scholarly journals Molecular Epidemiology and Characteristics of CTX-M-55 Extended-Spectrum β-Lactamase-Producing Escherichia coli From Guangzhou, China

2021 ◽  
Vol 12 ◽  
Author(s):  
Shihan Zeng ◽  
Jiajun Luo ◽  
Xiaoyan Li ◽  
Chao Zhuo ◽  
Aiwu Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Epidemiology ◽  
Pcr Amplification ◽  
Common Structure ◽  
Extended Spectrum ◽  
The Future ◽  
Positive Rate ◽  
Amoxicillin Clavulanate ◽  
Sensitivity Rate ◽  
Resistance Rates

In recent years, the CTX-M-55 extended-spectrum β-lactamase (ESBL)-positive rate has gradually increased in the clinic. To identify the molecular epidemiology and characteristics of blaCTX–M–55-positive isolates, a total of 374 non-repetitive ESBL-producing Escherichia coli strains were collected from patients in two hospitals in Guangzhou, and 89 blaCTX–M–55-positive isolates were selected by CTX-M-1-group PCR amplification and confirmed by DNA sequencing. Whole-genome sequencing was used to analyze the resistance phenotype, plasmid types, phylogenetic relationships and genetic environment of the blaCTX–M–55 gene. Conjugation experiments and PCR were performed to confirm whether the plasmid harboring blaCTX–M–55 gene could be transferred. The results showed that all blaCTX–M–55-positive isolates were resistant to ceftriaxone, and 88.76 and 76.40% were resistant to ceftazidime and cefepime, respectively. The resistance rates to levofloxacin and sulfamethoxazole were 66.29 and 59.55%, respectively. However, the sensitivity rate of piperacillin/tazobactam, amoxicillin/clavulanate, and amikacin exceeded 90%. All blaCTX–M–55-positive isolates were sensitive to carbapenems. Thirty-two STs were detected in the blaCTX–M–55-positive isolates, among which the detection rate of ST1193 was relatively high (19.10%, 17/89), and other ST types were scattered. It remains to be seen whether ST1193 carrying the blaCTX–M–55 gene can become a popular clone strain in this region in the future. The plasmid types carrying the blaCTX–M–55 gene included IncI1, IncFII, IncFIC, IncFIB, IncHI2, IncI2, and IncX/Y, among which the IncI1 and IncFII plasmids were the main plasmids, accounting for 37.80 and 28.09%, respectively. Among them, 11 strains of the IncI1 plasmid existed in ST1193 strains. The blaCTX–M–55 gene was found on chromosomes of 13 isolates, and seemed to be increasing annually. Up to five distinct types of genetic environments surrounding the blaCTX–M–55 gene were analyzed. The most common structure was type II “ISEcp1-blaCTX–M–55-ORF477.” In conclusion, whether ST1193, which carries blaCTX–M–55 gene, will be an epidemic clone of this region in the future remains to be concerned. The plasmids IncI1 and IncFII, and mobile elements such as ISEcp1 and IS26 may be the main factors leading to the spread and prevalence of CTX-M-55 genotypes.

scholarly journals Prevalence and Molecular Epidemiology of Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli from Multiple Sectors of Poultry Industry in Korea

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1050
Author(s):  
Hyunsoo Kim ◽  
Young Ah Kim ◽  
Young Hee Seo ◽  
Hyukmin Lee ◽  
Kyungwon Lee
Keyword(s):  
Escherichia Coli ◽  
Molecular Epidemiology ◽  
Core Gene ◽  
Common Type ◽  
Poultry Industry ◽  
Extended Spectrum ◽  
Positive Rate ◽  
Human Use ◽  
M 14 ◽  
Core Genes

The aim of this study was to investigate the molecular epidemiology of extended-spectrum-β-lactamase producing Escherichia coli (ESBL-EC) from poultry, the poultry farm environment, and workers in Korea. A total of 1376 non-duplicate samples were collected from 21 poultry farms, 20 retail stores, 6 slaughterhouses, and 111 workers in a nationwide study in Korea from January 2019 to August 2019. The overall positive rate of ESBL-EC was 6.8%, with variable positive rates according to sources (0.9% of worker, 5.2% of poultry, 10.0% of chicken meat, and 14.3% of environment). Common ESBL types were CTX-M-55 and CTX-M-14 in a total of 93 ESBL-EC isolates. Whole genome sequencing revealed that 84 ESBL-EC isolates had an outstanding accumulation of numerous antimicrobial resistance (AMR) genes associated with resistance to various classes of antimicrobials for human use and well-known antimicrobial gene (ARG)-carrying plasmids. Core gene multi locus sequence typing, using 2390 core genes, indicated no dominant clone or common type in each province. In conclusion, the isolation rates of ESBL-EC were not negligible in the poultry industry-related samples, sharing common ESBL types of human ESBL-EC isolates in Korea.

scholarly journals Antimicrobial resistance of Escherichia coli isolates from cattle in Eastern Algeria

2019 ◽  
Vol 12 (8) ◽  
pp. 1195-1203 ◽  
Author(s):  
Djanette Barour ◽  
Amine Berghiche ◽  
Nadji Boulebda
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Diffusion Method ◽  
E Coli ◽  
Extended Spectrum ◽  
Amoxicillin Clavulanate ◽  
Eastern Algeria ◽  
Resistance Rates

Background and Aim: Lack of information about the antibiotic resistance in commensal Escherichia coli from Algerian livestock prompted us to do this study to determine the different levels of antimicrobial susceptibility, antibiotic multidrug resistance (MDR) rates, and phenotypical patterns of E. coli strains isolated from healthy cattle to control the spread of animal-resistant strains to humans and the environment. Materials and Methods: A total of 198 cattle were sampled (swabbed in the rectum), reared in the farms of Souk Ahras, Tebessa, and Oum el Bouaghi governorates of Eastern Algeria. Isolation of E. coli strains was performed on MacConkey agar and then the different strains were identified to the species level using an API 20E identification kit. Antimicrobial susceptibility was determined using a panel of 13 antibiotic disks by disk diffusion method on Mueller-Hinton agar. The double-disk synergy test with cefotaxime and amoxicillin-clavulanate disks was used for the screening of extended-spectrum beta-lactamase phenotypes. For colistin susceptibility, the minimum inhibitory concentration was examined using broth microdilutions technique. Results: The results showed that among the 198 E. coli isolates, elevated resistance rates were observed for ampicillin (59.09%) and tetracycline (43.43%), and moderate resistance rates for cephalothin (16.16%), trimethoprim/sulfamethoxazole (15.15%), and amoxicillin/clavulanate (11.62%); however, low resistance rates were found for nalidixic acid (8.08%), ciprofloxacin (7.07%), kanamycin (6.56%), cefotaxime (4.54%), chloramphenicol (4.04%), nitrofurantoin (2.52%), cefoxitin (2.02%), gentamycin (1.01%), and no resistance to colistin. However, nine extended-spectrum β-lactamases producing E. coli strains were identified. Forty-four different patterns were determined, indicating a wide variety of resistance, ranging from one antimicrobial to a combination of 10. Analysis of coresistances revealed that 63 isolates (31.82%) were susceptible to all antibiotics used in the study, 42 isolates (21.21%) were resistant to one antibiotic, 43 isolates (21.72%) were resistant to two antibiotics, 24 isolates (12.12%) resistant to three antibiotics, 26 isolates (13.13%) were resistant for more than three agents, and 45 isolates (22.73%) were MDR (which means resistant to three or more families of antibiotics). Conclusion: This study demonstrates that commensal E. coli remains a potential source of antibiotic resistance in view of the high prevalence of antimicrobial resistance. The vast range of MDR phenotypes, especially extended-spectrum β-lactamases producing strains, emphasizes the urgent requirement to adopt measures to control the use of antimicrobials, in particular, by private veterinarians, as well as the strengthening of veterinary surveillance networks for antimicrobial resistance to control the spread of MDR bacteria from animals to humans and the environment.

scholarly journals Extraintestinal pathogenic Escherichia coli in Saudi Arabia: A review of antimicrobial resistance and molecular epidemiology

2020 ◽  
Vol 19 (2) ◽  
pp. 447-453
Author(s):  
Abdulaziz Alqasim
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
Antimicrobial Resistance ◽  
Molecular Epidemiology ◽  
Bloodstream Infections ◽  
Epidemiological Studies ◽  
Colistin Resistance ◽  
E Coli ◽  
Extended Spectrum ◽  
Pathogenic Escherichia Coli

Extra-intestinal pathogenic Escherichia coli (ExPEC) is commonly associated with causing urinary tract and bloodstream infections. Over the past two decades, the antimicrobial resistance of ExPEC has increasingly been reported [1]. Given that Saudi Arabia annually hosts mass religious events, such as Hajj, this review investigated several aspects of antimicrobial resistance of ExPEC in this country including the current prevalence of resistance and molecular epidemiology of ExPEC isolates. Generally, the overall prevalence of antibiotic resistance of ExPEC in Saudi Arabia is on increase. The current emergence of colistin resistance in ExPEC represents a major challenge to public health. Local molecular epidemiological studies have shown the dominance of E. coli sequence type 131 (E. coli ST131) over other major ExPEC STs. This is an important observation given that this clone has been associated with high multidrug resistance and extended-spectrum β-lactamases carriage. To reduce the burden of this resistance in the future, it would be crucial to avoid uncontrolled use of antibiotics in either clinical settings or animal food industry. Keywords: Extra-intestinal pathogenic Escherichia coli, Antimicrobial resistance, ST131, Saudi Arabia, Colistin resistance, Extended-spectrum β-lactamases

scholarly journals Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Hospitalized Neonatal Foals: Prevalence, Risk Factors for Shedding and Association with Infection

Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 600 ◽  
Author(s):  
Anat Shnaiderman-Torban ◽  
Yossi Paitan ◽  
Haia Arielly ◽  
Kira Kondratyeva ◽  
Sharon Tirosh-Levy ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Species Diversity ◽  
Multilocus Sequence Typing ◽  
Sanger Sequencing ◽  
Medical Data ◽  
Esbl Production ◽  
Extended Spectrum ◽  
Resistance Rates ◽  
Susceptibility Profiles

Extended-spectrum β-lactamase Enterobacteriaceae (ESBL-E) have been investigated in adult horses, but not in foals. We aimed to determine shedding and infection in neonatal foals and mares. Rectal swabs were sampled from mare and foal pairs on admission and on the 3rd day of hospitalization; enriched, plated, and bacteria were verified for ESBL production. Identification and antibiotic susceptibility profiles were determined (Vitek2). Genotyping was performed by multilocus sequence typing (MLST). Genes were identified by PCR and Sanger sequencing. Medical data were analyzed for risk factors (SPSS). On admission, 55 pairs were sampled, of which 33 pairs were re-sampled. Shedding rates on admission in foals and mares were 33% (95% CI 21–47%) and 16% (95% CI 8–29%), respectively, and during hospitalization, these increased significantly to 85% (95% CI 70–94%) and 58% (95% CI 40–73%), respectively. Foal shedding was associated with umbilical infection on admission (P = 0.016) and with ampicillin treatment during hospitalization (p = 0.011), and was independent of the mare’s shedding. The most common ESBL-E was Escherichia coli. During hospitalization, species diversity increased. Four foals were infected with ESBL-E strains, including umbilical infections and wounds. This study substantiates an alarming prevalence of shedding in neonatal foals, which should be further investigated in order to reduce resistance rates.

scholarly journals Oral and Parenteral Therapeutic Options for Outpatient Urinary Infections Caused by Enterobacteriaceae Producing CTX-M Extended-Spectrum β-Lactamases

2009 ◽  
Vol 53 (3) ◽  
pp. 1278-1280 ◽  
Author(s):  
Vidhya Prakash ◽  
James S. Lewis ◽  
Monica L. Herrera ◽  
Brian L. Wickes ◽  
James H. Jorgensen
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Agents ◽  
Therapeutic Options ◽  
Extended Spectrum ◽  
Urinary Infections ◽  
Amoxicillin Clavulanate ◽  
Long Acting ◽  
Tract Infections ◽  
Community Onset ◽  
Urinary Isolates

ABSTRACT Effective therapeutic options are needed for community-onset urinary tract infections due to Escherichia coli strains that produce CTX-M extended-spectrum β-lactamases. We examined 46 urinary isolates producing CTX-M against several oral or long-acting parenteral antimicrobial agents. Approximately 90% were susceptible to fosfomycin and to a combination of cefdinir plus amoxicillin-clavulanate. All were susceptible to ertapenem.

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