scholarly journals Listeria Monocytogenes Contamination Study in Food from Plant Origin in the Market

2019 ◽  
Vol 4 (3) ◽  

Fresh food from plants is fresh food that is consumed directly or after cutting, I have minimal processing. Therefore PSAT to be consumed must be safe from aspects of microbial contamination and physical chemical contamination. There are several types of pathogenic microbes, which can contaminate PSAT and can cause disease disorders. One microbe, which includes pathogenic microbes and potentially contaminates PSAT, is Listeria monocytogenes. Microbes Listeria monocytogenes are pathogenic in certain groups of people in small amounts. To know the prevalence of Listeria monocytogenes, studies were carried out, by taking samples at locations, and selected commodities. The locations chosen are West Java, Banten and DKI Jakarta in two traditional markets in each province. While the selected commodities are bean sprouts, tomatoes and cabbage. Sampling was carried out by random method, with the population in accordance with the number of vegetable traders in that location. Testing of samples for Listeria monocytogenes contamination is carried out in accredited laboratories. From the results of testing of 300 (three hundred) samples, consisting of 100 samples of bean sprouts, 100 samples of tomatoes, and 100 samples of cabbage, all test samples were declared negative or unexposed to Listeria monocytogenes.

2019 ◽  
Vol 4 (3) ◽  

Fresh food from plants (PSAT) is fresh food consumed directly or after experiencing minimal processing. Therefore PSAT to be consumed must be safe from aspects of microbial contamination and physical chemical contamination. There are several types of pathogenic microbes, which can contaminate PSAT and can cause disease disorders. One microbe, which includes pathogenic microbes and potentially contaminates PSAT, is Salmonella sp. Salmonella sp microbes are pathogenic to society in general. Some health problems caused by PSAT contaminated with Salmonella sp, disorders of the digestive tract, in the form of diarrhea, or typhus. Land and water used in the cultivation process and handling of it contaminated with salmonella sp. To know the prevalence of Salmonella sp in PSAT, a study was carried out, by taking samples at locations, and selected commodities. The locations chosen are West Java, Banten and DKI Jakarta in two traditional markets in each province. While the selected commodities are bean sprouts, tomatoes and cabbage. Sampling was carried out by random method, with the population in accordance with the number of vegetable traders in that location. Testing of samples for Listeria monocytogenes contamination is carried out in accredited laboratories. From the results of testing of 300 (three hundred) samples, consisting of 100 samples of bean sprouts, 100 samples of tomatoes, and 100 samples of cabbage, 4 samples of bean sprouts (or 4%) were stated to be positively contaminated with Salmonella sp. 3 samples of cabbage (3%) were positive for salmonella contamination. In other words, Salmonella sp contamination is found in 4 sample bean sprouts from 100 samples, pollution also occurs in 2 tomato samples from 100 samples and occurs in 3 samples of cabbage from 100 samples.


2016 ◽  
Vol 62 (4) ◽  
pp. 27-31
Author(s):  
Bui Manh Ha ◽  
Duong Thi Giang Huong ◽  
Luong Thi Hong Xuyen

Abstract Traditional markets play a major role in socio-economics and constitutes a significant aspect of Vietnamese culture. However, wastewater streams discharged from the markets are generally characterized by a lot of inorganic nutrients and organic substances originated from fresh food processing units. They could lead to serious water contamination if discharged without proper treatment. This study applied microalgae Chlorella sp. for eliminating inorganic nutrients (NO3−-N, NH4+-N and PO43−-P) and organic carbon (Chemical oxygen demand-COD) from wastewater of the Binh Dien market. The removal efficiencies reached for NH4+-N > 86%, for NO3−-N > 72%, and for PO43−-P > 69%, respectively, at algal density of 49 × 104 cell mL−1, and for COD > 96% at algal density of 35 × 104 cell mL−1 after five cultivating days. The effluence satisfied the Vietnamese standard, column B, of the National technical regulation on industrial wastewater (QCVN 40:2011/BTNMT). The results demonstrated that the culture system composed of green algal Chlorella sp. could be a potential candidate for the removal of nutrients and organic carbon by a wastewater treatment process from the Binh Dien market.


2002 ◽  
Vol 65 (7) ◽  
pp. 1088-1092 ◽  
Author(s):  
SUN-YOUNG LEE ◽  
KYUNG-MI YUN ◽  
J. FELLMAN ◽  
DONG-HYUN KANG

This study was undertaken to compare the efficacies of chlorous acid (268 ppm), sodium hypochlorite (200 ppm), and lactic acid (2%) in eliminating total mesophilic microorganisms, Salmonella Typhimurium, and Listeria monocytogenes on commercial mung bean sprouts immediately after treatment and during posttreatment refrigerated storage. Treatment with sodium hypochlorite for 10 min did not reduce the total aerobic count. However, treatment with lactic acid and chlorous acid for 10 min initially reduced the total aerobic count by 0.6 and 0.8 log CFU/g, respectively, and maintained the same level or a lower level of the total aerobic count during the storage time. Treatment with chlorous acid reduced Salmonella Typhimurium from 5.0 log to undetectable levels (<0.48 log CFU/g), and the pathogen remained undetectable over a 9-day storage period. Treatment with lactic acid resulted in an initial 3-log reduction and further reduced the number of Salmonella Typhimurium cells to undetectable levels after 3 days. For L. monocytogenes, treatment with chlorous acid resulted in an initial 5-log reduction, and treatment with lactic acid resulted in a 2-log reduction at the beginning and undetectable levels after 9 days. When chemically injured cells were investigated by the selective overlay method, no statistical difference was observed (P < 0.05) between the number of injured cells recovered following treatment with chlorous acid and the number of bacteria counted on selective media, whereas sodium hypochlorite generated more injured cells than the other treatments did. These data suggest that treatment with chlorous acid may be useful in reducing total mesophilic microorganisms, Salmonella Typhimurium, and L. monocytogenes in commercial mung bean sprouts.


2019 ◽  
Vol 37 (2) ◽  
pp. 185
Author(s):  
Wari Pawestri ◽  
Gagak Dhony Satria ◽  
Nisa Hakimah ◽  
Doddi Yudhabuntara

Food products of animal origin which are free from biological and chemical contamination are an absolute requirement of food safety. Antibiotics residues in the food of animal origin is one of the chemical contaminants in food are harmful/hazardous to human health. Tetracycline is an antibiotic that is often used in the fishing industry. The study aims to detect the occurrence of tetracycline residues in tilapia sold in traditional markets in Kota Yogyakarta. The research was conducted with detection test (detect disease). The study used 61 samples of tilapia fish from 16 traditional markets in Kota Yogyakarta. Tilapia meat samples are prepared according to the Association of Official Analytical Chemistry (AOAC). The existence of tetracycline residues in meat can be detected by high performance liquid chromatography (HPLC). Residue analysis in fish meat was conducted at the Department of Pharmacology, Faculty of Veterinary Medicine of Gadjah Mada University using HPLC Shimadzu version 6.1. The results showed that 24 samples of tilapia meat marketed in Kota Yogyakarta was positively contained tetracycline antibiotic residues. A total of 19 samples contained residues above maximum residue limits (MRL). The conclusion of the study is 31% of tilapia marketed in Kota Yogyakarta contains tetracycline residues above MRL. Surveillance of residues in food of animal origin and further research in terms of reducing antibiotic residue in tilapia meat is highly recommended to improve the quality and safety of food of animal origin.


Author(s):  
Manreet Singh Bhullar ◽  
Ana Monge-Brenes ◽  
Bridget Perry ◽  
Lillian Nabwiire ◽  
Angela Laury-Shaw

Grocery stores handle fresh produce in large quantities daily. According to the Food and Drug Administration Food Code, food is to be stored at least 15 cm above the floor, and all foods shall be protected from any source of contamination or otherwise discarded. It is reported in the literature that dropped produce could be a potential source of microbial contamination. Both consumers and employees often drop produce on the floor and place it back into a display case or bin, which could potentially serve as a source of contamination. This study aims to determine the bacterial transfer rate on different produce types when dropped onto Listeria monocytogenes contaminated floor surfaces for various contact times. Apples, peaches, and romaine lettuce were dropped separately onto carpet and tile surfaces from a distance of 1 m and held for 5s, 1min, 10min, 1h, and 4h.  Results showed that transfer from all produce types occurred from both the carpet (10.56%) and tile (3.65%) surfaces. Still, percent transfer was not statistically significant among different times used in this study (p>0.05). Dropped romaine lettuce had the most transfer (28.97%) from both the surfaces combined, followed by apple(s) (8.80%) and peach(es) (7.32%) with minimal transfer. Even with a low transfer level, grocery stores should include signage to alert consumers not to pick up dropped produce, and train their employees accordingly.


2002 ◽  
Vol 12 (2-3) ◽  
pp. 273-283 ◽  
Author(s):  
Colin Hill ◽  
Paul D Cotter ◽  
Roy D Sleator ◽  
Cormac G.M Gahan

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Gadafi Iddrisu Balali ◽  
Denis Dekugmen Yar ◽  
Vera Gobe Afua Dela ◽  
Priscilla Adjei-Kusi

Microbes are found all over the globe with some few exceptions, including sterilized surfaces. They include normal flora that is nonpathogenic, which contribute to the larger percentage, and pathogenic species which are few. Hence, the activities of humans cannot be completely separated from microbes. Thus, many pathogenic microbes have found their way into fresh fruits and vegetables which are a great source of a healthy diet for humans. The growing demand for fresh fruits and vegetables has necessitated larger production. The larger production of vegetables within the shortest possible time to meet the growing demand has placed them at a higher risk of contamination with the pathogenic microbes, making the safety of consumers uncertain. Study of sources of contamination and type of pathogenic etiological agents isolated from fresh fruits and vegetables includes Bacillus cereus, Campylobacter jejuni, Clostridium botulinum, E. coli O157: H7, Listeria monocytogenes, Salmonella spp., Shigella, Staphylococcus, and Vibrio cholera. Several measures have proven to be effective in controlling contamination of microbes and they include the establishment of surveillance systems to monitor the production chain and thoroughly washing vegetables with vinegar water. Saltwater and other washing techniques are effective but caution should be taken to make sure one does not use one cycle of water for washing all vegetables. The consumption of fresh fruits and vegetables is still encouraged by this review but significant measures must be taken to check the safety of these products before consumption.


Author(s):  
Tam√°s S√°ray ◽  
√âva Andr√°ssy ◽  
L√°szl√≥ M√©sz√°ros ◽  
J√≥zsef Farkas ◽  
Csilla Moh√°csi-Farkas

2014 ◽  
Vol 77 (8) ◽  
pp. 1407-1410 ◽  
Author(s):  
YONI DARMAWAN SUGIRI ◽  
GRETA GÖLZ ◽  
TONGKORN MEEYAM ◽  
MAXIMILIAN P. O. BAUMANN ◽  
JOSEF KLEER ◽  
...  

This study was conducted to determine the prevalence and quantify the number of Listeria monocytogenes in fresh chicken carcasses sold in traditional markets and supermarkets in Bandung, West Java, Indonesia, and to determine the antimicrobial resistance patterns of the isolated L. monocytogenes strains. The overall prevalence of L. monocytogenes in chicken carcasses was 15.8% (29/184). When comparing samples from traditional markets and supermarkets, no significant difference in the L. monocytogenes prevalence was detectable (15.2 versus 16.3%). Of the samples, 97.3% had L. monocytogenes counts <100 CFU/g, 2.2%had L. monocytogenes counts between 101 and 1,000 CFU/g, and 0.5%had L. monocytogenes counts of 1,001 to 10,000 CFU/g. Of the isolates, 27.6% were resistant to at least one of the 10 antimicrobials tested, with the major resistant phenotypes to penicillin (17.2%), ampicillin (6.9%), and erythromycin (6.9%). All 29 isolates recovered in this study were grouped into the molecular serogroup IIb, comprising the serovars 1/2b, 3b, and 7.


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