scholarly journals PEMBUATAN NANOPARTIKEL-PERAK EKSTRAK DAUN UBI JALAR ORANGE (Ipomoea batatas L.) DENGAN METODE BIOREDUKSI DAN UJI AKTIVITAS TERHADAP JUMLAH TROMBOSIT Mus musculus

Alotrop ◽  
2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Ririn Trinanda ◽  
Agus Sundaryono ◽  
Dewi Handayani

This study aims to make silver nanoparticles (NPP) of orange Sweet potato extract (Ipomoea batatas L.) using bioreduction method and test the effect of giving silver nanoparticles - leaf extract of Ipomoea batatas L. orange (Ipomoea batatas L.) to the number of thrombocyte from male of  Mus musculus induced by aspirin. The precursor used is 1 mM AgNO3 solution and as bioreductor is an antioxidant compound in orange sweet potato extract (Ipomoea batatas L.). The ratio of AgNO3 1 mM solution volume and orange sweet potato leaf extract is 9: 1 with 2 hours time. The characteristic test again obtained NPP is done using FTIR and Particle Size Analyzer (PSA) using Dynamyc Light Scattering method (DLS). The results showed that from the PSA analysis is known the average size of NPP – I. Batatas, L leaf extract  is an average of 86.0 nm with a polydispersity value (PDI) of 0.17 < 1 that indicates that the  NPP-leaf extract particle size distribution It is less homogeneous and are less stable.The results show that the administration of silver nanoparticles - orange sweet potato extract (Ipomoea batatas L.) can increase the platelet count of male mice significantly Dose of 0.014 g / Kgbb of 259,600 / mm3. 

2020 ◽  
Vol 9 (4) ◽  
pp. 438
Author(s):  
Ade Maria Kristin Gultom ◽  
Ni Made Yusa ◽  
Anak Agung Istri Sri Wiadnyani

This study aims to determine the effect of solvent types on antioxidant activity of white sweet potato leaf extract (Ipomoea batatas L) and to obtain the most appropriate type of solvent to produce white sweet potato leaf extract with high antioxidant activity. The experimental design uses in this research was Completely Randomized Design (CRD) with the treatment of solvent types methanol 80%, acetone 80%, ethanol 80% and aquades. The treatment was repeated four times so obtain 16 units of the experimental. Data were analysis of variance and followed by Duncan test if the treatment has a significant effect on measured variable. The results showed that, type of solvent had an obvious effect on antioxidant activity of white sweet potato leaves. Methanol was the best solvent in extract white sweet potato leaves with followed by yield was 32.11%, total flavonoids was 226.45 mg QE / g, total tannin was 16.58 mg TAE / g, vitamin C was 119.42 mg AAE / g and antioxidant activity was 82.42%.


2020 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Nur Rizki ◽  
Muhammad Sugihartono ◽  
Muarofah Ghofur

Use of sweet potato leaf extract to determine the blood glucose response of jelawat fish       (L. hoeveni, Blkr)  seeds maintained in the media.This study aims to determine the optimal concentration of sweet potato leaf extract to reduce the stress level of Jelawat fish (L. hoeveni, Blkr) seeds by measuring blood glucose. In this study, four treatments were used with three replications using a concentration of 2ml / L sweet potato leaf extract, 4ml / L extract concentration, 6ml / L extract concentration, and control. The results showed that the 2ml / L extract concentration was the best because it was proven that the fish blood glucose response at every 1st, 6th, 12th hour, 24th hour, 168th hour fish (L. hoeveni, Blkr)  seed blood glucose levels nettle nearly close to normal blood glucose levels in fish.


2022 ◽  
Vol 20 (2) ◽  
pp. 301-308
Author(s):  
Hyun Kang ◽  
Sung-Gyu Lee

Purpose: To investigate the hepatoprotective effect of Ipomoea batatas extract against alcohol-induced liver damage in mice. Methods: Male C57BL/6 mice were randomly divided into 4 experimental groups (n = 10). Normal Group: The animals received distilled water 5 ml/kg for 7 days; Alcohol Group: The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Purple sweet potato leaf extract (PSPE) Group: PSPE 400 mg/kg was for 7 days. The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Hovenia dulcis Thunb extract (HDE) Group: HDE 400 mg/kg was for 7 days. To confirmed to the liver protection effect of PSPE, it was calculated, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol (TC) in serum were detected. To evaluate changes of histological in alcohol-fed mice, liver tissue was determined by H&E staining. Results: Blood alcohol concentration in purple sweet potato leaf extract (PSPE) 200 mg/kg and Hovenia dulcis (H. dulcis) extract (HDE) 200 mg/kg treated group significantly decreased compared to - alcohol with water treated group (p < 0.05). Serum ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced. Liver sections in mice stained with H&E (hematoxylin and eosin) stain to displayed the physiological changes in the liver tissue. Furthermore, the results showed that inflammatory cells increased in the alcohol group compared to the normal group, but spontaneously decreased in the PSPE or HDE-treated group. Conclusion: These results demonstrate that Ipomoea batatas may be therapeutically effective in protecting the liver from alcohol-induced hepatotoxicity and fatty liver.


Alotrop ◽  
2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Ade Indriani Putri ◽  
Agus Sundaryono ◽  
I Nyoman Chandra

The purpose of this study is to determine and measure the results of the characterization of Chitosan nanoparticle synthetized from leaves of sweet potato (Ipomoea batatas l.) extracts . The methods used to make nanoparticles is made with Ionic Gelation method based on electrostatic interaction between the Hydroxyl Amine on Chitosan with cluster of negative charge from the polianion NaTPP with conditioned surfactant Tween 80 used as surfactant. Characterization test of nanoparticle morphology in this study obtained using the Fourier Transformation  Infra Red (FTIR), and Particle Size Analyzer (PSA). Results with FTIR characterization test from the obtained Chitosan-white purple sweet potato leaf extract nanoparticles has a cluster of N-H and P = O that show the occurrence of ammonium ion interaction between Chitosan with polianion from NaTPP and sweet potato leaves. The interaction seen from wave number and intensities  O-H shifting from pure Chitosan at 3425.56 cm-1 to 3427, 51 cm-1, as well as on pure Chitosan N-H absorption  undergoes a shift from 1597, 06 cm-1 to becomes 1629.85-1 cm-1, which shows that there has been a cross connective between ammonium ion on Chitosan and Posphat from NaTPP and white purple sweet potato leaf extract. The results of the analysis from the PSA, is known to the average Nanokitosan particle sizes extracts from white purple sweet potato leaf extracts with the addition of NaTPP i.e. of 302.6 nm and that the aim of the research on Chitosan nanoparticle synthesis has been successfully done.


2021 ◽  
Vol 4 (01) ◽  
pp. 59-71
Author(s):  
Siti Hamidatul Aliyah ◽  
Desi Sagita ◽  
Devi Kurnia

Abstract   Background: Purple sweet potato leaves (Ipomoea batatas L.) which has been known as immunomodulatory activity, which contains chemical compounds, such as vitamins, minerals, flavonoids (anthocyanin), especially iron found in a higher proportion in the leaves. Therefore, it is necessary to study the immunomodulatory activity of the leaf extract of purple sweet potato (Ipomoea batatas L.) on the immune response in Balb/c mice who induced by hepatitis B vaccines. Method: Inducing performed at day 0 and day 15. Mice with weights ± 20 g, which has been divided into normal control group, I, II, III, IV and V (each 5 mice), respectively were given orally by the leaf extract of purple sweet potato (Ipomoea batatas L.) (doses 100 mg / kg, 200 mg / kg, 300 mg / kg, 400 mg / kg and 500 mg / kg), normal controls (CMC Na 0,5%). With the volume of giving 1% of body weight in mice, performed during the study (20th days). Blood sampling is done via the tail vein (laeralis vein), to calculate the number of leukocytes (21st days) and lymph decision to count lymphocytes (21st days). Results: The results that the leaf extract of purple sweet potato (ipomoea batatas L.), especially a dose of 500 mg / kg as immunomodulatory activity can increase the number of lymphocytes proliferation cell. Conclusion: Purple sweet potato leaf extract has activity against the increase in the number of lymphocytes cell especially on dose 500 mg/kg BW who induced by hepatitis B vaccine that can stimulated an immune response.      


2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins


Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 103
Author(s):  
Jin Mark D. G. Pagulayan ◽  
Aprille Suzette V. Mendoza ◽  
Fredelyn S. Gascon ◽  
Jan Carlo C. Aningat ◽  
Abigail S. Rustia ◽  
...  

The study aimed to evaluate the effects of process parameters (time and raw material weight (RMW)) of conventional (boiling for 10–45 min) and microwave-assisted (2–8 min) aqueous extraction on the color quality (i.e., lightness (L*), chroma (C*), and hue (H°) of anthocyanin –based colorants of red and Inubi sweet potato (Ipomoea batatas L.) leaves. Using response surface methodology, it was found that RMW and boiling time (BT) and microwave time (MT) generally had a significant (p < 0.05) effect on the color quality of the extract from both extraction methods. The effects were found to vary depending on the extraction method and variety of the leaves used. Both extraction methods produced a brown to brick-red extract from the Inubi variety that turned red-violet to pink when acidified. The red sweet potato leaves produced a deep violet colored extract that also turned red-violet when acidified. It is recommended that the anthocyanin content of the extracts be measured to validate the impact of the methods on the active agent. Nevertheless, the outcomes in this study may serve as baseline data for further studies on the potential of sweet potato leaf colorants (SPLC) as a colorant with functional properties.


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