scholarly journals Effect of selenium applied to pasture on the selenium status of grazing sheep

1999 ◽  
pp. 69-73
Author(s):  
A.M. Moorhouse ◽  
C.T. Westwood ◽  
A.J. Dumbleton ◽  
L.P. Donnelly ◽  
L.A. Bridger
Keyword(s):  
Slow Release ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Selenium Treatment ◽  
Second Year ◽  
Highly Correlated ◽  
Selenium Supplements

Blood selenium concentrations and liveweight gains were assessed for sheep grazing pastures treated with different selenium supplements over a 2-year period. Three 0.5 hectare irrigated blocks with pasture selenium concentrations of less than 0.04 μg/g DM were identified near Lincoln, Canterbury during 1997. Treatments applied in early November 1997 were: (1) 1 kg/ha of an experimental selenium product (SRSe5, containing 10g selenium); (2) 1 kg/ha of a commercially available slow release selenium product (positive control, containing 10g selenium) and (3) nil selenium treatment (negative control); all treatments used 60 kg urea/ha as the carrier. Treatments were stocked at 30 lambs/ha in November 1997. Liveweight gains and concentrations of blood selenium were monitored fortnightly for the first 4 months of the study and monthly thereafter. Following mating in April 1998, blood selenium concentrations and liveweight gains of spring born progeny were assessed. Ewes grazing the SRSe5 and positive control treatments had significantly greater concentrations of blood selenium than the negative control group and the concentrations for the SRSe5 group were significantly greater than from the positive control. Negative control ewes and their lambs were removed from the study at lambing due to low blood selenium concentrations and concerns regarding their health. Concentrations of blood selenium did not differ significantly between lambs of SRSe5 and positive control ewes born in the second year of the study. Lamb and ewe blood selenium concentrations were highly correlated. Liveweight gains did not differ significantly between SRSe5, positive control or negative control groups for ewes or lambs during either year of the study. This study demonstrated that the application of selenium to pastures is a highly effective longterm strategy by which concentrations of blood selenium may be elevated in sheep under New Zealand pastoral conditions. While liveweights did not differ significantly between treatments during either year of the study, the small numbers of animals used limited the power with which differences could be detected between treatments. Keywords: pasture, selenium, sheep, supplementation, topdressing

scholarly journals In Ovo β-Alanin Solüsyonu Alan Yeni Çıkmış Etlik Piliç Civcivlerinde Kuluçka Randımanı, Bazı Kuluçka Parametreleri, Kalite Skoru ve Yaşama Gücü

2018 ◽  
Vol 6 (10) ◽  
pp. 1469
Author(s):  
Canan Kop Bozbay ◽  
Ahmet Akdağ ◽  
Helin Atan ◽  
Nuh Ocak
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Quality Score ◽  
Control Group ◽  
Control Groups ◽  
In Ovo ◽  
Broiler Breeder ◽  
Death Rates ◽  
Chick Quality

This study was conducted to evaluate the effects of In ovo injection of β-Alanine which is a modified form of the alanine amino acid on hatchability, hatching weight, chick quality score and survival in broiler breeder eggs. For this purpose, 120 fertile eggs from Ross 308 breeder (32-week-old) were allocated randomly to four groups with 3 replicates. Except the negative control group eggs (non injected; NC), to the amniotic fluid of the eggs in the other 3 groups were injected with 1 ml solutions 0.9% salt (Positive control, PK), 0.75% (0.75βA) or 1.5% (1.5βA) by using 19 mm and 27 gauge needle. Death rates at the different stages of embryonic development, hatching yield, weights and quality scores of chicks and mortalities were recorded at hatching day. Hatching weight of the chicks in the 0.75βA group was higher than other two control groups. Accessing feed, determined as an indicator of chick quality, was better in the 0.75βA group. Conversely, mobility score of chicks in the NC group was higher than other groups. Hatching yield, chick quality score and mortalities of the first week were not affected from In ovo βA injection. Results of this study demonstrated that In ovo βA injection can be used up to 1.5% without any deteriorations on weight, quality and mortalities of chicks but 0.75% βA injection induced better hatching characteristics.

scholarly journals Natural plant extracts and prebiotic compounds as alternatives to antibiotics in broiler chicken diets in a necrotic enteritis challenge model

2013 ◽  
Vol 53 (12) ◽  
pp. 1247 ◽  
Author(s):  
J. K. Vidanarachchi ◽  
L. L. Mikkelsen ◽  
C. C. Constantinoiu ◽  
M. Choct ◽  
P. A. Iji
Keyword(s):  
Broiler Chickens ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Control Group ◽  
Soluble Carbohydrate ◽  
Necrotic Enteritis ◽  
Control Groups

An experiment was conducted to determine the effects of two different water-soluble carbohydrate extracts (renga renga lily extract and Acacia extract), and two commercially available prebiotic compounds, Fibregum and Raftifeed-IPE, on the performance of broiler chickens subjected to a necrotic enteritis (NE) challenge model. These treatments were compared with negative control and a positive (Zn-bacitracin) control treatments. An overall 8.8% NE-related mortality was recorded, with mean jejunal and ileal lesion scores in dead birds ranging from 3.03 to 3.90 in all challenged groups except the positive control groups. NE-specific deaths or clinical abnormalities were not observed with unchallenged control and positive control groups. At 7 days post-challenge, the concentration of specific IgY antibodies against the α-toxin of Clostridium perfringens in the serum was lower (P < 0.05) in birds fed the positive control and Fibregum-supplemented diets than in the negative control group. However, birds fed Fibregum had increased (P < 0.05) IgM concentration compared with those fed Acacia extract and lily extract. The Fibregum-fed group also had higher (P < 0.05) IgA concentrations in serum than did the positive-control and lily extract-supplemented groups at 14 days but this effect did not persist to 21 days. The results from the present study demonstrated that supplementation with water-soluble carbohydrates from two plant sources was not effective in controlling NE. However, the prebiotic compound Fibregum was found to be having some immunomodulatory effects. Addition of Zn-bacitracin and monensin was highly effective in counteracting the negative effects of the disease challenge.

scholarly journals Evaluation of the effect of different concentrations of Arsenicum album 6cH on intoxicated rats

2021 ◽  
Vol 8 (28) ◽  
pp. 119-127
Author(s):  
Olney Leite Fontes ◽  
Fátima Cristiane Lopes Goularte Farhat ◽  
Amarilys Toledo Cesar ◽  
Marilisa Guimarães Lara ◽  
Maria Imaculada Lima Montebelo ◽  
...  
Keyword(s):  
Atomic Absorption Spectroscopy ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Before And After ◽  
Male Wistar Rats

Aims: Homeopaths diverge on the concept of dose, i.e. the amount of drug that a patient must take to alter his or her state of disease. In order to stimulate reflections on this concept, this study sought to evaluate in vivo the effect of different concentrations of Arsenicum album 6cH prepared according to homeopathic pharmacotechnics. Methods: male Wistar rats were intoxicated with arsenic and then treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1%, administered orally. The amount of arsenic retained in the animals’ organism and that eliminated by urine were measured through atomic absorption spectroscopy. Samples of urine were collected before and after intoxication and during treatment. The positive control group (intoxicated animals) and the negative control group (non-intoxicated animals) received only the vehicle used in the preparation of the medicine. Results: Groups treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1% eliminated significant amounts of arsenic when compared to the control groups. The group treated with Arsenicum album 6cH eliminated significantly higher amounts of arsenic than the group treated with the diluted medicine at 1%. Conclusion: results suggest that Arsenicum album 6cH should not be diluted as not to compromise its effectiveness in the treatment of rats intoxicated with arsenic.

scholarly journals The Role of Ceftazidime as a Photosensitizer in Human Erythrocytes Through Oxidative Stress Mechanism

2018 ◽  
Vol 10 (2) ◽  
pp. 128-32 ◽  
Author(s):  
Mashuri Mashuri ◽  
Mustofa Ruhullah ◽  
Bayu Diertama Putera ◽  
Vicky Pramudinta Mega ◽  
Fadillah Alma Putra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbonyl Compounds ◽  
Superoxide Radical ◽  
Uv Light ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups

BACKGROUND: Ceftazidime was known to cause photosensitization reactions. However, how it plays the role remained unclear. In our study, we aim to investigate the photosensitization effect of ceftazidime in erythrocytes via oxidative stress.METHODS: Samples were divided into six different groups: negative control group, positive control group and four experimental groups with 10%, 20%, 30%, and 40% ceftazidime, respectively. The positive control and experimental groups were exposed to ultraviolet (UV)-light for 2 hours. Superoxide radical, malondialdehyde (MDA), carbonyl compounds (CC) and methemoglobin (Met-Hb) levels were then measured.RESULTS: The results showed a significant increased of superoxide radical, MDA, CC and Met-Hb levels in all experimental groups compared to both negative or positive control groups (p< 0.05).CONCLUSION: In conclusion, our study confirmed the role of ceftazidime as a photosensitizer in erythrocytes via the oxidative stress mechanisms.KEYWORDS: ceftazidime, oxidative stress, photosensitizer, photosensitization.

scholarly journals The effectiveness of Boiled figs leaf (FIcus Carica L) and Rosy Periwinkle (Catharantus roseus) on SGOT and SGPT levels of male wistar strain rats with hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 850-859
Author(s):  
Debora Oktaviani
Keyword(s):  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Wistar Strain ◽  
Significant Difference

Hepatitis is an inflammatory disease of liver cells that is contagious in the low category, the cause of it is bacteria, viruses, drugs and alcohol. The purpose of this study was to determine the effectiveness of boiled figs leaf (Ficus Carica L) and Rosy periwinkle (Catharantus roseus ) in reducing SGOT and SGPT levels. The subjects of this study were 30 male Wistar strain rats with age of 2-3 months, body weight 180-200 grams. Rats were divided into 3 groups; treatment groups, negative control groups and positive control groups. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. The treatment group was given 0.4 grams boiled figs leaf (Ficus Carica L) and 2.6 grams boiled rosy periwinkle  (Catharantus roseus) it given as much as 3.6 cc / day orally for 7 days. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare SGOT and SGPT levels. The results showed there were significant differences in SGOT levels between the treatment group, the positive control group, and the negative control group (p <0.05) and there was a significant difference in the SGPT value between the treatment group and the positive control group (p <0.05), while the negative control group and the treatment group there was no significant difference (p = 0.75). The conclusion of this study was the water extraction of figs leaf (Ficus Carica L), Rosy periwinkle (Catharantus roseus) had an effect in decreasing the SGOT and SGPT values of male wistar strain rats induced by paracetamol.

The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 34
Author(s):  
Arya Barahmanta ◽  
Muhammad Faizal Winaris ◽  
Pambudi Raharjo
Keyword(s):  
Hyperbaric Oxygen ◽  
Oxygen Therapy ◽  
Tooth Movement ◽  
Bone Remodelling ◽  
Orthodontic Tooth Movement ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Pressure Area

<p><strong><em>Background:</em></strong><em> Orthodontic tooth movement is a </em><em>interaction prosess</em><em> of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO).  <strong>Purpose: </strong>To determine the difference number  of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. <strong>Materials and Methods</strong>: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male)  were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. <strong>Result:</strong> The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than  negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest  than positive control group (K+=3,83) and treatment (P=3,25). <strong>Conclusion: </strong>Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.</em></p><pre><strong> </strong></pre><p><strong><em>Keywords:</em></strong><em> Hyperbaric Oxygen, Tooth movement, Bone remodeling, </em><em>Osteocyte</em><em></em></p><p><em> </em></p><p><strong><em>Correspondence:</em></strong><em> </em><em>Arya Brahmanta</em><em>, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email:</em><em> </em><a href="mailto:[email protected]"><em>arya.brahmanta</em><em>@</em><em>hangtuah.ac.id</em></a></p>

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

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