scholarly journals The Response of Critical Microbial Taxa to Litter Micro-Nutrients and Macro-Chemistry Determined the Agricultural Soil Priming Intensity After Afforestation

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongling Yang ◽  
Yulin Li ◽  
Shaokun Wang ◽  
Jin Zhan ◽  
Zhiying Ning ◽  
...  
Keyword(s):  
Litter Quality ◽  
Agricultural Soils ◽  
Condensed Tannin ◽  
Soluble Sugar ◽  
Fungal Species ◽  
Carbon Accumulation ◽  
Water Soluble ◽  
Farmland Soil ◽  
Litter Mixture ◽  
Soc Mineralization

Afforestation with trees and shrubs around cropland can effectively decrease soil degradation and avoid sand storms, but subsequent modification of litter quality accelerates the degradation of native organic matter via the soil priming effect (PE). Although carbon accumulation in agricultural soils after afforestation was widely studied, little is known about the extent to which soil organic carbon (SOC) mineralization is induced by complex residue input in agro-forest-grass composite ecosystems. Here, we mixed corn field soil and litter of afforestation tree and shrub species together in a micro-environment to quantify the effects of litter-mixture input on farmland soil priming associated with afforestation. Additionally, we studied the responses of bacterial and fungal species to litter chemistry, with the aim to identify the litter and microbial driver of soil priming. The results showed that soil priming was accelerated by different litter addition which varied from 24 to 74% of SOC mineralization, suggesting that priming intensity was relatively flexible and highly affected by litter quality. We also find that the macro-chemistry (including litter carbon, nitrogen, lignin, and cellulose) directly affects priming intensity, while micro-chemistry (including litter soluble sugar, water-soluble phenol, methanol-soluble phenol, and condensed tannin) indirectly influences priming via alteration to dominant bacterial taxa. The stepwise regression analysis suggested that litter nitrogen and cellulose were the critical litter drivers to soil priming (r2 = 0.279), and the combination of bacterial phylum Proteobacteria, Firmicutes, Bacteroidetes, Acidobacteria, and fungal taxa Eurotiomycetes was a great model to explain the priming intensity (r2 = 0.407).

scholarly journals Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2596 ◽  
Author(s):  
Yongxia Cheng ◽  
Haibo Lan ◽  
Lei Zhao ◽  
Kai Wang ◽  
Zhuoyan Hu
Keyword(s):  
Soluble Sugar ◽  
Monosaccharide Composition ◽  
Water Soluble ◽  
Lactobacillus Delbrueckii ◽  
Soluble Solids ◽  
Enzyme Treatment ◽  
Bacteria Growth ◽  
Before And After ◽  
Soluble Polysaccharide

The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated. The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment. The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro. The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment. The water-soluble polysaccharide content, including pectin, was significantly increased. Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content. Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment. The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment. The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.

The forms of potassium and potassium adsorption in some virgin soils from south-western Australia

Soil Research ◽  
1999 ◽  
Vol 37 (4) ◽  
pp. 695 ◽  
Author(s):  
Y. Pal ◽  
R. J. Gilkes ◽  
M. T. F. Wong
Keyword(s):  
Western Australia ◽  
Agricultural Soils ◽  
Clay Mineralogy ◽  
Sorption Isotherms ◽  
Clay Content ◽  
Water Soluble ◽  
X Ray Diffraction ◽  
Exchangeable K ◽  
The North ◽  
Southern District

This investigation was undertaken to determine the potassium (K) status and adsorption behaviour of 227 samples from horizons of 41 representative virgin soil profiles, extending from Geraldton in the north to the Great Southern district of Western Australia. X-ray diffraction analysis of random powder of whole soil indicated that quartz is the dominant mineral and some soils contain significant amounts of feldspars. Clay mineralogy is dominated by kaolinite but minor quantities of illite are present in some soils. Most south-west Australian agricultural soils contain little available K: NaHCO3-extractable K (NaHCO3-K, median value 0·09 cmol K/kg, equivalent to 35 mg K/kg soil), HNO3-extractable K (HNO3-K, median value 0·30 cmol K/kg, equivalent to 117 mg K/kg soil), and total K (XRF determined K, median value, 17 cmol K/kg, equivalent to 6630 mg K/kg soil). The proportion of water-soluble K (H2O-K) ranges from 0 to 3·5% of total K, 0 to 76% of HNO3-K, and 0 to nearly 100% of exchangeable K. Exchangeable K ranges from 0 to 100% (median value 37%) of HNO3-K. These are relatively high proportions of H2O-K and exchangeable K compared with soils from many other parts of the world. The amounts of all forms of K variously increase or decrease downwards in the soil profile depending on both clay content and mineralogy. The commonly assumed increase in K with clay content alone is invalid. The soils mostly have low-to-moderate values of K sorption capacity, ranging from 5% to 67% (median value 14%) of added K (initially 4·1 mM K/L, equivalent to 4·1 cmol K/kg). otassium sorption isotherms conform well to the Freundlich equation. The inability of the Langmuir equation to describe the data may indicate that there are several types of K sorption sites in these soils. The Gapon coefficient KG varied widely from 0·04 to 29·8 (L/mol) ½ [median value 5·4 (L/mol) ½ ].

scholarly journals Development of Murine Monoclonal Antibodies for the Immunohistochemical Diagnosis of Systemic Bovine Aspergillosis

1996 ◽  
Vol 8 (1) ◽  
pp. 68-75 ◽  
Author(s):  
H. E. Jensen ◽  
B. Aalbaek ◽  
P. Lind ◽  
H. V. Krogh ◽  
P. L. Frandsen
Keyword(s):  
Monoclonal Antibodies ◽  
Polyclonal Antibodies ◽  
Fungal Species ◽  
Cross Reactivity ◽  
Water Soluble ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunohistochemical Techniques ◽  
Immunohistochemical Diagnosis ◽  
Murine Monoclonal Antibodies

Murine monoclonal antibodies (MAbs) against water-soluble somatic antigens (WSSA) and the wall fraction (WF) from Aspergillus fumigatus were produced by fusion of splenocytes from immunized BALB/c mice with mouse myeloma X63-Ag 8.653 cells. The supernatants of in vitro cultured hybridomas were initially screened for reactivity with the WSSA and the WF from A. fumigatus and WSSA of other fungi in an enzyme-linked immunosorbent assay (ELISA). Supernatants reacting only with A. fumigatus antigens were subsequently screened for homologous and heterologous reactivity with immunohistochemical techniques using formalin-fixed, paraffin-embedded tissues from experimentally infected mice. Because of a high immunohistochemical reactivity with homologous fungi, 4 MAbs raised against A. fumigatus WSSA and WF were selected for a further evaluation of cross-reactivity (diagnostic specificity) in immunohistochemical and immunoblotting assays. In immunohistochemical assays, all MAbs raised against WSSA cross-reacted heavily with a number of other fungal species. All 4 MAbs (MAb-WF-AF-1-4) raised against the WF reacted strongly with hyphae of Aspergillus spp.; hyphae of Scedosporium apiospermum were also strongly labeled by MAb-WF-AF-3 and-4. The 2 specifically reacting MAbs (MAb-WF-AF-1 and-2) were of the IgM biotype and were precipitating, and in immunoblotting experiments both bound to a 106-kD antigen of the WF, whereas they did not bind to WSSA of A. fumigatus. One of the 2 aspergillosis-specific MAbs (MAb-WF-AF-1) was used to screen 145 mycotic lesions of cattle. The diagnoses on bovine lesions obtained by MAb-WF-AF-1 were compared with results based on reactivity with heterologously absorbed polyclonal antibodies and, for some lesions, to culture results. In the vast majority of lesions ( n = 133), the MAb-WF-AF-1 and the polyclonal anti-Aspergillus antibodies reacted in a similar pattern, i.e., positively in 41 aspergillosis lesions and negatively in 92 zygomycotic lesions. Hyphae in 3 of 12 lesions that were not stained by the polyclonal antibodies reacted with the specific MAb-WF-AF-1; i.e., aspergillosis was diagnosed. The characteristics of the 2 MAbs (MAb-WF-AF-1 and-2) raised against the WF of A. fumigatus in ELISA and immunoblotting and immunohistochemical assays justify their application for the in situ diagnosis of systemic aspergillosis of cattle.

Near-Infrared Hyperspectral Imaging Rapidly Detects the Decay of Postharvest Strawberry Based on Water-Soluble Sugar Analysis

2019 ◽  
Vol 12 (4) ◽  
pp. 936-946 ◽  
Author(s):  
Qiang Liu ◽  
Kangli Wei ◽  
Hui Xiao ◽  
Sicong Tu ◽  
Ke Sun ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Near Infrared ◽  
Soluble Sugar ◽  
Water Soluble ◽  
Sugar Analysis

Effects of various soil fungi and insecticides on the capacity of Mucor alternans to degrade DDT

1972 ◽  
Vol 18 (5) ◽  
pp. 553-560 ◽  
Author(s):  
J. P. E. Anderson ◽  
E. P. Lichtenstein
Keyword(s):  
Contaminated Soils ◽  
Soil Fungi ◽  
Fungal Spores ◽  
Fungal Growth ◽  
Fungal Species ◽  
Water Soluble ◽  
Metabolite Formation ◽  
Pure Cultures ◽  
Free Media ◽  
Related Compounds

Pure cultures of the fungus Mucor alternans, isolated from DDT-contaminated soils, were able to degrade DDT to water-soluble metabolites. After the addition of fungal spores to DDT-contaminated soils, however, the insecticide-degrading capacity of the fungus was no longer evident. Since under field conditions many species of fungi are simultaneously exposed to mixed residues of pesticidal chemicals, the effects of various species of soil fungi and of various insecticides on DDT degradation by M. alternans were investigated. Experiments were conducted to study the effect of nine fungal species, their stale cell-free media, and various insecticides and related compounds on the capacity of M. alternans to degrade 14C-DDT to water-soluble metabolites. It was found that several pure fungal cultures or some cell-free media, in which mycelia had grown, could also degrade the insecticide. In most cases, however, addition of one of the various fungi to 14C-DDT-treated M. alternans cultures resulted in a total depression of the appearance of water-soluble metabolites in the media. This was due to an accumulation of the metabolites in the mycelium of the other fungus or in an inhibition of metabolite formation. Addition of stale media from various fungi to 14C-DDT-treated M. alternans cultures had various effects on fungal growth and on the capacity of the fungus to degrade the insecticide. Among the insecticides and related compounds tested only lindane, parathion, and Dyfonate caused a reduction in DDT degradation by M. alternans, without severely reducing its vegetative growth.

Bipiperidine conjugates as soluble sugar surrogates in DNA-intercalating antiproliferative polyketides

2016 ◽  
Vol 52 (27) ◽  
pp. 4894-4897 ◽  
Author(s):  
Nico Ueberschaar ◽  
Florian Meyer ◽  
Hans-Martin Dahse ◽  
Christian Hertweck
Keyword(s):  
Soluble Sugar ◽  
Water Soluble ◽  
Antiproliferative Activities

1,4′-Bipiperidine-1′-carbamate residues were evaluated as sugar surrogates in daunorubicin and chartreusin, yielding water-soluble derivatives and prodrugs with dramatically improved antiproliferative activities.

Profiling and relationship of water-soluble sugar and protein compositions in soybean seeds

2016 ◽  
Vol 196 ◽  
pp. 776-782 ◽  
Author(s):  
Xiaomin Yu ◽  
Fengjie Yuan ◽  
Xujun Fu ◽  
Danhua Zhu
Keyword(s):  
Soluble Sugar ◽  
Water Soluble ◽  
Soybean Seeds ◽  
Relationship Of

Synthesis of sugar-organometallic conjugales: ferrocenyl monosaccharide derivatives

1980 ◽  
Vol 58 (12) ◽  
pp. 1188-1197 ◽  
Author(s):  
Michael J. Adam ◽  
Laurance D. Hall
Keyword(s):  
Group Technology ◽  
Chemical Shifts ◽  
Soluble Sugar ◽  
Free Water ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Water Soluble ◽  
Coupling Constants ◽  
Spin Lattice Relaxation ◽  
Spin Lattice

Suitably blocked carbohydrates containing free thio 4, amino 5, and hydroxyl 10, 19 nucleophilic functionalities have been treated with (a) ferrocenecarbonyl chlorides 2 and 3, (b) N,N-dimethylaminomethylferrocene methiodide 12, and (c) ferrocenyl tosylate 16 to form the ferrocenyl sugar derivatives 6, 7, 8, 9, 11, 13, 14, 15, and 20. Direct conjugation of a free, water-soluble sugar to ferrocene was achieved by the formation of a Schiff's base between glucosamine hydrochloride 25 and ferrocene carboxaldehyde 24. The synthesis of ferrocenyl sugars using bridging group technology is exemplified by the conjugation of the thio sugar 4 to ferrocene via the versatile coupling reagent cyanuric chloride 21 to form the s-triazine compound 23. These products have been studied by 1H nmr spectroscopy: chemical shifts, coupling constants, and proton spin–lattice relaxation measurements.

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