scholarly journals Optimization of Roasting Conditions on the Bioactive Compounds and their Antioxidant Power from Opuntia fiscus-Indica Seeds using Response Surface Methodology (RSM)

2020 ◽  
Vol 11 (3) ◽  
pp. 10510-10532
Keyword(s):  
Bioactive Compounds ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Total Phenolic ◽  
Opuntia Ficus Indica ◽  
Antioxidant Power ◽  
Box Behnken Design ◽  
Total Sugars ◽  
Total Flavonoids Content ◽  
Experimental Response

This work is aimed to optimize the effects of different roasting factors on the bioactive compound’s antioxidant capabilities of opuntia ficus indica seeds by using DPPH and ABTS assays. The Box-Behnken design (BBD) was applied to optimize the roasting temperature (X1:60-200 °C), roasting time (X2:10-50 min), and extraction time (X3: 3–6h). The optimal conditions of roasting applied in order to optimize the greatest content of the responses were: TPC (Total Phenolic Content): X1:200 °C, X2: 50 min, and X3: 5.49 h with 103.5±0.76 GAE/g extract experimental response, TFC (Total Flavonoids content): X1:200°C, X2:50min, and X3:3.16 h with 104.29±0.54 mg QE/g extract experimental response, TTC (Total Condensed Tannins Content ): X1: 200°C, X2: 50min, X3: 6h with 10,16±0.89 mg QAE/g extract experimental response, TSC ( Total Sugars Content ): X1: 60°C, X2:50 min, X3:5.12 h with 40.35±0.99 mg D-Glu E/g extract an experimental response. The maximal antioxidant power by DPPH and ABTS assays was recorded at a strong temperature of roasting. Moreover, the significant correlations were obtained by the principal component analysis (PCA), the first step, between the bioactive compounds, and on the second step, between their antioxidant capacities (DPPH, ABTS). Hierarchical Cluster Analysis (HCA) classified extracts into three clusters on the basis of measured antioxidant activity characteristics. Therefore, the result of this work indicates that roasted Opuntia ficus Indica seeds could be used as a significant human nutrient.

scholarly journals Optimization The Effect of Roasting Conditions by Central Composite Design (CCD) Method on The Antioxidant Compounds of Opuntia Ficus Indica Seeds From Morocco

2020 ◽  
Author(s):  
Chakir El Guezzane ◽  
Hamza El Moudden ◽  
Hicham Harhar ◽  
Abdelkader Zarrouk ◽  
Mohamed Tabyaoui
Keyword(s):  
Antioxidant Activity ◽  
Central Composite Design ◽  
Principal Component ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Opuntia Ficus Indica ◽  
Optimal Value ◽  
Total Flavonoids Content ◽  
The One ◽  
Central Composite

Abstract The effect of roasting conditions on antioxidant activity Opuntia Ficus Indica seeds from Morocco and their phenolic compounds were optimized by the Central Composite Design (CDD) method. The CCD was used to optimize the roasting conditions: temperature (X1: 60–200 °C) and roasting time (X2: 10–50 min). The best roasting conditions were used in order to optimize the optimal value of the response : TPC (Total Phenolic Content): X1: 200°C, X2: 50min with 104.86±1.94GAE/g extract predicted response, TFC (Total Flavonoids Content) : X1: 200 °C, X2: 50 min with 81.23±0.90 mg QE/g extract predicted responses, TTC (Total Condensed Tannins Content): X1: 128.9°C, X2: 34.92min with 6.12±0.95 mg QAE/g extract) predicted response. Moreover, the optimal potential antioxidant activity by ABTS assay and DPPH assay was found in extracts of Opuntia Ficus Indica seeds roasted: at a high temperature of the roasting. Furthermore, the positive significant correlations were determined by the principal component analysis (PCA) on the one hand, between the antioxidant capacity of the extracts and their antioxidants compounds (TPC and TFC), and on the other hand, between the two assays ABTS and DPPH. Consequently, the results of this work indicated that roasted Opuntia Ficus Indica seeds from Morocco can be considered as an essential ingredient for human foods.

scholarly journals The Influence of the Variety, Vineyard, and Vintage on the Romanian White Wines Quality

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Anamaria Hosu ◽  
Veronica Floare-Avram ◽  
Dana Alina Magdas ◽  
Ioana Feher ◽  
Mihai Inceu ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Isotopic Ratio ◽  
Principal Component ◽  
Major Influence ◽  
Total Phenolic ◽  
White Wines ◽  
Total Sugars ◽  
Total Flavonoids Content ◽  
Chemometric Techniques ◽  
Different Characteristics

The wine is one of the most consumed drinks over the world, being subjected to falsification or adulteration regarding the variety, vintage, and geographical region. In this study, the influence of different characteristics of wines (type, production year, and origin) on the total phenolic content, total flavonoids content, antioxidant activity, total sugars content, pH, and18O/16O isotopic ratio was investigated. The differentiation of selected wines on the basis of tested parameters was investigated using chemometric techniques, such as analysis of variance, cluster analysis, and principal component analysis. The experimental results are in agreement with other outcomes and allow concluding that variety and vineyard have the major influence on the studied parameters, but, also, statistical interaction effect between year and vineyard and year and variety is observed in some cases. The obtained results have demonstrated that these parameters together with chemometric techniques show a significant potential to be used for discrimination of white wines.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Discrimination of Myrtle Ecotypes from Different Geographic Areas According to Their Morphological Characteristics and Anthocyanins Composition

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 328 ◽  
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Estrella Espada-Bellido ◽  
Marta Ferreiro-González ◽  
Antonio Amores-Arrocha ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fruit Size ◽  
Morphological Characteristics ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Chemical Traits ◽  
Positive Effect

Myrtus communis L. is an evergreen shrub that produces berries with a high content in antioxidant compounds. Since these compounds have demonstrated a positive effect on human health, the interest on berries and their usages has increased. However, environmental conditions may affect the productivity of these species and consequently the quality of wild myrtle. Ecotypes from diverse geographical origins may result in significant variations in terms of bioactive compounds content as well as in chemical traits. For this reason, in this work ecotypes from two different localizations have been studied to determine if their differences in morphological and anthocyanins traits can be attributed to their origin and the environmental characteristics of these locations. For this, chemometric analyses such as Hierarchical Cluster Analysis and Principal Component Analysis, were employed. The results showed differences between the ecotypes depending on their location. In particular, myrtle berries from maritime zones present greater fruit size and amount of bioactive compounds, which means an improvement in the quality of the final product based on this raw material. It can be concluded that both morphological and anthocyanins traits are related to the location of the ecotype and allow selecting the best ecotype for the required applications.

scholarly journals Influence of Pasteurization on Total Phenols Content and Antioxidant Capacity of Prunus persica L. Juices

2017 ◽  
Vol 45 (2) ◽  
pp. 553-560 ◽  
Author(s):  
Vasile LASLO ◽  
Alin C. TEUSDEA ◽  
Sonia A. SOCACI ◽  
Daniel MIERLITA ◽  
Simona I. VICAS
Keyword(s):  
Antioxidant Capacity ◽  
Prunus Persica ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Quality Parameters ◽  
Point Of View ◽  
Total Phenolic ◽  
Total Phenols ◽  
The European Union ◽  
Important Place

Peach and nectarine (Prunus persica ) production has an important place in the world, being the most important fruit after apple crops in the European Union. Because the fruits are perishable, it is desirable to valorize them as juice. Seven peaches and three nectarines cultivars grown in the N-W part of Romania were investigated for quality parameters, volatile profile, total phenols content and antioxidant capacity. The volatile composition of peach and nectarine cultivars was determined via the ITEX/GC-MS technique, the main volatile compounds belonging to alcohols and aldehydes. Another objective was to obtain the pasteurised juices from these fruits and to investigate the best time of pasteurisation in order to identify the most valuable cultivar from the perspective of total phenols content and its antioxidant capacity. For a better interpretation of results and a proper discrimination between cultivars, according to the total phenols content and antioxidant capacity, the multivariate analysis, Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were applied. The peach cultivars showed the highest content in total phenols compared with nectarine. From peach cultivars, the highest concentration was recorded in ‘Southland’ (47.49 ± 0.14 mg GAE 100 g-1 FW) and from nectarine cultivars in ‘Romamer’ (16.28 ± 0.83 mg GAE 100 g-1 FW). The highest antioxidant capacities were recorded in ‘Southland’ peach in the case of both methods (DPPH and FRAP). The results showed that ‘Southland’ peach and ‘Romamer’ nectarine pasteurised juices are the best from the point of view of total phenolic compounds content with high antioxidant capacity.

Bioactive compounds by UPLC-PDA in different cocoa clones (Theobroma cacao L.) developed in the Southern region of Bahia, Brazil

2017 ◽  
Vol 119 (9) ◽  
pp. 2117-2127 ◽  
Author(s):  
Leonardo Fonseca Maciel ◽  
Ana Lúcia de Souza Madureira Felício ◽  
Elisa Yoko Hirooka
Keyword(s):  
Bioactive Compounds ◽  
Phenolic Content ◽  
Principal Component ◽  
Bioactive Compound ◽  
Ultra Performance Liquid Chromatography ◽  
Southern Region ◽  
Cocoa Bean ◽  
Total Phenolic ◽  
Array Detector ◽  
Content Type

Purpose Many factors can influence the bioactive compounds in cocoa beans. The purpose of this paper is to characterize the bioactive compound profile of 12 cocoa bean clones produced in Bahia’s Southern region. Design/methodology/approach Total phenolic content, total flavonoids and total anthocyanins were determined using spectrophotometric methods. Caffeic acid, caffeine, theobromine, catechin and epicatechin contents were determined using Ultra Performance Liquid Chromatography with a Photodiode Array Detector. Principal component analysis (PCA) was carried out using to determine the variability of the results found in relation to the clones studied. Findings The authors observed variations in the bioactive compound content between the analyzed cocoa clones. The SRN clone showed the highest levels of phenolic content, flavonoids, caffeine, catechin, and epicatechin. PCA showed that all the clones had unique characteristics related to the composition of their bioactive compounds content, classifying each clone in a well-defined manner. Originality/value This work is an important contribution for the characterization of new cocoa bean clones in relation to their content of bioactive compounds. The information reported in this paper can be decisive in the choice of the variety of cocoa in the production of chocolate with high bioactive compounds content and claim of functional property.

scholarly journals Variation of Bioactive Compounds and Antioxidant Activity of Jujube (Ziziphus jujuba) Fruits at Different Stages of Ripening

2018 ◽  
Vol 46 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Gheorghe ACHIM ◽  
Mihai BOTU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ripening Stage ◽  
Total Flavonoids Content ◽  
Ziziphus Jujuba

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100 g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human’s healthy eating.

scholarly journals Physical, physicochemical, microbiological, and bioactive compounds stability of low-calorie orange jellies during storage: packaging effect

2020 ◽  
Vol 9 (9) ◽  
pp. e759997900
Author(s):  
Hellen Vidal Santos ◽  
Cassandra Justina Souza Maia ◽  
Elismara de Jesus Ferreira Lima ◽  
Ana Clara Costa Dias ◽  
Reginaldo de Souza Monteiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Total Sugars ◽  
Low Calorie ◽  
Great Progress ◽  
Useful Life ◽  
Compounds Stability ◽  
Growth Of Fungi

Fruit jellies are widely produced as a way to utilize fresh fruits, which are highly perishable. Orange a fruit widely consumed in Brazil, it has a significant amount of bioactive compounds. Despite the great progress in the development of jellies, several factors can change its useful life, among them is the packaging. Therefore, the objective of this study was to evaluate the effect of packaging on the physicochemical, physical, microbiological and bioactive compounds stability of low-calorie orange jellies during storage. Analyses every 30 days during the 180 days of storage. The results showed that increased storage time led to a decrease in pH, reduction of the flow rate (polypropylene packaging), reduction in yellow intensity, and growth of fungi and yeasts (higher in polypropylene packaging). In contrast, luminosity, red intensity, moisture, total sugars, and the consistency index tended to remain stable during storage. The DPPH results showed an increase in the antioxidant activity and reduction of vitamin C throughout the period of storage, especially in polypropylene packaging. The total phenolic content was stable with a tendency to decrease during storage. Notably, vitamin C showed a positive correlation with antioxidant activity in jellies. Low-calorie orange jellies packaged in glass showed the least changes during storage.

scholarly journals Polymorphism analysis in some Algerian Opuntia species using morphological and phenological UPOV descriptors

2017 ◽  
Vol 95 (3) ◽  
pp. 391 ◽  
Author(s):  
Boubakr Hadjkouider ◽  
Ammar Boutekrabt ◽  
Bahia Lallouche ◽  
Salim Lamine ◽  
Néjia Zoghlami
Keyword(s):  
Cluster Analysis ◽  
Hierarchical Cluster Analysis ◽  
Phenotypic Diversity ◽  
Principal Component ◽  
Morphological Diversity ◽  
Hierarchical Cluster ◽  
Relative Magnitude ◽  
Polymorphism Analysis ◽  
Opuntia Ficus Indica ◽  
New Varieties

<p><strong>Background: </strong>In the present study, we have investigated the morphological variation in a set of five <em>Opuntia</em> species from the Algerian steppes using 49 UPOV descriptors.</p><p><strong>Questions: </strong>which of the 49 descriptors that can be used as powerful estimators of the phenotypic diversity within <em>Opuntia</em> species? How is the morphological diversity patterned in Algerian <em>Opuntia</em>?</p><p><strong>Species study/ Mathematical model: </strong><em>Opuntia ficus-indica, Opuntia amycleae, Opuntia streptacantha, Opuntia engelmannii, Opuntia robusta</em><strong>.</strong> Principal Components Analysis (PCA) and Hierarchical Cluster Analysis were used.</p><p><strong>Study site: </strong>Four counties were studied located in the Algerian steppes. The present research was carried out during 2014.</p><p><strong>Methods:</strong> 49 descriptors adopted by the International Union for the Protection of New Varieties of Plants (UPOV) were employed in the present research, where cladode, flower and fruit traits were used to determine the overall degree of polymorphism among 5 <em>Opuntia</em> species.</p><p><strong>Results:</strong> Principal Component Analysis and Hierarchical Cluster Analysis indicated a consistent differentiation between all studied species. The relative magnitude of the first two PCA eigenvectors showed that 8 descriptors out of 49 were identified as the most important descriptors for the classification of the species. The dendrogram performed on the calculated Euclidean distances between all species pairs allowed the identification of 3 groups, unlike the PCA that identified 4 groups. The species <em>Opuntia ficus-indica </em>and <em>Opuntia amycleae</em> were identified as very close morphologically.</p><p><strong>Conclusions: </strong>The present outcome represents a paramount step towards the fast selection of interesting species and for their best management and conservation.</p>

scholarly journals Nutritional Composition, Total Phenolic Content, Antioxidant and α-Amylase Inhibitory Activities of Different Fractions of Selected Wild Edible Plants

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 203 ◽  
Author(s):  
Ziaul Hasan Rana ◽  
Mohammad Khairul Alam ◽  
Mohammad Akhtaruzzaman
Keyword(s):  
Principal Component ◽  
Nutritional Composition ◽  
Wild Plants ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Leaf Extracts ◽  
Data Set ◽  
Antioxidant Power ◽  
High Fiber

Wild plants are considered the richest source of essential nutrients and other beneficial phytochemicals. Hence, the objective of this study was to evaluate the nutritional composition, antioxidant- and α-amylase inhibition activities of leaves and roots of selected Bangladeshi wild plants. These wild plants were found to have high fiber (13.78–22.26 g/100 g), protein (7.08–21.56 g/100 g) and ash (8.21–21.43 g/100 g) contents. The total phenolic and total flavonoid contents were significantly higher in the leaves than the roots. Additionally, antioxidant activity was evaluated using ferric-reducing antioxidant power, 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) and trolox equivalent antioxidant capacity assays and was strongly correlated with phenolic compounds. The leaf extracts of the selected plants also exhibited potent α-amylase inhibition (~71%) and were significantly higher than their root counterparts. Thus, the study findings concluded that the investigated plants were good sources of fiber, protein, mineral, natural antioxidant compounds and α-amylase inhibitors, and their increased intake could provide health benefits. The principal component analysis (PCA) of analyzed variables divided the samples into three clear groups, and the first two principal components accounted for 86.05% of the total data set variance.

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