scholarly journals Histopathological Study on The Effect of Neuregulin-1 Against Cardiotoxicity Induced By Trastuzumab in Adult Mice

2018 ◽  
pp. 63-77
Keyword(s):  
Intraperitoneal Injection ◽  
Cardiac Tissue ◽  
Statistical Significance ◽  
Cardiac Complications ◽  
Control Group ◽  
Histopathological Study ◽  
Neuregulin 1 ◽  
Adult Mice ◽  
Histological Score ◽  
Group B

Objective: This work carried out to investigate the potential therapeutic effect of Neuregulin-1 against cardiotoxicity induced by trastuzumab in male mice. Materials and Methods: Forty five adult male and female mice (weighing 35-50 gm) were divided into 3 groups randomly, each group contains 15 animals, group A which received distilled water as control by using intraperitoneal injection (IP) route for 10 days group B, received Trastuzumab as 6 mg/kg/day by using intraperitoneal injection route for 10 days while group C, received Neuregulin-1 (2.5 mg/day/animal) just before the administration of Trastuzumab (6mg/kg/day) given by IP route for 10 days. The significance of difference were tested by using ANOVA followed by Tukey test. Statistical significance depends on the P<0.05. Results: The histological score of cardiac tissue changes showed that a highly increase in mice administrated with trastuzumab when compared to control group while pretreatment with Neuregulin-1 , made a decrease in histological changing score when compared with trastuzumab group. The percentage of immunohistochemical score of stain uptake for TNF-α and IL-1β expression in cardiac tissue shows a highly increase in mice administrated when compared with control group, furthermore, pretreated with neuregulin-1 showed a decrease in the percentage of staining when compared with trastuzumab group. Conclusion: Neuregulin-1 can be used as a cardioprotective in cardiac complications of cancer treatment by its ability to attenuate the cardiotoxic effect of trastuzumab by decreasing in histological changing score and decrease in the percentage of staining when compared with trastuzumab group.

scholarly journals The Influence of the Physiotherapeutic Program on Selected Static and Dynamic Foot Indicators and the Balance of Elderly Women Depending on the Ground Stability

2021 ◽  
Vol 18 (9) ◽  
pp. 4660
Author(s):  
Joanna Matla ◽  
Katarzyna Filar-Mierzwa ◽  
Anna Ścisłowska-Czarnecka ◽  
Agnieszka Jankowicz-Szymańska ◽  
Aneta Bac
Keyword(s):  
Elderly Women ◽  
Statistical Significance ◽  
Exercise Program ◽  
Control Group ◽  
Balance Assessment ◽  
Motor Functions ◽  
Static Test ◽  
Group A ◽  
Group B ◽  
The Impact

Seniors are a constantly growing group of people in many societies. It is necessary to develop physiotherapeutic programs to improve their mobility. The aim of this study was to assess the impact of the physiotherapeutic program conducted unstable ground on selected indicators of motor functions of elderly women. Sixty women (60–80 years) participated in the research. Group A (N = 20) underwent a 12-week physiotherapeutic program on stable ground, group B (N = 20) followed an exercise program on unstable ground, and group C (N = 20) (control group) had no therapeutic intervention. The effects of the therapy were assessed by using a FreeMed platform (foot load analysis) and a Biosway balance system. The results were compared using ANOVA (the one-way analysis), the Kruskal–Wallis test and also the post hoc tests (Tukey’s test and the multiple comparison test). In group A, a statistically significant change was observed in the static test and balance assessment, in group B this was observed in the static and dynamic foot tests and balance assessment, in group C, no statistical significance was achieved. The authors’ physiotherapeutic program had a statistically significant effect on changes in the balance and selected indicators of the motor functions of the examined people. Comparing the results before and after the therapy more improvement changes were noted in women training on an unstable ground compared to women training on a stable ground.

Sports Hernia in National Hockey League Players

2012 ◽  
Vol 41 (1) ◽  
pp. 107-110 ◽  
Author(s):  
Andre Jakoi ◽  
Craig O’Neill ◽  
Christopher Damsgaard ◽  
Keith Fehring ◽  
James Tom
Keyword(s):  
Statistical Significance ◽  
Return To Play ◽  
National Hockey League ◽  
Control Group ◽  
Sports Hernia ◽  
Level Of Evidence ◽  
Athletic Pubalgia ◽  
Hockey Players ◽  
Group A ◽  
Group B

Background: Athletic pubalgia is a complex injury that results in loss of play in competitive athletes, especially hockey players. The number of reported sports hernias has been increasing, and the importance of their management is vital. There are no studies reporting whether athletes can return to play at preinjury levels. Purpose: The focus of this study was to evaluate the productivity of professional hockey players before an established athletic pubalgia diagnosis contrasted with the productivity after sports hernia repair. Study Design: Cohort study; Level of evidence, 3. Methods: Professional National Hockey League (NHL) players who were reported to have a sports hernia and who underwent surgery from 2001 to 2008 were identified. Statistics were gathered on the players’ previous 2 full seasons and compared with the statistics 2 full seasons after surgery. Data concerning games played, goals, average time on ice, time of productivity, and assists were gathered. Players were divided into 3 groups: group A incorporated all players, group B were players with 6 or fewer seasons of play, and group C consisted of players with 7 or more seasons of play. A control group was chosen to compare player deterioration or improvement over a career; each player selected for the study had a corresponding control player with the same tenure in his career and position during the same years. Results: Forty-three hockey players were identified to have had sports hernia repairs from 2001 to 2008; ultimately, 80% would return to play 2 or more full seasons. Group A had statistically significant decreases in games played, goals scored, and assists. Versus the control group, the decreases in games played and assists were supported. Statistical analysis showed significant decreases in games played, goals scored, assists, and average time on ice the following 2 seasons in group C, which was also seen in comparison with the control group. Group B (16 players) showed only statistical significance in games played versus the control group. Conclusion: Players who undergo sports hernia surgeries return to play and often perform similar to their presurgery level. Players with over 7 full seasons return but with significant decreases in their overall performance levels. Less veteran players were able to return to play without any statistical decrease in performance and are likely the best candidates for repair once incurring injury.

scholarly journals Biochemical and Histopathological study Of The Proteinaceous Compounds Separated From Aqueous Extract Of Marus Albul L. Fruit in alloxan diabetic mice

2019 ◽  
Vol 24 (3) ◽  
pp. 44
Author(s):  
Shihab A. Al-Bajari
Keyword(s):  
Aqueous Extract ◽  
Intraperitoneal Injection ◽  
Glycogen Content ◽  
Control Group ◽  
Histopathological Study ◽  
Molecular Weight Determination ◽  
Diabetic Mice ◽  
Total Lipids ◽  
Vacuolar Degeneration ◽  
Liver Tissues

This work was concerned with isolating and molecular weight determination of the proteinous compounds isolated from the cold and boiled aqueous extract of Marus Albal L. using different biochemical techniques . Also this study indicated the effect of the proteinaceous compounds Ac, Bc and Ab, Bb on some biochemical parameters including glucose, cholesterol and total lipids levels in blood serum and glycogen content in liver tissues in normal and diabetic mice . A single intraperitoneal injection of these isolated compounds with a dose of 75 mg / kg body weight was used .As well as pancreas were removed and stained with H and E of pancreatic sections. Results indicated that intraperitoneal injection of insulin and all the proteinaceous compounds Ac , Bc and Ab, Bb obtained by gel filtration chromatography from the plant used had hypoglycemic effect on serum glucose level in normal and diabetic mice. The rate of decrease was from (18.2) to (43.7) % in the normal and form (29.57) to (50.20) % in the diabetic mice respectively. While compounds (Bb) showed a negligible to an increasing effect (3.91) % in the normal and (7.99)% in the diabetic mice. Also the highest decrease was obtained for compound (Ac), this decrease were (16.8 )% and (15.73) % for serum cholesterol levels in the normal and the diabetic mice respectively .As well as the highest decrease was obtained for compound (Ac) , this decrease were(23.62) % and (28.88) % for serum total lipids levels in the normal and the diabetic mice respectively. Also a decrease between (5.8 , 14.1) % and (6.4 , 12.9) %  glycogen content in liver tissues was obtained when the normal and in the diabetic mice respectively were injected intraperitoneally by the proteinaceous compounds Ac, Bc and Ab , Bb from plants used. Finally alloxan induced diabetic mice group, the endocrine pancreas histologically showed decreased in the size and number of Langerhans islets with vacuolar degeneration and necrosis of almost all cell in the atrophied islets as compared with control group. The histomorphometic study of the pancreas of the treated group didn't show a significant change of the pancreatic tissue.   http://dx.doi.org/10.25130/tjps.24.2019.046

The effect of clenbuterol on body composition in spontaneously eating tumour-bearing mice

1993 ◽  
Vol 13 (6) ◽  
pp. 325-331 ◽  
Author(s):  
Anders Hyltander ◽  
Gösta Svaninger ◽  
Kent Lundholm
Keyword(s):  
Body Composition ◽  
Food Intake ◽  
Tumour Growth ◽  
Statistical Significance ◽  
Dry Weight ◽  
Daily Basis ◽  
Control Group ◽  
Tumour Bearing ◽  
Adult Mice ◽  
Adrenoceptor Agonist

The aim of this study was to investigate the effect of a selective β2-adrenoceptor agonist, clenbuterol, on body composition in tumour-bearing adult and growing mice. Therefore, adult female C57/BL6 mice (n = 20) were inoculated subcutaneously with a 3-methylcholanthrene-induced sarcoma and divided into two identical groups. One group received injections twice a day of clenbuterol corresponding to 1 mg/kg body weight, the other group received sham injections. Growing mice (n = 20) were similarly divided after tumour inoculation into one study group with clenbuterol injections and one control group. The growing animals were sacrificed on day 11 after commencement of treatment, the adult mice on day 16. Clenbuterol treatment had no statistically significant effect on accumulated food intake or body composition in the adult mice. However, fooe intake in these animals increased numerically compared to control animals after day 12 of the study. Tumour growth was also unaffected. The growing animals displayed an increased carcass dry weight with borderline significance (p = 0.06) and an increased quadriceps muscle fat free dry weight after clenbuterol treatment. Tumour growth was not affected. Food intake measured on a daily basis was significantly increased in the growing clenbuterol treated animals and accumulated food intake was increased with a trend towards statistical significance (p = 0.06). The results support the suggestion that treatment with a selective β2-adrenoceptor agonist does not improve body composition in tumour-bearing adult mice relying on spontaneous food intake while growing animals may benefit from such treatment.

scholarly journals Role of methyl prednisolone in reducing mortality and morbidity in hair dye poisoning

2017 ◽  
Vol 4 (4) ◽  
pp. 1078
Author(s):  
Akhtar A. ◽  
Verma B. D.
Keyword(s):  
Low Cost ◽  
Statistical Significance ◽  
Cardiac Complications ◽  
Hair Dye ◽  
Mortality And Morbidity ◽  
Medical College ◽  
Methyl Prednisolone ◽  
Medical Health Care ◽  
Group B

Background: Hair dye containing paraphenylenediamine (PPD) is widely used in India because of its free availability and low cost. PPD produces local as well as systemic toxic effects when applied topically and/or ingested. This study was undertaken to find the role of methyl prednisolone in reducing mortality and morbidity in hair poisoning and to study the cardiac complications of hair dye poisoning.Methods: The present study was carried out among 1000 cases admitted in the Department of Medicine, MLB Medical College, Jhansi with history/characteristic and clinical features of hair dye poisoning. The complication and mortality profile was studied. Statistical significance was drawn with the help of Microsoft excel 2003.Results: Mortality in acute hair dye poisoning cases was 23.20%. Majority of deaths (50.04%) occurred within 24 hours of hair dye ingestion and were because of asphyxia and respiratory failure. Mortality in 44.96% cases was after 24 hours and were mainly due to renal failure and its related complications hyperkalaemia and cardiac complication. Lower mortality was observed in group A (8.40%) as compared to group B (8.0%) and C (4.0%) in association with ST and T wave changes.Conclusions: PPD is available quite freely and used extensively. Awareness regarding its poisonous effects should be increased among the medical health care workers.

scholarly journals Oxidative stress profile following the co-administration of cisplatin and resveratrol in female rats: a preliminary study

2021 ◽  
Vol 25 (2) ◽  
pp. 134-145
Author(s):  
Izuchukwu Azuka Okafor ◽  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Normal Control ◽  
Intraperitoneal Injection ◽  
Female Rats ◽  
Control Group ◽  
Stress Profile ◽  
Body Tissues ◽  
Group A ◽  
Group B

Introduction: Cisplatin is one of the most widely used drugs for the treatment of various cancers but has oxidative tissue damage as one of its side effects. This study investigated the oxidative stress profile in some important body tissues following the co-administration of cisplatin (CIS) and resveratrol (RSV). Methods: Thirty-five adult female rats with an average body weight of 162g were divided into 5 groups (n=7) and used for this experimental study. Group A served as the normal control group and received distilled water only. Group B received only a single dose intraperitoneal injection of 10mg/kg CIS. Groups C, D and E were orally given 5, 10 and 20mg/kg of RSV respectively for 7 days, starting 24h after a single CIS dose intraperitoneal injection of 10mg/kg. Selected body tissues were harvested for oxidative stress profiling at the end of the experiment. Results: CIS significantly increased malondialdehyde levels and decreased glutathione, superoxide dismutase and catalase levels in all the tissues assessed (ovary, uterus, liver, kidney, pancreas, stomach and spleen) when compared to the normal control. The RSV treatment caused the reversal of these effects; malondialdehyde levels were significantly decreased, while glutathione, superoxide dismutase and catalase levels were significantly increased across all the examined tissues. Conclusion: RSV at different doses could be effective in the management of CIS-induced oxidative stress and lipid peroxidation across some body tissues. However, this effect may be dependent on the dose of CIS and RSV.

scholarly journals Efficacy of Crest Herbal Toothpaste in “Clearing Internal Heat”: A Randomized, Double-Blind Clinical Study

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jia-Xu Chen ◽  
Yue-Yun Liu ◽  
Shao-Xian Wang ◽  
Xiao-Hong Li
Keyword(s):  
Rating Scale ◽  
Statistical Significance ◽  
Internal Heat ◽  
Control Group ◽  
Clinical Test ◽  
Double Blind ◽  
Intention To Treat ◽  
Group A ◽  
Group B ◽  
Control Group Group

Objective. Evaluation of the efficacy of Crest Herbal Crystal Toothpaste in “clearing internal heat.”Methods. This was a randomized, double-blind, controlled parallel design clinical test of a product that was already on the market. 72 subjects were randomly assigned to control group (group A with Colgate Herbal Salty Toothpaste) or treatment group (group B with Crest Herbal Crystal Toothpaste) with ratio of 1 : 2. Subjects were instructed to brush with 1g toothpaste for 2 minutes each time, 2 times per day in a 4-aweek test period; measurement with the rating scale on the efficacy of “clearing internal heat” for the herbal toothpaste was done at baseline, 2 weeks, and 4 weeks of toothpaste usage.Results. The rating scale on efficacy of “clearing internal heat” for the herbal toothpaste reveals that the primitive points of 72-case intention-to-treat (ITT) analysis and 67-case per-protocol (PP) analysis for subjects in group A and subjects in group B were found to be reduced progressively with statistical significance (P<0.05). The overall effective rates for group A and group B were, respectively, 62.50%, 56.25% (ITT) and 62.50%, 60.64% (PP). The statistical results indicated that the symptoms of fire-heat for both groups of subjects have been improved after application of toothpaste.Conclusion. The efficacy of Crest Herbal Crystal Toothpaste in “clearing internal heat” was confirmed by the trial as compared to Colgate Herbal Salty Toothpaste. And its efficacy was objectively evaluated by the rating scale on efficacy of “clearing internal heat.”

scholarly journals Noise stress on female mice impair oocyte developmental potential.

2019 ◽  
Vol 26 (06) ◽  
Author(s):  
Maria Yousaf ◽  
Hina Kundi ◽  
Abdullah Qamar
Keyword(s):  
Histological Study ◽  
Treated Group ◽  
Control Group ◽  
Developmental Potential ◽  
Noise Stress ◽  
Medical College ◽  
Adult Mice ◽  
Group A ◽  
Group B ◽  
Animal House

Objectives: To find out the effects of noise stress on variations in number of ovarian follicles in mice. Study Design: Randomized control trial, laboratory based. Place and Duration of Study: Anatomy department, Army Medical College Rawalpindi, it was conducted from April to May 2014. Material and Method: 20 adult mice (BALBc strain) female weighing 25-27 grams were taken for this study on day first of their estrous cycle. Two groups comprising of 10 rats each were made. Control group was given name tag of group A and was kept in animal house under normal heathy environment for one month. Noise stress of 100 decibels for 06 hours per day for one month was given to experimental group B. Mice were dissected after 1 month. Mice right ovaries were examined for size, shape, color and weight. The specimen were processed and embedded. Hematoxyline and Eosin stained slides were prepared for histological study. Follicular count (primary, secondary and tertiary follicles) was done and noted. For statistical analysis SPSS 20 was used. The tests applied was independent sample’s t test for descriptive statistics. Results: There was a significant decrease in primary, secondary/antral and graafian follicular count in the ovary of Group B (Experimental). Conclusion: The noise stress prominently affected the follicular count in ovaries of noise stress treated group.

scholarly journals Weekly evaluation of estrogen α and progesterone receptors in the mammary epithelium of women after the use of combined oral hormonal contraceptives for one month

Mastology ◽  
2020 ◽  
Vol 30 (Suppl 1) ◽  
Author(s):  
Joaquim Teodoro de Araújo ◽  
Celso Kazuto Taniguchi ◽  
Anastasio Berrentini Junior ◽  
Rogério Fenile ◽  
Afonso Celso Pinto Nazário
Keyword(s):  
Statistical Significance ◽  
Progesterone Receptors ◽  
Mammary Epithelium ◽  
Hormonal Contraceptives ◽  
Control Group ◽  
Environmental Carcinogens ◽  
Paracrine Factors ◽  
Increased Risk ◽  
Group A ◽  
Group B

The increased risk of breast cancer (BC) is the most controversial adverse effect of combined oral hormonal contraceptives (COHC). An evaluation of hormone receptors (HR) revealed that their expressions are inversely proportional to the rate of cell proliferation, with a hierarchy where proliferative cells would be controlled by paracrine factors released by positive HR cells. Thus, the greater the proliferation, the greater the risk of the actions of environmental carcinogens. Compare the expressions by immunohistochemistry (IHC) of estrogen α (ERα) and progesterone receptors (PR) weekly in the mammary epithelium (ME) of patients using COHC for one month with those in the natural cycle. Retrospective cohort study of 118 women, 42 of whom were excluded and with a final sample of 76 women. Study group (A) consisted of 31 users of COHC with 30 µg of ethinyl estradiol (EE) and 150 µg of levonorgestrel (L), and control group (B), 45 non-users of COHC. In parity comparison, the Fisher's exact test was used, and for the mean ages, the Student’s t-test for independent samples. The Generalized Estimation Equation (EEG) model was used to evaluate ERα and PR counts over the four weeks and compare them. The Analysis of Variance (ANOVA) with two fixed factors and the Kolmogorov-Smirnov test were used to compare the total ERα and PR counts. Statistical analyses were performed using the SPSS 20.0 and STATA 12 programs, with a significance level of 5% (p≤0.05). As for age, group B had a mean age of 23.7±5.9 years compared to group A, with a mean age of 20.5±5.1 years, statistically significant p=0.016. With regard to parity, group A presented 83.9% of nulligravida versus 73.3% in group B, with p=0.164 without statistical significance. According to GEE, group A had higher mean percentages of ERα (p<0.001) and PR (p<0.001) when compared to women in the control group. The expressions of the IHC of the ERα and PR in percentages, weekly in the ME of the patients in the users of COHC presented higher mean percentages of ERα and PR, both statistically significant, when compared with non-users.

scholarly journals Comparative Evaluation of Plaque Inhibitory and Antimicrobial Efficacy of Probiotic and Chlorhexidine Oral Rinses in Orthodontic Patients: A Randomized Clinical Trial

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Shreya Shruti Shah ◽  
Supriya Nambiar ◽  
Deepa Kamath ◽  
Ethel Suman ◽  
Bhaskaran Unnikrishnan ◽  
...  
Keyword(s):  
Oral Health ◽  
Antimicrobial Agents ◽  
Statistical Significance ◽  
Control Agent ◽  
Allocation Concealment ◽  
Control Group ◽  
Natural Agents ◽  
Orthodontic Patients ◽  
Plaque Control ◽  
Group B

Background. Antimicrobial agents serve as an effective adjunct in plaque control, and chlorhexidine has been the gold standard. However, the philosophy that natural agents are better for children's oral health is on the rise. Probiotic technology represents a breakthrough approach to maintain oral health by utilizing natural beneficial bacteria commonly found in healthy mouths. Aim and Objective. To compare efficacy of probiotic and chlorhexidine oral rinses in orthodontic patients. Materials and Methods. 30 healthy patients undergoing fixed orthodontic treatment were randomly selected for the study by block randomization and allocation concealment and were divided into three groups: group a, 0.2% chlorhexidine mouthwash; group b, probiotic mouthwash; and group c, a control group. Results. Probiotic and chlorhexidine groups had significantly decreased plaque indices as compared to the control group. However, greater improvement was seen in the gingival indices than plaque indices with better results in the probiotic group than the chlorhexidine group. No statistical significance was observed in the streptococcus count of probiotic and chlorhexidine groups at the end of the intervention period. Conclusion. The comparison of probiotics to chlorhexidine has proven that probiotics are as effective as chlorhexidine as an adjunctive chemical plaque control agent.

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