scholarly journals Polycaprolactone Foam Functionalized With Chitosan Microparticles – a Suitable Scaffold for Cartilage Regeneration

2016 ◽  
pp. 121-131 ◽  
Author(s):  
E. FILOVÁ ◽  
B. JAKUBCOVÁ ◽  
I. DANILOVÁ ◽  
E. KUŽELOVÁ KOŠŤÁKOVÁ ◽  
T. JAROŠÍKOVÁ ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Growth ◽  
Dna Content ◽  
Viscoelastic Properties ◽  
Cartilage Regeneration ◽  
Biomechanical Properties ◽  
Porous Scaffolds ◽  
Chitosan Microparticles ◽  
Salt Leaching ◽  
Cell Growth And Differentiation

For biodegradable porous scaffolds to have a potential application in cartilage regeneration, they should enable cell growth and differentiation and should have adequate mechanical properties. In this study, our aim was to prepare biocompatible scaffolds with improved biomechanical properties. To this end, we have developed foam scaffolds from poly-Ɛ-caprolactone (PCL) with incorporated chitosan microparticles. The scaffolds were prepared by a salt leaching technique from either 10 or 15 wt% PCL solutions containing 0, 10 and 20 wt% chitosan microparticles, where the same amount and size of NaCl was used as a porogen in all the cases. PCL scaffolds without and with low amounts of chitosan (0 and 10 wt% chitosan) showed higher DNA content than scaffolds with high amounts of chitosan during a 22-day experiment. 10 wt% PCL with 10 and 20 wt% chitosan showed significantly increased viscoelastic properties compared to 15 wt% PCL scaffolds with 0 and 10 wt% chitosan. Thus, 10 wt% PCL scaffolds with 0 wt% and 10 wt% chitosan are potential scaffolds for cartilage regeneration.

Characteristics and Cell Growth Behavior of Gelatin/Polyvinyl Alcohol Porous Scaffolds

2021 ◽  
Vol 13 (5) ◽  
pp. 883-888
Author(s):  
Soyeun Kim ◽  
Deuk Yong Lee ◽  
Bae-Yeon Kim ◽  
Jung In Yoon
Keyword(s):  
Mechanical Properties ◽  
Compressive Strength ◽  
Cell Growth ◽  
Polyvinyl Alcohol ◽  
Growth Behavior ◽  
Porous Scaffolds ◽  
Freeze Thaw ◽  
Swelling Rate ◽  
G Ratio ◽  
Acetal Group

Gelatin/polyvinyl alcohol (PVA) (g/P) porous scaffolds with a glutaraldehyde/g ratio of 3 were synthesized by freeze/thaw and lyophilization to evaluate the effect of the g/P ratio (10/0 to 7/3) on the mechanical properties and cell growth behavior of the scaffolds. Fourier transform infrared spectroscopy results showed that the g/P scaffolds exhibited good crosslinking between gelatin and gelatin (imine), PVA and PVA (acetal group), and gelatin and PVA (imine). The pore size decreased gradually from 170±63 μm, to 140±60 μm, 129±64 μm, and 74 ±23 μm by varying the g/P ratio from 10/0, to 9/1, 8/2, and 7/3, respectively. As the g/P ratio was changed from 10/0 to 7/3, the compressive strength and swelling rate increased gradually from 150± 17 kPa to 290 ±28 kPa and 1268± 11% to 1640 ±35%, respectively. Among the scaffolds, 7/3 g/P scaffolds with tailored properties are suitable for wound healing.

scholarly journals Design and Characterization of Sheet-Based Gyroid Porous Structures with Bioinspired Functional Gradients

Materials ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 3844
Author(s):  
Yuan Jin ◽  
Haoyu Kong ◽  
Xueyong Zhou ◽  
Guangyong Li ◽  
Jianke Du
Keyword(s):  
Mechanical Properties ◽  
Physical And Mechanical Properties ◽  
Biomechanical Properties ◽  
Potential Method ◽  
Structural Features ◽  
Design Parameters ◽  
Porous Scaffolds ◽  
Porous Structures ◽  
Compression Tests ◽  
Graded Structures

A new type of sheet porous structures with functionally gradients based on triply periodic minimal surfaces (TPMS) is proposed for designing bone scaffolds. The graded structures were generated by constructing branched features with different number of sheets. The design of the structure was formulated mathematically and five types of porous structure with different structural features were used for investigation. The relative density (RD) and surface area to volume (SA/V) ratio of the samples were analyzed using a slice-based approach to confirm their relationships with design parameters. All samples were additively manufactured using selective laser melting (SLM), and their physical morphologies were observed and compared with the designed models. Compression tests were adopted to study the mechanical properties of the proposed structure from the obtained stress–strain curves. The results reveal that the proposed branched-sheet structures could enhance and diversify the physical and mechanical properties, indicating that it is a potential method to tune the biomechanical properties of porous scaffolds for bone tissue engineering (TE).

scholarly journals Physical, thermal, and mechanical properties of highly porous polylactic acid/cellulose nanofibre scaffolds prepared by salt leaching technique

2021 ◽  
Vol 10 (1) ◽  
pp. 1469-1483
Author(s):  
Revati Radakisnin ◽  
Mohd Shukry Abdul Majid ◽  
Mohd Ridzuan Mohd Jamir ◽  
Mohd Faizal Mat Tahir ◽  
Cheng Ee Meng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polylactic Acid ◽  
Mechanical Performance ◽  
Cell Attachment ◽  
Composite Scaffold ◽  
Average Pore Size ◽  
Pennisetum Purpureum ◽  
Porous Scaffolds ◽  
Average Pore ◽  
Salt Leaching

Abstract This study aimed to prepare and characterise polylactic acid (PLA) reinforced with cellulose nanofibre (CNF) from a Pennisetum purpureum-based composite scaffold and determine its structural and mechanical properties. Porous scaffolds with CNF compositions of 5‒20 wt% in the PLA matrix were developed using solvent casting and particulate leaching of its porogen at 90 wt% of loadings. Morphology studies using field emission scanning electron microscopy revealed that the scaffolds had well-interconnected pores with an average pore size range of 67‒137 µm and porosity >76%. X-ray diffraction confirmed the interconnectivity and homogeneity of the pores and the fibrous structure of the scaffolds. The compressive strength of the fabricated scaffolds varied between 2.34 and 6.66 MPa, while their compressive modulus was between 1.95 and 6.04 MPa for various CNF contents. Furthermore, water absorption and thermal degradation studies showed that the scaffold had good hydrophilicity and improved thermal stability. These findings highlight the need to modify the pore structure and mechanical performance simultaneously for tissue engineering. Thus, this study concludes that the developed PLA scaffolds reinforced with CNF from Pennisetum purpureum are potential candidates for cell attachment and extracellular matrix generation.

Biomechanical Properties of the Stifle Joint Lateral Collateral Ligament in Dogs

1992 ◽  
Vol 05 (04) ◽  
pp. 158-162 ◽  
Author(s):  
D. Blackketter ◽  
J Harari ◽  
J. Dupuis
Keyword(s):  
Mechanical Properties ◽  
Cruciate Ligament ◽  
Lateral Collateral Ligament ◽  
Biomechanical Properties ◽  
Fibular Head ◽  
Joint Stability ◽  
Collateral Ligament ◽  
Cranial Cruciate Ligament ◽  
Stifle Joint

Bone/lateral collateral ligament/bone preparations were tested and structural mechanical properties compared to properties of cranial cruciate ligament in 15 dogs. The lateral collateral ligament has sufficient stiffness to provide stifle joint stability and strength to resist acute overload following fibular head transposition.

The Effect of Crosslinking Agents on the Properties of Type II Collagen Biomaterials

2021 ◽  
Vol 57 (4) ◽  
pp. 166-180
Author(s):  
Maria-Minodora Marin ◽  
Madalina Georgiana Albu Kaya ◽  
George Mihail Vlasceanu ◽  
Jana Ghitman ◽  
Ionut Cristian Radu ◽  
...  
Keyword(s):  
Research Work ◽  
Cartilage Tissue Engineering ◽  
Chemical Properties ◽  
Cartilage Regeneration ◽  
Type Ii Collagen ◽  
Biomechanical Properties ◽  
Cartilage Tissue ◽  
Cross Linking ◽  
Type Ii ◽  
Crosslinking Agents

Type II collagen has been perceived as the indispensable element and plays a crucial role in cartilage tissue engineering. Thus, materials based on type II collagen have drawn farther attention in both academic and research for developing new systems for the cartilage regeneration. The disadvantage of using type II collagen as a biomaterial for tissue repairing is its reduced biomechanical properties. This can be solved by physical, enzymatic or chemical cross-linking processes, which provide biomaterials with the required mechanical properties for medical applications. To enhance type II collagen properties, crosslinked collagen scaffolds with different cross-linking agents were prepared by freeze-drying technique. The present research work studied the synthesis of type II collagen biomaterials with and without crosslinking agents. Scaffolds morphology was observed by MicroCT, showing in all cases an appropriate microstructure for biological applications, and the mechanical studies were performed using compressive tests. DSC showed an increase in denaturation temperature with an increase in cross-linking agent concentration. FTIR suggested that the secondary structure of collagen is not affected after the cross-linking; supplementary, to confirm the characteristic triple-helix conformation of collagen, the CD investigation was performed. The results showed that the physical-chemical properties of type II collagen were improved by cross-linking treatments.

scholarly journals Porous scaffolds with the structure of an interpenetrating polymer network made by gelatin methacrylated nanoparticle-stabilized high internal phase emulsion polymerization targeted for tissue engineering

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22544-22555
Author(s):  
Atefeh Safaei-Yaraziz ◽  
Shiva Akbari-Birgani ◽  
Nasser Nikfarjam
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Polymer Network ◽  
Synthetic Polymers ◽  
Tissue Scaffolds ◽  
Interpenetrating Polymer Network ◽  
Porous Scaffolds ◽  
Promising Strategy ◽  
Internal Phase

The interlacing of biopolymers and synthetic polymers is a promising strategy to fabricate hydrogel-based tissue scaffolds to biomimic a natural extracellular matrix for cell growth.

scholarly journals Age-dependent and radial sectional differences in the dynamic viscoelastic properties of bamboo culms and their possible relationship with the lignin structures

2020 ◽  
Vol 66 (1) ◽  
Author(s):  
Yoko Okahisa ◽  
Keisuke Kojiro ◽  
Hatsuki Ashiya ◽  
Takeru Tomita ◽  
Yuzo Furuta ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Viscoelastic Properties ◽  
Structural Variation ◽  
Thermal Softening ◽  
Peak Temperature ◽  
Outer Side ◽  
Phyllostachys Pubescens ◽  
Age Dependence ◽  
Age Dependent ◽  
S Peak

Abstract Age is an important factor that dictates bamboo’s mechanical properties. In Japan, bamboo plants aged 3–5 years are selected for use as materials because of their robustness and decorative or craft-friendly characteristics. In this study, the age-dependent and radial sectional differences in bamboo’s dynamic viscoelastic properties in relation to lignin structural variation, were evaluated. We used Phyllostachys pubescens samples at the current year and at 1.5, 3.5, 6.5, 9.5, 12.5, and 15.5 years of age. There was a clear age dependence in the peak temperature of tan δ and in the yield of thioacidolysis products derived from β-O-4 lignin structures. The highest peak temperature tan δ value was detected in 3.5-year-old bamboo, which contained the highest amount of the thioacidolysis products. Moreover, tan δ’s peak temperature was always higher on the outer side, and the ratio of S/G thioacidolysis products was always higher on the inner side of bamboo plants of all ages. These results suggest that changes in bamboo’s thermal softening properties from aging are caused by the maturation and degradation of lignin in bamboo.

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