scholarly journals Assessment of Expression of SOCS Genes in Acquired Immune-Mediated Polyneuropathies

2021 ◽  
Vol 12 ◽  
Author(s):  
Mohammad Taheri ◽  
Somayeh Sangseifid ◽  
Pariya Shahani ◽  
Mohammad Mahdi Eftekharian ◽  
Shahram Arsang-Jang ◽  
...  
Keyword(s):  
Specific Pattern ◽  
Bayesian Regression ◽  
Cytokine Signaling ◽  
Control Group ◽  
Operating Characteristics ◽  
Mean Values ◽  
Immune Mediated ◽  
Male Patients ◽  
Underlying Mechanisms ◽  
And Control

Acquired immune-mediated polyneuropathies are classified to some subtypes among them are acute and chronic inflammatory demyelinating polyradiculoneuropathies (AIDP and CIDP). These two conditions share some common signs and underlying mechanisms. Based on the roles of Suppressor of cytokine signaling (SOCS) genes in the modulation of immune system reactions, these genes might be involved in the pathogenesis of these conditions. We evaluated expression of SOCS1-3 and SOCS5 genes in the leukocytes of 32 cases of CIDP, 19 cases of AIDP and 40 age- and sex-matched controls using real time PCR method. The Bayesian regression model was used to estimate differences in mean values of genes expressions between cases and control group. Expression levels of SOCS1 and SOCS2 were significantly lower in male patients compared with controls. This sex-specific pattern was also observed for SOCS3 down-regulation. Based on the area under curve values in Receiver Operating Characteristics (ROC) curve, diagnostic powers of SOCS1, SOCS2, SOCS3 and SOCS5 genes in the mentioned disorder were 0.61, 0.73, 0.68 and 0.58, respectively. Expression of none of genes was correlated with age of enrolled cases. The current study shows evidences for participation of SOCS genes in the pathophysiology of acquired immune-mediated polyneuropathies.

scholarly journals In vitro activity of zinc oxide-eugenol and glass ionomer cements on Candida albicans

2005 ◽  
Vol 19 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Anna Carolina Aguiar Cassanho ◽  
Aletéia Massula Fernandes ◽  
Luciane Dias de Oliveira ◽  
Claudio Antonio Talge Carvalho ◽  
Antonio Olavo Cardoso Jorge ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Candida Albicans ◽  
Control Group ◽  
Glass Ionomer ◽  
Experimental Conditions ◽  
Mean Values ◽  
Zinc Oxide Eugenol ◽  
Colony Forming Units ◽  
And Control

The aim of this study was to evaluate in vitro the antimicrobial activity of glass ionomer (GIC) and zinc oxide-eugenol (ZOE) cements against Candida albicans. Standardized GIC and ZOE specimens were maintained in contact with C. albicans suspension (1 <FONT FACE=Symbol>´</FONT> 10(6) cells/ml) at 37°C for 24 h, 48 h or 7 days. A control group without any testing cement was included. After the incubation period, aliquots of 0.1 ml were plated on Sabouraud's agar, and then the number of colonies was counted. The results were expressed as values of logarithms of colony-forming units per milliliter (log CFU/mL) and were analyzed statistically by Kruskal-Wallis ANOVA. After 48 h of incubation, the ZOE group presented no growth of C. albicans. GIC and control groups presented similar mean values at all tested periods. According to the results obtained, it could be concluded that, under the experimental conditions, ZOE cement was more effective in vitro against C. albicans than GIC.

scholarly journals Clinical Impact of Estradiol/Testosterone Ratio in Patients with Acute Ischemic Stroke

2020 ◽  
Author(s):  
Jung-Won Choi ◽  
In Woo Ryoo ◽  
Jun Yeong Hong ◽  
Kyung-Yul Lee ◽  
Hyo Suk Nam ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Sex Hormones ◽  
Control Group ◽  
Control Groups ◽  
Sexual Hormones ◽  
Stroke Group ◽  
Male Patients ◽  
And Control

Abstract Background: Sex hormones may be associated with a higher incidence of ischemic stroke or stroke-related events. In observational studies, lower testosterone concentrations are associated with infirmity, vascular disease, and adverse cardiovascular risk factors. Currently, female sexual hormones are considered neuroprotective agents. The purpose of this study was to assess the role of sex hormones and the ratio of estradiol/testosterone (E/T) in patients with acute ischemic stroke (AIS).Methods: Between January 2011 and December 2016, 146 male patients with AIS and 152 age- and sex-matched control subjects were included in this study. Sex hormones, including estradiol, progesterone, and testosterone, were evaluated in the AIS patient and control groups. We analyzed the clinical and physiological levels of sex hormones and hormone ratios in these patients.Results: The E/T ratio was significantly elevated among patients in the stroke group compared to those in the control group (P = 0.001). Categorization of data into tertiles revealed that patients with the highest E/T ratio were more likely to have AIS [odds ratio (OR) 3.084; 95% Confidence interval (CI): 1.616-5.886; P < 0.001) compared with those in the first tertile. The E/T ratio was also an independent unfavorable outcome predictor with an adjusted OR of 1.167 (95% CI: 1.053-1.294; P = 0.003).Conclusions: These findings support the hypothesis that increased estradiol and reduced testosterone levels are associated with AIS in men.

Evaluation of Parasitological Homeopathic Complex in the Control of Gastrointestinal Nematodes in Peripartum Sheep

Homeopathy ◽  
2019 ◽  
Vol 108 (04) ◽  
pp. 248-255
Author(s):  
Poliana Araújo Pacheco ◽  
Michael Nunes Marconato ◽  
Letícia Liepkaln dos Reis ◽  
Luciana Morita Katiki ◽  
Cláudia Cristina Paro de Paz ◽  
...  
Keyword(s):  
Body Weight ◽  
Anthelmintic Resistance ◽  
White Cell Count ◽  
Gastrointestinal Nematodes ◽  
Control Group ◽  
Mean Values ◽  
Fecal Egg Count ◽  
Peripartum Period ◽  
And Control ◽  
Increased Susceptibility

Background The appearance of anthelmintic resistance has made it difficult to control verminosis in sheep, leading to increased research to find alternative nematode control. The use of homeopathy in veterinary medicine has been studied as an alternative for the treatment and control of some diseases. In this study, ewes received an anti-parasitic homeopathic complex medicine during the critical peripartum period of increased susceptibility to nematodes. Methods Three randomized groups containing 16 animals each were assigned as follows: ‘H10’ received 10 g homeopathic complex added to concentrated food per day; ‘H20’ received 20 g homeopathic complex added to concentrated food per day; ‘C’ (control group) did not receive a homeopathic complex. Animals were tested to evaluate the effect of homeopathy on several health parameters during a period of 110 days. Results The parasite that prevailed in the copro-cultures of both treatments throughout the experiment was Haemonchus contortus (78.26%). Packed cell volume averages did not present statistically significant differences between the treatments (24.5, 24.4 and 23.9% to C, H10 and H20, respectively; p < 0.05). For total white cell count, lower mean values ( ±  standard error of mean) were observed for the H20 treatment (5,490.9 ± 0.02/µL; p < 0.05), whereas the H10 (5,919.4 ± 0.02/µL) and control (6,098.5 ± 0.02/µL) presented higher and similar averages (p > 0.05). The values for erythrocytes, monocytes, and lymphocytes did not show differences between treatments (p > 0.05). Body weight was greater in the H10-treated animals compared with control. For the fecal egg count (FEC) of Trichostrongylidae and Strongyloides spp., respectively, the averages of the H20 treatment (1,523.0 and 30.6) were not different from control (1,616.0 and 31.6) and H10 (1,038.0 and 27.6); for Trichostrongylidae, however, H10 presented a lower FEC than the control (p = 0.02). For Cooperia, H10- and H20-treated animals showed FEC reductions of 97% and 98%, respectively. Conclusions H20 treatment in peripartum sheep resulted in greater body weight and lower leukocyte count. H10 was associated with lower FEC for Trichostrongylidae. Both H10 and H20 justify a label of ‘effective anthelmintic’ for Cooperia.

scholarly journals Foetal Haemoglobin, Erythrocytes Containing Foetal Haemoglobin, and Hematological Features in Congolese Patients with Sickle Cell Anaemia

Anemia ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
L. Tshilolo ◽  
V. Summa ◽  
C. Gregorj ◽  
C. Kinsiama ◽  
J. A. Bazeboso ◽  
...  
Keyword(s):  
Sickle Cell ◽  
Mean Value ◽  
Hplc Method ◽  
Control Group ◽  
Foetal Haemoglobin ◽  
Heterogeneous Distribution ◽  
Mean Values ◽  
F Cells ◽  
Low Levels ◽  
And Control

High HbF levels and F cells are correlated with reduced morbidity and mortality in sickle cell disease (SCD). This paper was designed to determine the HbF and F cells levels in Congolese sickle cell anemia (SCA) patients in order to determine their impact on the expression of SCD.Population and Method. HbF levels were measured in 89 SCA patients (mean age 11.4 yrs) using a standard HPLC method. F cell quantitation was done in a second group of SCA patients (, mean age 8.9 yrs) and compared with a control group (, mean age 5 yrs). F cells were quantified by a cytofluorometric system (MoAb-HbF—FITC; cut off at 0.5%).Results. The mean value of HbF was 7.2%  5.0 with heterogeneous distribution, most patients (76%) having HbF < 8%. Mean values of F-cells in SCA patients and control group were 5.4%  7.6 (median: 2.19% ; range 0,0–30,3%) and 0.5%  1.6 (median 0.0, range 0–5.18), respectively. SCA patients with F cells >4.5% developed less painful crisis and had higher percentage of reticulocytes.Conclusion. Congolese SCA patients displayed low levels of HbF and F-cells that contribute to the severity of SCD.

scholarly journals Measurement of glycosaminoglycans in canine synovial fluid and its correlation with the cause of secondary osteoarthritis, age and body weight

2012 ◽  
Vol 81 (3) ◽  
pp. 287-294 ◽  
Author(s):  
Radka Andrysíková ◽  
Hana Kudláčková ◽  
Miroslav Toman ◽  
Alois Nečas
Keyword(s):  
Body Weight ◽  
Synovial Fluid ◽  
Mean Value ◽  
Control Group ◽  
Joint Cartilage ◽  
Mean Values ◽  
Secondary Osteoarthritis ◽  
Age Related ◽  
Normal Body Weight ◽  
And Control

Glycosaminoglycans are natural components of healthy joint cartilage and they also appear in healthy synovial fluid. An increased amount of glycosaminoglycans in synovial fluid is believed to be a marker of secondary osteoarthritis, regardless of its primary cause. The aim of our study was to define the relationship between glycosaminoglycans in the synovial fluid and joint disorders, age, and body weight. The samples of synovial fluid were obtained from dogs suffering from secondary secondary osteoarthritis (n = 35) and from control dogs (n = 18); control dogs had normal body weight. The results were compared among joints of dogs with secondary osteoarthritis divided into groups according to the criteria mentioned above and control dogs. Glycosaminoglycan concentrations in synovial fluid were measured using dimethylmethylene blue assay. The lowest mean value of glycosaminoglycans in synovial fluid was measured in the control group. Significantly higher glycosaminoglycan content (P < 0.05) was found in synovial fluid isolated from obese dogs compared to control dogs. Furthermore, we observed an age-related trend, in which the highest mean values were reached either in old dogs or pups. Despite the absence of significant differences in glycosaminoglycan values among dogs suffering from various types of secondary secondary osteoarthritis, the highest mean values were measured in fragmented coronoid processus group. Our data suggest that abnormally increased body weight has an impact on glycosaminoglycan concentration in synovial fluid which may imply faster degradation and turnover of joint cartilage. Such observation has not yet been published in veterinary medicine.

scholarly journals Role of Vitamin D in Near Sightedness

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Yasir Iqbal Malik ◽  
Aqsa Malik ◽  
Rabbia Shabbir ◽  
Atteaya Zaman ◽  
Sohail Zia ◽  
...  
Keyword(s):  
Vitamin D ◽  
Sampling Technique ◽  
Control Group ◽  
Mean Values ◽  
Group I ◽  
Vitamin D Levels ◽  
Significant Difference ◽  
Azad Kashmir ◽  
And Control

Purpose: To determine the levels of Vitamin D in children with myopia and to compare them with age matched controls. Study Design:  Case control study. Place and Duration of Study:  The study was conducted in Naseer Memorial Hospital, Dadhyal Azad Kashmir from March 2016 to March 2017. Material and Methods:  Two hundred patients were selected using convenient sampling technique and were divided two groups (group I Myopic and group II control). Myopia was labeled if after subjective refraction a Spherical Equivalent (SE) of −0.50 diopters (D) or more was found. Vitamin D levels were measured by radioimmunoassay technique with Diasorin SR® kit following the user’s manual. Vitamin D levels less than 20 ng/ml were considered Vitamin D deficient following the standards of American academy of pediatrics. The collected data was entered in the statistical package for social sciences (SPSS) version 21 for analysis. Independent t–test was used to determine the significant difference of means between controls and patients. P-values less than 0.05 were considered significant. Results:  Mean age of controls and myopes were 10.65 ± 3.9 and 10.20 ± 2.5 years respectively. Vitamin D levels in myopic children were found to be 14.95 ± 3.75 ng/ml and there was no significant difference in mean values of Vitamin D levels in myopic and control group. Conclusion:  We found no difference in Vitamin D levels of myopic and non myopic children and concluded that Vitamin D has no role in development or progression of myopia.

scholarly journals Choroidal Changes of Long-Term Type 1 Diabetic Patients without Retinopathy

Diagnostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 235 ◽  
Author(s):  
Elvira Orduna-Hospital ◽  
Lorena Perdices ◽  
Ana Sanchez-Cano ◽  
Javier Acha ◽  
Nicolás Cuenca ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Control Group ◽  
Diabetic Patients ◽  
Swept Source ◽  
Disease Evolution ◽  
Mean Values ◽  
Group 2 ◽  
And Control ◽  
Sd Oct

The aim of the study is to assess choroidal thickness (CT) and choroidal volume (CV) in 90 type 1 diabetes mellitus (DM1) patients with no diabetic retinopathy (DR) and 60 control eyes using spectral domain optical coherence tomography (SD-OCT) and swept source (SS)-OCT in the areas of the Early Treatment Diabetic Retinopathy Study (ETDRS). Mean ages were 42.93 ± 13.62 and 41.52 ± 13.05 years in the diabetic and control groups, respectively. Significant differences were obtained between both groups with Spectralis SD-OCT in all ETDRS areas and in the total CV, excluding the temporal perifoveal one. With Triton SS-OCT, statistically significant differences were obtained in the subfoveal CT and in the vertical areas. CT showed the same tendency with both OCTs, with greater CT and CV in the DM1 group than the mean values of the control group. To assess the influence of DM1 evolution in the CT modifications, DM1 patients were divided into Group 1, with less than 24 years of diagnosis, and Group 2, with ≥24 years of DM1 evolution. Using both OCTs, seven of the nine ETDRS areas and the CV had lower values in Group 2. CT and CV measured by OCT were higher in DM1 without DR. There is a choroidal thinning related to disease evolution in DM1. In patients with DM evolution greater than 24 years, the CT is statistically lower than in patients with less evolution of the disease.

scholarly journals Photodynamic Therapy Enhanced the Antitumor Effects of Berberine on HeLa Cells

2019 ◽  
Vol 17 (1) ◽  
pp. 413-421 ◽  
Author(s):  
Han-Qing Liu ◽  
Ya-Wen An ◽  
A-Zhen Hu ◽  
Ming-Hua Li ◽  
Guang-Hui Cui
Keyword(s):  
Photodynamic Therapy ◽  
Western Blot ◽  
Hela Cells ◽  
Mammalian Target Of Rapamycin ◽  
Control Group ◽  
Antitumor Effects ◽  
Underlying Mechanisms ◽  
And Control

AbstractIn this study we investigated the antineoplastic effects of Berberine (BBR)-mediated photodynamic therapy (PDT) on HeLa cells and its related mechanisms. The CCK-8 assay and flow cytometry were used to evaluate the proliferation and apoptosis of cells respectively. In addition, changes in protein expression levels were assessed using western blot. BBR at dose of 10 mg/kg was injected intraperitoneally to mice with tumors and PDT treatments were performed 24 hours later. In vivo imaging systems were used to evaluate the fluorescence of BBR. In vitro, PDT significantly enhanced the effects of BBR on inducing cell apoptosis and inhibiting proliferation. The in vivo results showed that the fluorescence intensity in the PDT group was decreased compared with that in the BBR group. Tumor weights and tumor size in the PDT group were less than those in the control group; however, when BBR was applied without PDT, no significant differences were observed between the BBR and control group. The results of western blot showed that PDT enhanced the inhibitory effects of BBR on the mammalian target of rapamycin (mTOR) signaling pathway, that may partly explain the potential underlying mechanisms.

scholarly journals Evaluation of Craniofacial Morphology of Children with Dental Fluorosis in Early Permanent Dentition Period

2009 ◽  
Vol 03 (04) ◽  
pp. 304-313
Author(s):  
Alev Aksoy Dogan ◽  
Pinar Bolpaca
Keyword(s):  
Dental Fluorosis ◽  
Permanent Dentition ◽  
T Test ◽  
Craniofacial Morphology ◽  
Control Group ◽  
Test Analysis ◽  
Mean Values ◽  
Significant Difference ◽  
Angular Measurements ◽  
And Control

ABSTRACTObjectives: High intake of fluoride (>1.5 mg/L) for a prolonged period may lead to skeletal fluorosis as well as dental fluorosis. The aim of this study was to compare the craniofacial characteristics of children with dental fluorosis in early permanent dentition period to those without fluorosis.Methods: Two hundred and sixteen children in early permanent dentition (girls:121, boys:95) were included in the study. Study group was composed of 124 children with dental fluorosis who was born and grew up in Isparta (girls:75, boys:49) whereas control group of children (n=92: 46 girls and 46 boys) had no dental fluorosis. Dental fluorosis was classified using Thylstrup Fejerskov Fluorosis Index. Radiological evaluation was performed by cephalometric tracing using Björk analysis. Statistical evaluation in between study and control groups was done by Independent Samples T test and comparison with Björk’s standards was done by One Sample T test analysis. The association between two quantitative variables was evaluated with Pearson’s correlation coefficient (rho).Results: The mean dental fluorosis level was 4.6±1.8 for children with fluorosis. Systemic fluorosis affect girls no different than boys in the early permanent dentition period because none of the angular measurements show significant difference between boys and girls in the fluoridated group. Comparison of craniofacial angular values of boys with fluorosis show greater diversity compared to boys without fluorosis against Björk’s mean values for boys.Conclusions: Craniofacial morphology of children with fluorosis did not show great diversity than the ones without fluorosis in the early permanent dentition period. None of the angular measurements were significantly different between boys and girls in the fluoridated group which might imply that systemic fluorosis did not show gender difference in the early permanent dentition. (Eur J Dent 2009;3:304-313)

Vibration disease: erectile dysfunction as a comorbidity problem

2020 ◽  
pp. 36-43
Author(s):  
S. Babanov ◽  
N. Tatarovskay
Keyword(s):  
Erectile Dysfunction ◽  
Questionnaire Survey ◽  
Erectile Function ◽  
Control Group ◽  
Local Vibration ◽  
Vibration Disease ◽  
Male Patients ◽  
And Control ◽  
The Impact ◽  
Second Degree

The article presents data (based on a questionnaire survey) on the impact of vibration disease from the action of local and General vibration on the erectile function of men. The main changes in the parameters of erectile function (frequency, need, etc.) are described in the case of first and second-degree vibrational disease caused by local vibration, and in the case of first-and second-degree vibrational disease caused by General vibration based on data from a questionnaire survey of male patients and control group.

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