scholarly journals Investigating the Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus Lam. (Jackfruit) Latex

2021 ◽  
Vol 12 (3) ◽  
pp. 3019-3033

Plant latex is well-established as a defensive exudate against natural herbivory. However, its bioactive properties and potential use as a clinical adjunct are not fully explored in the current literature. In this study, latex was procured from Artocarpus heterophyllus Lam. (jackfruit) and subsequently evaluated for its antioxidant and antimicrobial activity. Aqueous extract (AE) and trifluoroethanol extract (TFE) of jackfruit latex were prepared using distilled water and trifluoroethanol. Both extracts were then characterized via thin-layer chromatography (TLC), ultraviolet-visible (UV-Vis) spectroscopy, and gas chromatography-mass spectrometry (GC-MS) analysis. Antimicrobial activity and antioxidant activity were determined for the extracts. Antibacterial activity was exhibited by TFE against P. aeruginosa, S. aureus, and Bacillus sp. The antioxidant activity of AE was significantly higher than TFE.

2018 ◽  
Vol 5 (2) ◽  
pp. 123
Author(s):  
Asriani Hasanuddin ◽  
Chairil Anwar ◽  
Marhawati Mappatoba ◽  
Hafsah Hafsah

Cocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives.


Author(s):  
Mukesh S Sikarwar ◽  
Chew Khai Szeek ◽  
Neeraj Paliwal

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.


2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity


2017 ◽  
Vol 82 (5) ◽  
pp. 495-508 ◽  
Author(s):  
Aleksandra Bozic ◽  
Nenad Filipovic ◽  
Irena Novakovic ◽  
Snezana Bjelogrlic ◽  
Jasmina Nikolic ◽  
...  

Fourteen mono- and bis-carbohydrazone ligands have been synthesized and characterized. Antioxidant activity of the substances was investigated together with possible (E)/(Z) isomerization, and explained on the most active antioxidant compound 4 in various dimethyl sulphoxide?water mixtures. The addition of water to the system was involved in the formation of hydrated molecules which was confirmed in NMR after the addition of D2O. The ligands were tested in vitro against Gram-positive and Gram-negative bacteria and fungi, and their activity was discussed in relation to the structure of investigated carbohydrazone.


2020 ◽  
Vol 11 (4) ◽  
pp. 2969-2977 ◽  
Author(s):  
Shirley L. Sampaio ◽  
Ângela Fernandes ◽  
Carla Pereira ◽  
Ricardo C. Calhelha ◽  
Marina Sokovic ◽  
...  

Quinoa is a very interesting food due to its nutritional and chemical composition, as well as its bioactive properties, such as antioxidant and antimicrobial activity.


2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Esther Ramírez-Moreno ◽  
Raquel Cariño-Cortés ◽  
Nelly del Socorro Cruz-Cansino ◽  
Luis Delgado-Olivares ◽  
José Alberto Ariza-Ortega ◽  
...  

Seed oils from two Mexican varieties of cactus pear (green: Opuntia albicarpa and red: Opuntia ficus indica) were extracted with different solvents (hexane, ethanol, and ethyl acetate) to evaluate their antioxidant activity. The seed oil with higher antioxidant activity was selected to evaluate antimicrobial activity. The fatty acid profile was analyzed by gas chromatography-mass spectrometry (GC-MS). Oil from green cactus pear seeds obtained with ethanol and ethyl acetate exhibited higher antioxidant activity (p<0.05) of 323 and 316 μmol TE/20 mg (p < 0.05), respectively, compared to red cactus pear seed oil (≈274 and 247 μmol TE/20 mg with ethyl acetate and ethanol, resp.). The oil obtained with ethanol and higher antioxidant activity was used to determine the antimicrobial activity. Both cactus pear oils produced a microbial inhibition zone in most of the microorganisms evaluated, particularly Saccharomyces cerevisiae which had similar diameter (38–40 mm). The oil fatty acids profiles of both varieties were similar and exhibited a high content of linoleic acid, while two fatty acids (linolenic and behenic) found in red cactus pear were not observed in the green variety.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Shabnam Ansari ◽  
Mohammad Maaz ◽  
Iftekhar Ahmad ◽  
Syed Kazim Hasan ◽  
Sajad Ahmad Bhat ◽  
...  

AbstractObjectivesSaussurea lappa, CB Clarke (S.lappa) is a perennial herb of the Compositae family. The root of S.lappa has been used for the treatment of various diseases such as hepatitis, jaundice, intestinal worms, bronchial asthma, and a variety of skin diseases. The aim of the study was to ensure quality control of S.lappa and its preparation HAESL (hydroalcoholic extract of S.lappa) along with assessment of HAESL antimicrobial and antioxidant activities in vitro.MethodsHAESL was prepared with 50% ethanol, (v/v). Physiochemical analysis of the root of S.lappa, and phytochemical screening, thin-layer chromatography (TLC), high-performance thin-layer chromatography (HPTLC), and in vitro antimicrobial and antioxidant activity of HAESL were performed using the standard protocol.ResultsPhysiochemical and phytochemical assessments of S.lappa and HAESL showed the greater quality of the drug. HAESL showed the presence of many phytochemical constituents corresponding to colorful spots, peaks, area under the curve, and corresponding Rf values as evident in the TLC and HPTLC analysis. HAESL showed a concentration-dependent effect on radical scavenging activity against DPHH (2,2-diphenyl-1-picrylhydrazyl) and hydroxyl radical with IC50 value of 19.10 μg/mL and 82.23 μg/mL, respectively. HAESL exhibited antimicrobial activity against the growth of micro-organisms such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Aspergillus flavus, and Candida albicans. HASEL showed no antimicrobial activity against Aspergillus niger.ConclusionsThe procured and an identified sample of Saussurea lappa was good in quality and the prepared HAESL contained many phytochemical compounds. HAESL substantiated significant antioxidant and antimicrobial activity in vitro.


2020 ◽  
Vol 9 (3) ◽  
pp. e119932580
Author(s):  
Edilayane da Nóbrega Santos ◽  
Erick dos Anjos Bezerra ◽  
Jeniffer Viviany dos Santos Fonseca ◽  
Mychelle de Lira Andrade ◽  
João Vitor Fonseca Feitoza ◽  
...  

The bioactive properties of unconventional fruits have been of interest in recent research. The objective of this study was to evaluate the bioactivity of Terminalia catappa Linn fruit and seed through its antioxidant and antimicrobial activity against pathogenic microorganisms. The fruit and the seed were analyzed for the physical-chemical parameters of pH, aw, humidity, ashes, carbohydrates, proteins and lipids. After drying, pulp and almond flours were evaluated for bioactivity (total phenolics, tannins, anthocyanins and flavonoids and antioxidant-DPPH) and antimicrobial activity. The fruit had a higher carbohydrate content (15.65%), while the seed was characterized by high lipid content (48.52% ± 0.31) and protein (26.40% ± 0.10). The pulp flour showed significant amounts of tannins (1662.68 mg.100-1 g ± 1.27) and antimicrobial activity inhibiting Staphylococcus aureus, Pseudomonas putida, Pseudomonas aeruginosa, Clostridium perfringens and Listeria monocytogenes. The seed flour presented antioxidant activity (46.05 mg.100-1 g ± 0.23), but did not present inhibitory power against the tested bacterial strains. Terminalia catappa Linn fruit can be an alternative new ingredient with natural antioxidant action.


2007 ◽  
pp. 355-368 ◽  
Author(s):  
G. Schmourlo ◽  
Z.B. de Morais-Filho ◽  
D.B. de Oliveira ◽  
S.S. Costa ◽  
R.R. Mendonça-Filho ◽  
...  

Author(s):  
Srividya A.R. ◽  
A Shalom ◽  
Chandrasekhar R ◽  
Vijayan P ◽  
V.J Vishnuvarthtan

Polygonum chinensis linn belongs to the family Polygonaceae. The plant used in this study was shade dried and extracted with methanol by a cold maceration process. Qualitiave phytochemicals estimation showed the presence of the majority of compounds including terpinoids, flavonoids, tannins, saponins and glycosides. All the extracts showed moderate inhibition ranged from 12- 14 mm against both Gram-positive and Gram-negative strains and 00-21 mm against fungal trains.  In the two-fold serial dilution method, the minimum inhibitory concentration of the extract ranges between 250 – >1000 µg/ml.  The leaf extract of polygonum chinensis showed the potent activity with an IC50 value of 18.59 ± 0.356 µg/ml. In DPPH, the lipid peroxidation and hydrogen peroxide method, the leaf extract showed potent antioxidant activity. Root and stem extract showed moderate antioxidant activity. All the extracts showed moderate cytotoxicity to all the five cell lines Vero, A-549, L6, BRL, 3A and L-929 with CTC50 value ranging from 400-125 µg/ml. Short-term cytotoxicity studies were conducted for all the extracts against (Dalton Lymphocytes ascites) cells.  The CTC50 value was found to be 435 µg/ml. Thus, the extracts of P. Chinensis may possess cytotoxic, antioxidant and antimicrobial activity.   


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