Evaluation of a Solid Dispersion of Curcumin With Polyvinylpyrrolidone and Boric Acid Against Salmonella Enteritidis Infection and Intestinal Permeability in Broiler Chickens: A Pilot Study

Two experiments were conducted to evaluate the effect of the prophylactic or therapeutic administration of a 0.1% mixture containing ascorbic acid and a solid dispersion of curcumin with polyvinylpyrrolidone and boric acid (AA-CUR/PVP-BA) against Salmonella Enteritidis (S. Enteritidis) in broiler chickens. A third experiment was conducted to evaluate the impact of the dietary administration of 0.1% AA-CUR/PVP-BA in a necrotic enteritis (NE) model in broiler chickens. The prophylactic administration of 0.1% AA-CUR/PVP-BA significantly decreased S. Enteritidis colonization in cecal tonsils (CT) when compared to the positive control group (PC, p < 0.05). The therapeutic administration of 0.1% AA-CUR/PVP-BA significantly reduced the concentration of S. Enteritidis by 2.05 and 2.71 log in crop and CT, respectively, when compared with the PC on day 10 post-S. Enteritidis challenge. Furthermore, the serum FITC-d concentration and total intestinal IgA levels were also significantly lower in chickens that received 0.1% AA-CUR/PVP-BA. Contrary, the PC group showed significantly higher total intestinal IgA levels compared to the negative control or AA-CUR/PVP-BA groups in the NE model. However, 0.1% AA-CUR/PVP-BA showed a better effect in reducing the concentration of S. Enteritidis when compared to the NE model. Further studies with higher concentration of AA-CUR/PVP-BA into the feed to extend these preliminary results are currently being evaluated.

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Evaluation of the antimicrobial and intestinal integrity properties of boric acid in broiler chickens infected with Salmonella enteritidis: Proof of concept

Research in Veterinary Science ◽

10.1016/j.rvsc.2018.12.004 ◽

2019 ◽

Vol 123 ◽

pp. 7-13 ◽

Cited By ~ 5

Author(s):

Daniel Hernandez-Patlan ◽

Bruno Solis-Cruz ◽

Bishnu Adhikari ◽

Karine P. Pontin ◽

Juan D. Latorre ◽

...

Keyword(s):

Boric Acid ◽

Broiler Chickens ◽

Salmonella Enteritidis ◽

Proof Of Concept ◽

Intestinal Integrity

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The effect of moderate-dose aflatoxin B1 and Salmonella Enteritidis infection on intestinal permeability in broiler chickens

Mycotoxin Research ◽

10.1007/s12550-019-00367-7 ◽

2019 ◽

Vol 36 (1) ◽

pp. 31-39

Author(s):

J. O. Hernández-Ramírez ◽

M. J. Nava-Ramírez ◽

R. Merino-Guzmán ◽

G. Téllez-Isaías ◽

A. Vázquez-Durán ◽

...

Keyword(s):

Intestinal Permeability ◽

Aflatoxin B1 ◽

Broiler Chickens ◽

Salmonella Enteritidis ◽

Moderate Dose

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Evaluation of Ascorbic Acid or Curcumin Formulated in a Solid Dispersion on Salmonella Enteritidis Infection and Intestinal Integrity in Broiler Chickens

Pathogens ◽

10.3390/pathogens8040229 ◽

2019 ◽

Vol 8 (4) ◽

pp. 229 ◽

Cited By ~ 4

Author(s):

Daniel Hernandez-Patlan ◽

Bruno Solis-Cruz ◽

Karine P. Pontin ◽

Juan D. Latorre ◽

Xochitl Hernandez-Velasco ◽

...

Keyword(s):

Ascorbic Acid ◽

Solid Dispersion ◽

Broiler Chickens ◽

Salmonella Enteritidis ◽

Fluorescein Isothiocyanate ◽

Positive Control ◽

Experimental Models ◽

Sod Activity ◽

Intestinal Integrity ◽

Therapeutic Alternatives

Two experimental models were conducted to evaluate and compare the effect of ascorbic acid (AA) or curcumin formulated in a solid dispersion (SD-CUR) as prophylactic or therapeutic alternatives to prevent or control S. Enteritidis (SE) infection in broiler chickens. In the prophylactic model, dietary administration of AA showed a significant reduction in SE counts in crop compared to the positive control (PC) group (p < 0.05), whereas in cecal tonsils (CT), SD-CUR significantly reduced SE recovery. Superoxide dismutase (SOD) activity was significantly higher in chickens supplemented with AA or SD-CUR, and total intestinal IgA levels were significantly lower in both treatments when compared to the PC group. Serum fluorescein isothiocyanate-dextran (FITC-d) levels were reduced by SD-CUR compared to PC, while AA presented significantly lower total aerobic bacteria. In the therapeutic model, only the dietary administration of AA significantly decreased SE in crop and CT on days 3 and 10 post-challenge. FITC-d levels were significantly lower in both treated groups in comparison to PC, but IgA levels were significantly reduced only by AA. The results suggest that dietary AA and SD-CUR have different modes of action to reduce SE intestinal colonization in two different challenge models in broiler chickens.

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Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima

Scientific Reports ◽

10.1038/s41598-021-90679-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Victor M. Petrone-Garcia ◽

Raquel Lopez-Arellano ◽

Gabriela Rodríguez Patiño ◽

Miriam Aide Castillo Rodríguez ◽

Daniel Hernandez-Patlan ◽

...

Keyword(s):

Pilot Study ◽

Broiler Chickens ◽

Solid Phase ◽

Oxidation Products ◽

Specific Pathogen Free ◽

Post Challenge ◽

Eimeria Maxima ◽

Lipid Oxidation Products ◽

Challenge Control ◽

Specific Pathogen

AbstractThe purpose of this pilot study was to evaluate and determine the concentration of prostaglandin GF2α (PGF2α) and isoprostane 8‐iso‐PGF2α in plasma and intestine of specific pathogen-free (SPF) Leghorn chickens challenged with Eimeria maxima, with or without dietary supplementation of curcumin using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Eighty 1-day-old male SPF chickens were randomly allocated to one of four groups with four replicates (n = 5 chickens/replicate). Groups consisted of: (1) Control (no challenge), (2) Curcumin (no challenge), (3) Eimeria maxima (challenge), and (4) Eimeria maxima (challenge) + curcumin. At day 28 of age, all chickens in the challenge groups were orally gavaged with 40,000 sporulated E. maxima oocysts. No significant differences (P > 0.05) were observed in the groups regardless of the treatment or challenge with E. maxima. Enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days and 9 days post-challenge were significantly increased (P < 0.01) compared to the non-challenge control chickens. Interestingly, the enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days post-challenge were significantly reduced in chickens fed curcumin, compared to control chickens challenge with E. maxima. At 9 days post-challenge, only levels of isoprostane 8‐iso‐PGF2α in the enteric samples were significantly reduced in chickens challenged with E. maxima supplemented with curcumin, compared with E. maxima challenge chickens. No differences of isoprostane 8‐iso‐PGF2α or PGF2α were observed in plasma at both days of evaluation. Similarly, no significant differences were observed between the challenge control or chickens challenge with E. maxima and supplemented with curcumin at both times of evaluation. The results of this pilot study suggests that the antioxidant anti-inflammatory properties of curcumin reduced the oxidative damage and subsequent intestinal mucosal over-production of lipid oxidation products. Further studies to confirm and extend these results in broiler chickens are required.

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Improved Hygroscopicity and Bioavailability of Solid Dispersion of Red Ginseng Extract with Silicon Dioxide

Pharmaceutics ◽

10.3390/pharmaceutics13071022 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1022

Author(s):

Sojeong Jin ◽

Chul Haeng Lee ◽

Dong Yu Lim ◽

Jaehyeok Lee ◽

Soo-Jin Park ◽

...

Keyword(s):

Silicon Dioxide ◽

Intestinal Permeability ◽

Solid Dispersion ◽

Ussing Chamber ◽

Red Ginseng ◽

Scanning Calorimetry ◽

Korean Red Ginseng ◽

Ginseng Extract ◽

Powder Formulation ◽

Significant Difference

This study aims to develop a powder formulation for the Korean red ginseng extract (RGE) and to evaluate its in vitro and in vivo formulation characteristics. The solid dispersion of RGE was prepared with hydrophilic carriers using a freeze-drying method. After conducting the water sorption–desorption isothermogram (relative humidity between 30 and 70% RH), differential scanning calorimetry thermal behavior, dissolution test, and intestinal permeation study, a solid dispersion formulation of RGE and silicon dioxide (RGE-SiO2) was selected. RGE-SiO2 formulation increased intestinal permeability of ginsenoside Rb1 (GRb1), GRb2, GRc, and GRd by 1.6-fold in rat jejunal segments as measured by the Ussing chamber system. A 1.6- to 1.8-fold increase in plasma exposure of GRb1, GRb2, GRc, and GRd in rats was observed following oral administration of RGE-SiO2 (375 mg/kg as RGE). No significant difference was observed in the time to reach maximum concentration (Tmax) and half-life in comparison to those in RGE administered rats (375 mg/kg). In conclusion, formulating solid dispersion of RGE with amorphous SiO2, the powder formulation of RGE was successfully formulated with improved hygroscopicity, increased intestinal permeability, and enhanced oral bioavailability and is therefore suitable for processing solid formulations of RGE product.

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Use of a sensitive multi‐sugar test for measuring segmental intestinal permeability in critically ill mechanically ventilated adults: A pilot study

Journal of Parenteral and Enteral Nutrition ◽

10.1002/jpen.2110 ◽

2021 ◽

Author(s):

Oana A Tatucu‐Babet ◽

Adrienne Forsyth ◽

Andrew Udy ◽

Jessica Radcliffe ◽

Devin Benheim ◽

...

Keyword(s):

Pilot Study ◽

Critically Ill ◽

Intestinal Permeability ◽

Mechanically Ventilated

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Prevention of colonization by Salmonella enteritidis PT4 in broiler chickens

International Journal of Food Microbiology ◽

10.1016/0168-1605(92)90064-a ◽

1992 ◽

Vol 15 (3-4) ◽

pp. 313-317 ◽

Cited By ~ 15

Author(s):

N.M. Bolder ◽

L.A.J.T. van Lith ◽

F.F. Putirulan ◽

W.F. Jacobs-Reitsma ◽

R.W.A.W. Mulder

Keyword(s):

Broiler Chickens ◽

Salmonella Enteritidis

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Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in Poultry Carcasses and Different Types of Poultry Products for Sale on the Belgian Retail Market

Journal of Food Protection ◽

10.4315/0362-028x-62.7.735 ◽

1999 ◽

Vol 62 (7) ◽

pp. 735-740 ◽

Cited By ~ 108

Author(s):

M. UYTTENDAELE ◽

P. DE TROY ◽

J. DEBEVERE

Keyword(s):

Listeria Monocytogenes ◽

Campylobacter Jejuni ◽

Broiler Chickens ◽

Salmonella Enteritidis ◽

Contamination Level ◽

Contamination Rate ◽

Campylobacter Coli ◽

Retail Market ◽

Salmonella Spp ◽

Poultry Products

From January 1997 to May 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in Belgium were analyzed for the presence of Salmonella spp., Salmonella Enteritidis, Campylobacter jejuni, C. coli, and Listeria monocytogenes per 100 cm2 or 25 g. Poultry samples were contaminated with Salmonella (36.5%), C. jejuni and C. coli (28.5%), and L. monocytogenes (38.2%). In about 12.3% of the poultry samples, the L. monocytogenes contamination level exceeded 1 CFU per g or cm2. Significant differences in pathogen contamination rates of poultry products were noticed between the poultry products originating from Belgian, French, and U.K. abattoirs. Poultry products derived from broiler chickens running free in pine woods until slaughtering age (12 to 13 weeks) had a significantly (P < 0.05) lower contamination rate of Salmonella than poultry products from enclosed broilers slaughtered at the age of 6 to 8 weeks. A significantly (P < 0.05) lower pathogen contamination rate was noted for Salmonella, C. jejuni, and C. coli for poultry cuts without skin compared to poultry cuts with skin on. An increase in pathogen contamination rate was noticed during cutting and further processing. To diminish C. jejuni, C. coli, Salmonella, and L. monocytogenes contamination rates, hygienic rules of slaughter and meat processing must be rigorously observed. At the moment, zero tolerance for these pathogens is not feasible, and there is a need to establish criteria allowing these pathogens to be present at reasonable levels in the examined poultry samples.

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Intestinal Barrier Function and Performance of Broiler Chickens Fed Additional Arginine, Combination of Arginine and Glutamine or an Amino Acid-Based Solution

Animals ◽

10.3390/ani11082416 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2416

Author(s):

Reza Barekatain ◽

Tristan Chalvon-Demersay ◽

Clive McLaughlan ◽

William Lambert

Keyword(s):

Amino Acid ◽

Intestinal Permeability ◽

Broiler Chickens ◽

Intestinal Inflammation ◽

Intestinal Barrier ◽

Basal Diet ◽

Intestinal Barrier Function ◽

Glutathione Synthetase ◽

Feed Conversion ◽

Experimental Treatments

Two experiments were conducted to investigate the effect of arginine (Arg); the combination of Arg and glutamine (Gln); as well as an amino acid-based solution (MIX) containing Arg, Gln, threonine (Thr), and grape extract, on performance, intestinal permeability, and expression of selected mechanistic genes. Using 240 male Ross 308 off-sex broiler chickens, four experimental treatments were replicated six times with 10 birds per replicate. The experimental treatments included 5 g/kg Arg, 2.5 g/kg Arg and 2.5 g/kg Gln, and 1 g/kg MIX added to a basal diet as control. In the second study, the four dietary treatments were then given to 24 birds with or without a synthetic glucocorticoid, dexamethasone (DEX), as a gut dysfunction model. Feed conversion ratio was improved by all the supplemented treatments from day 7 to 35 of age (p < 0.001). DEX injections increased (p < 0.001) the intestinal permeability in all treatments, which tended to be reversed by Arg or MIX. Additional Arg, Arg-Gln, and MIX suppressed (p < 0.05) the overexpression of IL-1β generated by DEX. Feeding birds with MIX treatment increased (p < 0.05) expression of SGLT-1 and glutathione synthetase. In conclusion, tested amino acid supplements were effective in improving feed efficiency and restraining intestinal inflammation caused by DEX through IL-1β pathway.

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