scholarly journals ANALISIS KADAR MERKURI PADA BIOTA AIR DENGAN NANOPARTIKEL PERAK SECARA CITRA DIGITAL DI LOKASI PENAMBANGAN EMAS KABUPATEN LEBONG

Alotrop ◽  
2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Hendry Nordan ◽  
Mochamad Lutfi Firdaus ◽  
Rina Elvia
Keyword(s):  
Limit Of Detection ◽  
Mercury Level ◽  
Digital Camera ◽  
Aquatic Biota ◽  
Digital Imagery ◽  
Lod Score ◽  
Analysis Technique ◽  
Fish Samples ◽  
Biota Samples ◽  
River Fish

This study aimed at determining of how the sensitivity of silver nanoparticles (NPP) in detecting metal mercury in aquatic biota samples through digital imagery. The Sampling of aquatic biota was carried out in the gold mining location of Lebong Tambang village in Lebong district (102 ° 12'00 "-102 ° 18'05" BT and 3 ° 10'00 "-3 ° 17 ' 00 "LS.) The aquatic biota samples analyzed included fish, shellfish, shrimp and plants as well as comparison samples such as water and sediment. The Analysis of mercury level was carried out from December 2018 - March 2019 by using NPP of digital imagery method. The digital imagery method was used as a detector to replace the conventional spectrophotometer. The result of mercury level in aquatic biota with NPP in digital imagery was susceptible compared to the UV-Vis spectrophotometry method. It can be seen from the Limit Of Detection (LOD) score of the digital imagery method with the SLR data analysis technique by using a digital camera that is equal to 2.305 ppb, where the score was smaller than the LOD value in spectrophotometry which is 300 ppb. The results of the analysis of mercury level by using digital imagery method were obtained the concentration on pool shells of 196.8 ppb, in pond fish samples of 155.7 ppb, in shrimp samples of 81.2 ppb, in river fish samples of 81.1 ppb, and in plant samples of 50.9 ppb. thus, these results indicate that the presence of mercury ions in the samples of aquatic biota tested has levels above the threshold, which means samples of fish, shrimp, shellfish and plants were risk to be consumed

scholarly journals Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Duygu Çimen ◽  
Nilay Bereli ◽  
Adil Denizli
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Kinetic Studies ◽  
Force Microscopy ◽  
Spr Sensor ◽  
Atomic Force ◽  
Analysis Technique ◽  
Molecular Imprinting Technology

In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.

scholarly journals Revisiting the Neuroblastoma Cell-Based Assay (CBA-N2a) for the Improved Detection of Marine Toxins Active on Voltage Gated Sodium Channels (VGSCs)

Toxins ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 281 ◽  
Author(s):  
Jérôme Viallon ◽  
Mireille Chinain ◽  
Hélène Taiana Darius
Keyword(s):  
High Throughput Screening ◽  
Matrix Effects ◽  
Limit Of Detection ◽  
Neuroblastoma Cell ◽  
Consensus Protocol ◽  
Limit Of Quantification ◽  
Voltage Gated ◽  
Starting Point ◽  
Fish Samples ◽  
Seafood Products

The neuroblastoma cell-based assay (CBA-N2a) is widely used for the detection of marine biotoxins in seafood products, yet a consensus protocol is still lacking. In this study, six key parameters of CBA-N2a were revisited: cell seeding densities, cell layer viability after 26 h growth, MTT incubation time, Ouabain and Veratridine treatment and solvent and matrix effects. A step-by-step protocol was defined identifying five viability controls for the validation of CBA-N2a results. Specific detection of two voltage gated sodium channel activators, pacific ciguatoxin (P-CTX3C) and brevetoxin (PbTx3) and two inhibitors, saxitoxin (STX) and decarbamoylsaxitoxin (dc-STX) was achieved, with EC50 values of 1.7 ± 0.35 pg/mL, 5.8 ± 0.9 ng/mL, 3 ± 0.5 ng/mL and 15.8 ± 3 ng/mL, respectively. When applied to the detection of ciguatoxin (CTX)-like toxicity in fish samples, limit of detection (LOD) and limit of quantification (LOQ) values were 0.031 ± 0.008 and 0.064 ± 0.016 ng P-CTX3C eq/g of flesh, respectively. Intra and inter-assays comparisons of viability controls, LOD, LOQ and toxicity in fish samples gave coefficients of variation (CVs) ranging from 3% to 29%. This improved test adaptable to either high throughput screening or composite toxicity estimation is a useful starting point for a standardization of the CBA-N2a in the field of marine toxin detection.

Occurrence of Histamine in Canned Fish Samples (Tuna, Sardine, Kilka, and Mackerel) from Markets in Tehran

2019 ◽  
Vol 83 (1) ◽  
pp. 136-141 ◽  
Author(s):  
LEILA PEIVASTEH-ROUDSARI ◽  
ANOSHEH RAHMANI ◽  
NABI SHARIATIFAR ◽  
BEHROUZ TAJDAR-ORANJ ◽  
MANSOOREH MAZAHERI ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Control Point ◽  
Uv Detector ◽  
Histamine Concentration ◽  
Critical Control Point ◽  
Canned Tuna ◽  
Canned Fish ◽  
Relative Standard ◽  
Significant Difference ◽  
Fish Samples

ABSTRACT Food poisoning is one of the most addressed health issues and has raised notable concerns. Histamine is the biogenic amine responsible for scombroid poisoning, which is due to the histidine decarboxylation by bacterial decarboxylases in various types of fish and fish products. The present investigation was conducted to measure the concentration of histamine in canned fish samples of tuna in oil (n = 18), tuna in oil with vegetables (n = 15), tuna in brine (n = 9), kilka in oil (n = 9), sardine in oil (n = 3), and mackerel in oil (n = 6) collected from markets in Tehran, Iran. Histamine concentrations were determined with a high-performance liquid chromatography device equipped with a UV detector. For method validation, the correlation coefficient (R2), recovery percentage, relative standard deviation for repeatability, limit of detection, and limit of quantification were 0.99, 82%, 1.3%, 1.5 mg/kg, and 5 mg/kg, respectively. Histamine was detected in 46.6% of the samples, and 18.3% of samples exceeded the histamine limit stipulated by the U.S. Food and Drug Administration (50 mg/kg). The overall mean histamine concentration was 17.36 ± 15.44 mg/kg, with a range of 0 to 88 mg/kg. A significant difference in histamine concentration was found between canned tuna in oil and canned tuna in brine (P < 0.05). However, no significant difference in histamine concentration was found among samples of canned tuna in brine, canned sardine in oil, canned kilka in oil, and canned mackerel in oil. Because of the high histamine concentrations detected in some brands of Iranian canned tuna, precise control programs, hazard analysis critical control point systems, and good hygiene practices should be implemented. HIGHLIGHTS

scholarly journals INTEGRATION OF GEODATA IN DOCUMENTING CASTLE RUINS

2016 ◽  
Vol XLI-B3 ◽  
pp. 345-349
Author(s):  
P. Delis ◽  
M. Wojtkowska ◽  
P. Nerc ◽  
I. Ewiak ◽  
A. Lada
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Digital Camera ◽  
Terrestrial Laser Scanning ◽  
Point Clouds ◽  
3D Models ◽  
Digital Imagery ◽  
Historical Structures ◽  
Three Dimensional Models ◽  
Different Sources

Textured three dimensional models are currently the one of the standard methods of representing the results of photogrammetric works. A realistic 3D model combines the geometrical relations between the structure’s elements with realistic textures of each of its elements. Data used to create 3D models of structures can be derived from many different sources. The most commonly used tool for documentation purposes, is a digital camera and nowadays terrestrial laser scanning (TLS). Integration of data acquired from different sources allows modelling and visualization of 3D models historical structures. Additional aspect of data integration is possibility of complementing of missing points for example in point clouds. The paper shows the possibility of integrating data from terrestrial laser scanning with digital imagery and an analysis of the accuracy of the presented methods. The paper describes results obtained from raw data consisting of a point cloud measured using terrestrial laser scanning acquired from a Leica ScanStation2 and digital imagery taken using a Kodak DCS Pro 14N camera. The studied structure is the ruins of the Ilza castle in Poland.

scholarly journals ASPEK BIOLOGI IKAN LIDAH (Achiroides leuchorhinchos) DAN SEBARAN DI SUNGAI MUSI, SUMATERA SELATAN

2017 ◽  
Vol 14 (3) ◽  
pp. 273
Author(s):  
Eti Nurhayati ◽  
Eko Prianto
Keyword(s):  
Food Habit ◽  
Dry Season ◽  
Spawning Season ◽  
Fish Samples ◽  
Size Measure ◽  
Negative Growth ◽  
Electric Fishing ◽  
Musi River ◽  
River Fish

Penelitian ini dilakukan di Sungai Musi, Sumatera Selatan pada tahun 2007 untuk mempelajari biologi ikan lidah (Achiroides leuchorhinchos) yang meliputi distribusi ukuran, bobot tubuh, kebiasaan makan, fekunditas, serta sebaran di Sungai Musi. Contoh ikan diperoleh dengan alat jaring, jala, dan stroom eksperimen. Hasil penelitian ini menunjukkan bahwa ikan lidah (Achiroides leuchorhinchos) terdapat di Sungai Musi bagian hulu sampai dengan hilir. Ikan ini memiliki pola pertumbuhan allometrik negatif (b=2.894) dengan ikan terbanyak pada kisaran panjang 41 sampai dengan 48 mm dan 0,5 sampai dengan 1 g. Ikan lidah (Achiroides leuchorhinchos) termasuk ikan karnifora dengan makanan utama kepiting dan makanan pelengkap ikan. Pola pemijahan adalah total spawner dengan fekunditas sekitar 2x103 butir telur berukuran rata-rata 0,06 mm. Musim pemijahan ikan lidah (Achiroides leuchorhinchos) diperkirakan terjadi pada bulan Juli (musim kemarau). Research was done in Musi River, South Sumatera in 2007 to study the biology of tongue fish (Achiroides leuchorhinchos) covering distribution of size measure, body wight, food habit, fecundity, and distribution in Musi River. Fish samples were collected by using net and electric fishing. The result shows that tongue fish (Achiroides leuchorhinchos) was found in upstream until downstream. This fish has negative growth allometric pattern (b=2,894). Tongue fish is carnivore with the espesial food crab and fish as complement food. Its fecunditity pattern is total spawner with number of eggs of about 2x103 eggs, size of eggs of 0.06 mm. The spawning season of tongue fish might occur on July (dry season).

scholarly journals Analysis of Mercury in Fish, North Sumatera Indonesia by Atomic Absorption Spectrophotometer

2020 ◽  
Vol 8 (3) ◽  
pp. 21-25
Author(s):  
Jhan Saberlan Purba Purba ◽  
Jansen Silalahi
Keyword(s):  
Atomic Absorption ◽  
Mercury Level ◽  
Maximum Level ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometer ◽  
Spanish Mackerel ◽  
Fish Samples ◽  
Mercury In Fish ◽  
Aas Method

The maximum level of mercury permitted in fish by BPOM RI is 0.5 mg/kg. This study was conducted to determine the level of mercury in fish often consumed in Medan Indonesia. Fish samples were collected from 7 traditional fish market outlets and 1 supermarket in Medan in December 2019. Fish samples were dried using an oven at 1030C untill a constant dry weight was attained. Then, the digestionprocess was carried out using concentrated nitric acid and perchloric acid. Determination of mercury level was carried out using CV-AAS method with 900 H Perkin Elmer atomic absorption spectrophotometer equipped with MHS-15 (Mercury Hidride System). The results showed that the level of mercury in pelagic fish was found significantly in yellowfin tuna (Thunnusalbacares) of 27.3865 ± 0.3326 µg/kg; mackerel tuna (Euthynnusaffinis) of 17.8570 ± 0.0121 µg/kg; skipjack tuna(Katsuwonuspelamis) of 17.4507 ± 1.5893 µg/kg; spanish mackerel (Scomberomoruscommersonii) of 10.5767 ± 0.1862 µg/kg; grouper (Epinepheusfuscoguttatus) of  9.9736 ± 0.9115 µg/kg; mackerel (Rastreligerkanagurta) of  6.5364 ± 0.1935 µg/kg and dumersal fish were found significantly in red snapper (Lutjanuscampechanus) of 14.0966 ± 0.8555 µg/kg; stingray(Dasyatissp) of 61.3146 ± 0.8149 µg/kg; ariid catfish (Arius thalassinus) of 13.2533 ± 0.7586 µg/kg; black pomfret(Parastromateusniger) of 10.7755 ± 0.4605 µg/kg; sardine(Sardinellasp) of 6.5464 ± 0.1036. The results showed that the larger fish has the higher mercury level. The level of mercury in these analyzed fish samples was below maximum level permitted by BPOM RI.  

Nuclear Activation Techniques for Heavy Metal Pollutants

1970 ◽  
Vol 5 (1) ◽  
pp. 9-33
Author(s):  
R.E. Jervis ◽  
B. Tiefenbach ◽  
W. LePage ◽  
J-L Debrun
Keyword(s):  
Toxic Metals ◽  
Feeding Habits ◽  
Water System ◽  
High Sensitivity ◽  
Mercury Content ◽  
Pollution Level ◽  
Pollution Levels ◽  
Analysis Technique ◽  
Fish Samples

Abstract The sensitive radioactivation analysis technique has considerable scope for application in environmental studies. In this paper the use of the applied nuclear method for the determination of nanogram to microgram quantities of several toxic metals (such as F, As, Se, Cd, Hg and Th) in environmental materials, is reported. In typical analyses, samples 0.05 to 0.5 grams in weight have been irradiated for periods up to 50 hours in the McMaster Reactor at approximately 1013 neutrons/cm2 sec thermal neutron flux. With certain samples the identification and determination of trace metal has been accomplished quickly and with high sensitivity by the use of instrumental or non-destructive g-ray spectrometry, while in certain other samples containing metals at lower concentrations, the analysis was facilitated by the use of a few radiochemical separation steps. The mercury content of fish samples taken from various water bodies in Ontario and elsewhere has been determined in the concentration range from 0.01 to 2 ppm. The levels found have been tentatively related to the particular species of fish and their feeding habits as well as to the pollution level in the water system. Similar pollution levels of Se and As have been determined in areas suspected of industrial pollution, and hair activation analyses have been used to monitor human ingestion of these and other toxic metals. A novel on-stream method for the detection of trace levels of metals such as Th and U is also outlined and its application to water pollutants such as P is discussed.

Twin-Fluid Atomized Spray Combustion of Straight Vegetable Oil at Elevated Pressures

2017 ◽  
Author(s):  
Yonas G. Niguse ◽  
Ajay K. Agrawal
Keyword(s):  
Vegetable Oil ◽  
Soot Formation ◽  
Digital Camera ◽  
Chamber Pressure ◽  
Nox Emissions ◽  
Elevated Pressures ◽  
Straight Vegetable Oil ◽  
Fuel Flow ◽  
Analysis Technique ◽  
Swirl Stabilized Combustion

The effect of the chamber pressure on combustion of a twin-fluid-atomized spray of straight vegetable oil (VO) in a swirl stabilized combustion system is experimentally studied. A system with high pressure capabilities was developed, and flame and emissions characteristics of VO are investigated at elevated pressures, up to about 5 bars, different heat release rates and atomizing air to liquid ratios (ALR) by mass. An image analysis technique was developed to infer flame and soot characteristics from visual images acquired by a digital camera. An increase in the ALR improved combustion characterized by blue flames, lower CO and NOx emissions, and minimal soot formation. For a given fuel flow rate, an increase in the chamber pressure resulted in smaller volume flames with lower CO levels but higher NOx emissions. Compared to diesel, as pressure increased, straight VO flames produced lower NOx and more voluminous flames characterized by distributed combustion with less soot formation. Overall, straight VO could be atomized and combusted at elevated pressures using the twin-fluid atomizer of the present study, and the resulting VO flames exhibited less sensitivity to chamber pressure variations.

scholarly journals Colorimetric hand-held sensors and biosensors with a small digital camera as signal recorder, a review

2020 ◽  
Vol 39 (1) ◽  
pp. 20-30
Author(s):  
Miroslav Pohanka
Keyword(s):  
Analytical Methods ◽  
Limit Of Detection ◽  
Wide Spectrum ◽  
Digital Camera ◽  
Digital Cameras ◽  
Optical Instruments ◽  
Color Density ◽  
Layer Chromatography ◽  
Signal Recorder ◽  
Made In

AbstractSensors, biosensors, lateral flow immunoassays, portable thin-layer chromatography and similar devices for hand-held assay are tools suitable for field or out of laboratories assays of various analytes. The assays frequently exert a limit of detection and sensitivity close to more expensive and elaborative analytical methods. In recent years, huge progress has been made in the field of optical instruments where digital cameras or light sensitive chips serve for the measurement of color density. General availability of cameras, a decrease of prices and their integration into wide spectrum phones, tablets and computers give the promise of easy application of analytical methods where such cameras will be employed. This review summarizes research on hand-held assays where small cameras like the ones integrated into smartphones are used. Discussion about such assays, their practical applicability and relevant specifications are also written here.

scholarly journals Development of the High Sensitivity and Selectivity Method for the Determination of Histamine in Fish and Fish Sauce from Vietnam by UPLC-MS/MS

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Quang Hieu Tran ◽  
Thanh Tan Nguyen ◽  
Kim Phuong Pham
Keyword(s):  
Limit Of Detection ◽  
High Sensitivity ◽  
Fish Sauce ◽  
Limit Of Quantification ◽  
Fish Samples ◽  
The Matrix ◽  
Liquid Chromatography Tandem Mass ◽  
Sensitivity And Selectivity ◽  
Liquid Chromatographic

A selective, sensitive, and rapid method by using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of histamine in fish and fish sauce was developed. The optimal conditions of liquid chromatographic separation and mass spectroscopy of histamine have also been investigated. The linear ranges of the method were 20.0 ÷ 1000 ng/mL, and the corresponding correlation coefficient was 0.9993. Mean recoveries of the analyte at three spike levels (low, medium, and high) were within the range of 98.5% ÷ 102.5% (n = 7). The limit of detection (LOD) and limit of quantification (LOQ) values were 3.83 and 11.50 ng/mL for the fish sauce sample and 4.71 and 14.12 ng/mL for the fish sample, respectively. The influence of the matrix effect on the accuracy, repeatability, and recovery of the method was negligible. The recommended method was applied to determine the content of this substance in 21 fish sauce samples and 4 kinds of fish samples, which were collected from Ho Chi Minh City, Vietnam, in 2019.

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