scholarly journals Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles

2021 ◽  
Vol 12 ◽  
Author(s):  
Elżbieta Kondera ◽  
Bartosz Bojarski ◽  
Katarzyna Ługowska ◽  
Barbara Kot ◽  
Małgorzata Witeska
Keyword(s):  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Leukocyte Count ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Hematopoietic Tissue ◽  
Differential Leukocyte Count ◽  
Thyme Oil

The effects of two potential antibacterial agents of plant origin: trans-cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of Cyprinus carpio were studied. Both phytochemicals were used in the doses based on the bactericidal concentrations against Aeromonas spp. developed earlier in in vitro study. The fish were fed for 2 weeks on a commercial feed supplemented with 10 μl/kg of TC or 20 μl/kg of TO. Groups TC1 and TO1 were fed diets containing phytochemicals daily, while groups TC2 and TO2 every 2 days. Control group and groups TC2 and TO2 on the remaining days were fed plain feed. Peripheral blood and head kidney hematopoietic tissue were sampled from all the fish at the end of the experiment. In all the groups, hematological values were within the reference ranges for the healthy common carp juveniles. However, blood hemoglobin (Hb) concentration and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in all the groups exposed to TC and TO, while MCH in TC1, TO1, and TO2 compared to the control. TC and TO did not affect leukocyte count [white blood cell (WBC)], differential leukocyte count, the oxidative activity of phagocytes [nitroblue tetrazolium (NBT)], or thrombocyte count (Thro). No significant alterations were observed in the hematopoietic tissue. The results showed that TC and TO exhibited no considerable hematotoxic effects and trials of their use in the treatment of fish infected with Aeromonas spp. may be undertaken.

scholarly journals Evaluation of the Effect of Hydroalcoholic Extracts of Cassia Occidentalis Leaves in Neutrophil Adhesion Test in Rats

2020 ◽  
Vol 9 (4-s) ◽  
pp. 1218-1221 ◽  
Author(s):  
Dhaval M Patel ◽  
Advaita B Patel ◽  
Riddhi D Trivedi ◽  
Viram J Parmar ◽  
Urvisha V Bangoriya
Keyword(s):  
Oral Administration ◽  
Leukocyte Count ◽  
Control Group ◽  
Neutrophil Adhesion ◽  
Adhesion Test ◽  
Differential Leukocyte Count ◽  
Hydroalcoholic Extract ◽  
Cassia Occidentalis ◽  
Blood Samples ◽  
Blood Smears

Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis leaves in neutrophil adhesion test in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis leaves on neutrophil has been studied and is compared with control group on rats. The differential leukocyte count (DLC) was performed by fixing the blood smears and staining with leucofine and percent neutrophils in each sample was determined. After the initial counts, blood samples were incubated with 80 mg/ml of nylon fibers for 10 min at 37ºC. Supplementation with hydroalcoholic extract of Cassia occidentalis leaves significantly decreased neutrophil adhesion. The results indicate that the leaf of Cassia occidentalis is endowed with protected neutrophil adhesion. These effects could conclude that Cassia occidentalis has an antiasthmatic property. Keywords: Cassia occidentalis, Neutrophil adhesion, analysis of variance

scholarly journals Immunostimulatory properties of bigroot geranium (Geranium macrorrhizum L.) extract

Medicina ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 60 ◽  
Author(s):  
Vilma Jurkštienė ◽  
Anatolijus Kondrotas ◽  
Egidijus Kėvelaitis
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Food Supplement ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Case Group ◽  
Cell Counts

The aim of the study was to investigate the immunostimulatory properties of bigroot geranium. Material and methods. Possible nonspecific characteristics of bigroot geranium were evaluated by the total leukocyte count in the peripheral blood, and qualitative changes of blood were assessed using Shilling’s formula by evaluating changes in lymphocyte counts. In addition, we also studied changes in the counts of Tcell precursors in the thymus and B lymphocytes in the spleen. Ethanol extract of the leaves of bigroot geranium was produced at the Department of Food Technology, Kaunas University of Technology. Studies were performed on mice Bl 57 (n=21). The control group (n=7) received distilled water at a dose of 1 mL/day. The second and third groups received 1% and 10% extract of bigroot geranium, respectively, as a food supplement. Changes in cell counts were investigated after 4 weeks following the initiation of the trial. Results. After a 4-week administration of 1% extract of bigroot geranium (1 mL/day) (mice group, n=7), leukocyte count in the peripheral blood increased to 6.1×109 cells/L, and lymphocyte count – to 70%, but changes were not statistically significant. The other case group of mice (n=7) received 10% extract of bigroot geranium for 4 weeks at a dose of 1 mL/day. In this group, leukocyte count in the peripheral blood increased statistically significantly from 4.4×109 cells/L to 7.2×109 cells/L (p<0.01), and lymphocyte percentage – from 52% to 80% (p<0.001), as compared to control. Thymocyte (T lymphocytes) counts in thymus and splenocyte (B lymphocytes) counts in the spleen showed a tendency to increase after the administration of 1% and 10% extracts. After a 4-week administration of 1% extract of bigroot geranium, thymocyte and splenocyte counts increased from 0.342×106 cells to 0.372×106 cells per mg of tissue and from 0.395×106 cells to 0.405×106 cells per mg of tissue, respectively, as compared to control group (p>0.1). After the administration of 10% extract of bigroot geranium, thymocyte count increased to 0.488×106 cells per mg of tissue (p<0.01), and splenocyte count – to 0.504×106 cells per mg of tissue (p<0.01). Conclusion. The extracts of the leaves of bigroot geranium increased leukocyte count and lymphocyte percentage in the peripheral blood, and after a 4-week administration of 10% extract of bigroot geranium, a statistically significant increase in the counts of T lymphocytes (in the thymus) and B lymphocytes (in the spleen) was observed. The immunostimulatory effect depends on the dose of the extract.

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

Downregulation of SATB1 Increase the Invasiveness of Jurkat Cell Via Activation of the WNT/Beta-Catenin Signaling Pathway in Vitro

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4825-4825
Author(s):  
Xiaodan Luo ◽  
Lihua Xu ◽  
Dan Liu ◽  
Yaya Wang ◽  
Xiaohong Wu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Peripheral Blood ◽  
Jurkat Cell ◽  
Cellular Proliferation ◽  
Conflicts Of Interest ◽  
Expression Patterns ◽  
Control Group ◽  
Beta Catenin ◽  
Normal Controls

Abstract Backgroud: Special AT-rich sequence-binding protein-1 (SATB1) is critical for genome organizer that reprograms chromatin organization and transcription profiles, and associated with tumor growth and metastasis in several cancer types. Many studies suggest that SATB1 overexpression is an indicator of poor prognosis in various cancers, such as breast cancer, malignant cutaneous melanoma, liver cancer, etc. However, their expression patterns and function values for adult T-cell leukemia (ATL) are still largely unknown. Objective: The aim of this study is to examine the levels of SATB1 in ATL and to explore its function and mechanisms in ATL. METHODS: 20 ATL peripheral blood samples and 20 normal controls were collected. Expressions of SATB1 in both groups were evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cellular proliferation and invasion of SATB1-knockdown Jurkat cells and cells in control group were evaluated by manually count and transwell matrigel invasion assay, respectively. RESULTS: SATB1 expressions were decreased in ATL peripheral blood mononuclear cells (p<0.001) compared with normal controls. Knockdown of SATB1 gene might increase Jurkat cell invasiveness through the activation of Wnt/β-Catenin signaling pathway. CONCLUSIONS: SATB1 expression is down-regulated in ATL and decreased expression of SATB1 increase the invasiveness of Jurkat cell through the activation of Wnt/β-Catenin signaling pathway in vitro. Acknowledgments This study was supported by grants from the National Natural Science Foundation of China (81200399) and Key Clinical Disciplines of Guangdong Province (20111219) Disclosures No relevant conflicts of interest to declare.

scholarly journals Impact of Black Grape byproducts on Blood Picture in Common Carp (Cyprinus carpio L.)

2017 ◽  
Vol 30 (1) ◽  
pp. 32-37
Author(s):  
Pola J. Nader ◽  
Nasreen M. Abdulrahman
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
White Blood Cells ◽  
Diet Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Fish Farms ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Feeding Trials

This experiment was conducted for 70 days on 165 common carp Cyprinus carpio fingerlings which were brought from local fish farms located in Daqooq, Haftaghar, Al-Tameem province. Fishes were distributed among experimental tanks with mean initial weight of 24.09 gm. They were pre-acclimated to laboratory conditions for 21 days prior to the feeding trials. The experiment was conducted by using 15 plastic tanks (100 L) representing five treatments with three replicates. Each tank was stocked with 11 fishes. Treatments included 0, 2.5, 5.0, 7.5 or 10 gm grape byproduct/ kg diet. The result showed that red blood cell (RBC) data of the control group was significantly higher than other treatments. Hemoglobin of fishes in groups 2.5 and 10 gm/ kg diet was significantly different from other treatments. Mean corpuscular hemoglobin (MCH) values for 0.0, 5.0 and 10 gm/kg groups were significantly different, so mean corpuscular volume (MCV) values showed significant differences resulted from 0.0, 5.0, 7.5 and 10 gm/ kg diet compared to 2.5 gm/ kg diet group. The white blood cells (WBC) were significantly different in 2.5, 5.0 and 7.5 gm/ kg diet. Granulocyte percentages obtained in all treatments were significantly different from control. Lymphocyte percentages were significantly different in all treatments compared to 10 gm/ kg diet group. The Monocytes were significantly different in 2.5, 7.5 and 10 gm/ kg groups.

Peripheral Blood HPC Counts Are Augmented in CML Patients with Cytogenetic Findings of Disease as Compared to Patients in Cytogenetic Remission.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4691-4691
Author(s):  
Ernesto de Meis ◽  
Rita C.B. Morais ◽  
Luize O. Carvalho ◽  
Arthur Moellmann-Coelho ◽  
Maria M.K. Martinez ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Myeloid Leukemia ◽  
Leukocyte Count ◽  
Philadelphia Chromosome ◽  
Cell Disease ◽  
Differential Leukocyte Count ◽  
Non Invasive ◽  
Cd34 Cells ◽  
High Prevalence ◽  
Cytogenetic Remission

Abstract Chronic Myeloid Leukemia (CLM) is a stem cell disease characterized by inhibition of apoptosis and increased proliferation of bone marrow (BM) compounds. Evaluation of hematopoetic precursors cells on the peripheral blood (PB) of these patients show a high prevalence of leukemic CD34+ cells. Recently it was demonstrated that measurement of HPC by IMI channel on Sysmex showed an acceptable correlation between Hematopoetic Progenitors CD34+ cells counts. The aim of this work was to compare precursor cells (HPC) numbers in the PB of CML patients with disease and under remission. For this we studied 61 CML patients that had been treated with different protocols. From these 22 were in cytogenetic remission but 39 still presented more than 5% BM cells with Philadelphia chromosome (Ph+). Although the normal levels of CD34+ HPC in PB is considered to be very low, we considered less than 0.05% of leukocytes as normality. HPC in PB were counted using an automated peripheral blood counter (Sysmex XE-2100). We found that Ph+ CML patients had a significant increased number of PB HPC compared to CML patients under remission (with a confidence interval of 95%, and a corrected Yates analyses with X2 16.78 and p<0.0001). The increase in PB HPC was not related with the number of white cells counts at moment of the exam. These findings suggest that the presence of a high number of PB HPC in CML can be used as a non-invasive marker of the disease, more reliable than total and differential leukocyte count.

In vitro effect of lentinan on the activation of immunological cells in Cyprinus carpio

2009 ◽  
Vol 6 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Zhang Liu ◽  
Cao Li-Ping ◽  
Ding Wei-Dong ◽  
Galina Jeney ◽  
Xu Pao ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Head Kidney ◽  
Burst Activity ◽  
Griess Reaction ◽  
Nitroblue Tetrazolium Reduction ◽  
Diphenyl Tetrazolium Bromide ◽  
Vitro Effect ◽  
Peripheral Blood Leucocytes

AbstractMacrophages from the head kidney (HK) and peripheral blood leucocytes were isolated fromCyprinus carpio by Percoll gradient density centrifugation, cultured in vitro and exposed to different concentrations of the immunomodulator lentinan. To evaluate the immunostimulating effects of lentinan, proliferation of the peripheral blood leucocytes, respiratory burst of macrophages and interleukin-1β (IL-1β) gene mRNA expression of macrophages were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], NBT (nitroblue tetrazolium) reduction, Griess reaction and real-time polymerase chain reaction (PCR). Results showed that proliferation of peripheral blood leucocytes after 24 h incubation by the induction of lentinan at 100 and 1000 μg/ml was markedly stimulated. Lentinan at 1, 100 and 1000 μg/ml could significantly induce superoxide anions in macrophages. Production of nitric oxide by HK macrophages after 96 h incubation by lentinan showed that it had no conspicuous effect on nitrogen burst activity of macrophages. Moreover, it inhibited the nitrogen burst activity at higher doses. The expression of IL-1β in the HK macrophages after 24 h of polysaccharide stimulation showed that lentinan stimulated IL-1β expression in the head kidney in carp. Lentinan can modulate the immune response of C. carpio.

scholarly journals Ageing and production of the cytokines in Chernobyl clean-up workers from Latvia

2009 ◽  
Vol 63 (3) ◽  
pp. 87-92
Author(s):  
Nataļja Kurjāne ◽  
Rūta Brūvere ◽  
Natālija Gabruševa ◽  
Elvīra Hagina ◽  
Tija Zvagule ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Donors ◽  
Age Groups ◽  
Control Group ◽  
Low Grade ◽  
Peripheral Blood Mononuclear ◽  
Age Related ◽  
Good Ability ◽  
Tnf Α

Ageing and production of the cytokines in Chernobyl clean-up workers from Latvia Chronic low-grade inflammation with subsequent impairment of immune system function promotes the development of age-related diseases, such as cancers, degenerative and infection diseases. It is not yet clear, if exposure to ionising radiation accelerates the aging process. The aim of the present work was to estimate the production of several cytokines by peripheral blood cells of Latvia's Chernobyl clean-up workers depending on age. ELISA was employed to determine the plasma level of sIL-1β and sIL-6 as well as level of IL-4 and TNF-α spontaneous and 24h and 96h after in vitro stimulation of peripheral blood mononuclear cell cultures by lipopolysaccharide (LPS) and phytohemagglutinin (PHA) mitogens were determined in 40 Chernobyl clean-up workers 17 years after their work in Chernobyl and in 42 blood-donors without a history of occupational radiation exposure. The ability of peripheral blood leukocytes (PBL) to produce interferons (IFNs) was determined in 73 Chernobyl clean-up workers 15 years after the work in Chernobyl and in age-matched 63 blood-donors. IFNs were tested in whole blood cultures by standard virus cytopathic inhibition micromethod after their in vitro induction by Newcastle disease virus, phytohemagglutinin or double-stranded RNA. Individuals were divided into three age groups: age < 40, age 40-49 and age > 50. The ability of PBL to produce IFN was significantly decreased in all Chernobyl clean-up worker age groups in comparison with blood-donors (control groups). The incidence of good ability to produce IFN gradually decreased with age in the control group, but increased with age in aged-matched Chernobyl clean-up workers groups. The production TNF-α and IL-4 by peripheral blood mononuclear cells as well as sIL-1β level in plasma showed no significant differences between all the examined age groups. The sIL-6 level was gradually increased with age in Chernobyl clean-up workers. Our results showed that the concentration of pro-inflammation cytokine sIL-6 in peripheral blood plasma, as well as the ability of PBL to produce IFNs, in Chernobyl clean-up workers from Latvia is age dependent.

scholarly journals Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats

2021 ◽  
Vol 11 (1-s) ◽  
pp. 117-120
Author(s):  
Dhaval M M Patel ◽  
Advaita B Patel ◽  
Bhumi R Patel ◽  
Riddhi D Trivedi ◽  
Deepa R Patel
Keyword(s):  
Oral Administration ◽  
Leukocyte Count ◽  
Control Group ◽  
Neutrophil Adhesion ◽  
Adhesion Test ◽  
Differential Leukocyte Count ◽  
Hydroalcoholic Extract ◽  
Cassia Occidentalis ◽  
Blood Samples ◽  
Blood Smears

Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis seeds on neutrophil has been studied and is compared with control group on rats. The differential leukocyte count (DLC) was performed by fixing the blood smears and staining with leucofine and percent neutrophils in each sample was determined. After the initial counts, blood samples were incubated with 80 mg/ml of nylon fibers for 10 min at 37ºC. Supplementation with hydroalcoholic extract of Cassia occidentalis seedss significantly decreased neutrophil adhesion. The results indicate that the seed of Cassia occidentalis is endowed with protected neutrophil adhesion. Keywords: Cassia occidentalis, Neutrophil, analysis of variance

scholarly journals Effect of immunocytotherapy on the state of the immune system of women with idiopathic habitual miscarriage

2020 ◽  
Vol 22 (4) ◽  
pp. 751-764
Author(s):  
L. V. Krechetova ◽  
V. V. Vtorushina ◽  
E. V. Inviyaeva ◽  
L. V. Vanko ◽  
M. A. Nikolaeva ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Th17 Cells ◽  
Peripheral Blood Lymphocytes ◽  
Inflammatory Factors ◽  
Control Group ◽  
In Vitro Production ◽  
Th17 Response ◽  
Low Level ◽  
Vitro Production

We aimed  for assessing effects of immunocytotherapy upon  the subpopulations of CD4+CD25highFoxP3+ cellswithnaturalregulatoryactivityandactivatedTh17cellswiththeCD4+CD25highRORγt+ phenotype, as well as in vitro production of cytokines in mitogen-stimulated cells from  peripheral blood  in the patients with idiopathic habitual miscarriage (IHM). The study group consisted of 33 patients with IHM who became pregnant after a pre-gestational alloimmunization. In 27 patients, the pregnancy was prolonged to the  full term  and  ended  with the  birth  of viable babies,  in six cases it was terminated before  12 weeks of gestation. Before  administration of immunocytotherapy (ICT), 19 patients were examined, of them  16 after alloimmunization outside of pregnancy, 17 at 5-6 and 8-9 weeks of pregnancy. Eleven patients were immunized at 12 weeks of pregnancy. In the control group,  12 fertile women  outside  pregnancy and 10 women  at 12 weeks of physiological pregnancy were examined. The proportion of FoxP3+ and RORγt+ cells with the CD4+CD25high phenotype was evaluated among  T-lymphocytes from peripheral blood,  as well as content of proinflammatory cytokines (IFNγ,  TNFα, IL-1β, IL-2, IL-5, IL  -6,  IL-8, IL-12p70) and  anti-inflammatory factors  (IL-4, IL-10), as well as IL-17  amounts.We have found  that,  following pre-gestational alloimmunization, the women  who lost this pregnancy, had a  low  level  of  FoxP3+Тregs that  suppress  pro-inflammatory Th17-dependent  reactions, however, without changing levels of activated Th17  cells (CD4+CD25highRORγt+   lymphocytes). These  facts,  along  with  high in vitro production of IL-17  by peripheral blood cells at the terms of 5-6 weeks of gestation, suggest that,  after pre-gestational alloimmunization in women  with miscarriage, a predilection is formed  to pro-inflammatory cytokine production. However, at the 5-6 week-period, it is realized  not in the Th1 direction of, but towards Th17 response, and a low level of CD4+CD25highRORγt+ cells may reflect an increased migration of Th17 cells from peripheral blood to the uterine endometrium.Thus,  we have shown  the  effect of immunocytotherapy upon  subpopulational composition of peripheral blood  lymphocytes and  the  cytokine profile,  as well as upon  the  course  of first trimester and  outcomes of pregnancy in women  with idiopathic habitual miscarriage.

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