scholarly journals Markers of acute stress in pigs

2007 ◽  
pp. 323-329
Author(s):  
K Breineková ◽  
M Svoboda ◽  
M Smutná ◽  
L Vorlová
Keyword(s):  
Acute Stress ◽  
Endocrine System ◽  
Control Group ◽  
Blood Samples ◽  
Stress Markers ◽  
Transport Control ◽  
Porcine Stress Syndrome ◽  
Experimental Group ◽  
Pig Serum ◽  
Stimulation Of

This study explores the biological validation of markers of acute stress in pigs subjected to transportation for slaughter. The stress markers selected for monitoring were neopterin and cortisol. Their levels in pig serum were measured for two porcine stress syndrome genotypes, NN and Nn, after a 30-min transport to a slaughterhouse. Blood samples were withdrawn before transport (control group) and immediately after the animals' arrival (experimental group). The values of neopterin and cortisol measured before the transport were 5.60+/-1.65 nmol/l and 273.54+/-66.17 nmol/l respectively. After the transfer, the concentration of cortisol rose significantly compared to the control (355.69+/-85.13 nmol/l, p<0.01). Neopterin concentrations in the serum (8.25+/-1.60 nmol/l) were also significantly higher (p<0.01) after transportation. The elevated concentrations of both analytes were found to be independent of the genotype. These results document the stimulation of the endocrine system and the immune system that develops in animals undergoing transportation for slaughter.

scholarly journals Influence of reproductive status on the daily rhythms of oxidative stress markers in Ovis aries

2010 ◽  
Vol 5 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Giuseppe Piccione ◽  
Claudia Giannetto ◽  
Francesco Fazio ◽  
Anna Assenza ◽  
Giovanni Caola
Keyword(s):  
Oxidative Stress ◽  
Circadian Rhythms ◽  
Ovis Aries ◽  
Reproductive Status ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Daily Rhythms ◽  
Blood Samples ◽  
Stress Markers ◽  
Experimental Group

AbstractChanges in circadian rhythms of dROMs, Oxy-ads and SHp during reproductive stages were studied in Comisana ewes. Twelve ewes were divided in two equal groups. The experimental group consisted of ewes undergoing gestation and lactation following artificial insemination and the control group consisted of non-pregnant ewes. Blood samples were collected every 3 h over a 24 h period, 20 days before insemination, on days 100 and 140 of pregnancy, on days 10, 30 and 200 post-partum and during the dry period. In the control group, blood samples were collected on the same days and with the same procedures as those used for the experimental group. A significant effect of time on all parameters studied was observed in the experimental group. Daily rhythms of the parameters studied were observed in the control group in all experimental conditions, and in the experimental group during pre-pregnancy and dry periods. We conclude that the reproductive status of sheep affects oxidative stress markers in blood and their circadian rhythms.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

scholarly journals Salmonella Bacterin Vaccination Decreases Shedding and Colonization of Salmonella Typhimurium in Pigs

2021 ◽  
Vol 9 (6) ◽  
pp. 1163
Author(s):  
Eduarda Alexandra Gonçalves de Oliveira Moura ◽  
Daniela Gomes da Silva ◽  
Caio Henrique Turco ◽  
Thainara Vitoria Carnevalli Sanches ◽  
Gabriel Yuri Storino ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Blood Count ◽  
Control Strategies ◽  
Control Group ◽  
Blood Samples ◽  
Commercial Vaccine ◽  
Serum Igg ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Since the occurrence of swine salmonellosis has increased over time and control strategies other than biosecurity are highly recommended, the present study aimed to evaluate the efficacy of vaccination with Salmonella Choleraesuis and Salmonella Typhimurium bacterins in pigs. Two experimental groups were formed: G1, animals immunized with two doses of a commercial vaccine (n = 20); G2, control group (n = 20). After vaccination, all pigs were orally challenged (D0) with 108 CFU of Salmonella Typhimurium and evaluated for 40 days. Every 10 days after D0, five piglets from each experimental group were euthanized and submitted to the necroscopic examination, when organ samples were collected. Blood samples and rectal swabs were collected before the first dose of the vaccine (D−42), before the second dose (D−21), before the challenge (D0), and thereafter, every three days until D39. Blood count, serum IgG measurement by ELISA, and the excretion of Salmonella Typhimurium in feces were evaluated. While the results from blood count and serum IgG concentration did not differ, the detection and excretion of Salmonella between G1 and G2 differed (p < 0.05). Therefore, it was observed that this vaccine partially protected the animals against experimental infection with Salmonella Typhimurium, reducing the excretion of bacteria in feces.

Adhesion of Malassezia pachydermatis of different growth type to canine epithelial cells

2014 ◽  
Vol 17 (2) ◽  
pp. 365-366 ◽  
Author(s):  
M. Florek ◽  
J. Król ◽  
Z. Staroniewicz ◽  
B. Bażanów
Keyword(s):  
Vitamin E ◽  
Epithelial Cells ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Control Group ◽  
Growth Type ◽  
Malassezia Pachydermatis ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

scholarly journals Changes in the Concentrations of Some Plasma Proteins During Acute Inflammation in Dogs

2017 ◽  
Vol 1 (1) ◽  
pp. 85-89
Author(s):  
Dimirtinka Zapryanova ◽  
Teodora Mircheva ◽  
Tsanko Hristov ◽  
Lazarin Lazarov ◽  
Aleksander Atanasov ◽  
...  
Keyword(s):  
Body Weight ◽  
Plasma Proteins ◽  
Acute Inflammation ◽  
Control Group ◽  
Post Inoculation ◽  
Lumbar Region ◽  
Total Proteins ◽  
Blood Samples ◽  
Inflammatory Conditions ◽  
Experimental Group

Abstract The purpose of the present study was to analyse the changes in concentrations of total proteins, albumin, globulins and albumin/globulin ratio in dogs with experimentally induced acute inflammation. The study was performed on 9 mongrel dogs (experimental group) and 6 mongrel dogs (control group) at the age of 2 years and body weight 12-15 kg. The acute inflammation was reproduced by inoculation of 2 ml turpentine oil in the lumbar region subcutaneously and in same quantity saline in control dogs. Blood samples were collected into heparinized tubes before inoculation (hour 0) then at hours 6, 24, 48, 72 and on days 7, 14, 21. The statistical analysis of the data was performed using one way analysis of variance (ANOVA). The level of albumin statistically decreased in the experimental dogs from at 72nd h to day 14 while the concentration of globulins increased from the 72nd h to day 21. On days 7 and 14 the albumin/globulin ratio slightly decreased. During the whole post inoculation period the values of total protein have not changed. The dates of the present study confirm that albumin, albumin/globulin ratio and globulins are sensitive factors in inflammatory conditions in dogs.

scholarly journals Effect of Hemin on Brain Alterations and Neuroglobin Expression in Water Immersion Restraint Stressed Rats

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Merhan Ragy ◽  
Fatma Ali ◽  
Maggie M. Ramzy
Keyword(s):  
Acute Stress ◽  
Water Immersion ◽  
Stress Hormones ◽  
Control Group ◽  
Albino Rats ◽  
Stress Markers ◽  
Stressed Group ◽  
Total Antioxidant ◽  
The Brain ◽  
Blood Brain Barrier Damage

In the brain, the heme oxygenase (HO) system has been reported to be very active and its modulation seems to play a crucial role in the pathophysiology of neurodegenerative disorders. Hemin as HO-1 inducer has been shown to attenuate neuronal injury so the goal of this study was to assess the effect of hemin therapy on the acute stress and how it would modulate neurological outcome. Thirty male albino rats were divided into three groups: control group and stressed group with six-hour water immersion restraint stress (WIRS) and stressed group, treated with hemin, in which each rat received a single intraperitoneal injection of hemin at a dose level of 50 mg/kg body weight at 12 hours before exposure to WIRS. Stress hormones, oxidative stress markers, malondialdehyde (MDA), and total antioxidant capacity (TAC) were measured and expressions of neuroglobin and S100B mRNA in brain tissue were assayed. Our results revealed that hemin significantly affects brain alterations induced by acute stress and this may be through increased expression of neuroglobin and through antioxidant effect. Hemin decreased blood-brain barrier damage as it significantly decreased the expression of S100B. These results suggest that hemin may be an effective therapy for being neuroprotective against acute stress.

Keeping method influence on newborn calves organism

2020 ◽  
pp. 23-24
Author(s):  
Vera N. Makarova ◽  
Keyword(s):  
Heart Rate ◽  
Average Daily Gain ◽  
Natural Resistance ◽  
Control Group ◽  
General Tendency ◽  
Blood Samples ◽  
Newborn Calves ◽  
Daily Gain ◽  
Experimental Group ◽  
Observation Period

The article reflects observations on calves with different ways of keeping. Two groups of newborn calves were selected at the dairy complex (n=33). The calves of the control group were raised indoors in the traditional way in individual cages, and the experimental group was outdoors in the houses. The experiment was conducted from March to June. For the first 10 days, all calves were measured body temperature, pulse, respiration, blood samples for 1, 5, 10, 20 and 30 days of life were studied, incidence was taken into account. The heart rate in both groups gradually decreased by day 10. In the experimental group, this decrease was less pronounced and on average over the observation period, the pulse rate was 128 or 8 beats higher than in the control. The respiratory rate in calves of both groups had a general tendency to decrease. In experimental calves, this indicator was more constant than in controls. In calves of the experimental group, the average daily gain was 69.0 g higher, and the incidence was 14.9% lower compared to the calves of the control group. Authors established activation of metabolism, increase in natural resistance, weight gain and decrease in the incidence of disease in calves bred in the open air in individual houses.

Effect of parenteral administration of Selenium and vitamin E on health status of mammary gland and on selected antioxidant indexes in blood of dairy cows

2014 ◽  
Vol 17 (2) ◽  
pp. 217-223 ◽  
Author(s):  
F. Zigo ◽  
Z. Farkasóvá ◽  
J. Eleko ◽  
M. Lapin ◽  
M. Chripková ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Health Status ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Parenteral Administration ◽  
Control Group ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

scholarly journals Effect of Photobiomodulation Therapy on Oxidative Stress Markers in Healing Dynamics of Diabetic Neuropathic Wounds in Wistar Rats

2021 ◽  
Author(s):  
Gagana Karkada ◽  
G. Arun Maiya ◽  
Praveen Arany ◽  
Mohandas Rao ◽  
Shalini Adiga ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Photobiomodulation Therapy ◽  
Control Group ◽  
Sciatic Nerve Crush ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Experimental Group

Abstract Background Prolonged and overlapping phases of wound healing in diabetes are mainly due to the redox imbalance resulting in the chronicity of the wound. Photobiomodulation therapy works on the principle of absorption of photon energy and its transduction into a biological response in the living tissue. It alleviates the cellular responses, thereby improving the mechanism of wound healing in diabetes. Objective To find out the effect of photobiomodulation therapy of dosage 4 J/cm2 in the healing dynamics of diabetic neuropathic wounds in Wistar rats and its relation with oxidative stress markers. Methodology Diabetes was induced using Streptozotocin of 60 mg/kg of body weight to eighteen female Wistar rats. Neuropathy was induced by the sciatic nerve crush injury followed by an excisional wound of 2 cm2 on the back of the animal. Experimental group animals were treated with dosage 4 J/cm2 of wavelength 655 and 808 nm, and control group animals were kept unirradiated. The biomechanical, histopathological, and biochemical changes were analysed in both groups. Results There was a reduction in mean wound healing time and an increased rate of wound contraction in the experimental group animals compared to its control group. The experimental group showed improved redox status, and histopathological findings revealed better proliferative cells, keratinisation, and epithelialization than un-irradiated controls. Conclusions Photobiomodulation therapy of dosage 4 J/cm2 enhanced the overall wound healing dynamics of the diabetes-induced neuropathic wound and optimised the oxidative status of the wound, thereby facilitating a faster healing process.

scholarly journals Evaluation of stress in laboratory rabbits used for teaching purposes

2019 ◽  
Vol 88 (2) ◽  
pp. 249-255
Author(s):  
Monika Urbanová ◽  
Eva Kramářová ◽  
Jan Chloupek ◽  
Martina Najmanová
Keyword(s):  
Oryctolagus Cuniculus ◽  
Control Group ◽  
Health Examination ◽  
Pharmaceutical Sciences ◽  
Blood Samples ◽  
Habituation Process ◽  
Biochemical Indices ◽  
The University ◽  
And Control ◽  
Experimental Group

This study was intended as a contribution to the argument about possible suffering of animals used for demonstrative purposes during teaching at universities. Pharmacology lectures at the University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic were selected for this experiment. The study focused on stress assessment using haematological and biochemical indices. Blood samples were drawn from 20 rabbits (Oryctolagus cuniculus f. domesticus L.) 10 days after arrival at the institute (before the first practical lesson), and then in the 7th and 12th weeks of the semester with practical lessons. The haematological and biochemical indices were compared between the experimental group (n = 10) used for practical demonstrations and the control group (n = 10) which was kept in its hutch. Practical lessons included manipulation with rabbits, health examination, topical or total application and observation of the drugs’ effects. All the acts were carried out by students. Significant changes were detected in some of haematological (erythrocytes, haemoglobin, haematocrit) and biochemical (glucose, total protein, enzymes aspartate aminotransferase and lactate dehydrogenase) variables compared to the control group. The values obtained from the last blood sampling indicate a possible habituation process in the experimental group between the 8th and 12th week. The results were compared between the experimental and control group and also with the results of other studies with different stressors. In conclusion, the stress the rabbits used for teaching purposes at universities are exposed to, is tolerable.

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