BLV early diagnosis - key to successful farm recovery

2020 ◽  
pp. 8-12
Author(s):  
Anatoliy M. Kovalenko ◽  
◽  
Nazar V. Yavnikov ◽  
Maksim V. Petropavlovskiy ◽  
Albina G. Isaeva ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Infectious Diseases ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
Polymerase Chain Reaction Method ◽  
Agricultural Enterprises ◽  
Republic Of Bashkortostan ◽  
Serological Studies ◽  
Monitoring And Forecasting ◽  
The Republic

The aim of the research is to use the early diagnosis of cattle leukemia virus-infected animals using serological and molecular genetic methods, as well as to determine the significance of PCR in detecting BLV infected calves. The studies were carried out in the leukemia laboratory of the department for monitoring and forecasting of infectious diseases of the Ural Federal Agrarian Research Center, at the department of infectious and invasive pathology of the Belgorod State Agrarian University named after V.Ya. Gorin, as well as in 20 livestock agricultural enterprises of the Tyumen, Chelyabinsk, Kurgan, Belgorod regions and the Republic of Bashkortostan. The studies were carried out in the framework of the 160th direction of the FNI Program of State Academies of Sciences for 20132020 on the topic «Development of theoretical foundations for the creation and implementation of a monitoring, diagnostic, treatment and wellness program for the protection of animals from epizootically significant infectious diseases» The system of anti-leukemic health measures using complex serological and hematological testing helps to reduce the number of RID+ animals and hematologically sick individuals for a 6-month period by 32.0% and 6.28%, respectively. The use of the polymerase chain reaction method contributes to the detection of an additional 18 to 39% of calves from 15 days of age, infected with the virus of bovine leukemia, in dysfunctional leukemia livestock enterprises. In a PCR study of cows, 29% to 54% of individuals infected with BLV can be detected. This indicates the need for consistent use of serological studies and the importance of molecular genetic tests for the detection of BLV infected calves.

scholarly journals Experience in the use of polymerase chain reaction in the diagnosis of bovine leukemia virus and its effectiveness at different stages of health activities

2019 ◽  
Vol 191 (12) ◽  
pp. 52-59
Author(s):  
Natalia Bezborodova ◽  
M. Petropavlovskiy ◽  
A. Romanova ◽  
A. Lysov ◽  
Veronika Kozhukhovskaya
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Immunological Tolerance ◽  
Adult Cattle ◽  
Serological Examination ◽  
Republic Of Bashkortostan ◽  
Serological Studies ◽  
Pcr Method ◽  
The Republic ◽  
High Concentrations

Abstract. Scientific novelty. The article presents the materials of long-term work on the use of PCR diagnostics together with serological methods (rid, ELISA) in the study of bovine leukemia virus of various physiological groups, as well as experimental animals (rabbits, small cattle).The aim of the study was to evaluate the PCR method in the diagnosis of bovine leukemia virus. Methods. Biomaterials (blood, colostrum) from cattle for PCR studies were obtained from 20 agricultural organizations of Tyumen, Chelyabinsk, Kurgan regions and the Republic of Bashkortostan. A total of 1,269 blood samples and 36 colostrum samples were examined by PCR. Laboratory methods (rid, ELISA, PCR) were used in the early diagnosis of leukemia virus in young cattle, as well as in the diagnosis of adult livestock. Results. The results obtained by PCR revealed carriers of the Lekota virus in calves aged 15 days to 1 month among young animals, which significantly reduced the duration of recreational activities (an average of 20 %). It was established that serological examination of newborn calves for the presence of antibodies to leukemia virus can give false results due to the influence of high concentrations of maternal antibodies and the development of immunological tolerance. PCR studies of biomaterial (samples 1023), taken from adult cattle (cows 3–4, 6 years), showed that 52 % of the samples were present leukemia virus, which was confirmed by serological studies. It is proved that up to 20 % of virus carriers from the number of rid-negative animals are detected during ELISA and PCR. Some types of BLV leukemia can not be detected by serological studies, but easily cope with PCR. The article deals with various types of PCR used in the diagnosis of leukemia, describes the data obtained from experiments on artificially infected animals with leukemia virus.

scholarly journals Early diagnosis of cervical cancer in the Republic of Bashkortostan: a pilot project

2020 ◽  
pp. 47-48
Author(s):  
А.Ф. Ахметгалеева ◽  
Р.И. Султанова ◽  
О.И. Машков ◽  
В.Л. Ахметова ◽  
М.В. Мельникова ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Early Diagnosis ◽  
Pilot Project ◽  
Cytological Examination ◽  
Republic Of Bashkortostan ◽  
The Republic ◽  
Positive Results

В рамках пилотного проекта сплошного организованного комбинированного скрининга рака шейки матки (РШМ) было обследовано 29084 жительниц г.Уфы в возрасте от 30 до 39 лет с использованием технологии ВПЧ Дайджен-тест (Digene-тест, QIAGEN) с последующим цитологическим исследованием ВПЧ позитивных женщин. При проведении лабораторного обследования положительный результат был выявлен в 2991 случае, что составило 9,8% всех обследованных. Из них у 274 пациентов в результате дообследования были выявлены предраковые изменения шейки матки, а у 19 - карциномы на I стадии. Данное исследование является первым опытом в России по ранней диагностики РШМ. 29,084 Ufa residents were examined for cervical cancer using HPV technology Digene-test (Digene-test, QIAGEN), as part of a pilot project of organized combined screening, followed by cytological examination of HPV positive women. Women were 30-39 years old. Positive results were detected in 2991 cases, which is 9.8% of all examined women. Precancerous changes in the cervix was revealed in 274 patients, and carcinomas - in 19 patients.

scholarly journals Development features of healthy anti-leukemic measures schemes considering the influence of the epizootic process on the example of the Republic of Bashkortostan

2021 ◽  
Vol 0 (14) ◽  
pp. 70-80
Author(s):  
Maksim Petropavlovskiy ◽  
A. Lysov ◽  
Al'bina Isaeva ◽  
Alisa Romanova
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Recovery Period ◽  
Farm Animals ◽  
Laboratory Methods ◽  
Operational Analysis ◽  
Republic Of Bashkortostan ◽  
Epizootic Process ◽  
The Republic ◽  
Academy Of Sciences

Abstract. The study of the features of the leukemic epizootic process in agricultural organizations of the Republic of Bashkortostan was carried out. The object of the study was cattle of different groups, including calves before drinking colostrum, heifers, springer heifers, pregnant and dairy cows. The purpose was to develop general schemes of anti-leukemic measures that guarantee a reduction in the recovery period for disadvantaged areas and a decrease in the level of infection of cattle with the leukemia virus. The work was carried out in the leukemia laboratory of the department for monitoring and predicting infectious animal diseases of the Ural Federal Agrarian Scientific Research Center of the Ural Branch of the Russian Academy of Sciences. Methods. A retrospective and operational analysis of data on the laboratory methods used for the diagnosis of bovine leukemia virus was carried out. Scientific novelty consists in obtaining new knowledge about the problems of diagnostics of the bovine leukemia virus among the populations of farm animals in the Republic of Bashkortostan. The features of the leukemic epizootic process have been studied: sources, transmission routes of the leukemia virus, level of infection and incidence of bovine leukemia virus in the surveyed farms in the regions of the Republic of Bashkortostan. Results. On the basis of the data obtained, general schemes of anti-leukemic measures have been developed, which guarantee a reduction in the recovery time of disadvantaged areas and a decrease in the level of infection of livestock with the leukemia virus.

scholarly journals Clinical genetic analysis of myotonia dystrophica in the republic of bashkortostan

1997 ◽  
Vol XXIX (1-2) ◽  
pp. 78-80
Author(s):  
R. V. Magzhanov ◽  
L. R. Akhmadeeva ◽  
Е. К. Khusnutdinova ◽  
V. F. Tunik ◽  
R. I. Fatkhlislamova ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Molecular Genetic ◽  
Data Bank ◽  
Genetic Characteristic ◽  
Clinical Genetic ◽  
Computer Data ◽  
Republic Of Bashkortostan ◽  
The Republic ◽  
Myotonia Dystrophica ◽  
First Time

In the Republic of Bashkortostan computer data bank on myotonia dystrophica (MD) was created where information on all diagnosed patients and members of their families was entered. For the first time in the republic qualitative molecular genetic RNA-diagnosis of MD started, with the help of which one phenotypically healthy mutation carrier is revealed. Clinical genetic characteristic of MD in Bashkortostan and main subpopulation of the republic is presented. Besides neuromuscular symptoms cerebral and extraneural symptoms are analyzed.

scholarly journals Communicable diseases common to man and animals – current state of the problem

2019 ◽  
pp. 36-45
Author(s):  
I. Subbotina ◽  
I. Andamov ◽  
B. Bakyev ◽  
I. Kuprijanov ◽  
Safar-zadeh Hamid Rafi k ogly
Keyword(s):  
Infectious Diseases ◽  
Key Words ◽  
Molecular Genetic ◽  
Zoonotic Diseases ◽  
Human Medicine ◽  
Current State ◽  
Comparative Aspect ◽  
The World ◽  
The Republic ◽  
Almost All

Оne of the signifi cant problems for the veterinary service and for human medicine workers around the world are infectious diseases (parasitic and infectious), common to humans and animals. In most countries, this group of diseases is called “zoonoses,” although in many countries and many researchers this term raises many questions and disagreements. In a number of countries, it is customary to strictly divide them into “zooanthroponoses” and “anthropozoonoses”. However, despite the diff erence in terminology, the importance of zoonotic diseases remains relevant for everyone. Of course, in countries with diff erent climatic, geographical, cultural and gastronomic characteristics, certain diseases will prevail, but their social and economic signifi cance, however, will be similar for everyone. In our work, we wanted in a comparative aspect to show the most signifi cant zoonoses and the dependence of their distribution on climatic, geographical, gastronomic, cultural and a number of other features of the countries. We have carried out work in such countries as the Republic of Belarus, Turkmenistan, Tajikistan and Azerbaijan. In our work, we identifi ed the most signifi cant zoonoses for the above countries and determined the main causes and factors contributing to the emergence and dissemination of these pathologies The studies were carried out using modern research methods, such as epizootological, virological, bacteriological, molecular genetic, statistical. As a result of the studies, it was found that a number of zoonotic diseases, such as rabies, pasteurellosis, tuberculosis, are quite widespread in almost all of these countries, while anthrax, brucellosis, echinococcosis, have a signifi cant diff erence in the intensity of spread, and the immediate causes of this diff erence are both climatic and geographical factors, and features of agriculture, and a number of others. Key words: zoonoses, infections, infestations, rabies, pasteurellosis, anthrax, brucellosis, tuberculosis, echinococcosis, cestodoses, trichinosis, ascariasis, cryptosporidiosis.

Epizootic situation on bovine leucose in Laksky municipal district of Dagestan

2020 ◽  
pp. 8-10
Author(s):  
Nurdin R. Budulov ◽  
◽  
Yusup S. Salikhov ◽  
Eldar M. Shikhragimov ◽  
Keyword(s):  
Direct Contact ◽  
Leukemia Virus ◽  
Well Being ◽  
Agricultural Enterprises ◽  
Municipal District ◽  
Serological Examination ◽  
Real Work ◽  
Target Program ◽  
The Republic ◽  
And Control

Results of the assessment of the epizootic situation on bovine leucose in Laksky district of the Republic of Dagestan for 2013-2019 are presented in the article. Authors established that the disease was recorded throughout the study period in various settlements and farms of the region. During monitoring studies in the municipal district, where the real work was carried out in 20052008, the level of infection of animals, on average, was 23,64%, the incidence rate - 2,88%. In 2013-2017 BLV infection was recorded almost every year in 35,29% of households. At the same time, the level of infection of animals annually ranged from 1,68 in 2013 to 6,74% in 2014, with a tendency to decrease to 2,68% in 2015, 1,92-2,53% in 2016-2017, making up, on average, 3,11±0.93% of the number examined for the analyzed five years. With the implementation of the republican target program in the region, cattle serological coverage increased from 4,04% in 2013-2017 to 84,27% in 2018-2019. At the same time, the incidence of new cases of seropositive animals decreased from 3,11 to 1,87%, respectively. In particular, the number of farms that were safe on BLV infection has increased, on average, from 8,8 to 60. In 2019 out of 92 agricultural enterprises, peasant farms, personal subsidiary farms, and individual entrepreneurial farms, 76 (82,61%) were prosperous on leukemia. In 16 (17,39%) households the infection of animals with leukemia virus was from 0,28 to 39,33%. Authors recommended timely removal of not only seropositive animals from the herd, but also regular serological examination of the herd to identify animals in direct contact with previously isolated virus carriers in bovine leukemia dysfunctional farms, which is the basis for recovery and control of epizootic well-being for leukemia.

scholarly journals Influence of seasonal changes in climatic conditions on losses at oil tanks filling (on example of the Republic of Bashkortostan)

2020 ◽  
Vol 175 ◽  
pp. 12021
Author(s):  
Alexey Elizaryev ◽  
Dmitry Tarakanov ◽  
Sergey Aksenov ◽  
Denis Tarakanov ◽  
Elena Elizareva ◽  
...  
Keyword(s):  
Environmental Pollution ◽  
Air Temperature ◽  
Atmospheric Pressure ◽  
Seasonal Changes ◽  
Climatic Conditions ◽  
Agricultural Enterprises ◽  
Oil Storage ◽  
Republic Of Bashkortostan ◽  
The Republic ◽  
Oil Tanks

Research objective: determining the effect of Influence of seasonal changes on losses at oil tanks filling (on example of the Republic of Bashkortostan). There are many types of oil losses, but most of all is filling loss. They are polluting the environment on the territory of agricultural and oil facilities. They can lead to fire, explosion at oil storage facilities, agricultural enterprises. Method of research: filling loss directly depend on the daily temperature and atmospheric pressure. The work has worked out the values of temperature and atmospheric pressure for the year. Based on the obtained values of air temperature and atmospheric pressure, the volume of big breathing was calculated. Results of the study: the greatest environmental pollution is observed in the summer. In General, there is a uniform dynamics of changes in big breathing throughout the year on the entire territory of the Republic ofBashkortostan.

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