Low Abundance Fusobacterium Nucleatum Supports Early Pregnancy Development – An In Vitro Study

Pregnancy success depends greatly on a balanced immune homeostasis. The detection of bacterial components in the upper reproductive tract in non-pregnant and pregnant women raised questions on its possible beneficial role in reproductive health. The local conditions that allow the presence of bacteria to harmonize with the establishment of pregnancy are still unknown. Among the described bacterial species in endometrial and placental samples, Fusobacterium nucleatum was found. It has been observed that F. nucleatum can induce tumorigenesis in colon carcinoma, a process that shares several features with embryo implantation. We propose that low concentrations of F. nucleatum may improve trophoblast function without exerting destructive responses. Inactivated F. nucleatum and E. coli were incubated with the trophoblastic cell lines HTR8/SVneo, BeWo, and JEG-3. Viability, proliferation, migratory capacity, invasiveness and the secretion of chemokines, other cytokines and matrix metalloproteinases were assessed. The presence of F. nucleatum significantly induced HTR8/SVneo invasion, accompanied by the secretion of soluble mediators (CXCL1, IL-6 and IL-8) and metalloproteinases (MMP-2 and MMP-9). However, as concentrations of F. nucleatum increased, these did not improve invasiveness, hindered migration, reduced cell viability and induced alterations in the cell cycle. Part of the F. nucleatum effects on cytokine release were reverted with the addition of a TLR4 blocking antibody. Other effects correlated with the level of expression of E-cadherin on the different cell lines tested. Low amounts of F. nucleatum promote invasion of HTR8/SVneo cells and induce the secretion of important mediators for pregnancy establishment. Some effects were independent of LPS and correlated with the expression of E-cadherin on trophoblasts.

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Effect of black seeds (Nigella sativa) volatile oil on the cervical cancer: In vitro study, on Hela cell lines

Advancement in Medicinal Plant Research ◽

10.30918/ampr.74.19.021 ◽

2019 ◽

Vol 7 (4) ◽

pp. 91-96

Author(s):

Isra'a Al-sobhi ◽

◽

Rawan Al-Ghabban ◽

Soad Shaker Ali ◽

Jehan Al-Amri ◽

...

Keyword(s):

Cervical Cancer ◽

Hela Cell ◽

Cell Lines ◽

Nigella Sativa ◽

In Vitro Study ◽

Volatile Oil ◽

Vitro Study ◽

Hela Cell Lines ◽

Black Seeds

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The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study

Molecules ◽

10.3390/molecules26133886 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3886

Author(s):

Stefania Sut ◽

Irene Ferrarese ◽

Maria Giovanna Lupo ◽

Nicola De Zordi ◽

Elisa Tripicchio ◽

...

Keyword(s):

Mass Spectrometry ◽

Cell Lines ◽

Hepatoma Cell ◽

Density Lipoprotein ◽

In Vitro Study ◽

Volatile Constituent ◽

Dependent Manner ◽

Human Hepatoma Cell ◽

Concentration Dependent Manner

In the present study the ability of supercritical carbon dioxide (SCO2) extracts of M. longifolia L. leaves to modulate low-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) expression was evaluated in cultured human hepatoma cell lines Huh7 and HepG2. Two SCO2 extracts, one oil (ML-SCO2) and a semisolid (MW-SCO2), were subjected to detailed chemical characterization by mono- and bidimensional nuclear magnetic resonance (1D, 2D-NMR), gas chromatography coupled with mass spectrometry (GC-MS) and liquid chromatography coupled with mass spectrometry (LC-MS). Chemical analysis revealed significant amounts of fatty acids, phytosterols and terpenoids. ML-SCO2 was able to induce LDLR expression at a dose of 60 µg/mL in HuH7 and HepG2 cell lines. Furthermore, ML-SCO2 reduced PCSK9 secretion in a concentration-dependent manner in both cell lines. Piperitone oxide, the most abundant compound of the volatile constituent of ML-SCO2 (27% w/w), was isolated and tested for the same targets, showing a very effective reduction of PCSK9 expression. The overall results revealed the opportunity to obtain a new nutraceutical ingredient with a high amount of phytosterols and terpenoids using the SCO2 extraction of M. longifolia L., a very well-known botanical species used as food. Furthermore, for the first time we report the high activity of piperitone oxide in the reduction of PCSK9 expression.

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In vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms

Canadian Journal of Microbiology ◽

10.1139/w08-032 ◽

2008 ◽

Vol 54 (6) ◽

pp. 501-508 ◽

Cited By ~ 17

Author(s):

Karina Cogo ◽

Michelle Franz Montan ◽

Cristiane de Cássia Bergamaschi ◽

Eduardo D. Andrade ◽

Pedro Luiz Rosalen ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Culture Media ◽

Bacterial Species ◽

In Vitro Study ◽

Bacterial Strains ◽

Planktonic Cells ◽

Susceptibility Tests

The aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. It also evaluated the ability of these bacteria to metabolize those substances. Single-species biofilms of Streptococcus gordonii , Porphyromonas gingivalis , or Fusobacterium nucleatum and dual-species biofilms of S. gordonii – F. nucleatum and F. nucleatum – P. gingivalis were grown on hydroxyapatite discs. Seven species were studied as planktonic cells, including Streptococcus oralis , Streptococcus mitis , Propionibacterium acnes , Actinomyces naeslundii , and the species mentioned above. The viability of planktonic cells and biofilms was analyzed by susceptibility tests and time-kill assays, respectively, against different concentrations of nicotine, cotinine, and caffeine. High-performance liquid chromatography was performed to quantify nicotine, cotinine, and caffeine concentrations in the culture media after the assays. Susceptibility tests and viability assays showed that nicotine, cotinine, and caffeine cannot reduce or stimulate bacterial growth. High-performance liquid chromatography results showed that nicotine, cotinine, and caffeine concentrations were not altered after bacteria exposure. These findings indicate that nicotine, cotinine, and caffeine, in the concentrations used, cannot affect significantly the growth of these oral bacterial strains. Moreover, these species do not seem to metabolize these substances.

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Synergistic Antiproliferative Effect in Combination Therapy of Δ12-Prostaglandin J2 and Hyperthermia-An in vitro Study with Human Esophageal Cancer Cell Lines

The Japanese Journal of Gastroenterological Surgery ◽

10.5833/jjgs.28.749 ◽

1995 ◽

Vol 28 (3) ◽

pp. 749-749

Author(s):

Tomohiro Saito ◽

Yoshinobu Yokoyama ◽

Toru Yunoki ◽

Mitsukazu Saito ◽

Yoshiaki Karaki ◽

...

Keyword(s):

Esophageal Cancer ◽

Combination Therapy ◽

Cell Lines ◽

Cancer Cell ◽

In Vitro Study ◽

Cancer Cell Lines ◽

Antiproliferative Effect ◽

Esophageal Cancer Cell ◽

Human Esophageal Cancer

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Anti-bacterial activity of three essential oils - An in vitro study

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i2.380 ◽

2019 ◽

Vol 10 (2) ◽

pp. 1049-1053 ◽

Cited By ~ 1

Author(s):

Geetha RV ◽

John Rozar Raj B ◽

Lakshmi Thangavelu

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Streptococcus Mutans ◽

Bacterial Species ◽

In Vitro Study ◽

Oral Bacteria ◽

Zone Of Inhibition ◽

Thyme Oil ◽

Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

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Erratum to: Bactericidal effect of visible light in the presence of erythrosine on Porphyromonas gingivalis and Fusobacterium nucleatum compared with diode laser, an in vitro study

LASER THERAPY ◽

10.5978/islsm.14-er-01 ◽

2014 ◽

Vol 23 (4) ◽

pp. 263-271 ◽

Cited By ~ 1

Author(s):

Ghanbari Habiboallah ◽

Zakeri Mahdi ◽

Naderi Nasab Mahbobeh ◽

Zareian Jahromi Mina ◽

Faghihi Sina ◽

...

Keyword(s):

Visible Light ◽

Diode Laser ◽

Porphyromonas Gingivalis ◽

In Vitro Study ◽

Fusobacterium Nucleatum ◽

Bactericidal Effect ◽

Vitro Study

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Ellagic Acid Cytotoxic and Metalloproteinases Inhibitory Effects Against Oral Squamous Cell Carcinoma: An in Vitro Study

10.21203/rs.3.rs-879044/v1 ◽

2021 ◽

Author(s):

Patricia Maria Wiziack Zago ◽

Luiza Rodrigues Hellmeister ◽

Lucas Novaes Teixeira ◽

Rui Barbosa de Brito Junior ◽

Elizabeth Ferreira Martinez

Keyword(s):

Oral Cancer ◽

Cell Lines ◽

Ellagic Acid ◽

Normal Cell ◽

In Vitro Study ◽

Significance Level ◽

Viability Analysis ◽

Anticancer Properties

Abstract ObjectivesThis study aimed to evaluate the in vitro antitumoral potential of different concentrations of EA against two OSCC cell lines with distinct tissue invasiveness profiles. Material and methodsNormal keratinocytes (NOK) and OSCC´s cells CAL-27 and SCC-9 were treated with concentrations of EA varying from 5 to 662 µM during 24, 48 or 72h. After each time of treatment, cells were submitted to viability analysis using MTT and the secretion of metalloproteinases (MMP-2 and MMP-9) and tissue metalloproteinases inhibitors (TIMP-1 and TIMP-2) were performed by Enzyme-Linked Immunoassay (ELISA). Data were submitted to ANOVA, followed by Bonferroni´s test, considering 5% as significance level. ResultsEA was cytotoxic to OSCC cells in all exposure times, rarely affecting normal cell viability, except for concentrations higher than 82 µM and after 72h treatment. For OSCC cells, EA decreased MMPs and increased TIMPs´s expression without effect on those enzymes for normal cell lines during all times of exposure. ConclusionEA is a promising therapeutic adjuvant to treat oral cancer, however, further in vivo studies are required to clinically validate its potential. Clinical RelevanceThe in vitro anticancer properties showed by Ellagic acid, a phenolic compound that could easily be accessed by oral cancer patients, provides data to base future clinical studies intended to develop a safe topical oral anticancer product.

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Irinotecan Resistance – In Search of New Markers in CRC.

10.21203/rs.3.rs-1088696/v1 ◽

2021 ◽

Author(s):

Jakub Kryczka ◽

Joanna Boncela

Keyword(s):

Drug Resistance ◽

Cell Lines ◽

Expression Profiles ◽

Resistance Mechanism ◽

Interaction Network ◽

In Vitro Study ◽

Cancer Drug ◽

New Genes

Abstract Colorectal cancer (CRC) is one of the most prominent causes of cancer death worldwide. Chemotherapeutic regimens consisting of different drugs combinations such as 5-fluorouracil, and oxaliplatin (FOLFOX) or irinotecan (FOLFIRI) have been proven successful in the treatment of CRC. However, chemotherapy often leads to the acquisition of cancer drug resistance followed by metastasis and in the aftermath therapeutic failure. The molecular mechanism responsible for drug resistance is still unclear. The systemic search for new biomarkers of this phenomenon may identify new genes and pathways. To understand the drug resistance mechanism in CRC, the in vitro study based on the molecular analysis of drug-sensitive cells lines vs drug-resistant cells lines has been used. In our study to bridge the gap between in vitro and in vivo study, we compared the expression profiles of cell lines and patient samples from the publicly available database to select the new candidate genes for irinotecan resistance. Using The Gene Expression Omnibus (GEO) database of CRC cell lines (HT29, HTC116, LoVo, and their respective irinotecan-resistant variants) and patient samples (GSE42387, GSE62080, and GSE18105) we compared the changes in the mRNA expression profile of the main genes involved in irinotecan body’s processing, such as transport out of the cells and metabolism. Furthermore, using a protein-protein interaction network of differently expressed genes between FOLFIRI resistant and sensitive CRC patients, we have selected top networking proteins (upregulated: NDUFA2, SDHD, LSM5, DCAF4, and COX10, downregulated: RBM8A, TIMP1, QKI, TGOLN2, and PTGS2). Our analysis provided several potential irinotecan resistance markers, previously not described as such.

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