scholarly journals UJI TOKSISITAS EKSTRAK ETANOL TERPURIFIKASI BIJI MAHONI (Switenia mahagoni)

2019 ◽  
Vol 6 (2) ◽  
pp. 360-362
Author(s):  
Virsa Handayani ◽  
Ahmad Najib ◽  
Rezki Amriati Syarif ◽  
Abdullah Mahmud ◽  
La Hamidu ◽  
...  
Keyword(s):  
Toxicity Test ◽  
Condensed Tannin ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Test Solution ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Medical Plant ◽  
Varied Concentration

Mahoni is a medical plant which have the potential as drug. The aims of this research were to analysis phytochemical content and to test the toxicity of ethanol extract of seed from Mahoni. The Phytochemicals that analyzed were total phenolic, total flavonoid and condensed tannin. Toxicity test was assessed using BSLT method. Extraction was done by maseration method using ethanol as the solvent. In BSLT method, the shrimp larvae were placed in a series of test solution of varied concentration. The value of LC50 were obtained based on calculation of shrimp larvae lethality percentage using probit analysis. LC50 values of ethanol extract were 0,95 ppm.

scholarly journals Short Communication: Phytochemical screening and toxicity of ethanol extract of Sauropus androgynus

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
AWALUDIN AWALUDIN ◽  
KARTINA KARTINA ◽  
DIANA MAULIANAWATI ◽  
WASMEN MANALU ◽  
ANDRIYANTO ANDRIYANTO ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Toxicity Test ◽  
Short Communication ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Mortality Data ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phytochemical Content ◽  
Tiger Shrimp

Abstract. Awaludin, Kartina, Maulianawati D, Manalu W, Andriyanto, Septiana R, Arfandi A, Lalang Y. 2020. Short Communication: Phytochemical screening and toxicity of ethanol extract of Sauropus androgynus. Biodiversitas 21: 2966-2970. Katuk (Sauropus androgynus L.) (SAL) is a plant used to increase the production of breast milk for nursing mothers and lactating mammalian animals because this plant contains high sterols. Phytosterols are plant sterols known to play a role in the synthesis and secretion of progesterone and estradiol.  Progesterone and estradiol are hormones that are involved in controlling the reproduction and mammary gland growth and development in mammalian animals. Estradiol also stimulates vitellogenin synthesis in oviparous animals such as avian and fish.  The study was designed to determine the phytochemical contents and the level of toxicity of the SAL ethanol extract. The research was conducted in 4 phases i.e., (i) extraction of SAL samples using ethanol 70%, (ii) the analysis of phytochemical content of SAL extract using GCMS, (iii) toxicity test of SAL extract in giant tiger shrimp larvae, and (iv) data analysis. The results of GCMS analysis showed that the SAL extract was dominated by the fatty acid group (62.92%), phenol (15.3%), and terpenoids (4.03%). These compounds have roles in animal reproduction and production. The mortality data of giant tiger shrimp larvae were analyzed using probit analysis to determine the LC50 of ethanol extract of SAL. The results of toxicity test for 24 hours showed that the LC50 of the ethanol extract of SAL was 552.208 mg/L. The phytochemical analysis showed that SAL extract has dominant concentrations of fatty acid, phenol, and terpenoid that could be used to improve the reproduction and production of fish.

scholarly journals Toxicity Test of Roots, Stems and Leaves of Lemongrass (Cymbopogon nardus)

2019 ◽  
Vol 6 (2) ◽  
pp. 78-85
Author(s):  
Waras Nurcholis ◽  
Mustika Weni ◽  
Rizki Fitria ◽  
Najmah ◽  
Kornelia Rosvita Manek ◽  
...  
Keyword(s):  
Toxicity Test ◽  
Ethanol Extract ◽  
Solution Concentration ◽  
Probit Analysis ◽  
Test Solution ◽  
Root Extract ◽  
Lc50 Value ◽  
Cymbopogon Nardus ◽  
Percent Mortality ◽  
Stem Extract

Lemongrass traditionally can be used as a mixture of water bath for rheumatic patients, antiseptic medicine, headache reducer, unbend the leech bite and overcome insect bite, but, the bioactivity potential of citronella lemongrass still remains unknown. The content of citronella lemongrass is known to have secondary metabolites such as flavanoid, phenolic, and terpenoid. The aim of this study is to determine the LC50 value (Lethality Concentration 50) in the root, stem, and leaves part of citronella lemongrass. Citronella lemongrass is extracted with maceration method using 96% ethanol solvent. Rendement value of leaf extract is greater than the value of stem and root extract, which is equal to 8.69% for stem extract, 3.73% for root extract and 9.70% for leaves extract. A toxicity test was performed by using the BSLT method, shrimp larvae were inserted into the test solution with each different solution concentration. LC50  values were obtained based on the calculation of percent mortality of shrimp larvae by using probit analysis. LC50 ethanol extract 96% of citronella lemongrass leaf is 67.841 μg/ml, citronella lemongrass stem is 31.604 μg/ml and citronella lemongrass root is 10.489 μg/ml. LC50 value shows that ethanol extract 96% of root, stem, and leaf of citronella lemongrass have no bioactivity potential due to the value of LC50 is greater than 1000 μg/ml. Keywords: BSLT, Cymbopogon nardus, cytotoxicity, LC50

scholarly journals Toxicity Test of Roots, Stems and Leaves of Lemongrass ( Cymbopogon nardus )

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Waras Nurcholis ◽  
Mustika Weni ◽  
Rizki Fitria ◽  
Najmah ◽  
Kornelia Rosvita Manek ◽  
...  
Keyword(s):  
Toxicity Test ◽  
Ethanol Extract ◽  
Solution Concentration ◽  
Probit Analysis ◽  
Test Solution ◽  
Root Extract ◽  
Lc50 Value ◽  
Cymbopogon Nardus ◽  
Percent Mortality ◽  
Stem Extract

Lemongrass traditionally can be used as a mixture of water bath for rheumatic patients, antiseptic medicine, headache reducer, unbend the leech bite and overcome insect bite, but, the bioactivity potential of citronella lemongrass still remains unknown. The content of citronella lemongrass is known to have secondary metabolites such as flavanoid, phenolic, and terpenoid. The aim of this study is to determine the LC50 value (Lethality Concentration 50) in the root, stem, and leaves part of citronella lemongrass. Citronella lemongrass is extracted with maceration method using 96% ethanol solvent. Rendement value of leaf extract is greater than the value of stem and root extract, which is equal to 8.69% for stem extract, 3.73% for root extract and 9.70% for leaves extract. A toxicity test was performed by using the BSLT method, shrimp larvae were inserted into the test solution with each different solution concentration. LC50  values were obtained based on the calculation of percent mortality of shrimp larvae by using probit analysis. LC50 ethanol extract 96% of citronella lemongrass leaf is 67.841 μg/ml, citronella lemongrass stem is 31.604 μg/ml and citronella lemongrass root is 10.489 μg/ml. LC50 value shows that ethanol extract 96% of root, stem, and leaf of citronella lemongrass have no bioactivity potential due to the value of LC50 is greater than 1000 μg/ml. Keywords: BSLT, Cymbopogon nardus, cytotoxicity, LC50

scholarly journals Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

2021 ◽  
Vol 332 ◽  
pp. 08005
Author(s):  
Aminah Dalimunthe ◽  
Dewi Pertiwi ◽  
Mahatir Muhammad ◽  
Denny Satria
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Litsea Cubeba ◽  
Ferric Reducing Antioxidant Power ◽  
Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

scholarly journals Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Irmanida Batubara ◽  
Komariah Komariah ◽  
Apong Sandrawati ◽  
Waras Nurcholis
Keyword(s):  
Principal Component ◽  
Ethanol Extract ◽  
Chemometric Analysis ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Pharmacological Activities ◽  
Frap Assay ◽  
Phytochemical Content ◽  
Ethanol Extracts ◽  
Mcf 7

AbstractOrthosiphon aristatus (Blume) Miq. of the Lamiaceae family, called as kumis kucing in Indonesia, is a valuable medicinal plant for their pharmacological properties. The present study comprised of fifteen genotypes of O. aristatus was undertaken to evaluate the genotypes based on phytochemical content and pharmacological activities of leaves ethanol extract. Chemometric analysis (correlation and principal component analysis) was also used to investigate the genetic variability based on phytochemical content and pharmacological activities of O. aristatus genotypes. Results of phytochemical characterization showed that total phenolic ranged from 1.48 to 36.08 (maximum in A15) mg GAE/g DW, total flavonoid ranged from 0.10 to 3.07 (maximum in A15) mg QE/g DW, sinensetin ranged from 0.36 to 4.02 (maximum in A11) mg/g DW, and rosmarinic acid ranged 0.06 to 7.25 (maximum in A7) mg/g DW. Antioxidant activity was tested using DPPH and FRAP assay. Antioxidant results showed that DPPH ranged from 1.68 to 15.55 (maximum in A15) μmol TE/g DW and FRAP ranged from 0.07 to 1.60 (maximum in A1 and A7) μmol TE/g DW. The genotype A8 showed the highest cytotoxic activities against HeLa (66.25%) and MCF-7 (61.79%) cell lines. Maximum α-glucosidase inhibitory activity was recorded in genotype A2 with the value of 62.84%. The genotypes A1, A2, A7, A11, and A15 were identified as superior based on their phytochemicals content and pharmacological activities coupled with chemometric analysis. This finding is important for breeding studies and also the pharmaceutical perspective of O. aristatus.

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Correlation between total flavonoid content and total phenolic content on antioxidant activity of ethanol extracts from three cultivars of papaya leaves

2020 ◽  
Vol 11 (2) ◽  
pp. 1883-1887
Author(s):  
Prakit Chaithada ◽  
Praweena Whenngean ◽  
Radchada Fungfueng ◽  
Saowanee Maungchanburee
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ethanol Extracts ◽  
Dpph Radical Scavenging

The aim of this study was to elucidate the correlation between total flavonoid content and total phenolic content on antioxidants activity of ethanol extracts from three cultivars of papaya leaves: 'Holland,' 'Khak Dam' and 'Red Lady.' All crude extracts were investigated to find antioxidant capacity in DPPH radical scavenging. The result indicated that the ethanol extract of ‘Red Lady’ papaya leaves exhibited the highest level of DPPH radical scavenging activity with the IC50 of 0.18 mg/mL, followed by the ‘Khak Dam’ and ‘Holland’ papaya leaves having an IC50 value of 0.24 and 0.44 mg/mL, respectively. The ethanol extract from 'Red Lady' papaya leaves showed the highest level of total flavonoid content (TFC) of 276.72 ± 1.04 µgQE/g DW and total phenolic content (TPC) of 169.85 ± 6.54 mgGAE/g DW. All three cultivars showed a distinctive correlation between IC50 and the content of both total flavonoid and total phenolic with a negative relationship of Pearson’s correlation of -0.922 and -0.940, respectively.

scholarly journals Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
YEKKI YASMIN ◽  
Lenni Fitri ◽  
FAUZIAH ◽  
FITRI WASLIYAH
Keyword(s):  
Antibacterial Activity ◽  
Toxicity Test ◽  
Apis Cerana ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacterial Extract ◽  
Lc50 Value ◽  
Antibacterial Assay

Abstract. Yasmin Y, Fitri L, Fauziah, Wasliyah F. 2019. Isolation of bacteria from Apis cerana hive, their antibacterial potency and cytotoxicity. Biodiversitas 20: 2733-2738. This study aimed to identify bacteria from Apis cerana hive; to determine their antibacterial activity, and cytotoxic effect of hive extract as well as bacterial extract. Identification of bacteria isolated from the hive was carried out based on morphological and biochemical characters. The antibacterial assay of beehive bacteria isolates was done by disk diffusion method against Staphylococcus aureus and Escherichia coli. Toxicity test of beehive extract and beehive bacterial extract were carried out using Brine Shrimp Lethality Test (BSLT) with six final concentrations (32 ppm, 64 ppm, 126 ppm, 250 ppm, 500 ppm, and 1000 ppm). The LC50 value was determined by probit analysis using SPSS. Bacterial isolation showed there were four bacterial isolates from A. cerana hive were the genus Bacillus (BSL1, and BSL3), the genus Micrococcus (BSL2) and the genus Neisseria (BSL4). The antibacterial assay showed that BSL4 isolate has the highest antibacterial activity against S. aureus with the diameter of the inhibitory zone was 32.6 mm. Toxicity test showed that A. cerana hive extract and bacterial ethanol extract from A. cerana hive had LC50 value of 67,744 ppm and 86.985 ppm respectively and categorized as toxic.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

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