PENGARUH METODE EKSTRAKSI KENCUR (Kaempferia galanga L.)  TERHADAP PERTUMBUHAN Staphylococcus aureus

Kaempferia galanga L. or known as Kencur is an herbal plant that is widely used by people with various benefits such as influenza in infants, headaches, sprains, relieving fatigue, inflammation of the stomach, coughing, improving menstruation, inflammation of children's ears, dirty blood, eyes aches, diarrhea, and colds. However, studies that have used Kencur as an antibacterial against Staphylococcus aureus have never been done. Therefore, scientific research is needed to determine the ability of Kencur extract as an antibacterial that inhibits the growth of Staphylococcus aureus. 3 extraction methods were used, with the aim of determining the right method for obtaining Kencur bioactive compounds as antibacterial. The three extraction methods used were the press method, the infusion method, and the maceration method using methanol as a solvent. Each extract obtained from various extraction methods will be tested for antibacterial by observing the growth of Staphylococcus aureus. It was found that with the press method there were no clear zones or growth inhibition zones of Staphylococcus aureus. Using the extract from the infusion method, the same thing is obtained, namely there is no clear zone or growth inhibition zone of Staphylococcus aureus. However, it is different when given an extract from the maceration method using methanol solvent, a 17 mm clear zone is formed but there is no inhibition zone. This can indicate that, methanol extract from Kencur can inhibit the growth of Staphylococcus aureus (17 mm).

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PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

Meditory : The Journal of Medical Laboratory ◽

10.33992/m.v5i2.138 ◽

2018 ◽

Vol 5 (2) ◽

Author(s):

Nyoman Mastra

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone ◽

Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone

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ANTIBACTERIAL POTENTIAL OF JAMU INGGU AGAINTS SOME BACTERIA CAUSING UPPER RESPIRATORY TRACT INFECTION

Jurnal Penelitian Saintek ◽

10.21831/jps.v20i2.9595 ◽

2015 ◽

Vol 20 (2) ◽

Author(s):

Nunung Sulistyani ◽

Ardina Nugrahani

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Respiratory Infections ◽

Inhibition Zone ◽

Diffusion Method ◽

Upper Respiratory Tract ◽

Clear Zone ◽

Bacteria Growth ◽

Growth Inhibition Zone ◽

Upper Respiratory

This study aims to examine the potential of jamu inggu as an alternative antibacterial herb that can be used against Staphylococcus aureus, Streptococcus pnuemoniae, S. Pyogenes, and Pseudomonas aeruginosa that is causing Upper Respiratory Infections (URI). Antibacteria activity test of jamu inggu was using the Agar diffusion method by Kirby-Bauer. To test jamu inggu antibacterial activity against Staphylococcus aureus,Streptococcus pnuemoniae, S. Pyogenes, and Pseudomonas aeruginosa, the diameter of the test bacteria growth inhibition zone was measured. The formation of a clear zone around the disc indicates that jamu inggu has chemical compounds that are antibacterial against Staphylococcus aureus,while the Pseudomonas aerugenosa, Streptococcus pyogenes, and S. pneumoniae bacteria cannot be inhibited by jamu inggu. It was indicated by no formation of a clear zone around the disc. Result shows that jamu inggu can be used as an alternativesource of antibiotics against the Staphylococcus aureus

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PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

Meditory : The Journal of Medical Laboratory ◽

10.33992/m.v6i1.227 ◽

2018 ◽

Vol 6 (1) ◽

Author(s):

Nyoman Mastra

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone ◽

The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

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Evaluating the Effects of Peracetic Acid and Chlorine Dioxide Disinfectants on Staphylococcus Aureus Isolated from Surfaces of NICU

Journal of Environmental Health and Sustainable Development ◽

10.18502/jehsd.v4i2.1058 ◽

2019 ◽

Author(s):

Mina Usefi ◽

Hengameh Zandi ◽

Sara Jambarsang ◽

Mehdi Mokhtari ◽

Mahmood Noori Shadkam

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Chlorine Dioxide ◽

Peracetic Acid ◽

Antimicrobial Agents ◽

Inhibition Zone ◽

Health Care Facilities ◽

Diffusion Method ◽

Biochemical Tests ◽

Growth Inhibition Zone

Introduction: Today, disinfection of surfaces by using antimicrobial agents is critical for the prevention and control of pathogens and reduction of infection in hospital. The aim of this study was to investigate and compare the effects of two disinfectants against Staphylococcus aureus isolated from the NICU of Shahid Sadoughi Hospital of Yazd in 2017. Materials and Methods: In this descriptive cross sectional study, bacterial culture of samples collected from different surfaces of the NICU and S. aureus isolates were identified using conventional biochemical tests. Peracetic acid and chlorine dioxide various concentrations were used as disinfectants. Their effects against Staphylococcus aureus were determined by Standard disc diffusion method. Data were analyzed by linear mixed models in SPSS version 23. Results: 39.39% of samples were found to be S. aureus infected. The mean diameter of growth inhibition zone for peracetic acid 0.1% was significantly lower than that for peracetic acid 0.2%, and peracetic acid 0.1% was significantly higher than that for chlorine dioxide (P < 0.001). The comparison of growth inhibition zone diameters for peracetic acid 0.1% and chlorine dioxide disinfectants showed that the average diameter of the inhibition zone created by peracetic acid 0.1% was significantly higher than that created by chlorine dioxide. The most effective disinfectant on S. aureus strains isolated was peracetic acid 0.2% and the least effective disinfectant was chlorine dioxide. Conclusion: In health care facilities with S. aureus infection, peracetic acid 0.2% can be used effectively to reduce nosocomial infection rate.

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Potensi Antibakteri dari Bakteri Berasosiasi Thalassia hemprichii dari Perairan Lemukutan

BULETIN OSEANOGRAFI MARINA ◽

10.14710/buloma.v6i2.16190 ◽

2017 ◽

Vol 6 (2) ◽

pp. 130

Author(s):

Nora Idiawati ◽

Mega Sari Juane Sofiana ◽

Diah Wulandari Rousdy

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Salmonella Typhimurium ◽

Inhibition Zone ◽

Bacterial Strains ◽

Clear Zone ◽

Thalassia Hemprichii ◽

Vibrio Cholera ◽

Growth Inhibition Zone

Thalassia hemprichii merupakan jenis lamun yang ada di perairan Lemukutan. T. hemprichii berpotensi menghasilkan senyawa antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari bakteri berasosiasi lamun T. hemprichii dari perairan Lemukutan. Hasil uji aktivitas antibakteri menunjukkan 6 dari 8 isolat bakteri berasosiasi T. hemprichii aktif terhadap bakteri uji. Aktivitas antibakteri ditunjukkan dengan terbentuknya zona bening di sekitar isolat bakteri berasosiasi T. hemprichii. Isolat bakteri LM07 menunjukkan aktivitas antibakteri yang kuat terhadap 4 bakteri uji. LM07 menunjukkan aktivitas antibakteri terhadap Staphylococcus aureus, Vibrio cholera, Pseudomonas aeruginosa dan Salmonella typhimurium. Thalassia hemprichii is a species of marine seagrass in the Lemukutan waters. It has a potency as a antibacterial source. The aim of the research was to obtained bacteria associated T. hemprichii. The result of this research showed six of eight bacterial strains have antibacterial activity. A growth inhibition zone formed as a clear zone around bacterial strains. LM07 showed the highest antibacterial activity. This bacteria inhibited the growth of S. aureus, V. cholera, P. aeruginosa dan S. typhimurium.

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Influence of base on the release of antibiotics from officinal ointments

Vojnosanitetski pregled ◽

10.2298/vsp0505383t ◽

2005 ◽

Vol 62 (5) ◽

pp. 383-387

Author(s):

Marija Toskic-Radojicic ◽

Zorka Nonkovic

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Lipid Analysis ◽

Inhibition Zone ◽

Aureus Strain ◽

Growth Inhibition Zone ◽

Erythromycin Base ◽

Thermotropic Liquid Crystals ◽

Agar Diffusion Test

Backgraund. Officinal basis for the antibiotic ointments according to the 4th Yugoslav Pharmacopoeia is a hydrophobic base containing only aliphatic hydrocarbons. The fact that antibiotics are predominantly not lipophylic raises the question about the suitability of that particular type of the base for the manufacturing of antibiotic ointments. Recent studies of the lipid analysis of the skin corneal layer indicated that lipids had shown the bilamellar organization in the skin intercorneal space. Such structural organization could be seen in the ambiphylic bases whose structure was based on carefully selected emulsifier couple, consisting of the lyotropic and thermotropic liquid crystals. The aim of this study was to test the velocity of antibiotics (klindamycin hydrochloride, erythromycin base and chloramphenicol) release from the hydrophobic ointment-type bases, and from ambiphylic bases of anionic and non-ionic types. Methods. Membrane-free agar diffusion test as the basic method for testing the release velocity in vitro and Staphylococcus aureus as the strain highly susceptible to the chosen antibiotics were used. All the analyzed samples were manufactured as the suspension-type ointments. Results. The highest growth inhibition zone of the Staphylococcus aureus strain for all three analyzed antibiotics was achieved from the non-ionogenic ambiphylic base; the clear growth inhibition zone area for Staphylococcus aureus strain in the preparations containing anionic ambiphylic base was smaller by 10-31.28%, and in the preparations containing aliphatic hydrocarbon-type base, the decrease was 11.46-31.28%, compared to the results achieved with the non-ionogenic ambiphylic base. Conclusion. The optimal release velocity for the analyzed antibiotics was achieved from the non-ionic ambiphylic base.

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EFEKTIVITAS EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) DAN DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav) TERHADAP ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus

STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa ◽

10.36456/stigma.vol10.no1.a635 ◽

2017 ◽

Vol 10 (01) ◽

Author(s):

F.E Afiff ◽

Susie Amilah

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Leaf Extract ◽

Inhibition Zone ◽

Morinda Citrifolia ◽

Diffusion Method ◽

Betel Leaf ◽

Growth Inhibition Zone ◽

Large Growth ◽

Method Paper

Has conducted research on the effectiveness of various concentrations of noni leaf extract (Morinda citrifolia L.) And red betel leaf (Piper crocatum Ruiz & Pav) the growth inhibition zone of staphylococcus aureus. Inhibition test using paper disc diffusion method. Paper discs soaked in noni leaf extract and red betel leaf for 30 minutes and placed on media that has been spread bacteria staphylococcus aureus and then incubated for 24 hours. The results showed that noni leaf extract and red betel leaves with a concentration of 40%, 60% and 80% affecting large growth inhibition zone of Staphylococcus aureus.

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KEEFEKTIFAN EKSTRAK MENGKUDU PADA BERBAGAI KONSENTRASI TERHADAP PENGHAMBATAN PERTUMBUHAN BAKTERI RALSTONIA SP. SECARA IN VITRO

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.2483-88 ◽

2004 ◽

Vol 4 (2) ◽

pp. 83-88

Author(s):

Efri Efri ◽

Titik Nur Aeny

Keyword(s):

Growth Inhibition ◽

Growth Inhibitor ◽

Inhibition Zone ◽

Morinda Citrifolia ◽

In Vitro Growth ◽

Fruit Extract ◽

Bacterial Wilt Disease ◽

Growth Inhibition Zone ◽

Medicinal Properties

Effectivity of Morinda citrifolia extract on in-vitro growth inhibition of Ralstonia sp. Morinda citrifolia has been known as one of plants having numerous medicinal properties. Extract of the fruits shows antibacterial activity against several bacteria including Pseudomonas aeruginosa. The purpose of this study was to observe inhibitor effect of M. citrifolia fruit extract, and to investigate the effect of different concentrations of M. citrifolia fruit extract on in-vitro growth of Ralstonia (Pseudomonas) sp., the causal agent of banana bacterial wilt disease in Lampung. Inhibition effect of M. citrifolia was determined by the presence of inhibition zone, and concentration effect was determined by the diameter of inhibition zone . The study consisted of two experiments. The treatments of the first experiment were three different kinds of fruit extract, namely the whole fruit, the seeds, and the fleshy parts, and the second one consisted of six different levels of extract concentrations. The results showed that the whole fruit extract was the most effective as an in-vitro growth inhibitor, and the higher the concentration level the higher the growth inhibition zone formed.

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Effects of Different Solvents to Extract Phytochemical Materials of Rhazya stricta Against Salmonella typhimurium Activity

Gene Cell and Tissue ◽

10.5812/gct.117543 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Bahman Fazeli Nasab ◽

Hasan Ahmadi ◽

Mehrangiz Ghafari

Keyword(s):

Medicinal Plants ◽

Growth Inhibition ◽

Salmonella Typhimurium ◽

Ethanolic Extract ◽

Inhibition Zone ◽

Average Growth ◽

Inhibition Zone Diameter ◽

Zone Diameter ◽

Rhazya Stricta ◽

Growth Inhibition Zone

Background: The use of plant extracts prepared from medicinal plants is common in the developing countries. Meanwhile, due to the resistance of microorganisms to antibiotics, researchers are looking to discover new antimicrobials. Medicinal plants play an essential role in this regard. Objectives: This study was aimed at investigating the antimicrobial properties of various extracts of Rhazya stricta plant against Salmonella typhimurium. Methods: Methanolic, aqueous, ethanolic, hydroalcoholic, and ethyl acetate extracts of R. stricta were prepared. S. typhimurium strains were isolated from poultry feces. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of this plant. The diameter of the growth inhibition zone using was determined using Whatman paper. Results: The lowest MIC against S. typhimurium was obtained from the hydroalcoholic solvent with 3.1 ppm. The most effective extraction solvent to inhibit the growth of S. typhimurium was the hydroalcoholic type with an average growth inhibition zone diameter of 12.25 mm, followed by the ethanolic extract with a growth inhibition zone diameter of 12.12 mm. Conclusions: According to the results of research and increasing resistance to synthetic antibacterial substances, it seems that R. stricta plant with the help of hydroalcoholic solvent, can be considered an effective plant in eliminating some bacteria, including S. typhimurium.

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Infestation of tall fescue (Festuca arundinacea Schreb.) with Neotyphodium coenophialum and its influence on growth of chosen microorganisms in vitro

Acta Agrobotanica ◽

10.5586/aa.2005.063 ◽

2012 ◽

Vol 58 (2) ◽

pp. 369-380 ◽

Cited By ~ 4

Author(s):

Dariusz Pańka

Keyword(s):

Growth Inhibition ◽

Tall Fescue ◽

Festuca Arundinacea ◽

Inhibition Zone ◽

Bipolaris Sorokiniana ◽

Gaeumannomyces Graminis ◽

Neotyphodium Coenophialum ◽

Mycelium Growth ◽

Growth Inhibition Zone

Occurrence of Neotyphodium coenophialum in tall fescue cultivars cultivated in Poland and determination an endophyte inhibition effect on mycelium growth of chosen microorganisms in vitro were investigated. Seventeen seed lots of 11 cultivars of tall fescue were examined. The endophyte mycelium was dyed with bengal rose and microscopically examined to detect N. coenophialum. Occurrence of endophyte was checked with PCR method. Influence of endophyte on growth of 15 microorganisms was established in the laboratory conditions on Petri dishes with PDA medium at 10, 20 and 30°C. Neotyphodium coenophialum occurred only in two seed lots, 'Barrocco' - 42% and Terros - 2%. Living mycelium of endophyte was isolated only from 'Barrocco'. The highest mycelium growth inhibition of Bipolaris sorokiniana, Fusarium avenaceum, F. equiseti, Microdochium nivale and Gaeumannomyces graminis by endophyte at 30°C was recorded. The highest width of growth inhibition zone (4mm) was detected for the last pathogen. Mycelium growth of B. sorokiniana and M. nivale was not inhibited at 10°C, and for F. avenaceum at 10 and 20°C.

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