomic Traits ◽  
Morphological Characterization ◽  
Aromatic Rice ◽  
Spikelet Sterility ◽  
Plant Parts ◽  
Rice Germplasm ◽  
Spikelets Per Panicle ◽  
Plant Habit ◽  
Selection Of

The present studies were carried out to characterize seventy-one aromatic rice germplasm from IGKV, Raipur. These germplasm were characterized and grouped on the basis of anthocyanin pigmentation, plant habit, and awning character. On the basis of pigmentation distribution in 10 plant parts, a total of twelve groups were formed with group one having no pigmentation and group twelve with pigmentation in 9 plant parts. On the basis of plant habit and awning character, three groups of each were formed. The genetic parameters for the ten agronomic traits indicated that the selection of genotypes may be done for fertile spikelets per panicle, spikelet density, spikelet sterility percentage, and hundred seed weight. Thus, on the basis of above characters, the genotypes Tulsi Mala, Baanspati, Ganga Balu, Samund Chini, Tulsi Amrit, Dudh Dhan, Kari Gilas, Shankar Jeera, and Jata Shankar may be selected for future study. DOI: http://dx.doi.org/10.3329/bjar.v37i1.11168 Bangladesh J. Agril. Res. 37(1): 1-8, March 2012

scholarly journals Growth, Yield and Proximate Composition of Aromatic Rice as Influenced by Inorganic and Organic Fertilizer Management

2018 ◽  
Vol 10 (2) ◽  
pp. 211-219
Author(s):  
Md. Jafrul Islam SUMON ◽  
Tuhin Suvra ROY ◽  
Md. Nazmul HAQUE ◽  
Salma AHMED ◽  
Kanika MONDAL
Keyword(s):  
Protein Content ◽  
Proximate Composition ◽  
Green Manure ◽  
Organic Fertilizer ◽  
Growth Yield ◽  
Fat Content ◽  
Carbohydrate Content ◽  
Rice Grain ◽  
Aromatic Rice ◽  
Rice Varieties

Integrated use of organic and inorganic fertilizers can improve crop productivity and sustain soil health and fertility. To gain insights into the response of green manure and chemical fertilizer, the present study was conducted to evaluate the growth, yield and proximate composition of aromatic rice varieties in Aman season at the research farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from July to December 2014. The experiment was set up in split-plot design with three aromatic rice varieties in main plots and six fertilizer levels in subplots. ‘Raniselute’ variety produced the highest plant height, dry matter weight hill-1, straw yield (7.81 t ha-1), biological yield (9.05 t ha-1), ash (1.59%), and fat content (2.81%). ‘BRRI dhan34’ gave the maximum number of effective tillers hill-1 (12.74), panicle length (27.93 cm), number of filled grains panicle-1 (192.5), 1,000-grain weight (17.22 g), grain yield (2.26 t ha-1), harvest index (29.99%), and carbohydrate content (77.63%). Application of 80% recommended doses of NPKSZn + green manure 3.5 t ha-1 showed better performance for getting the maximum growth, yield components and yield compared to other treatments. Recommended doses of NPKSZn showed the highest carbohydrate content (77.63%) and lowest moisture (8.75%) and ash content (1.29%). The maximum fat content (3.07%) and minimum carbohydrate content (76.53%) was obtained from 60% recommended doses of NPKSZn + green manure 7 t ha-1. Application of 20 and 40% recommended doses of NPKSZn + green manure 14 and 10.5 t ha-1 produced the highest moisture content (10.43%) and lowest protein content (8.26%) in rice grain. Green manure 17.5 t ha-1 produced the highest ash (1.79%), protein content (9.06%) and lowest fat content (2.51%).

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