Physicochemical composition and antimicrobial potential of stingless honey: a food of differentiated quality

This study aimed to assess the antimicrobial activity of various honeys against strains of gram-negative and -positive bacteria, as well as to determine the physicochemical parameters of these honeys. Seven honeys from various species of stingless bees were evaluated. The physical-chemical parameters evaluated were pH, moisture, water activity, acidity, ash, electrical conductivity and color. Antimicrobial activity was determined using disc diffusion agar tests and minimum inhibitory concentrations. We found that there was a relationship between the physical-chemical parameters and the antimicrobial activity. The minimum inhibitory concentration of 25% honey was able to inhibit the growth of both gram-positive and -negative bacteria; the greatest efficacy was verified for the species of bees Melipona mondury, M. quadrifasciata, Scaptotrigona bipunctata and Tetragona clavipes. Regarding synergism, Escherichia coli maintained its sensitivity profile in relation to all studied honeys combined with antimicrobials. An important factor to consider is the concentration of honey capable of sensitizing the microorganism, as it has been shown to be dependent on the species of the stingless bee. Nevertheless, all honeys showed antimicrobial activity in various methods of analysis. These data suggest that honey is a promising alternative to sensitize resistant microorganisms, for the health of humans and animals alike.

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The antimicrobial activity of honeys produced in theCzech Republic

Czech Journal of Animal Science ◽

10.17221/4180-cjas ◽

2011 ◽

Vol 50 (No. 8) ◽

pp. 376-384 ◽

Cited By ~ 6

Author(s):

L. Vorlová ◽

R. Karpíšková ◽

I. Chabinioková ◽

K. Kalábová ◽

Z. Brázdová

Keyword(s):

Antimicrobial Activity ◽

Czech Republic ◽

Physicochemical Parameters ◽

Inhibitory Concentration ◽

Chemical Parameters ◽

Water Conductivity ◽

The Czech Republic ◽

Concentration Method ◽

Physical And Chemical

The aim of this project was to determine the antimicrobial activity of honeys produced in the Czech Republic to some bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli). Another aim was to find out whether there are correlations between the antimicrobial activity of honeys and their physicochemical parameters. All honeys included in the study were directly obtained from beekeepers in Southern and Northern Moravia from the summer load of 2001. The project contains 20 honeys which were determined according to their conductivity as blossom (6), blends (10) and honeydew (4). The determination of physical and chemical parameters such as content of water, conductivity, pH, water activity, invertase and diastase activities was carried out in accordance with the methods described in Harmonised methods of the European Honey Commission. The determination of the antimicrobial activity of honey was done by the MIC (minimal inhibitory concentration) method. Statistically significant relations between the antimicrobial activity and the conductivity of honey (P < 0.05), the diastase (P < 0.05) and invertase (P < 0.001) activities were found out in the observed physicochemical parameters. The study proved that honey produced in the Czech Republic is antimicrobially effective with the highest effect in honeydew honeys (P < 0.01).

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Physical-Chemical Parameters of Honey of Stingless Bee (Hymenoptera: Apidae)

American Chemical Science Journal ◽

10.9734/acsj/2015/17547 ◽

2015 ◽

Vol 7 (3) ◽

pp. 139-149 ◽

Cited By ~ 10

Author(s):

Andreia Nascimento ◽

Luis Marchini ◽

Carlos Carvalho ◽

Diogo Araújo ◽

Ricardo Olinda ◽

...

Keyword(s):

Stingless Bee ◽

Chemical Parameters ◽

Physical Chemical

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Can rhamnose-based glycolipids nanoparticles be an alternative to fight biofilms on medical devices?

10.5194/biofilms9-94 ◽

2020 ◽

Author(s):

Carolina Tomé ◽

Inês Anjos ◽

Victor Martin ◽

Catarina Santos ◽

Lidia Gonçalves ◽

...

Keyword(s):

Antimicrobial Activity ◽

Zeta Potential ◽

Medical Devices ◽

Encapsulation Efficiency ◽

Inhibitory Concentration ◽

Control Cell ◽

Chitosan Nanoparticles ◽

Biofilm Inhibition ◽

Promising Alternative ◽

Biofilm Development

Biofilm development on medical devices is of particular concern and finding new strategies for preventing surface colonization and infection development are urgent. Antimicrobial biosurfactants such as rhamnolipids (RLs), emerge as one possible solution due their lack of resistance development. Using nanoparticles as delivery systems for these compounds may be a promising alternative in the context of biofilm-infections control. As such, the aim of this study was to encapsulate RLs into chitosan nanoparticles (RLs-NPs), test their antimicrobial activity and their biocompatibility profile. Blank nanoparticles (b-NPs) and RLs-NPs were prepared by ionic gelation. For particles characterization, zeta potential, size distribution and encapsulation efficiency were performed. Minimal inhibitory concentration and biofilm inhibition ability were evaluated towards Staphylococcus aureus (ATCC 25923). To access NPs cytocompatibility the in vitro tetrazolium dye assay (MTT) and morphology observation were performed with a mouse fibroblastic cell line (L929). RLs-NPs presented an encapsulation efficiency of 74.2&#177;1.3%, a size ranging from 300 to 400 nm and a zeta potential of&#160; 37&#177;1 mV. The minimum inhibitory concentration of RLs-NPs was 130 mg/mL and a 99% biofilm inhibition was achieved with these NPs meaning that their antimicrobial activity is also effective towards sessile bacteria. When compared to control, cell cultures grown in the presence of RLs-NPs presented no significant differences regarding the MTT reduction values and morphology analysis, suggesting that NPs up to 500&#160;mg/mL did not significantly interfere with viability and proliferation. The results revealed that the RLs-NPs were able to inhibit bacterial growth showing adequate cytocompatibility and might become, after additional studies, a possible approach to fight S. aureus biofilm associated infections. Acknowledgments: Support for this work was provided by FCT through Portuguese government, PTDC/BTM-SAL/29335/2017 and Pest-UID/DTP/04138/2019

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Screening of the antimicrobial activity of marine sponge extracts from Martinique

10.1101/2021.02.16.431398 ◽

2021 ◽

Author(s):

Julie Piron ◽

Jessica Pastour ◽

Niklas Tysklind ◽

Juliette Smith-Ravin ◽

Fabienne Priam

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Marine Sponges ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Staphylococcus Saprophyticus

AbstractMarine sponges are known for their antimicrobial, antifungal, and cytotoxic activity. In this study, the activity of aqueous and ethanoic extracts of 3 sponges from Martinique were tested on 5 bacterial strains: Bascillus cereus (CIP 783), Echerichia coli (CIP 54127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125). The antimicrobial activity of Agelas clathrodes, Desmapsamma anchorata, and Verongula rigida, was demonstrated using the disc diffusion method and by determining the minimum inhibitory concentration and the minimum bactericidal concentration. The ethanoic extract of Agelas clathrodes had an inhibitory activity specifically on Staphylococcus aureus and Staphylococcus saprophyticus. No activity was observed for the other extracts. Further chemical analyses will be carried out in order to identify the active molecules of these sponges.

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Antimicrobial Properties of 6-Bromoeugenol and Eugenol

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.53.57 ◽

2016 ◽

Vol 53 ◽

pp. 57-64

Author(s):

Radia Mahboub ◽

Faiza Memmou

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

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ANTIMICROBIAL ACTIVITY OF MIXTURE FROM DIFFERENT PLANT EXTRACTS IN GEL, CREAM AND BODY MILK FORMULATION, AGAINST DIFFERENT MICROORGANISMS

Contributions Section of Natural Mathematical and Biotechnical Sciences ◽

10.20903/csnmbs.masa.2014.35.2.59 ◽

2017 ◽

Vol 35 (2) ◽

Author(s):

Dzoko Kungulovski ◽

Natalija Atanasova-Pancevska

Keyword(s):

Antimicrobial Activity ◽

Minimal Inhibitory Concentration ◽

Plant Extracts ◽

Broad Spectrum ◽

Inhibitory Concentration ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Inhibition Zones ◽

Standard Disc

In this study the antimicrobial activity of a mixture of plant extracts originating from five different plants and suspended in three different forms of gel, cream and lotion was examined with the purpose of discovering new anti-microbial compounds. The antimicrobial activity was investigated through the standard disc diffusion method, as well as through a variation of the microdillution method. The formulations under examination (gel, cream and lotion) showed a broad spectrum of action against all the selected microorganisms, with inhibition zones of 9–46 mm. The minimal inhibitory concentration (MIC) for all the formulations against the microorganisms in the study was in the range of 0.000761μg ml-1 to 0.125μg ml-1. The results of this study have clearly demonstrated that the mixture of plant extracts originating from five different plants and suspended in the forms of gel, cream and lotion can definitely be used in the battle against the microorganisms under investigation.

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ANTIMICROBIAL ACTIVITY OF 3- (1- (3- HYDROXYPHENYL) AMINO) ETHYLIDENE) CHROMAN-2,4-DIONE AND ITS CORRESPONDING PALLADIUM(II) COMPLEX

10.46793/iccbi21.387a ◽

2021 ◽

Author(s):

Edina Avdović ◽

◽

Žiko B. Milanović ◽

Milanka Radulović ◽

Dušan S. Dimić

Keyword(s):

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Antimicrobial Effect ◽

Coumarin Derivative ◽

The Other ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Microdilution Method ◽

Bacteria And Fungi

In this manuscript, the in vitro antimicrobial activity of the previously synthesized coumarin derivative 3- (1- (3-hydroxyphenyl) amino) ethylidene) chroman-2,4-dione (L) and its corresponding palladium (II) complex (C) were examined. Their antimicrobial activity was screened against four strains of bacteria Bacillus cereus (ATCC 11778) G+; Staphylococcus aureus (ATCC 13709) G+; Klebsiella pneumoniae (ATCC 27736) G-; and Escherichia coli (ATCC 2592) G-) and three strains of fungi (Aspergillus flavus (ATCC15517); Candida albicans (ATCC 10231); Fusarium oxysporum (ATCC 695) using disc diffusion and microdilution method. The obtained minimum inhibitory concentration (MIC) values by microdilution method for ligand and complex are similar for all tested bacteria and fungi, which means that both compounds have a similar antimicrobial effect. On the other hand, analysis of zone of inhibition (ZI) values for the tested compounds shows that the complex is generally somewhat more active than the ligand.

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In vitro antimicrobial activity of total extracts of the leaves of Petiveria alliacea L. (Anamu)

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502013000200006 ◽

2013 ◽

Vol 49 (2) ◽

pp. 241-250 ◽

Cited By ~ 2

Author(s):

Ania Ochoa Pacheco ◽

Jorge Marín Morán ◽

Zenia González Giro ◽

Adrian Hidalgo Rodríguez ◽

Rachel Juliet Mujawimana ◽

...

Keyword(s):

Antimicrobial Activity ◽

Isopropyl Alcohol ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Antimicrobial Effect ◽

Bacterial Strains ◽

Chemical Parameters ◽

Petiveria Alliacea ◽

Quality Control Parameters

The antimicrobial activity of 13 total extracts was evaluated, 10 soft extracts (B) and 3 blended extracts (E) prepared from dry and fresh leaves of Petiveria alliacea L. Various solvents were used for their preparation: hydroalcoholic solution at 30%, 80% and isopropyl alcohol. The antimicrobial effect of the extracts was tested by means of the method of Kirby-Bauer, using four bacterial strains from the ATCC collection (Escherichia coli, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa) and a leveduriform fungus (Candida albicans). The following quality control parameters were determined for most active extracts: physical, physical-chemical and chemical parameters. The results were: nine extracts showed antibacterial activity, being the most concentrated (B8 and E3), the ones with the highest activity in the presence of the bacteria tested; the effect of blended extracts (E1, E2 and E3) was greater in the presence of P. aeruginosa. Blended extracts are considered more potent and active than soft extracts. No antifungal activity was obtained for both types of extracts. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined for both extracts, with the following results: MIC-soft extracts (>100 mg/mL), blended extracts (>50 mg/mL); MBC-soft extracts (≥400 mg/mL), blended extracts (≥200 mg/mL) based on fresh leaves.

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Comparative Analysis of In vitro Antimicrobial and Antioxidant Potential of Cinnamomum tamala Extract and their Essential Oils of Two Different Chemotypes

Agricultural Science Digest - A Research Journal ◽

10.18805/ag.d-5187 ◽

2021 ◽

Author(s):

Gaurav Naik ◽

Syed Zafar Haider ◽

Ujjwal Bhandari ◽

Hema Lohani ◽

Nirpendra Chauhan

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oils ◽

Inhibitory Concentration ◽

Gas Chromatography Mass Spectrometry ◽

Disc Diffusion ◽

Disc Diffusion Assay ◽

Diffusion Assay

Background: The essential oils of aromatic plants have wide range of biological applications. Natural food preservatives have been always a demanding for food industries in both developed and developing countries to prevent bacterial growth in food stuffs. Therefore, focused on Cinnamon leaves essential oils components against food pathogens have been investigated to confirm its potential use in food products. Methods: The antimicrobial activity of two Cinnamon leaves oils and extracts (T-2 and T-19) were examined by disc diffusion assay and the minimum inhibitory concentration by two-fold serial dilution method against foodborn pathogenic microorganisms i.e. E.coli (MTCC 723), B. Cereus (MTCC 430), S. aureus (MTCC 3381), S. typhi (MTCC 734) and C. perfringens (MTCC 1349). The antioxidant activity of both essential oils and extract was determined by DPPH assay. The chemical profiling of Cinnamon essential oils were determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Result: The antimicrobial activity of both Cinnamon leaves oils and extract were evaluated by disc diffusion assay and it showed that in essential oils and extracts exhibited the highest zone of inhibition (ZOI) against S. aureus and E.coli. Minimum inhibitory concentration (MIC) of both oils and extracts ranged from 0.156 mg/ml to 5mg/ml and the antioxidant properties of oils and extract of cinnamaldehyde type Cinnamon possessed the highest antioxidant activity than linalool type. The chemical constituent of Cinnamon oil was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) revealed that T-2 contains cinnamaldehyde (75%) and T-19 contains Linalool (63.77%) were found as major constituents. Therefore the results shows that essential oil of cinnamaldehyde type Cinnamon could be a potential rich source of natural antioxidants and also more effective against food borne pathogens than linalool type and could be used as natural antibacterial agents in food preservation.

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Phytochemical screening and antimicrobial activity of extracts of Cassia alata L. leaves and seeds

Bulgarian Chemical Communications ◽

10.34049/bcc.51.3.5049 ◽

2019 ◽

Vol 51 (3) ◽

pp. 378-383

Author(s):

P. T. Q. Le

Keyword(s):

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Diffusion Method ◽

Disc Diffusion ◽

Phytochemical Screening ◽

Disc Diffusion Method ◽

Total Polyphenol ◽

E Coli ◽

Cassia Alata ◽

Paper Disc

The aim of the study is to determine the presence of some bioactive compounds in Cassia alata L. leaves and seeds extracts such as tannins, saponins, anthraquinones and flavonoids. Total polyphenol contents (TPC) of leaves and seeds extracts are 59.211 mg GAE/g DW and 1.816 mg GAE/g DW, respectively, while their antioxidant capacities (AC) are 8.14 μmol Fe/g DW and 2.75 μmol Fe/g DW, respectively. The antimicrobial activity is determined by the paper disc diffusion method combined with the minimum inhibitory concentration (MIC). Leaves extract inhibits S. aureus and E. coli at MIC of 400 mg/mL; S. enteritidis and B. subtilis at MIC of 800 mg/mL. Besides, seeds extract also inhibits S. aureus at MIC of 200 mg/mL; E. coli, S. enteritidis and B. subtilis at MIC of 400 mg/mL. However, leaves and seeds extracts of C. alata do not show any inhibitions on the growth of A. niger. Keywords: Antibacterial activity, Antioxidant, Cassia alata L., Extract, MIC.

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