scholarly journals How Does Pikeperch Sander lucioperca Respond to Dietary Insect Meal Hermetia illucens? Investigation on Gut Microbiota, Histomorphology, and Antioxidant Biomarkers

2021 ◽  
Vol 8 ◽  
Author(s):  
Hung Quang Tran ◽  
Markéta Prokešová ◽  
Mahyar Zare ◽  
Tatyana Gebauer ◽  
Antonia Concetta Elia ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Crude Protein ◽  
Oxidative Enzyme ◽  
Glutathione S Transferase ◽  
Sander Lucioperca ◽  
Hermetia Illucens ◽  
Fishmeal Replacement ◽  
Predominant Bacterium ◽  
Low Levels ◽  
Liver Degeneration

Effects of feeding dietary defatted black soldier fly (Hermetia illucens) larvae meal (HI) on intestine microbiota, and on histomorphology, oxidative enzyme activities in liver and intestine of pikeperch (Sander lucioperca) were investigated. Four isoproteic (45% crude protein) and isolipidic (18% ether extract) diets were formulated to include 0% (CO), 9% (HI9), 18% (HI18) and 36% (HI36) of HI as replacement for fishmeal at 0, 25, 50, and 100%, respectively, and were fed to triplicate groups of juvenile pikeperch (initial body weight, 68.7 ± 7.1 g) for 84 days. No adverse effects were detected on the intestine of pikeperch fed diet groups, in terms of histomorphology (P > 0.05), while fish fed free or low levels of HI (≤ 9% in diet) showed significant liver degeneration (P < 0.05). Dietary HI significantly affected the oxidative enzyme activities of catalase and glutathione peroxidase in the liver, and glutathione S-transferase in the intestine (P < 0.05), while activity of superoxide dismutase in both liver and intestine was HI-dose independent (P > 0.05). Feeding HI-containing diets positively modulated the richness and diversity of intestinal microbiota, especially for HI18 group (P < 0.05). Inclusion HI up to 18% (50% fishmeal replacement) in pikeperch diets increased abundance of Clostridium, Oceanobacillus, Bacteroides, and Faecalibacterium genera, whereas the predominant bacterium, Cetobacterium was found in control and HI36 groups. This study reveals the potential of HI as an immune and health booster for juvenile pikeperch.

scholarly journals Substitution of Fishmeal with Black Soldier Fly Hermetia illucens Linnaeus, 1758 Larvae in Finfish Aquaculture – A Review

2021 ◽  
Vol 34 (2) ◽  
Author(s):  
MANIYANGAMAGE KASUN CHATHURANGA PRIYADARSHANA ◽  
◽  
CHAMINDA NIROSHAN WALPITA ◽  
MANOHARAN NAVEENAN ◽  
MANJULA PRIYANTHA SUMITH MAGAMAGE ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Crude Protein ◽  
Pupal Stage ◽  
High Energy ◽  
Protein Source ◽  
Fish Nutrition ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Fishmeal Replacement ◽  
Wide Range

The reliance of aquaculture feed industry on fishmeal has been challenged by ecological, economic, and ethical limitations leading fish nutritionists to look for viable alternatives, and conventional animal-based protein sources showed varying degrees of success in this regard. However, a non-conventional protein source, black soldier fly Hermetia illucens Linnaeus, 1758 larvae (BSFL), received due attention as a focal point in fish nutrition research. Though many studies were conducted on H. illucens as a potential protein source for fish, a comprehensive review is not available. Therefore, this article aims to review the existing literature on the use of BSFL as a fishmeal replacer in aquaculture. The nutrient composition of BSFL varied with the developmental stage and the composition of growing medium. High crude protein levels (40.4–56.2 %) and high crude fat levels (4.8–24.8 %) made BSFL suitable for high protein and high energy diet. Early prepupa was the best stage for harvesting, considering its high crude protein content and dry matter yield and less chitin content. Up to 100 % inclusion levels of BSFL meal were tested in a wide range of fish species. Most studies revealed that up to 50 % of fishmeal replacement would be possible without negative effects on the fish. Notable adverse effects were found beyond 50 % of fishmeal replacement, mainly due to high chitin levels in the pupal stage and high crude fat levels. Harvesting BSFL before the pupal stage and defattening made it possible to replace 100 % of fishmeal without adverse effects on fish.

scholarly journals Effect of Processed Beverage By-Product-Based Diets on Biological Parameters, Conversion Efficiency and Body Composition of Hermetia illucens (L) (Diptera: Stratiomyidae)

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 475
Author(s):  
Vassilios Sideris ◽  
Maria Georgiadou ◽  
Georgios Papadoulis ◽  
Konstantinos Mountzouris ◽  
Antonios Tsagkarakis
Keyword(s):  
Body Composition ◽  
Crude Protein ◽  
Larval Density ◽  
Mass Reduction ◽  
Spent Coffee Grounds ◽  
Hermetia Illucens ◽  
Protein Levels ◽  
Spent Grains ◽  
Coffee Grounds ◽  
By Products

The effect of spent coffee grounds (SCG), brewer’s spent grains (BSG) and their mixtures with the addition of brewer’s yeast (BY) were tested in two rearing densities of the Black Soldier Fly, Hermetia illucens (L.). Different treatments were investigated on larval development, survival, yield, protein conversion (PrCR) and bioconversion rate (BCR), substrate mass reduction and body composition of the insect. BSF larvae were able to develop sufficiently in all diets, except on sole SCG. The addition of BY enhanced the performance properties of diets, especially in the case of SCG, where larvae underperformed. Substrate mass reduction, PrCR and BCR were affected only by feed and exhibited higher values on reference feed, followed by BSG and SCG+BSG enriched with BY. Density did not have a significant effect on various larval nutrients, except for fat, which was higher on larvae fed enriched feeds with BY and in the 300 larval density. The interaction between feed and density strongly affected the nitrogen and protein levels, larval yield and ash. Generally, diets which contained SCG exhibited high larval crude protein levels. Our results illustrate that low value beverage by-products can be successfully utilized as constituents of a successful BSF diet.

scholarly journals Fishmeal Replacement with Hermetia illucens Meal in Aquafeeds: Effects on Zebrafish Growth Performances, Intestinal Morphometry, and Enzymology

Fishes ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 28
Author(s):  
Baldassare Fronte ◽  
Rosario Licitra ◽  
Carlo Bibbiani ◽  
Lucia Casini ◽  
Mahanama De Zoysa ◽  
...  
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Control Diet ◽  
Low Carbon ◽  
Replacement Rate ◽  
Hermetia Illucens ◽  
Fishmeal Replacement ◽  
Fish Intestine ◽  
Growth Performances ◽  
Intestinal Morphometry

Fishmeal (FM) is still the most important protein source in aquafeeds. However, due to the reduction of wild fish stocks used for FM production, its manufacturing it is now unsustainable. Insect meal represents a valid alternative to FM, due to the low carbon footprint of its production and its high nutritional value. The aim of this study was to investigate the potentials of replacing FM with black soldier fly (Hermetia illucens) meal (HIM) in aquafeeds, using zebrafish as the animal model. Four diets were formulated with increasing HIM/FM replacement rate (the control diet contained 20% FM). Mortality rates, growth performances, and feed consumptions were measured, and fish intestine samples were collected for histological and enzymatic analysis. After 49 days, all groups almost tripled their initial body weight (cumulative body weight gain ranged between 143.9 and 155.2 mg), and no statistically significant differences among treatments were observed in relation to growth performances and histological traits. Contrarily, trypsin, alkaline phosphatases, and alpha amylase–glucoamylase activities were significantly reduced when the FM replacement rate was increased. In conclusion, HIM may represent an alternative to FM, since no adverse effects were observed when it was included up to 20% in the zebrafish diet, even when replacing 100% of the FM.

Protein supplementation of silage for ewes in late pregnancy

1996 ◽  
Vol 1996 ◽  
pp. 47-47
Author(s):  
J.E. Vipond ◽  
M. Lewis ◽  
G.M. Povey
Keyword(s):  
Experimental Evidence ◽  
Litter Size ◽  
Crude Protein ◽  
Late Pregnancy ◽  
Protein Supplementation ◽  
Energy Requirements ◽  
Grass Silage ◽  
Condition Score ◽  
Low Levels ◽  
The Uk

Ewes fed good quality grass silage need low levels (0.4-0.6 kg/d) of concentrate supplement to satisfy energy requirements in late pregnancy. However, the UK Metabolisable Protein (MP) system predicts that using a low level of a typical 180 g/kg crude protein (CP) compound will result in an undersupply of MP and therefore a higher digestible undegradable protein (DUP) content of compounds is required. Although the benefits of supplying additional DUP to lactating ewes are well established there is little or no experimental evidence to support the practice of supplementing silage based diets with supplementary DUP. The objective of the experiment was to evaluate the response to supplementary DUP in silage based diets.One hundred and twenty five scanned Scotch Mule ewes were synchronised, mated to Texel rams and allocated to 5 treatments balanced for liveweight, condition score, litter size, and parity. Five supplements were formulated to supply varying amounts of DUP and eRDP.

Stretch-induced growth in chicken wing muscles: a new model of stretch hypertrophy

1980 ◽  
Vol 238 (1) ◽  
pp. C62-C71 ◽  
Author(s):  
R. G. Holly ◽  
J. G. Barnett ◽  
C. R. Ashmore ◽  
R. G. Taylor ◽  
P. A. Mole
Keyword(s):  
Cross Section ◽  
Muscle Activity ◽  
Enzyme Activities ◽  
Latissimus Dorsi ◽  
Capillary Density ◽  
Oxidative Enzyme ◽  
Longitudinal Growth ◽  
Mitochondrial Enzyme ◽  
New Model ◽  
Anterior Latissimus Dorsi

A new model of stretch-induced growth is evaluated in four chicken wing muscles stretched to different extents by a spring-loaded tubular assembly. Muscles grew in length and cross section in proportion to the extent to which they were stretched. Longitudinal growth was essentially completed within 1 wk, while muscles grew in cross section through at least 5 wk of stretch. The muscles were neither denervated nor immobilized, and muscle activity as measured by EMG was not increased. Oxidative enzyme activities increased substantially with stretch in the patagialis (PAT), a twitch muscle, but were relatively unchanged in the slow-tonic anterior latissimus dorsi (ALD). Stretch altered mitochondrial enzyme proportions in the PAT, but had little effect in the ALD. Capillary density was unchanged with stretch in the PAT, but decreased in the ALD. Capillary density was unchanged with stretch in the PAT, but decreased in the ALD. Capillary-to-fiber ratio, however, increased in both muscles. We conclude that muscles grow and adapt enzymatically due to stretch, but that these responses are dissimilar in twitch and tonic muscles.

scholarly journals Kerevit (Astacus leptodactylus) Yemine Katılan Selenyumun Paraoksonaz ve Arilesteraz Enzim Aktivitelerine Etkisi

2019 ◽  
Vol 7 (2) ◽  
pp. 240
Author(s):  
Serpil Mişe Yonar ◽  
Muzaffer Harlıoğlu
Keyword(s):  
Total Energy ◽  
Enzyme Activities ◽  
Crude Protein ◽  
Energy Levels ◽  
Egg Laying ◽  
Control Group ◽  
Astacus Leptodactylus ◽  
Tissue Samples ◽  
Positive Effects ◽  
Egg Carrying

In this study, the effects of selenium added to the diets of Astacus leptodactylus at different ratios on paraoxonase (PON) and arylesterase enzyme activities (ARE) in the hepatopancreas and gonad tissues were investigated. In this study, control (K), trial 1 (D1), trial 2 (D2) and trial 3 (D3) were prepared at the selenium levels of 0,3, 0,6, 0,9 and 1,2 mg/kg, respectively. The crude protein and total energy levels of experimental diets were equalized. 12 ponds in 2 × 2 × 1 m dimensions were used. 75 female and 25 male crayfish were stocked in each pond (totally 1200 crayfish). This study was carried out in triplicate. The crayfish were fed twice a day during 9 months. PON and ARE enzyme activities were investigated in the tissue samples taken monthly from the crayfish. During the trial, significant differences were observed in the PON and ARE enzyme activities in the hepatopancreas and gonad tissues. The PON and ARE enzyme activities increased statistically significant in the crayfish tissues during the breeding season and incubation period. This increase was found to be statistically different in the D1, D2 and D3 groups compared to the control group. In conclusion, selenium had positive effects on PON and ARE enzyme activities of A.leptodactylus during its mating, pleopodal egg laying and pleopodal egg carrying periods.

Maggot Black Soldier Fly (Hermetia illucens) Nutritional Content Using Various Culture Media

2021 ◽  
Vol 8 (3) ◽  
pp. 202-211
Author(s):  
Ase Lestari ◽  
T.H Wahyuni ◽  
T.H Wahyuni ◽  
E. Mirwandhono ◽  
N. Ginting
Keyword(s):  
Rice Bran ◽  
Crude Protein ◽  
Culture Media ◽  
Palm Kernel ◽  
Ash Content ◽  
Palm Kernel Cake ◽  
Nutritional Content ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
The Media

Maggot black soldier fly (Hermetia illucens) is an insect that is environmentally friendly and can be mass produced and does not compete with humans. In addition, insect cultivation can reduce organic waste that has the potential to pollute the environment. Maggot black soldier fly (Hermetia illucens) has a high crude protein content of 42.1%. The length of the life cycle and the high and low nutrient content of the maggot are influenced by the maggot growing media used. In this study using EM4 as a bio-activator for fermentation of rice bran, coconut dregs, tofu dregs and palm kernel cake. The function of this fermentation is to increase the nutrients contained in this material which will later be used as a growing medium for maggot and maggot is one of the alternatives to feed protein sources from fish meal. The study was conducted experimentally using a completely randomized design (CRD) 5 treatments and 4 replications, with the weight of each experimental unit the media content is 1 kg and filled with 1 g of BSF eggs and the media size is 37 cm long, 26 cm wide and 10 cm high. The parameters of this study consisted of maggot production and maggot nutritional content, namely moisture content, ash content, crude protein, crude fat and carbohydrates. Based on research results P0 (100% fermented rice bran) obtained an average maggot production of 525g, KA maggot 8,16%, KAb maggot 7,83%, PK maggot 44,82%, LK maggot 21,62% and 17.58% maggot carbohydrates respectively. P1 (50% fermented rice bran + 50% fermented coconut dregs) obtained an average maggot production of 467.5g, KA maggot 9,57%, KAb maggot 9,03%, PK maggot 44,68%, LK maggot 20,51% and 16.20% maggot carbohydrates respectively. P2 (50% fermented rice bran + 50% fermented tofu dregs) obtained an average maggot production of 545.25g, KA maggot 9,02%, KAb maggot 8,32%, PK maggot 44,74%, LK maggot 18,57% and 20.09% maggot carbohydrates respectively. P3 (50% fermented rice bran + 50% palm kernel cake) obtained an average maggot production of 542g, KA maggot 9,16%, KAb maggot 9,22%, PK maggot 43,72%, LK maggot 19,87% and 18.02% maggot carbohydrates respectively. P4 (25% fermented rice bran + 25% fermented tofu dregs + 25% fermented coconut dregs + 25% palm kernel cake) obtained an average maggot production of 831.5g, KA maggot 9,08%, KAb maggot 9,35%, PK maggot 44,38%, LK maggot 17,01% and 20.18% maggot carbohydrates respectively. From the research results, it can be concluded that the use of culture media with a combination of 25% fermented rice bran + 25% fermented coconut dregs + 25% fermented tofu dregs + 25% fermented palm kernel meal is the best combination where the production reaches 831.5 g bsf and 20 carbohydrates. , 18% but could not increase moisture content, ash content, crude protein and crude fat.

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