A Rapid and Convenient Approach to Construct Porous Collagen Membranes via Bioskiving and Sonication-Feasible for Mineralization to Induce Bone Regeneration

Porous mineralized collagen membranes efficiently promote bone regeneration. To generate them, we need to fabricate collagen membranes that are porous. However, the current fabrication method is primarily based on a bottom-up strategy, with certain limitations, such as a long manufacturing process, collagen denaturation, and failure to control fibril orientation. Using a top-down approach, we explore a novel method for constructing porous collagen membranes via the combined application of bioskiving and sonication. Numerous collagen membranes with well-aligned fibril structures were rapidly fabricated by bioskiving and then sonicated at 30, 60, 90, and 120 W for 20 min. This treatment allowed us to study the effect of power intensity on the physicochemical traits of collagen membranes. Subsequently, the prepared collagen membranes were immersed in amorphous calcium phosphate to evaluate the feasibility of mineralization. Additionally, the bioactivities of the membranes were assessed using preosteoblast cells. Tuning the power intensity was shown to modulate fibril orientation, and the porous membrane without denatured collagen could be obtained by a 20-min sonication treatment at 90 W. The prepared collagen membrane could also be further mineralized to enhance osteogenesis. Overall, this study offers a rapid and convenient approach for fabricating porous collagen membranes via bioskiving and sonication.

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The Effects of Collagen Membrane Coated with PLGA on Bone Regeneration in Rat Calvarial Defects

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.357 ◽

2007 ◽

Vol 342-343 ◽

pp. 357-360

Author(s):

Kun Young Song ◽

Yoo Jung Um ◽

Ui Won Jung ◽

Yong Keun Lee ◽

Seong Ho Choi ◽

...

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Collagen Membrane ◽

Histomorphometric Analysis ◽

Local Bone ◽

Calvarial Defects ◽

Formation Behavior ◽

Surgical Implantation ◽

Collagen Membranes ◽

Rat Calvarial Defect

The purpose of this study was to evaluate the effects of collagen membrane coated with PLGA on bone regeneration in rat calvarial defect. Five groups of 10 animals each received either collagen membrane coated with 0.5%, 1%, 3% concentration of PLGA, collagen membrane only or surgical control. Each group of animals was healed into 2 healing periods of 2(5 animals) and 8(5 animals)weeks and histologic and histomorphometric analysis were done. The results of the following study revealed that surgical implantation of collagen membranes coated with PLGA enhanced local bone formation at both 2 and 8 weeks independent of different PLGA concentrations. In conclusion, collagen membrane coated with PLGA shows a significant bone formation behavior irrespective of their concentration.

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Evaluation of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Cross-Linked Collagen Membranes for Guided Bone Regeneration in Beagle Dogs

Materials ◽

10.3390/ma13204599 ◽

2020 ◽

Vol 13 (20) ◽

pp. 4599

Author(s):

Jong-Ju Ahn ◽

Hyung-Joon Kim ◽

Eun-Bin Bae ◽

Won-Tak Cho ◽

YunJeong Choi ◽

...

Keyword(s):

Tensile Strength ◽

Physical Properties ◽

Bone Regeneration ◽

Enzymatic Degradation ◽

Test Group ◽

Guided Bone Regeneration ◽

Control Group ◽

Collagen Membrane ◽

Micro Computed Tomography ◽

Collagen Membranes

The purpose of this study was to evaluate the bone regeneration efficacy of an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked collagen membrane for guided bone regeneration (GBR). A non-cross-linked collagen membrane (Control group), and an EDC-cross-linked collagen membrane (Test group) were used in this study. In vitro, mechanical, and degradation testing and cell studies were performed. In the animal study, 36 artificial bone defects were formed in the mandibles of six beagles. Implants were inserted at the time of bone grafting, and membranes were assigned randomly. Eight weeks later, animals were sacrificed, micro-computed tomography was performed, and hematoxylin-eosin stained specimens were prepared. Physical properties (tensile strength and enzymatic degradation rate) were better in the Test group than in the Control group. No inflammation or membrane collapse was observed in either group, and bone volumes (%) in defects around implants were similar in the two groups (p > 0.05). The results of new bone areas (%) analysis also showed similar values in the two groups (p > 0.05). Therefore, it can be concluded that cross-linking the collagen membranes with EDC is the method of enhancing the physical properties (tensile strength and enzymatic degradation) of the collagen membranes without risk of toxicity.

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Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects

International Journal of Implant Dentistry ◽

10.1186/s40729-021-00333-y ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Balazs Feher ◽

Karol Ali Apaza Alccayhuaman ◽

Franz Josef Strauss ◽

Jung-Seok Lee ◽

Stefan Tangl ◽

...

Keyword(s):

Bone Regeneration ◽

Soft Tissues ◽

Collagen Fibers ◽

Collagen Membrane ◽

Bony Defect ◽

Dense Layer ◽

Type I ◽

Trabecular Thickness ◽

Defect Area ◽

Collagen Membranes

Abstract Background Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes with their dense layer towards the soft tissue and their porous layer towards the bony defect area. However, evidence supporting this recommendation is lacking. This study aimed to determine whether the alignment of bilayer collagen membranes has an effect on bone regeneration. Methods In two groups of ten male Sprague-Dawley rats each, a 5-mm calvarial defect was created. Thereafter, the defect was randomly covered with a bilayer, resorbable, pure type I and III collagen membrane placed either regularly or upside-down (i.e., dense layer towards bone defect). After 4 weeks of healing, micro-computed tomography (μCT), histology, and histomorphometry of the inner cylindrical region of interest (4.5 mm in diameter) were performed to assess new bone formation and the consolidation of the collagen membrane in the defect area. Results Quantitative μCT showed similar bone volume (median 8.0 mm3, interquartile range 7.0–10.0 vs. 6.2 mm3, 4.3–9.4, p = 0.06) and trabecular thickness (0.21 mm, 0.19–0.23 vs. 0.18 mm, 0.17–0.20, p = 0.03) between upside-down and regular placement, both leading to an almost complete bony coverage. Histomorphometry showed comparable new bone areas between the upside-down and regularly placed membranes, 3.9 mm2 (2.7–5.4) vs. 3.8 mm2 (2.2–4.0, p = 0.31), respectively. Both treatment groups revealed the same regeneration patterns and spatial distribution of bone with and without collagen fibers, as well as residual collagen fibers. Conclusions Our data support the osteoconductive properties of collagen membranes and suggest that bone regeneration is facilitated regardless of membrane layer alignment.

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Guiding Bone Regeneration with a Novel Biodegradable Polymeric Membrane and Bioceramic Bone Grafts around Dental Implants

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.1417 ◽

2007 ◽

Vol 330-332 ◽

pp. 1417-1420

Author(s):

L. Li ◽

C.Y. Bao ◽

Guo Min Ou ◽

W.C. Chen ◽

X.J. Zhang ◽

...

Keyword(s):

Bone Graft ◽

Bone Regeneration ◽

Dental Implants ◽

Polymeric Membrane ◽

Collagen Membrane ◽

Graft Material ◽

The Novel ◽

Histomorphometric Analysis ◽

Mongrel Dog ◽

Collagen Membranes

The aim of this study is to evaluate treatment effects of novel GBR membranes (PDLLA membranes) applied to bony defects around dental implants on new bone regeneration alone or in combination with bioceramic bone graft (BIO-OSS®) on the mongrel dog model in comparison with collagen membrane (BIO-GIDE®) and therefore to assess the clinical values of the novel GBR membranes and bioceramic bone graft material in dental implantology. 6 implants were placed in bilateral mandibular of each mongrel dog after preparation of mid- crestal defects. The defects on one side were covered with PDLLA membranes alone or with bone graft, while the contra-lateral sides received collagen membranes alone or with bone graft. These animals were sacrificed at 2, 4, 8,12 weeks post the GBR-operation. And gross sample examination, standardized radiographs, 99mTc-MDP SPECT and histomorphometric analysis were taken. All examinations showed similar amounts of newly formed bone beneath both types of barriers. The PDLLA or collagen membrane with bone graft groups showed better results than the membrane used alone groups. The results indicated that PDLLA membranes especially with bioceramic bone graft presented an improved response in dealing with bony defects around implants.

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Proteomic Analysis of Porcine-Derived Collagen Membrane and Matrix

Materials ◽

10.3390/ma13225187 ◽

2020 ◽

Vol 13 (22) ◽

pp. 5187

Author(s):

Jung-Seok Lee ◽

Goran Mitulović ◽

Layla Panahipour ◽

Reinhard Gruber

Keyword(s):

Soft Tissue ◽

Bone Regeneration ◽

Proteomic Analysis ◽

High Performance ◽

Guided Bone Regeneration ◽

Soft Tissue Augmentation ◽

Collagen Membrane ◽

Tissue Augmentation ◽

Fibrillar Collagens ◽

Collagen Membranes

Collagen membranes and matrices being widely used in guided bone regeneration and soft tissue augmentation have characteristic properties based on their composition. The respective proteomic signatures have not been identified. Here, we performed a high-resolution shotgun proteomic analysis on two porcine collagen-based biomaterials designed for guided bone regeneration and soft tissue augmentation. Three lots each of a porcine-derived collagen membrane and a matrix derived from peritoneum and/or skin were digested and separated by nano-reverse-phase high-performance liquid chromatography. The peptides were subjected to mass spectrometric detection and analysis. A total of 37 proteins identified by two peptides were present in all collagen membranes and matrices, with 11 and 16 proteins being exclusively present in the membrane and matrix, respectively. The common extracellular matrix proteins include fibrillar collagens (COL1A1, COL1A2, COL2A1, COL3A1, COL5A1, COL5A2, COL5A3, COL11A2), non-fibrillar collagens (COL4A2, COL6A1, COL6A2, COL6A3, COL7A1, COL16A1, COL22A1), and leucine-rich repeat proteoglycans (DCN, LUM, BGN, PRELP, OGN). The structural proteins vimentin, actin-based microfilaments (ACTB), annexins (ANXA1, ANXA5), tubulins (TUBA1B, TUBB), and histones (H2A, H2B, H4) were also identified. Examples of membrane-only proteins are COL12A1 and COL14A1, and, of matrix only proteins, elastin (ELN). The proteomic signature thus revealed the similarities between but also some individual proteins of collagen membrane and matrix.

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The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes

Polymers ◽

10.3390/polym13173007 ◽

2021 ◽

Vol 13 (17) ◽

pp. 3007

Author(s):

Marta Vallecillo-Rivas ◽

Manuel Toledano-Osorio ◽

Cristina Vallecillo ◽

Manuel Toledano ◽

Raquel Osorio

Keyword(s):

Bone Regeneration ◽

Degradation Kinetics ◽

Hydrolytic Degradation ◽

Guided Bone Regeneration ◽

Collagen Membrane ◽

Bacterial Collagenase ◽

Degradation Tests ◽

Collagen Membranes ◽

Different Origins ◽

Porcine Pericardium

Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method.

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Fabrication and Characterization of Chitosan-Collagen Membrane from Barramundi (Lates Calcarifer) Scales for Guided Tissue Regeneration

European Journal of Dentistry ◽

10.1055/s-0039-1698610 ◽

2019 ◽

Vol 13 (03) ◽

pp. 370-375 ◽

Cited By ~ 1

Author(s):

Agus Susanto ◽

Mieke Hemiawati Satari ◽

Basril Abbas ◽

R. Setyo Adji Koesoemowidodo ◽

Arief Cahyanto

Keyword(s):

Mechanical Strength ◽

Tissue Regeneration ◽

Membrane Surface ◽

Porous Membrane ◽

Guided Tissue Regeneration ◽

Collagen Membrane ◽

Membrane Characterization ◽

Wet Condition ◽

Membrane Porosity ◽

Collagen Membranes

Abstract Objectives The aim of this study was to evaluate the synthesis, mechanical strength, and morphology of chitosan–collagen membranes from barramundi scales for guided tissue regeneration technique. Materials and Methods Collagen was extracted from barramundi scales by immersion in acetic acid. The resulting wet collagen was later dried. The membrane was fabricated by mixing chitosan with collagen from barramundi scales. Membrane characterization parameters were measured using Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and mechanical property. Results The FTIR spectrum showed the typical peak of the mixture of chitosan and collagen. The tensile strength and elongation at break of the membrane in dry condition were 0.28 MPa and 8.53%, respectively, while in the wet condition these were 0.12 MPa and 25.6%. The membrane porosity test result was 38.85%; SEM result showed a porous membrane surface with size varying around 16 to 100 µm. Conclusion The chitosan-collagen membrane from the barramundi scale showed the fibrous membrane surface that has ideal porous size as guided tissue regeneration membrane and the lower mechanical strength.

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Bone Regeneration Assessment of Polycaprolactone Membrane on Critical-Size Defects in Rat Calvaria

Membranes ◽

10.3390/membranes11020124 ◽

2021 ◽

Vol 11 (2) ◽

pp. 124

Author(s):

Ana Paula Farnezi Bassi ◽

Vinícius Ferreira Bizelli ◽

Tamires Mello Francatti ◽

Ana Carulina Rezende de Moares Ferreira ◽

Járede Carvalho Pereira ◽

...

Keyword(s):

Bone Regeneration ◽

Bone Healing ◽

Inflammatory Responses ◽

Blood Clot ◽

Healing Process ◽

Male Rats ◽

Control Group ◽

Collagen Membrane ◽

Large Area ◽

Rat Calvaria

Biomaterials for use in guided bone regeneration (GBR) are constantly being investigated and developed to improve clinical outcomes. The present study aimed to comparatively evaluate the biological performance of different membranes during the bone healing process of 8 mm critical defects in rat calvaria in order to assess their influence on the quality of the newly formed bone. Seventy-two adult male rats were divided into three experimental groups (n = 24) based on the membranes used: the CG—membrane-free control group (only blood clot, negative control), BG—porcine collagen membrane group (Bio-Guide®, positive control), and the PCL—polycaprolactone (enriched with 5% hydroxyapatite) membrane group (experimental group). Histological and histometric analyses were performed at 7, 15, 30, and 60 days postoperatively. The quantitative data were analyzed by two-way ANOVA and Tukey’s test (p < 0.05). At 7 and 15 days, the inflammatory responses in the BG and PCL groups were significantly different (p < 0.05). The PCL group, at 15 days, showed a large area of newly formed bone. At 30 and 60 days postoperatively, the PCL and BG groups exhibited similar bone healing, including some specimens showing complete closure of the critical defect (p = 0.799). Thus, the PCL membrane was biocompatible, and has the potential to help with GBR procedures.

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Dentin-Derived-Barrier Membrane in Guided Bone Regeneration: A Case Report

Materials ◽

10.3390/ma14092166 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2166

Author(s):

Jeong-Kui Ku ◽

In-Woong Um ◽

Mi-Kyoung Jun ◽

Il-hyung Kim

Keyword(s):

Case Report ◽

Bone Regeneration ◽

Guided Bone Regeneration ◽

Successful Outcome ◽

Collagen Membrane ◽

Type I ◽

Barrier Membrane ◽

Dentin Matrix ◽

Demineralized Dentin Matrix ◽

Demineralized Dentin

An autogenous, demineralized, dentin matrix is a well-known osteo-inductive bone substitute that is mostly composed of type I collagen and is widely used in implant dentistry. This single case report describes a successful outcome in guided bone regeneration and dental implantation with a novel human-derived collagen membrane. The authors fabricated a dentin-derived-barrier membrane from a block-type autogenous demineralized dentin matrix to overcome the mechanical instability of the collagen membrane. The dentin-derived-barrier acted as an osteo-inductive collagen membrane with mechanical and clot stabilities, and it replaced the osteo-genetic function of the periosteum. Further research involving large numbers of patients should be conducted to evaluate bone forming capacity in comparison with other collagen membranes.

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