scholarly journals The antimicrobial activity of honeys produced in theCzech Republic

2011 ◽  
Vol 50 (No. 8) ◽  
pp. 376-384 ◽  
Author(s):  
L. Vorlová ◽  
R. Karpíšková ◽  
I. Chabinioková ◽  
K. Kalábová ◽  
Z. Brázdová
Keyword(s):  
Antimicrobial Activity ◽  
Czech Republic ◽  
Physicochemical Parameters ◽  
Inhibitory Concentration ◽  
Chemical Parameters ◽  
Water Conductivity ◽  
The Czech Republic ◽  
Concentration Method ◽  
Physical And Chemical

 The aim of this project was to determine the antimicrobial activity of honeys produced in the Czech Republic to some bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli). Another aim was to find out whether there are correlations between the antimicrobial activity of honeys and their physicochemical parameters. All honeys included in the study were directly obtained from beekeepers in Southern and Northern Moravia from the summer load of 2001. The project contains 20 honeys which were determined according to their conductivity as blossom (6), blends (10) and honeydew (4). The determination of physical and chemical parameters such as content of water, conductivity, pH, water activity, invertase and diastase activities was carried out in accordance with the methods described in Harmonised methods of the European Honey Commission. The determination of the antimicrobial activity of honey was done by the MIC (minimal inhibitory concentration) method. Statistically significant relations between the antimicrobial activity and the conductivity of honey (P < 0.05), the diastase (P < 0.05) and invertase (P < 0.001) activities were found out in the observed physicochemical parameters. The study proved that honey produced in the Czech Republic is antimicrobially effective with the highest effect in honeydew honeys (P < 0.01).  

scholarly journals Safety and quality of farm fresh goat’s cheese in the Czech Republic

2010 ◽  
Vol 28 (No. 1) ◽  
pp. 1-8 ◽  
Author(s):  
B. Janštová ◽  
M. Dračková ◽  
Š. Cupáková ◽  
H. Přidalová ◽  
M. Pospíšilová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Dry Matter ◽  
Titratable Acidity ◽  
Chemical Characteristics ◽  
Chemical Parameters ◽  
Salmonella Spp ◽  
The Czech Republic ◽  
Enterococcus Spp ◽  
Physical And Chemical

The composition and selected physical and chemical parameters of 44 samples of fresh goat cheeses produced on a farm in the Czech Republic were determined. The following average values were obtained for the parameters analysed: pH 4.87 &plusmn; 0.14, titratable acidity (SH) 98.09 &plusmn; 4.93, dry matter 46.83 &plusmn; 1.57%, fat in dry matter 52.74 &plusmn; 5.24%, sodium chloride (NaCl) 2.08 &plusmn; 0.54%, and a<sub>w</sub> 0.979 &plusmn; 0.007. All samples showed excellent sensory characteristics and their compositions corresponded to those declared by the producer. Microbiological tests were used for the detection of Enterobacteriaceae spp., lactic acid bacteria, Escherichia coli, Enterococcus spp., Staphylococcus aureus, Bacillus cereus, Salmonella spp. and Listeria monocytogenes. Under the applicable regulations, the analysed fresh goat&rsquo;s cheeses were microbiologically safe and had the appropriate physical and chemical characteristics.

scholarly journals Physicochemical composition and antimicrobial potential of stingless honey: a food of differentiated quality

2020 ◽  
Vol 9 (10) ◽  
pp. e7099108223
Author(s):  
Thaisa Francielle Toposlki Pavan Batiston ◽  
Angelica Frigo ◽  
Lenita Moura Stefani ◽  
Aleksandro Schafer Da Silva ◽  
Denise Nunes Araujo
Keyword(s):  
Antimicrobial Activity ◽  
Physicochemical Parameters ◽  
Inhibitory Concentration ◽  
Stingless Bee ◽  
Disc Diffusion ◽  
Chemical Parameters ◽  
Promising Alternative ◽  
Physicochemical Composition ◽  
Physical Chemical ◽  
Sensitivity Profile

This study aimed to assess the antimicrobial activity of various honeys against strains of gram-negative and -positive bacteria, as well as to determine the physicochemical parameters of these honeys. Seven honeys from various species of stingless bees were evaluated. The physical-chemical parameters evaluated were pH, moisture, water activity, acidity, ash, electrical conductivity and color. Antimicrobial activity was determined using disc diffusion agar tests and minimum inhibitory concentrations. We found that there was a relationship between the physical-chemical parameters and the antimicrobial activity. The minimum inhibitory concentration of 25% honey was able to inhibit the growth of both gram-positive and -negative bacteria; the greatest efficacy was verified for the species of bees Melipona mondury, M. quadrifasciata, Scaptotrigona bipunctata and Tetragona clavipes. Regarding synergism, Escherichia coli maintained its sensitivity profile in relation to all studied honeys combined with antimicrobials. An important factor to consider is the concentration of honey capable of sensitizing the microorganism, as it has been shown to be dependent on the species of the stingless bee. Nevertheless, all honeys showed antimicrobial activity in various methods of analysis. These data suggest that honey is a promising alternative to sensitize resistant microorganisms, for the health of humans and animals alike.

A Comparative Analysis of the Power of Selected European Countries. The Economic Determinants

2021 ◽  
Vol 37 (37) ◽  
pp. 22-42
Author(s):  
Alicja Paluch ◽  
Henryk Spustek
Keyword(s):  
Czech Republic ◽  
Comparative Analysis ◽  
European Countries ◽  
Economic Power ◽  
Strongly Correlated ◽  
The Czech Republic ◽  
Independent Variables ◽  
Economic Sphere ◽  
National Power

The ever-increasing need for in-depth analysis and quantification of the national power, in particular ‘hard’ and ‘soft’ power-generating factors as well as difficulties in identifying a comprehensive and effective method for scientific determination of the national power, have given rise to research in the indicated scientific issues within this article. The presented considerations aim to define the assumptions for a descriptive sub-model that would enable a comparison of Poland’s power in the economic sphere (which is a component of the non-military sphere) with the power of selected European countries. The research hypothesis is that, among the variety of descriptive variables in the economic sphere of the national power, there is a subset of mutually independent variables, at the same time strongly correlated with the national power, which make it possible to define assumptions for the sub-model of the national power. The steps of the research procedure were carried out using the method of system analysis (multi-criteria comparative analysis) and statistical analysis. The research activities undertaken have shown that the factors that are strongly correlated with the national power in the economic area of the European countries adopted for the analysis are: dynamics of industrial production, private sector credit flows and economic freedom index. The comparative analysis carried out demonstrates that the greatest increase in the economic power in the analysed period took place in Germany (0.68). Slightly smaller growth was recorded in the Czech Republic (0.62) and Poland (0.60), while the lowest value of increase was in Romania (0.23). The conducted qualitative comparative analysis of the economic power of selected European countries allowed to conclude that the independent variables identified are crucial for the formation of the economic power of the analysed countries. At the same time, a fairly strong position of the Czech Republic and Poland in relation to the economic power of Germany was found. The performed quantification of the economic power of the European countries provides a basis for the correct determination of changes in the power distribution of political units, assessment of the power and resources held by the state.

scholarly journals Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

2020 ◽  
Vol 17 (15) ◽  
pp. 5508 ◽  
Author(s):  
Hana Mlejnkova ◽  
Katerina Sovova ◽  
Petra Vasickova ◽  
Vera Ocenaskova ◽  
Lucie Jasikova ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Wastewater Treatment ◽  
Czech Republic ◽  
Wastewater Treatment Plants ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Untreated Wastewater ◽  
Preliminary Study ◽  
Polymerase Chain

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.

scholarly journals Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

2008 ◽  
Vol 25 (No. 4) ◽  
pp. 214-220 ◽  
Author(s):  
J. Simonová ◽  
M. Vázlerová ◽  
I. Steinhauserová
Keyword(s):  
Czech Republic ◽  
Yersinia Enterocolitica ◽  
Virulence Genes ◽  
Optimum Time ◽  
The Czech Republic ◽  
Specific Identification ◽  
Time Consumption ◽  
Serotype O

In this study, the pathogenic <i>Y. enterocolitica</i> of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as well as PCR methods based on the identification of virulence genes (<i>ail</i>, <i>rfbC</i>, <i>ystA</i>, <i>yadA</i>, <i>virF</i>). The occurrence of <i>Y. enterocolitica</i> O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two <i>Y. enterocolitica</i> O:3 isolates were identified by biochemical and serologic methods, and 53 <i>Y. enterocolitica</i> O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of <i>Y. enterocolitica</i> O:3 carried genes <i>ail</i> and <i>rfbC</i>, 83% isolates carried gene <i>ystA</i>, 79% isolates carried gene <i>yadA</i> and 49% isolates carried gene <i>virF</i>. The use of PCR methods based on the identification of <i>ail</i> and <i>rfbC</i> genes provides for a sufficiently specific identification of pathogenic <i>Y. enterocolitica</i> O:3 strains with optimum time consumption compared to biochemical and serological methods. It is not recommendable to use other PCR methods (detection of the <i>ystA, <i>yadA</i>, and <i>virF</i> genes) for the detection of pathogenic <i>Y. enterocolitica</i> strains because those methods are not very specific for the determination of pathogenicity.

scholarly journals Limiting factors in the field of business activities in rural tourism

2008 ◽  
Vol 53 (No. 9) ◽  
pp. 421-431 ◽  
Author(s):  
K. Ryglová
Keyword(s):  
Czech Republic ◽  
Rural Areas ◽  
Rural Population ◽  
Rural Tourism ◽  
The Other ◽  
Limiting Factors ◽  
The Czech Republic ◽  
Tourist Infrastructure ◽  
The Individual

This paper deals with problems of rural tourism development. The works is focused on researching the situation in the area of rural tourism in the Czech Republic, on understanding the attitudes of entrepreneurial sphere and rural population to this entrepreneurship and mainly on the determination of limiting factors that prevent this form of tourism from a more distinctive development. Rural tourism in the Czech Republic is still in the initiating stage of its development and it is not as developed as in some countries in West Europe. This goal has been reached with the help of primary questionnaire inquiry among business and agricultural subjects in rural areas in the individual regions and the detail results of this questionnaire inquiry are enclosed to the following paper. These identified factors were mainly insufficient financial means and at the same time insufficient state assistance connected with this. Insufficient awareness of this support as well as its inaccessibility for small business appears to be a difficulty, too. From the other obstacles, we can mention the current legislation system, bureaucracy, the state of communication and tourist infrastructure, fears of neighbours’ intolerance and losing privacy and also unfamiliarity and little experience.

Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC

2008 ◽  
Vol 56 (11) ◽  
pp. 4092-4095 ◽  
Author(s):  
Lenka Jedličková ◽  
David Gadas ◽  
Pavla Havlová ◽  
Josef Havel
Keyword(s):  
Czech Republic ◽  
The Czech Republic

scholarly journals The accuracy of FOM instrument used in on-line pig carcass classification in the Czech Republic

2008 ◽  
Vol 52 (No. 6) ◽  
pp. 149-158 ◽  
Author(s):  
M. Šprysl ◽  
J. Čítek ◽  
R. Stupka ◽  
L. Vališ ◽  
M. Vítek
Keyword(s):  
Czech Republic ◽  
Measurement Errors ◽  
Muscle Thickness ◽  
The Czech Republic ◽  
High Agreement ◽  
Lean Meat ◽  
Depth Measurement ◽  
Operator Error ◽  
On Line

The objective of this study was to document the accuracy of the classification equipment used in the Czech Republic with respect to measurement errors in lean meat percentage prediction such as point of measurement error, equipment error as well as operator error. To this end, a total of 720 pigs were measured in one abattoir. One can say from the results that the correlations between correct and surrogate measurements of fat depth are high (0.95&minus;0.98), for muscle thickness they are lower ranging from 0.49 to 0.88, and for lean meat percentage they are in the range of 0.85&minus;0.95. The lowest correlation (0.49) was calculated for muscle depth measurement between the 2nd and 3rd last rib when the place of measurement was moved 1 cm in the caudal direction, which influenced the level of the FOM correlation. It was further demonstrated that for the second insertion the differences in lean meat percentage prediction ranged from &minus;6.07% to +9.29%. It was also demonstrated that various instruments provided identical measurements of fat depth (<i>r</i> = 0.57&minus;0.97), while for muscle depth the performance was worse (<i>r</i> = 0.38–0.78), which caused a fluctuation in the prediction of lean meat percentage with differences ranging from &minus;2.56% to +2.81%. It can also be concluded that a high agreement between operators was demonstrated for the determination of lean meat percentage (<i>r</i> = 0.71&minus;0.80).

scholarly journals Evaluation of Antimicrobial Activity and Synergistic Effect of Spices against Few Selected Pathogens

2019 ◽  
Vol 6 ◽  
pp. 10-18
Author(s):  
Renuka Maharjan ◽  
Saru Thapa ◽  
Amrit Acharya
Keyword(s):  
Antimicrobial Activity ◽  
Synergistic Effect ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Ethanolic Extract ◽  
Fractional Inhibitory Concentration ◽  
Zone Of Inhibition ◽  
Soxhlet Apparatus ◽  
Mic Value

Objectives: The main objective of this study was to evaluate antimicrobial activity of ethanolic extract of spices along with determination of its synergistic effect against few selected pathogens. Methods: In this study, ethanolic extract of 5 different spices; Zingiber officinale (Ginger), Allium sativum (Garlic), Curcuma longa (Turmeric), Capsicum annum (Chili) and Allium cepa (Onion) were obtained by using Soxhlet apparatus. The ethanolic extract was concentrated by evaporation and different concentrations of extract were prepared in Dimethy Sulphoxide (DMSO) solvent. Test organisms included mainly pathogens i.e. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae. The antimicrobial activities of the extracts were determined by well diffusion technique both individually and in combination. On the other hand, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was determined by serial dilution technique. The result were interpreted on the basis of the fact that the growth occurs in positive control and other tubes with inadequate amount of extract whereas the lowest concentration of agent that inhibits growth of organism, detected by lack of visible turbidity by inhibition of 99% is designed as the MIC. The MBC is identified by determining the lowest concentration of extract solution that reduces the viability of the initial bacterial inoculum by a predetermined reduction such as ≥99.9%. Likewise, for determination of Fractional Inhibitory Concentration Index (FICI), two extracts were combined along with standardized inoculum of bacterial strain. Tubes without visible turbidity were streaked on agar plate and observed for 99.9% killing.   Results: All the tested extract of spices were found effective against S. aureus and K. pneumoniae only. The highest zone of inhibition (ZOI) was found in chili extract (ZOI=26 mm) against S. aureus whereas lowest zone of inhibition was found in garlic extract against K. pneumoniae (ZOI=12mm). Similarly, highest ZOI was produced by combined extract of both Turmeric and Ginger (ZOI= 26 mm). Turmeric extract was found to be effective against S. aureus (MIC value = 62.5 mg /ml and MBC value = 31.25 mg/ml) and K. pneumoniae (MIC value 125 mg/ml and MBC value = 62.5 mg/ml). The Fractional Inhibitory Concentration (FIC) values of combined extract suggested synergistic and additive effect (0.5<FIC<1). Chili and ginger were effective with FIC value of 0.25. Conclusion: To recapitulate, the extract of spices can be used to prevent the pathogenic organism.

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