Effect of parenteral administration of Selenium and vitamin E on health status of mammary gland and on selected antioxidant indexes in blood of dairy cows

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

Download Full-text

Adhesion of Malassezia pachydermatis of different growth type to canine epithelial cells

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0051 ◽

2014 ◽

Vol 17 (2) ◽

pp. 365-366 ◽

Cited By ~ 1

Author(s):

M. Florek ◽

J. Król ◽

Z. Staroniewicz ◽

B. Bażanów

Keyword(s):

Vitamin E ◽

Epithelial Cells ◽

Glutathione Peroxidase ◽

Dairy Cows ◽

Clinical Mastitis ◽

Control Group ◽

Growth Type ◽

Malassezia Pachydermatis ◽

Blood Samples ◽

Experimental Group

Download Full-text

Effects of Saccharomyces cerevisiae Addition in Dairy Cows Diets

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies ◽

10.15835/buasvmcn-asb:2020.0022 ◽

2021 ◽

Vol 78 (1) ◽

pp. 18

Author(s):

Marinela ENCULESCU

Keyword(s):

Saccharomyces Cerevisiae ◽

Health Status ◽

Dairy Cows ◽

Milk Yield ◽

Milk Fat ◽

Control Group ◽

Free Access ◽

Alternative Source ◽

Effective Measure ◽

Experimental Group

The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.

Download Full-text

Influence of increased lipid content in diet in the form of treated rapeseed meal on the metabolism and milk yield of dairy cows in the first third of lactation

Veterinární Medicína ◽

10.17221/5555-vetmed ◽

2012 ◽

Vol 51 (No. 6) ◽

pp. 346-355 ◽

Cited By ~ 5

Author(s):

A. Pechova ◽

R. Dvorak ◽

P. Drastich ◽

V. Lubojacka ◽

L. Pavlata ◽

...

Keyword(s):

Fatty Acids ◽

Vitamin E ◽

Dairy Cows ◽

Milk Yield ◽

Lipid Content ◽

Hdl Cholesterol ◽

Control Group ◽

Total Production ◽

Nonesterified Fatty Acids ◽

Experimental Group

The purpose of this study was to evaluate the influence of high lipid concentration in the diet, served as calcium salts of fatty acids from rape, on metabolism and the milk yield of dairy cows during the first third of lactation. 28 dairy cows were divided into experimental (E; n = 14) and control groups (C; n = 14) and monitored within 100 days of lactation since the day of parturition. The diet of both groups had a balanced content of energy and crude protein, while there was a difference in lipid content (C – 3.7% vs. E – 6.99% of dry matter in the diet). Blood and urine samples were taken at the end of 1st, 2nd and 3rd months of lactation. Evaluation of milk yield was carried out based on the results of monthly milk yield control, while the evaluation of reproduction was performed using data supplied by a farm livestock specialist. At the end of the first month, a higher degree of energetic metabolism disturbance was determined in group E in comparison with group C (beta-hydroxybutyrate 1.05 vs 0.51 mmol/l, P ≤ 0.05; nonesterified fatty acids 0.68 vs. 0.27 mmol/l, P ≤ 0.01), as well as a higher occurrence of liver damage (bilirubine 6.50 vs. 4.59 μmol/l, P ≤ 0,01; aspartate amino transferase 1.66 vs. 1.39 μkat/l, P≤ 0.05; lactate-dehydrogenase 45.2 vs. 34.3 μkat/l, P ≤ 0.05). During the entire experiment, the total concentration of cholesterol, HDL-cholesterol and vitamin E rose, and thus in the 3rd month, the values in the experimental group were almost double that of the control group (cholesterol 7.28 vs. 3.69 mmol/l, P ≤ 0.0001; HDL-cholesterol 5.43 vs. 3.26 mmol/l, P ≤ 0.0001; vitamin E 19.9 vs. 10.3 μmol/l, P ≤ 0.0001). The proportion of HDL-cholesterol was lower in group E (3rd month 76.1 vs. 88.8%, P ≤ 0.001). We also determined a higher total anti-oxidant status of serum in group E in the second (0.96 vs. 0.90 mmol/l, P ≤ 0.05) and third months of lactation (1.02 vs. 0.94 mmol/l, P ≤ 0.05), while other parameters of the anti-oxidation system (glutathionperoxidase, superoxiddismutase) did not differ between groups. The total production of milk within the 100 days of lactation in both monitored groups was similar. In group E the concentration of milk protein was lower (3.18 vs. 3.45%, P ≤ 0.01), while the concentration of fat was insignificantly higher (3.55 vs. 3.21%) than in group C. The results of effect on reproduction did not differ significantly either, but the total percentage of gravidity was higher in the experimental group. Our results revealed that feeding of higher doses of lipid (6.99 %) fed in bypass form during the first month after parturition creates the health risk of a fatty liver, but no negative impact on the health of dairy cows was demonstrated during the peak period of lactation.

Download Full-text

Salmonella Bacterin Vaccination Decreases Shedding and Colonization of Salmonella Typhimurium in Pigs

Microorganisms ◽

10.3390/microorganisms9061163 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1163

Author(s):

Eduarda Alexandra Gonçalves de Oliveira Moura ◽

Daniela Gomes da Silva ◽

Caio Henrique Turco ◽

Thainara Vitoria Carnevalli Sanches ◽

Gabriel Yuri Storino ◽

...

Keyword(s):

Salmonella Typhimurium ◽

Blood Count ◽

Control Strategies ◽

Control Group ◽

Blood Samples ◽

Commercial Vaccine ◽

Serum Igg ◽

And Control ◽

Experimental Group ◽

Over Time

Since the occurrence of swine salmonellosis has increased over time and control strategies other than biosecurity are highly recommended, the present study aimed to evaluate the efficacy of vaccination with Salmonella Choleraesuis and Salmonella Typhimurium bacterins in pigs. Two experimental groups were formed: G1, animals immunized with two doses of a commercial vaccine (n = 20); G2, control group (n = 20). After vaccination, all pigs were orally challenged (D0) with 108 CFU of Salmonella Typhimurium and evaluated for 40 days. Every 10 days after D0, five piglets from each experimental group were euthanized and submitted to the necroscopic examination, when organ samples were collected. Blood samples and rectal swabs were collected before the first dose of the vaccine (D−42), before the second dose (D−21), before the challenge (D0), and thereafter, every three days until D39. Blood count, serum IgG measurement by ELISA, and the excretion of Salmonella Typhimurium in feces were evaluated. While the results from blood count and serum IgG concentration did not differ, the detection and excretion of Salmonella between G1 and G2 differed (p < 0.05). Therefore, it was observed that this vaccine partially protected the animals against experimental infection with Salmonella Typhimurium, reducing the excretion of bacteria in feces.

Download Full-text

Microelement Supplementation in Dairy Cows by Mineral Lick

Acta Veterinaria Brno ◽

10.2754/avb200978010029 ◽

2009 ◽

Vol 78 (1) ◽

pp. 29-36

Author(s):

Štěpán Krys ◽

Eva Lokajová ◽

Aleš Podhorský ◽

Leoš Pavlata

Keyword(s):

Dairy Cows ◽

Selenium Concentration ◽

Control Group ◽

Dry Period ◽

Total Consumption ◽

Zn Concentration ◽

Ad Libitum ◽

Mn Concentration ◽

Experimental Group ◽

Mineral Lick

The aim of this experiment was to verify the option of providing microelement supplementation to dairy cows in the dry period through supplemented mineral lick, and then to compare the content of microelements in the blood, colostrum and milk. The experiment was carried out on a farm with Czech Fleckvieh dairy cows. We formed an experimental group (E), supplied with mineral lick ad libitum enhanced with organic forms of microelements (Se, Zn, Cu, Mn) for four to five weeks before parturition and 1 week after parturition. The control group (C) did not receive any mineral lick. The use of mineral lick by cows was very uneven, its total consumption oscillated between 0 to 250 g of lick per animal per day. Lick feeding did not result in evidential increase of concentrations of monitored microelements in the cows’ blood, colostrum and milk. Only a tendency to increased concentrations of Se and Cu in the blood of group E of cows was observed; the average Se concentration increased by 60 μg l-1 (Cu by 9 μmol l-1), whereas in group C, Se increased only by 30 μg l-1 (Cu by 6 μg l-1). The concentration of microelements in colostrum after parturition dropped quickly. Significant drops were determined for Se, Cu and Zn on the first (for Mn on the second) day after parturition. Selenium concentration in the first colostrum was 5 times higher than in milk, Cu concentration 3 times higher, Zn concentration 4 times higher and Mn concentration 1.3 times higher.

Download Full-text

Biotechnological aspects of transformation of heavy metal salts and their detoxication in milk of milk cows

E3S Web of Conferences ◽

10.1051/e3sconf/202126202010 ◽

2021 ◽

Vol 262 ◽

pp. 02010

Author(s):

Boris Dzagurov ◽

Oleg Getokov ◽

Vladimir Gukezhev ◽

Taladin Kokov ◽

Vitaly Vorokov

Keyword(s):

Heavy Metals ◽

Dairy Cows ◽

Particle Diameter ◽

Bentonite Clay ◽

The Body ◽

Control Group ◽

Milk Cows ◽

Heavy Metal Salts ◽

Cadmium Lead ◽

Experimental Group

Based on the results of previous studies on pigs and poultry, in which encouraging results of a significant decrease in the concentration of heavy metals in the body were obtained, respectively, in pork, poultry and eggs, the study provided for the use of the bentonite clay of the Zamankul deposit (RNO-Alania) as an enterosorbent in relation to heavy metals and detoxification of the organism of dairy cows. In order to study the feasibility of using bentonite as an enterosorbent, studies were performed on dairy cows of the Simmental breed in the Kaloyev farm located in st. Zmeyskaya, Kirovsky district of North Ossetia-Alania. To conduct research on the principle of analog pairs, two experimental groups of cows (control and experimental, five cows in each group) of the 3rd and 4th lactation were formed. The experiment lasted for 305 days of lactation from March 2018 to January 2019. Herewith, the control group of cows was fed with a basic diet balanced in all nutritional elements, the experimental livestock, together with the main diet, daily in the composition of the concentrates was injected with crushed bentonite with a particle diameter of 4-6 mm based on the dry matter of the feed (137 g/animal). The study of the concentration of heavy metals (cadmium, lead and zinc) in soil, feed, blood and milk was performed in two periods of lactation (on the 250th and 300th days of lactation). Herewith, an increased content of heavy metals in the soil was established, relative to the MPL: cadmium - 10.1; lead - 7.4; zinc - 9.7 times more, in drinking water: cadmium - 2.5 times; lead - 9.0 times and zinc - 9.7 times more, in feed: cadmium - from 1.7 to 5.3 times; lead - from 1.1 to 1.7; zinc - from 1.0 to 2.5 times the MPL. The inclusion of bentonite to the ration of the animals of the experimental group, in comparison with the control group, in both study periods (250 and 300 days of lactation) contributed to a significant decrease in the concentration of the studied heavy metals in the blood from 17 to 20%, in milk - from 16 to 18 %. The transformation ration values of heavy metals from feed into the body, respectively, into milk, were also lower in the animals of the experimental group, relative to the control.

Download Full-text

ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ ТА АКТИВНІСТЬ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ У КРОВІ КОРОПІВ ЗА ДІЇ РІЗНОГО РІВНЯ ВІТАМІНУ Е І СЕЛЕНУ В РАЦІОНІ

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7146 ◽

2016 ◽

Vol 18 (3(71)) ◽

pp. 201-204

Author(s):

S.V. Yurchak ◽

O.V. Derenj ◽

O.I. Vishchur ◽

Yu.M. Zabytivskyi

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Control Group ◽

Fish Feed ◽

Dose Dependent Decrease ◽

Fat Soluble Vitamins ◽

And Control ◽

Dose Dependent ◽

Experimental Group ◽

Different Levels

The article consist data about effect of different levels of vitamin E and selenium in the diet of carps during their growing and also informationabout the influence on processes of lipid peroxidation and activity of antioxidant protection in their body.The experiment conducted in three experimental ponds. After wintering there were placed four ears mature females and males carp, six individuals in each group. Supplements of vitamin E administered at a rate of 25 mg/kg and drug «Sel–Plex» the rate of selenium – 0.3 mg/kg were added to further basic diet(BD) of female and male carp first experimental group (EG1). The second experimental group (EG2) received (BD) and vitamin E supplements in an amount of 75 mg / kg, and just as in EG1 – selenium – 0.3 mg/kg of feed.The control group received fish feed without additives vitamins and minerals.Feeding lasted for 30 days, due to the physiological needs of the fish feed. After spawning in fish of research and control group swere taken blood samples for biochemical research.The study led to a dose–dependent decrease (p < 0.01 – 0.001) content of TBA–active products and hydroperoxidase of lipids, but did not significantly effect onsuperoxide dismutase and glutathione peroxidase activity of blood So, the reduction of lipid peroxidation products in the carp’s blood of experimental groups probably was caused by the growth of non–enzymatic level, which is associated with fat–soluble vitamins.

Download Full-text

The role of rhaponticum carthamoides in feeding of dairy cows

Siberian Herald of Agricultural Science ◽

10.26898/0370-8799-2019-5-7 ◽

2019 ◽

Vol 49 (5) ◽

pp. 52-60

Author(s):

V. A. Voloshin ◽

D. A. Matolinets ◽

N. A. Morozkov ◽

G. P. Maysak

Keyword(s):

Dairy Cows ◽

Dry Matter ◽

Positive Impact ◽

Sugar Content ◽

The Body ◽

Metabolic Energy ◽

Control Group ◽

Metabolic Processes ◽

Rhaponticum Carthamoides ◽

Experimental Group

The results of studies of the use of rhaponticum carthamoides for feeding dairy cows are presented. The experiment was carried out on Holstein cows of black-and-white breed in Perm Territory. Vitaminherbal fl our from green mass of rhaponticum carthamoides at a dose of 0.4 and 1.0 kg per head per day in the period of 10-16 days before calving and in the fi rst 30 days of lactation was introduced into the feeding rations of two experimental groups of cows. High sugar content in rhaponticum carthamoides (from 4.15 to 11.2% in dry matter and metabolic energy from 10.87 to 12.12 MJ/ kg) was noted. In the studied material from this plant, the content of the 20-hydroxyecdysone was 0.49% of active ingredients in the dry matter of the product at a rate of 0.25%. The introduction of rhaponticum carthamoides in the diet of feeding animals contributed to the increase in the intensity of metabolic processes in the body. A gradual increase in cholesterol levels in all experimental groups was noted. The most signifi cant growth was observed in cows of the second experimental group by 0.82 mmol / l or 29.50% (p <0.01). Compared with the fi rst experimental group, the increase was 0.13 mmol / l or 5.2%, and the control group – 0.36 mmol / l or 14.17%. Stimulation of metabolic processes in the body of cows of the experimental groups had a positive impact on reproduction, which was manifested in the reduction of the service period duration, compared with the control group.

Download Full-text

To Evaluate the Effect of Vitamin E Therapy on the Oxidative Stress Markers (Nitric Oxide, SOD, Glutathione Peroxidase) & Vitamin E Levels in Pulmonary Tuberculosis Patients

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2020/651 ◽

2020 ◽

Vol 9 (40) ◽

pp. 2970-2975

Author(s):

Rohit John Chaudhary ◽

Bharti Kwatra Uppal

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Vitamin E ◽

Glutathione Peroxidase ◽

Pulmonary Tuberculosis ◽

Older Age ◽

Control Group ◽

Ros Production ◽

Age Group ◽

Sod Activity

BACKGROUND Severe oxidative stress has been reported in TB patients because of infection associated with malnutrition and poor immunity. Mycobacteria can induce reactive oxygen species (ROS) production by activating phagocytes, and enhanced ROS production may promote tissue injury and inflammation. We wanted to compare the effect of antioxidant administration in the outcome of ATT treatment between the test and the control group. METHODS This perspective study was conducted in the Departments of Biochemistry and Chest Medicine, CMC & Hospital. Hundred patients (fifty controls and fifty tests) who were diagnosed as pulmonary tuberculosis and started on DOT therapy under RNTCP during this period were included in the study. Each participant in the study was subjected to the following test at the first visit, 2nd month and 6th month follow up (biochemical markers Nitric oxide, SOD, Glutathione Peroxidase and Vitamin E levels). Statistical analysis was done using SPSS version. RESULTS The results were based on four categories (male / female, alcoholic / non-alcoholic, smoker / non-smoker, and younger / older age group). Females had responded better with greater fall in percentage of nitric oxide values (69 %) than males (64.1 %). The mean of SOD activity (277.5 + / - 31.5) was more in smokers than non-smokers (261.3 + / - 36.0) & percentage fall of nitric oxide in smokers (65 %) & non-smokers (67 %). In alcoholics the percentage fall of nitric oxide (68.3 %) was higher with more SOD activity (Mean 278.7 + / - 27.6) than non-alcoholics (Mean 256 + / - 38.0) indicating a positive correlation of smoking & alcoholism with tuberculosis. Younger age group responded better with more fall in the percentage of nitric oxide (67 %) & mean SOD activity (265.8 + / - 30.1) than older age group. CONCLUSIONS Antioxidant supplementation reduces oxidative stress, improves the effectiveness of ATT therapy, and thus helps in improving the outcome in pulmonary tuberculosis. KEY WORDS Pulmonary TB, ATT (Anti-Tubercular Treatment), Antioxidants & Free Radicals

Download Full-text

Pengaruh pemberian probiotik Lactobacillus helveticus Rosell-52 dan Lactobacillus rhamnosus Rosell-11 terhadap kadar limfosit lanjut usia

JURNAL GIZI INDONESIA ◽

10.14710/jgi.3.1.102-108 ◽

2014 ◽

Vol 3 (1) ◽

pp. 13-19

Author(s):

Retno Wahyuningsih ◽

Darmono SS ◽

Ani Margawati

Keyword(s):

Health Status ◽

Vitamin E ◽

Vitamin A ◽

Vitamin C ◽

Lactobacillus Rhamnosus ◽

The Elderly ◽

Lactobacillus Helveticus ◽

Control Group ◽

Quality Of Sleep

Background: Infectious disease remains a health problem for the elderly. The elderly are susceptible to infection,because the body's ability to fight infection decreases immunity. The immune status can be improved through the effortsof immunization, and nutrition. However the elderly are not sufficient to meet the nutritional needs of food, so probioticcould support nutritional adequacy.Objective: Analyze the effect of probiotic Lactobacillus helveticus Rosell-52 and Lactobacillus rhamnosus Rosell-11 onlevels of lymphocytes in the elderly in Tresna Elderly Social Institution Puspakarma Mataram, West Nusa Tenggara.Methods: This was quasi-experimental study, randomized pre-post control group design. The population is elderly aged60-75 years, involving 45 subjects were divided randomly into two groups, namely the treatment groups givenprobiotics and the control group was given a placebo. Probiotics were given for 4 weeks, with the provision of onecapsule a day. Immunity status in this study was lymphocytes. Data examined included the intake of nutrients (protein,vitamin A, vitamin C, vitamin E, Fe, Zn), health status, quality of sleep, and levels of lymphocytes in the elderly. Datawere analyzed using bivariate with different test Independent sample t-test, Mann-Whitney U test, Spearman, Pearson,and Paired t-test.Results: Increasing number of lymphocytes were significantly in the treatment group (χ ± SD: 1.84 ± 1.85) and in thecontrol group (0.95 ± 5.0). There is no relationship between intake (protein, vitamin A, vitamin C, vitamin E, Fe, Zink),physical health status, and quality of sleep with levels of lymphocytes.Conclusion: Probiotics in 4 weeks is proven to increase the number of lymphocytes in the elderly.

Download Full-text