Development of Carrot Nutraceutical Products as an Alternative Supplement for the Prevention of Nutritional Diseases

Nutraceuticals can serve as an alternative supplement to overcome nutritional deficiency for a healthy lifestyle. They can also play a key role in disease management. To develop carrot nutraceutical products, 64 genotypes from four different continents were evaluated for a range of morpho-nutrition variables. Genetic variability, heritability, strength and direction of association among variables, and direct and indirect relationships among physiochemical and nutritional traits with β-carotene content were evaluated. Core diameter, foliage weight, root weight and shoulder weight showed significant association with β-carotene accumulation. Principal component analysis for physiochemical and nutritional assessment divided these genotypes into two distinctive groups, Eastern carrots and Western carrots. Caloric and moisture content had high positive associations with β-carotene content while carbohydrate content was negatively associated. Five genotypes (T-29, PI 634658, PI 288765, PI 164798, and Ames 25043) with the highest β-carotene contents were selected for making three nutraceutical supplements (carrot-orange juice, carrot jam and carrot candies). These nutraceutical supplements retained high β-carotene content coupled with antioxidant properties. Carrot jam (6.5 mg/100 g) and carrot candies (4.8 mg/100 g) had greater concentrations of β-carotene than carrot-orange juice (1.017 mg/100 g). Carrot jam presented high antioxidant activity with the highest values in T-29 (39% inhibition of oxidation) followed by PI 634658 (37%), PI 164798 (36.5%), Ames 25043 (36%) and PI 288765 (35.5%). These nutraceutical products, with 4–6.5 mg/100 g β-carotene content, had higher values than the USDA recommended dietary intake of 3–6 mg β-carotene/day can be recommended for daily use to lower the risk of chronic disease.

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Antioxidant properties of all-trans-β-carotene/ α-tocopherol mixtures in the induced oxidation of ethyl linoleate

Journal de Chimie Physique ◽

10.1051/jcp/1996931462 ◽

1996 ◽

Vol 93 ◽

pp. 1462-1471

Author(s):

D Perrin ◽

H Eloualja ◽

R Martin

Keyword(s):

Antioxidant Properties ◽

Ethyl Linoleate ◽

Β Carotene

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Intensive Cultivation of Dunaliella salina for Production of Biomass with Elevated β-carotene Content. Communication 1. Effect of Cultivation Factors

Hydrobiological Journal ◽

10.1615/hydrobj.v51.i3.50 ◽

2015 ◽

Vol 51 (3) ◽

pp. 69-76

Author(s):

A. V. Borovkov ◽

I. N. Gudvilovich

Keyword(s):

Dunaliella Salina ◽

Carotene Content ◽

Β Carotene

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Mass Media Influence on Changing Healthy Lifestyle of Community People During COVID-19 Pandemic in Bangladesh: A Cross-Sectional Survey

Asia Pacific Journal of Public Health ◽

10.1177/10105395211011030 ◽

2021 ◽

pp. 101053952110110

Author(s):

Md. Nazrul Islam ◽

Abu Reza Md. Towfiqul Islam ◽

Md. Sajjat Hossain ◽

Md. Tabiur Rahman Prodhan ◽

Mohammad Hasan Chowdhury ◽

...

Keyword(s):

Mass Media ◽

Healthy Lifestyle ◽

Principal Component ◽

Multiple Regression Model ◽

Stepwise Multiple Regression ◽

Media Influence ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Mass Media Influence ◽

Stepwise Multiple Regression Model

We aimed to assess mass media influence on changing the healthy lifestyle behavior of people during the early phase of the COVID-19 (coronavirus disease 2019) pandemic. Principal component analysis and stepwise multiple regression model showed that knowledge level, media credibility, and media check-in had the most considerable contribution to influencing community people’s healthy lifestyle.

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Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽

10.3390/antiox10071081 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1081

Author(s):

Matilda Rădulescu ◽

Călin Jianu ◽

Alexandra Teodora Lukinich-Gruia ◽

Marius Mioc ◽

Alexandra Mioc ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

In Silico ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Melissa Officinalis ◽

Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

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Relationships Linking the Colour and Elemental Concentrations of Blossom Honeys with Their Antioxidant Activity: A Chemometric Approach

Agriculture ◽

10.3390/agriculture11080702 ◽

2021 ◽

Vol 11 (8) ◽

pp. 702

Author(s):

Monika Kędzierska-Matysek ◽

Anna Teter ◽

Małgorzata Stryjecka ◽

Piotr Skałecki ◽

Piotr Domaradzki ◽

...

Keyword(s):

Antioxidant Activity ◽

Physicochemical Properties ◽

Black Locust ◽

Antioxidant Properties ◽

Principal Component ◽

Botanical Origin ◽

Antioxidant Parameters ◽

Colour Parameters ◽

Hue Angle ◽

Chemometric Approach

The antioxidant activity of honey depends on the botanical origin, which also determines their physicochemical properties. In this study, a multivariate analysis was used to confirm potential relationships between the antioxidant properties and colour parameters, as well as the content of seven elements in five types of artisanal honey (rapeseed, buckwheat, linden, black locust, and multifloral). The type of honey was found to significantly influence most of its physicochemical properties, colour parameters, and the content of potassium, manganese and copper. Antioxidant parameters were shown to be significantly positively correlated with redness and concentrations of copper and manganese, but negatively correlated with the hue angle and lightness. The principal component analysis confirmed that the darkest buckwheat honey had the highest antioxidant activity in combination with its specific colour parameters and content of antioxidant minerals (manganese, copper and zinc). The level of these parameters can be potentially used for the identification of buckwheat honey.

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Intersexual Partial Diploids of Phycomyces

Genetics ◽

10.1093/genetics/158.2.635 ◽

2001 ◽

Vol 158 (2) ◽

pp. 635-641

Author(s):

Bina J Mehta ◽

Enrique Cerdá-Olmedo

Keyword(s):

Dry Mass ◽

Sexual Cycle ◽

Genetic Constitution ◽

Phycomyces Blakesleeanus ◽

Carotene Content ◽

Sexual Interaction ◽

Sexual Stimulation ◽

Nuclear Composition ◽

Opposite Sex ◽

Β Carotene

Abstract Sexual interaction between strains of opposite sex in many fungi of the order Mucorales modifies hyphal morphology and increases the carotene content. The progeny of crosses of Phycomyces blakesleeanus usually include a small proportion of anomalous segregants that show these signs of sexual stimulation without a partner. We have analyzed the genetic constitution of such segregants from crosses that involved a carF mutation for overaccumulation of β-carotene and other markers. The new strains were diploids or partial diploids heterozygous for the sex markers. Diploidy was unknown in this fungus and in the Zygomycetes. Random chromosome losses during the vegetative growth of the diploid led to heterokaryosis in the coenocytic mycelia and eventually to sectors of various tints and mating behavior. The changes in the nuclear composition of the mycelia could be followed by selecting for individual nuclei. The results impose a reinterpretation of the sexual cycle of Phycomyces. Some of the intersexual strains that carried the carF mutation contained 25 mg β-carotene per gram of dry mass and were sufficiently stable for practical use in carotene production.

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Preliminary Study on Pasta Samples Characterized in Antioxidant Compounds and Their Biological Activity on Kidney Cells

Nutrients ◽

10.3390/nu13041131 ◽

2021 ◽

Vol 13 (4) ◽

pp. 1131 ◽

Cited By ~ 1

Author(s):

Federico Di Marco ◽

Francesco Trevisani ◽

Pamela Vignolini ◽

Silvia Urciuoli ◽

Andrea Salonia ◽

...

Keyword(s):

Indoleacetic Acid ◽

Antioxidant Properties ◽

Antioxidant Compounds ◽

Kidney Cells ◽

Hek 293 ◽

293 Cells ◽

High Concentrations ◽

Β Carotene ◽

Egg Pasta

Pasta is one of the basic foods of the Mediterranean diet and for this reason it was chosen for this study to evaluate its antioxidant properties. Three types of pasta were selected: buckwheat, rye and egg pasta. Qualitative–quantitative characterization analyses were carried out by HPLC-DAD to identify antioxidant compounds. The data showed the presence of carotenoids such as lutein and polyphenols such as indoleacetic acid, (carotenoids from 0.08 to 0.16 mg/100 g, polyphenols from 3.7 to 7.4 mg/100 g). To assess the effect of the detected metabolites, in vitro experimentation was carried out on kidney cells models: HEK-293 and MDCK. Standards of β-carotene, indoleacetic acid and caffeic acid, hydroalcoholic and carotenoid-enriched extracts from samples of pasta were tested in presence of antioxidant agent to determine viability variations. β-carotene and indoleacetic acid standards exerted a protective effect on HEK-293 cells while no effect was detected on MDCK. The concentrations tested are likely in the range of those reached in body after the consumption of a standard pasta meal. Carotenoid-enriched extracts and hydroalcoholic extracts showed different effects, observing rescues for rye pasta hydroalcoholic extract and buckwheat pasta carotenoid-enriched extract, while egg pasta showed milder dose depending effects assuming pro-oxidant behavior at high concentrations. The preliminary results suggest behaviors to be traced back to the whole phytocomplexes respect to single molecules and need further investigations.

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Dietary Data in the Malmö Offspring Study–Reproducibility, Method Comparison and Validation against Objective Biomarkers

Nutrients ◽

10.3390/nu13051579 ◽

2021 ◽

Vol 13 (5) ◽

pp. 1579

Author(s):

Sophie Hellstrand ◽

Filip Ottosson ◽

Einar Smith ◽

Louise Brunkwall ◽

Stina Ramne ◽

...

Keyword(s):

Fruits And Vegetables ◽

Method Comparison ◽

Principal Component ◽

Fish Intake ◽

Dietary Intakes ◽

Food Groups ◽

Food Record ◽

Fatty Fish ◽

Lean Fish ◽

Β Carotene

Irregular dietary intakes impairs estimations from food records. Biomarkers and method combinations can be used to improve estimates. Our aim was to examine reproducibility from two assessment methods, compare them, and validate intakes against objective biomarkers. We used the Malmö Offspring Study (55% women, 18–71 y) with data from a 4-day food record (4DFR) and a short food frequency questionnaire (SFFQ) to compare (1) repeated intakes (n = 180), (2) intakes from 4DFR and SFFQ (n = 1601), and (3) intakes of fatty fish, fruits and vegetables, and citrus with plasma biomarkers (n = 1433) (3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid [CMPF], β-carotene and proline betaine). We also combined 4DFR and SFFQ estimates using principal component analysis (PCA). Moderate correlations were seen between repeated intakes (4DFR median ρ = 0.41, SFFQ median ρ = 0.59) although lower for specific 4DFR-items, especially fatty/lean fish (ρ ≤ 0.08). Between-method correlations (median ρ = 0.33) were higher for intakes of overall food groups compared to specific foods. PCA scores for citrus (proline betaine ρ = 0.53) and fruits and vegetables (β-carotene: ρ = 0.39) showed the highest biomarker correlations, whereas fatty fish intake from the SFFQ per se showed the highest correlation with CMPF (ρ = 0.46). To conclude, the reproducibility of SFFQ data was superior to 4DFR data regarding irregularly consumed foods. Method combination could slightly improve fruit and vegetable estimates, whereas SFFQ data gave most valid fatty fish intake.

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A study on the vitamin A activity of carotene in green fodder

The Journal of Agricultural Science ◽

10.1017/s002185960001162x ◽

1946 ◽

Vol 36 (2) ◽

pp. 95-99 ◽

Cited By ~ 1

Author(s):

B. C. Ray Sarkar ◽

K. C. Sen

Keyword(s):

Vitamin A ◽

Relative Efficiency ◽

Chemical Method ◽

Plant Tissues ◽

Carotene Content ◽

A Value ◽

Green Fodder ◽

Biological Tests ◽

Different Sources ◽

Β Carotene

1. With the object of determining the vitamin A value of carotene in different green fodders, an investigation has been undertaken to study (i) the relation between the chemically determined carotene and its biological activity as compared with that of standard carotene, (ii) the purity of apparent carotene from different sources, (iii) absorption of carotene in rats, and (iv) the relative efficiency of the standard carotene and preformed vitamin A.2. Biological tests have shown that the chemical method of assay is a fair index of the true carotene content in green fodders, and carotene in the form of an extract is quite as effective in the system as that present in the plant tissues. β-Carotene appears to be predominant in these materials.

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