scholarly journals Co-metabolic Effect of Glucose on Methane Production and Phenanthrene Removal in an Enriched Phenanthrene-Degrading Consortium Under Methanogenesis

2021 ◽  
Vol 12 ◽  
Author(s):  
Ziyan Zhou ◽  
Yanqin Wang ◽  
Mingxia Wang ◽  
Zhifeng Zhou
Keyword(s):  
Anaerobic Digestion ◽  
Removal Rate ◽  
Archaeal Community ◽  
Glucose Addition ◽  
Refractory Compounds ◽  
Α Diversity ◽  
Polycyclic Aromatic ◽  
Degrading System ◽  
Effect Of Glucose ◽  
Multistage Pattern

Anaerobic digestion is used to treat diverse waste classes, and polycyclic aromatic hydrocarbons (PAHs) are a class of refractory compounds that common in wastes treated using anaerobic digestion. In this study, a microbial consortium with the ability to degrade phenanthrene under methanogenesis was enriched from paddy soil to investigate the cometabolic effect of glucose on methane (CH4) production and phenanthrene (a representative PAH) degradation under methanogenic conditions. The addition of glucose enhanced the CH4 production rate (from 0.37 to 2.25mg⋅L−1⋅d−1) but had no influence on the degradation rate of phenanthrene. Moreover, glucose addition significantly decreased the microbial α-diversity (from 2.59 to 1.30) of the enriched consortium but showed no significant effect on the microbial community (R2=0.39, p=0.10), archaeal community (R2=0.48, p=0.10), or functional profile (R2=0.48, p=0.10). The relative abundance of genes involved in the degradation of aromatic compounds showed a decreasing tendency with the addition of glucose, whereas that of genes related to CH4 synthesis was not affected. Additionally, the abundance of genes related to the acetate pathway was the highest among the four types of CH4 synthesis pathways detected in the enriched consortium, which averagely accounted for 48.24% of the total CH4 synthesis pathway, indicating that the acetate pathway is dominant in this phenanthrene-degrading system during methanogenesis. Our results reveal that achieving an ideal effect is diffcult via co-metabolism in a single-stage digestion system of PAH under methanogenesis; thus, other anaerobic systems with higher PAH removal efficiency should be combined with methanogenic digestion, assembling a multistage pattern to enhance the PAH removal rate and CH4 production in anaerobic digestion.

scholarly journals Evidence for PAH Removal Coupled to the First Steps of Anaerobic Digestion in Sewage Sludge

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Glenda Cea-Barcia ◽  
Hélène Carrère ◽  
Jean Philippe Steyer ◽  
Dominique Patureau
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Anaerobic Digestion ◽  
Aromatic Hydrocarbons ◽  
Removal Rate ◽  
Batch Reactors ◽  
Organic Micropollutants ◽  
Hydrophobic Compounds ◽  
Secondary Sludge ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic

Anaerobic degradation of polycyclic aromatic hydrocarbons has been brought to the fore, but information on removal kinetics and anaerobic degrading bacteria is still lacking. In order to explore the organic micropollutants removal kinetics under anaerobic conditions in regard to the methane production kinetics, the removal rate of 12 polycyclic aromatic hydrocarbons was measured in two anaerobic batch reactors series fed with a highly loaded secondary sludge as growth substrate. The results underscore that organic micropollutants removal is coupled to the initial stages of anaerobic digestion (acidogenesis and acetogenesis). In addition, the organic micropollutants kinetics suggest that the main removal mechanisms of these hydrophobic compounds are biodegradation and/or sequestration depending on the compounds.

Microbial Properties and Degradation of Polycyclic Aromatic Hydrocarbons in the Contaminated Farmland Soil with Bioremediation

2014 ◽  
Vol 665 ◽  
pp. 534-537
Author(s):  
Hong Wang ◽  
Hai Bo Li ◽  
Xin Wang ◽  
Ji Fu Ma
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soil ◽  
Significant Positive Correlation ◽  
Removal Rate ◽  
Microbial Properties ◽  
Total Pahs ◽  
Farmland Soil ◽  
Polycyclic Aromatic ◽  
Microbial Agent

Degradation of polycyclic aromatic hydrocarbons (PAHs) and microbial quantity were investigated in aged PAHs-contaminated soil in a pot experiment with regrass and alfalfa. After 60 days germination the concentration of total PAHs in soil decreased by 37.57% and 38.41% with the treatment of ryegrass-microbe agent and alfalfa-microbe agent. The processes of ryegrass and alfalfa were 18.72% and 19.34%. The root system promoted the quantity of microbe and the microbial agent was benefit for the PAHs degrading microbe. And there was significant positive correlation between the number of PAHs degrading microorganisms and the removal rate of PAHs in the soil.

Biodegradation of PAH and DEHP micro-pollutants in mesophilic and thermophilic anaerobic sewage sludge digestion

2006 ◽  
Vol 53 (8) ◽  
pp. 99-107 ◽  
Author(s):  
T. Benabdallah El-Hadj ◽  
J. Dosta ◽  
J. Mata-Álvarez
Keyword(s):  
Anaerobic Digestion ◽  
Wastewater Treatment Plants ◽  
Economic Cost ◽  
Process Efficiency ◽  
Sludge Digestion ◽  
Thermophilic Conditions ◽  
Polycyclic Aromatic ◽  
Positive Effect ◽  
Process Product ◽  
Micro Pollutants

Anaerobic digestion for the treatment of sludge in wastewater treatment plants has been reported to produce a low organic loaded effluent with an acceptable economic cost. But in the last years, new regulations and the increasing sludge production invite us to find an alternative and/or to improve the process efficiency. Moreover, the use of the effluent as fertilizer in agriculture imposes more restrictions on digestion process product and its micropollutant contents to protect the environment. In this study, a performance of the anaerobic digestion under mesophilic and thermophilic conditions at different hydraulic retention times (HRT) is assessed and the removal efficiencies of two important compounds or family compounds (Polycyclic Aromatic Hydrocarbons, PAH, and Di-2-(Ethyl-Hexyl)-Phthalate, DEHP) are evaluated. A positive effect of thermophilic temperature was observed on both micropollutants' biodegradation. However, HRT effect also had an important role for DEHP and low molecular weighted PAH removal.

Anaerobic digestion of waste activated sludge combined with ozone post-treatment and recycling

2003 ◽  
Vol 48 (4) ◽  
pp. 61-68 ◽  
Author(s):  
A. Battimelli ◽  
C. Millet ◽  
J.P. Delgenès ◽  
R. Moletta
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Removal Rate ◽  
Operating Conditions ◽  
Cod Removal ◽  
Process Efficiency ◽  
Recycling Rate ◽  
Waste Activated Sludge ◽  
Digested Sludge ◽  
Combined Process

The aim of the study was to determine the performances of a combined ozone/anaerobic digestion system for waste activated sludge reduction. The objective was the estimation of the process efficiency and stability when keeping constant influent flow while increasing recycled chemically treated flow. The ozonation step consisted in a partial oxidation (0.16 g O3/g SS) of the anaerobic mesophilic digested sludge. Chemical treatment of digested sludge resulted in a threefold COD solubilization and a decrease of SS of 22%. Some of the advantages of digested sludge ozonation were: deodorization, better settlement and a reduction in viscosity. However there were drawbacks: foaming during ozonation and, at high ozone doses, poorer filterability. The anaerobic digestion was carried out over 6 months with an increasing recycling of ozonated flow. Suspended solids removal rate and COD removal rate were compared with initial operating conditions for the biological reactor and the whole combined process. The optimum recycling rate was 25% with increases of SS removal and COD removal of 54% and 66% respectively when considering the combined process; corresponding to a decrease of the hydraulic retention time from 24 days to 19 days.

scholarly journals Effect of glucose on 7-hydroxycoumarin glucuronide production in periportal and pericentral regions of the liver lobule

1985 ◽  
Vol 226 (3) ◽  
pp. 749-756 ◽  
Author(s):  
J G Conway ◽  
F C Kauffman ◽  
R G Thurman
Keyword(s):  
Glucuronic Acid ◽  
Half Time ◽  
Rapid Synthesis ◽  
Fluorescence Excitation ◽  
Glucose Addition ◽  
Liver Lobule ◽  
Tissue Surface ◽  
The Difference ◽  
Light Guides ◽  
Effect Of Glucose

The effect of starvation and glucose addition on glucuronidation was assessed in sublobular regions of the lobule in perfused livers from phenobarbital-treated rats. Fibre-optic micro-light guides were placed on periportal and pericentral areas on the surface of livers to monitor the fluorescence (excitation 366 nm, emission 450 nm) of free 7-hydroxycoumarin from the tissue surface. After infusion of 7-hydroxycoumarin (80 microM) under normoxic conditions, steady-state increases in fluorescence were reached in 6-8 min in both regions. Subsequently, the formation of non-fluorescent 7-hydroxycoumarin glucuronide was inhibited completely by perfusion with N2-saturated perfusate containing 20 mM-ethanol. The difference in fluorescence between anoxic and normoxic perfusions was due to glucuronidation under these conditions. In livers from fed rats, rates of glucuronidation in periportal and pericentral regions of the liver lobule were 8 and 19 mumol/h per g, respectively. In contrast, rates of glucuronidation were 3 and 9 mumol/h per g, respectively, in periportal and pericentral regions of livers from starved rats. Infusion of glucose (20 mM) had no effect on rates of glucuronidation in livers from fed rats; however, glucose increased rates of glucuronidation rapidly (half-time, t0.5 = 1.5 min) in periportal and pericentral regions to 7 and 17 mumol/h per g, respectively in livers from starved rats. These results indicate that the rapid synthesis of the cofactor UDP-glucuronic acid derived from glucose is an important rate-determinant for glucuronidation of 7-hydroxycoumarin in both periportal and pericentral regions of livers from starved rats.

scholarly journals Assessing the Self-Purification Capacity of Surface Waters in Lake Baikal Watershed

Water ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1505 ◽  
Author(s):  
Mikhail Yu. Semenov ◽  
Yuri M. Semenov ◽  
Anton V. Silaev ◽  
Larisa A. Begunova
Keyword(s):  
Lake Baikal ◽  
Water Body ◽  
Removal Rate ◽  
Water Discharge ◽  
Contaminant Removal ◽  
Removal Rates ◽  
Selenga River ◽  
Removal Capacity ◽  
Polycyclic Aromatic ◽  
The Difference

The removal of trace metals (TM), dissolved organic carbon (DOC), mineral nitrogen (Nmin.), and polycyclic aromatic hydrocarbons (PAHs) from the water of Lake Baikal and its tributaries was evaluated. The contaminant removal rate (CRR) and the contaminant removal capacity (CRC) were used as water self-purification parameters. The CRR was calculated as the difference between contaminant mass flow rates at downstream and upstream gauging stations. The CRC was calculated as the quotient of the CRR and the change in water discharge between downstream and upstream gauging stations. Whether the CRR and CRC have positive or negative values depends on whether contaminant release or removal occurs in the water body. The CRR depends on the size of the water body. The lowest and the highest CRRs observed for Baikal were equal to −15 mg/s (PAHs) to −7327 g/s (DOC), whereas the highest PAH and DOC removal rates observed for Selenga River (the major Baikal tributary) in summer were equal to −9 mg/s and −3190 g/s correspondingly. The highest PAH and DOC removal rates observed for small tributaries were equal to 0.0004 mg/s and −0.7 g/s respectively. The amplitude of annual CRR oscillations depends on contaminant abundance. The highest amplitude was typical for most abundant contaminants such as Nmin. and DOC. In unpolluted sections of the Selenga River the highest rates of N and C removal (−85 g/s and −3190 g/s, respectively) were observed in summer and the lowest rates (4 g/s and 3869 g/s, respectively) were observed in the spring. The lowest amplitude was typical for PAHs and some low-abundance TM such as V and Ni. The highest summer rates of V and Ni removal were equal to −378 mg/s and −155 mg/s respectively, whereas lowest spring rates are equal to 296 mg/s and 220 mg/s. The intermediate CRR amplitudes were typical for most abundant TM such as Sr, Al, and Fe. The spatial CRR variability depends on water chemistry and the presence of pollution sources. The lowest (up to 38 g/s) rates of Nmin. removal was observed for polluted lower Selenga sections characterized by low water mineralization and high DOC concentrations. The highest rates (−85 g/s) were observed for unpolluted upper sections. Seepage loss from the river to groundwater was also recognized as an important means of contaminant removal. The CRC values depend mostly on water residence time. The DOC removing capacity value of Baikal (−26 g/m3) were lower than those of Selenga in summer (−35 g/m3) but higher than the CRCs of all tributaries during the other seasons (from 30 mg/m3 to −10 g/m3).

Comparison of changes in selected polycyclic aromatic hydrocarbons concentrations during the composting and anaerobic digestion processes of municipal waste and sewage sludge mixtures

2014 ◽  
Vol 70 (10) ◽  
pp. 1617-1624 ◽  
Author(s):  
Izabela Siebielska
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Aromatic Hydrocarbons ◽  
Degradation Kinetics ◽  
Municipal Waste ◽  
Gas Chromatography Mass Spectrometry ◽  
Organic Fraction ◽  
Important Conclusion ◽  
Polycyclic Aromatic

Changes in naphthalene (Naph), phenanthrene (Phe), pyrene (Pyr), benzo(a)pyrene (BaP) and benzo(ghi)perylene (BgP) concentrations in a mixture of sewage sludge and the organic fraction of municipal waste were determined during composting and anaerobic digestion. The processes were carried out on a laboratory scale. The selected polycyclic aromatic hydrocarbons (PAHs) were analyzed in the samples using gas chromatography-mass spectrometry. The rates at which the PAHs concentrations decreased were evaluated during composting and anaerobic digestion. The selected PAHs degradation kinetics were compared during these processes. The most important conclusion of this work is that composting is much more effective than anaerobic digestion in removing five PAHs from a mixture of sewage sludge and the organic fraction of municipal waste.

Two-Phase Mesophilic Anaerobic Co-Digestion of Food Waste and Sewage Sludge: Effect of Hydraulic Retention Time

2014 ◽  
Vol 852 ◽  
pp. 789-796 ◽  
Author(s):  
Guo Hua Wang ◽  
Lei Wang ◽  
Xue Jun Tan ◽  
Yi Xian Wang ◽  
Feng Wang
Keyword(s):  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Food Waste ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Removal Rate ◽  
Two Phase ◽  
Acidogenic Reactor ◽  
In Series ◽  
The Impact

The impact of hydraulic retention time (HRT) on two-phase mesophilic (35°C) anaerobic co-digestion of food waste and sewage sludge was studied under mixing ratio of 1:1 on the TS basis. Laboratory-scale, two-phase anaerobic digestion systems were employed with each system consisting of an acidogenic reactor and a methanogenic reactor linked in series. For the acidogenic phase, an increase of volatile fatty acid (VFA) concentration was observed as HRT increased from 1d to 5d and the HRT of 5d was recommended for significantly higher VFA production and less propionate percentage, which could provide stable and favourable substrates for the methane reactor. Under acidogenic HRT of 5d, 20d was proved to be the optimum HRT for methanogenic phase with the methane content, methane production rate, methane yield and two-phase VS removal rate reached 71%, 0.7L/(L·d), 0.69L/gVSremoved and 64.7%, respectively. Results verified that the constraints of conventional anaerobic digestion for food waste or sewage sludge separately could be overcome by synergistic effect of co-digestion strategy and two-phase treatment.

scholarly journals Assessment of Nutrients Recovery Capacity and Biomass Growth of Four Microalgae Species in Anaerobic Digestion Effluent

Water ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 221
Author(s):  
Paraskevi Psachoulia ◽  
Sofia-Natalia Schortsianiti ◽  
Urania Lortou ◽  
Spyros Gkelis ◽  
Christos Chatzidoukas ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Biomass Production ◽  
Removal Rate ◽  
Assimilation Efficiency ◽  
Nitrogen Sources ◽  
Ammonium Nitrogen ◽  
Nutrient Assimilation ◽  
Recovery Capacity ◽  
Desmodesmus Communis ◽  
Microalgae Growth

Four microalgae species were evaluated for their bioremediation capacity of anaerobic digestion effluent (ADE) rich in ammonium nitrogen, derived from a biogas plant. Chlorella vulgaris, Chlorella sorokiniana, Desmodesmus communis and Stichococcus sp. were examined for their nutrient assimilation efficiency, biomass production and composition through their cultivation in 3.7% v/v ADE; their performance was compared with standard cultivation media which consisted in different nitrogen sources, i.e., BG-11NO3 and BG-11ΝΗ4 where N-NO3 was replaced by N-NH4. The results justified ammonium as the most preferable source of nitrogen for microalgae growth. Although Stichococcus sp. outperformed the other 3 species in N-NH4 removal efficiency both in BG-11NH4 and in 3.7% ADE (reaching up to 90.79% and 69.69% respectively), it exhibited a moderate biomass production when it was cultivated in diluted ADE corresponding to 0.59 g/L, compared to 0.89 g/L recorded by C. vulgaris and 0.7 g/L by C. sorokiniana and D. communis. Phosphorus contained in the effluent and in the control media was successfully consumed by all of the species, although its removal rate was found to be affected by the type of nitrogen source used and the particular microalgae species. The use of ADE as cultivation medium resulted in a significant increase in carbohydrates content in all investigated species.

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