scholarly journals Identification of Vicia Species Native to South Korea Using Molecular and Morphological Characteristics

2021 ◽  
Vol 12 ◽  
Author(s):  
Seahee Han ◽  
Raveendar Sebastin ◽  
XiaoHan Wang ◽  
Kyung Jun Lee ◽  
Gyu-Taek Cho ◽  
...  
Keyword(s):  
South Korea ◽  
Native Species ◽  
Morphological Characteristics ◽  
Intraspecific Variability ◽  
Vital Role ◽  
Forage Crops ◽  
Vicia Villosa ◽  
Interspecific Distance ◽  
Vicia Cracca ◽  
Alignment Analysis

Recently, within the Fabaceae family, the Vicia genus has been recognized for its vital role in sustainable agriculture. Vicia species are economically important grain and forage crops. However, the presence of complex morphological characteristics makes identification and recognition of native species difficult. In this study, the possibility of using DNA barcoding regions (ITS2, matK, and rbcL) to distinguish among 19 Vicia taxa (59 accessions) found in South Korea was evaluated. The sequence alignment analysis revealed considerable nucleotide diversity (π) between the loci, in which ITS2 showed the highest mean interspecific distance, whereas there was no intraspecific variability among the barcode regions in 12 of the 19 taxa. Phylogenetic analysis of combined barcoding regions revealed well-resolved phylogeny with the highest species level discrimination. Combinations of barcode loci were also used in classification at the subgenera and section levels. The results revealed that the combined barcoding regions can be used effectively to differentiate the following species: Vicia angustifolia var. segetilis, Vicia bungei, Vicia villosa, Vicia cracca, Vicia dasycarpa, Vicia hirsuta, Vicia tetrasperma, Vicia amurensis, Vicia hirticalycina, and Vicia chosenensis. However, it is difficult to differentiate the species of Vicia unijuga, Vicia unijuga var. kaussanensis, Vicia linearifolia, Vicia unijuga f. angustifolia, Vicia nipponica, Vicia amoena, Vicia venosa var. cuspidata, Vicia pseudo-orobus, and Vicia japonica with the tested barcode regions. These species come under sect. Vicilla and are found to be closely related or species that have recently undergone speciation; thus, it has limitation to distinguish with recommended barcodes. Hence, to differentiate the unclassified species, 39 morphological characteristics were investigated, in which 16 useful characteristics were selected for efficient classification. Finally, the 16 selected morphological useful traits efficiently differentiated all the Vicia species. In conclusion, a combination of barcoding loci together with morphological characteristics of this study efficiently discriminated all the Korean Vicia species.

scholarly journals New Balkan records of Bombus subterraneus (Linnaeus 1758) and Bombus cryptarum (Fabricius 1775) from Greece

2017 ◽  
Vol 18 ◽  
pp. 56 ◽  
Author(s):  
Ioannis Th. Anagnostopoulos
Keyword(s):  
Mitochondrial Dna ◽  
New Records ◽  
Morphological Characteristics ◽  
Rflp Analysis ◽  
Original Data ◽  
Insect Fauna ◽  
Bumblebee Species ◽  
Species Lists ◽  
Vicia Cracca ◽  
First Time

From the study of the Greek bumblebee fauna (Hymenoptera: Apidae, Bombini), species lists have been published based on both literature records and original data from collected bees. Since 1995 a special effort to confirm with newly collected bees all bumblebee species reported in literature records for Greece has been in progress. Although numerous specimens have been collected and examined and in some instances yielding new Bombus species for the Greek insect fauna, some species, mainly those reported in older references, have not yet been found. Recently, identification of bumblebees collected in the Florina Prefecture - Northwest Macedonia, during the years 2006 and 2007 yielded information for two “literature cited” species, Bombus subterraneus (Linnaeus 1758) and Bombus cryptarum (Fabricius 1775). A B. subterraneus queen (collected at 40°47´38N, 21°26´10E on Vicia cracca) was distinguished by morphological characteristics and a worker B. cryptarum (collected at 40°41´58,7N, 21°28´18,5E on Echium spp) was revealed using mitochondrial DNA RFLP analysis of the CO1 gene. These new records from Florina are provided with comments, confirming the species presence in Greece for the first time after approximately 40 years.

Grasses of the Precambrian Shield region in eastern lowland Bolivia. II. Life-form and C3–C4 photosynthetic types

1992 ◽  
Vol 8 (4) ◽  
pp. 409-433 ◽  
Author(s):  
Timothy J. Killeen ◽  
Paul N. Hinz
Keyword(s):  
Relative Abundance ◽  
Native Species ◽  
Life Form ◽  
Deciduous Forest ◽  
Morphological Characteristics ◽  
Grass Species ◽  
Individual Species ◽  
Campo Rupestre ◽  
Granite Outcrops ◽  
Bunch Grass

ABSTRACTA life-form classification from Neotropical Gramineae is proposed for 113 native species from eastern lowland Bolivia. A factor analysis was performed on a correlation matrix of 51 vegetative attributes to identify ‘character suites,’ which were then used as the basis for the classification. The principal types are arboreal, liana, shrub-like, forb-like, bunch grass with basal foliage, bunch grass with basal and caulescent foliage, caespitose annuals, elongate rhizomes, turf grass, stoloniferous, and decumbent (the last category is further divided into subgroups: -perennial, -indefinite, -floating aquatic, and -vine-like). A key component of the life-form classification is the morphology of leaf blades, with five major patterns recognized: elongate and inrolled, elongate and conduplicate, elongate and flat with an attenuate base, short and broad with an amplexicaule base, and short and broad with a pseudopetiole.The distribution and relative abundance of three major photosynthetic categories were compared with life-form and habitat preference. Photosynthetic type of individual species was identified based on leaf anatomy. C4-(XyMS −) species predominated in cerradao, cerrado, campo rupestre, valleyside campos, and seasonally inundated savanna, as well as on granite outcrops. C4-(XyMS + ) species were relatively rare in all habitats, but were significantly more abundant in the xeric microenvironments of granite outcrops. C3 species predominated in semi-deciduous forest and increased in relative abundance in wetland habitats. Caespitose annuals and bunch grass species were predominantly C4 type, while arboreal and liana species were uniformly C3 type. Grasses classified as decumbent or shrub-like were predominantly C3 type, while rhizomatous, stoloniferous, and forblike grasses were a mixture of C3 and C4 type.The intent of the study was to group morphological characteristics objectively in order to derive a life-form classification specific for the Gramineae. Comparisons of the relative abundance of the various life-forms in a broad range of habitat types provide a descriptive assessment of the adaptive value of these characteristics.

Morphological and molecular characterization of Gambierdiscus caribaeus (Dinophyceae), with a confirmation of its occurrence in the Colombian Caribbean Tayrona National Natural Park

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Edgar Arteaga-Sogamoso ◽  
Francisco Rodríguez ◽  
José Ernesto Mancera-Pineda
Keyword(s):  
Morphological Characteristics ◽  
Intraspecific Variability ◽  
Original Description ◽  
Single Strain ◽  
Natural Park ◽  
Fish Poisoning ◽  
Rdna Sequencing ◽  
Local Species ◽  
Western Side ◽  
A Site

Abstract Dinoflagellates in the genus Gambierdiscus are known to produce potent neurotoxins known as ciguatoxins. These toxins vector through the food web and can reach sufficiently high levels to cause ciguatera fish poisoning (CFP). In many regions of the Caribbean, little is known about the occurrence of CFP-causing species or the incidence of CFP, despite its apparent regional increase over the last few decades. Given the intraspecific variability in content and potencies of these toxins among Gambierdiscus species, the precise identification of local species of this genus is crucial. In this study we confirm the presence of the epibenthic dinoflagellate Gambierdiscus caribaeus in the Colombian continental Caribbean. Cells were collected in seagrass beds of Thalassia testudinum in Bonito Gordo, a site located on the western side of Bahía Concha, Tayrona National Natural Park, near the city of Santa Marta, Colombia. Subsequently, a single strain of Gambierdiscus could be established for further identification. Morphological (light and scanning electron microscopy), and molecular results (partial LSU and ITS rDNA sequencing) were consistent with the original description of G. caribaeus, as well as with the morphological characteristics shown by other authors in organisms obtained near the sampling area.

scholarly journals First Report on Ovate-leaf Atractylodes Damping-off Caused by Fusarium oxysporum species Complex in South Korea

Plant Disease ◽  
2021 ◽  
Author(s):  
Oliul Hassan ◽  
Taehyun Chang
Keyword(s):  
South Korea ◽  
Fusarium Oxysporum ◽  
Species Complex ◽  
Disease Incidence ◽  
Fusarium Species ◽  
Morphological Characteristics ◽  
Conidial Suspension ◽  
Sterile Water ◽  
Damping Off ◽  
First Report

In South Korea, ovate-leaf atractylodes (OLA) (Atractylodes ovata) is cultivated for herbal medicine. During May to June 2019, a disease with damping off symptoms on OLA seedlings were observed at three farmer fields in Mungyeong, South Korea. Disease incidence was estimated as approximately 20% based on calculating the proportion of symptomatic seedlings in three randomly selected fields. Six randomly selected seedlings (two from each field) showing damping off symptoms were collected. Small pieces (1 cm2) were cut from infected roots, surface-sterilized (1 minute in 0.5% sodium hypochlorite), rinsed twice with sterile water, air-dried and then plated on potato dextrose agar (PDA, Difco, and Becton Dickinson). Hyphal tips were excised and transferred to fresh PDA. Six morphologically similar isolates were obtained from six samples. Seven-day-old colonies, incubated at 25 °C in the dark on PDA, were whitish with light purple mycelia on the upper side and white with light purple at the center on the reverse side. Macroconidia were 3–5 septate, curved, both ends were pointed, and were 19.8–36.62 × 3.3–4.7 µm (n= 30). Microconidia were cylindrical or ellipsoid and 5.5–11.6 × 2.5–3.8 µm (n=30). Chlamydospores were globose and 9.6 –16.3 × 9.4 – 15.0 µm (n=30). The morphological characteristics of present isolates were comparable with that of Fusarium species (Maryani et al. 2019). Genomic DNA was extracted from 4 days old cultures of each isolate of SRRM 4.2, SRRH3, and SRRH5, EF-1α and rpb2 region were amplified using EF792 + EF829, and RPB2-5f2 + RPB2-7cr primer sets, respectively (Carbone and Kohn, 1999; O'Donnell et al. 2010) and sequenced (GenBank accession number: LC569791- LC569793 and LC600806- LC600808). BLAST query against Fusarium loci sampled and multilocus sequence typing database revealed that 99–100% identity to corresponding sequences of the F. oxysporum species complex (strain NRRL 28395 and 26379). Maximum likelihood phylogenetic analysis with MEGA v. 6.0 using the concatenated sequencing data for EF-1α and rpb2 showed that the isolates belonged to F. oxysporum species complex. Each three healthy seedlings with similar sized (big flower sabju) were grown for 20 days in a plastic pot containing autoclaved peat soil was used for pathogenicity tests. Conidial suspensions (106 conidia mL−1) of 20 days old colonies per isolate (two isolates) were prepared in sterile water. Three pots per strain were inoculated either by pouring 50 ml of the conidial suspension or by the same quantity of sterile distilled water as control. After inoculation, all pots were incubated at 25 °C with a 16-hour light/8-hour dark cycle in a growth chamber. This experiment repeated twice. Inoculated seedlings were watered twice a week. Approximately 60% of the inoculated seedlings per strain wilted after 15 days of inoculation and control seedlings remained asymptomatic. Fusarium oxysporum was successfully isolated from infected seedling and identified based on morphology and EF-1α sequences data to confirm Koch’s postulates. Fusarium oxysporum is responsible for damping-off of many plant species, including larch, tomato, melon, bean, banana, cotton, chickpea, and Arabidopsis thaliana (Fourie et al. 2011; Hassan et al.2019). To the best of our knowledge, this is the first report on damping-off of ovate-leaf atractylodes caused by F. oxysporum in South Korea. This finding provides a basis for studying the epidemic and management of the disease.

scholarly journals Determination of the relationship between epiphytes and selected filamentous bacteria in activated sludge

2016 ◽  
Author(s):  
◽  
Thobela Conco
Keyword(s):  
Electron Microscopy ◽  
Activated Sludge ◽  
Morphological Characteristics ◽  
Cell Interaction ◽  
Vital Role ◽  
Microbial Consortia ◽  
Filamentous Bacteria ◽  
Intrinsic Nature ◽  
Structural Support ◽  
Filamentous Cell

Activated sludge (AS) flocs are paramount in biological treatment of wastewater, are comprised of microbial consortia with organic and inorganic material bound together by extra polymeric substances (EPS). The filamentous bacteria play a vital role in the floc formation process by providing the necessary structural support. Presence of epiphytic attachment on selected filamentous bacteria is a commonly occurring phenomenon in activated sludge samples. Different theories have been proposed to describe this phenomenon; however, not much research has been carried out to explore the profundity of the attachment. In this study, an attempt has been made to elucidate the intrinsic nature of the epiphytic attachment between the bacterial rods and filamentous bacteria based on microscopic (morphological and structural) analysis. Characterization of these epiphytes were performed using fluorescence in situ hybridization (FISH) at group level using Alpha, Beta and Gamma Proteo-bacterial probes. Morphological characteristics of filament hosts and the bacterial rods at the interface region was assessed using scanning electron microscopy (SEM). The SEM micrographs indicated that the attachment was facilitated by more than the EPS layer. Further ultrastructural examination using transmission electron microscopy (TEM) indicated a possible cell-to-cell interaction between epiphytes and the selected filaments. Fibrillar structures resembling amyloid-like proteins were observed within the filament cell targeted by the epiphytes. An interaction was apparent between the amyloid like proteins and the epiphytes as exhibited by the direction of fibrillar structures pointing towards the approaching epiphytes. Common bacterial appendages such as pili and fimbria were absent at the interface and further noted was the presence of cell membrane extensions on the epiphytic bacteria protruding towards the targeted filamentous cell. The sheath of host filaments however, remained intact and unpenetrated, during colonization. Amyloid-like fibrils at interface may potentially play the role of attachment sites for the attaching epiphytes, as attachment facilitating appendages were not visualized.

scholarly journals Brazilian Amazonian palm-stem types and uses: a review

2021 ◽  
Vol 51 (4) ◽  
pp. 334-346
Author(s):  
Tatiani Yuriko Pinheiro KIKUCHI ◽  
Cátia Henriques CALLADO
Keyword(s):  
Native Species ◽  
Morphological Characteristics ◽  
Raw Material ◽  
Euterpe Oleracea ◽  
Stem Size ◽  
Income Sources ◽  
Palm Species ◽  
Renewable Raw Material ◽  
Sustainable Income ◽  
Socratea Exorrhiza

ABSTRACT Palms may be an important source of renewable raw material to replace wood, however, the uses of the stems of native species of the Brazil are known only at the local or regional level. We carried out a literature review on the traditional knowledge of the uses of the stems of palm species native to the Amazon biome in Brazil, and related the types of uses with morphological characteristics of the stems. The review resulted in information on 45 species with solitary or cespitose stems, and six stem-size types: tall (15 species), medium-short (3), medium (5), small (17), acaulescent (1) and climbing (4). We found 80 indications of stem use in seven categories and 14 subcategories. A similarity analysis showed that, in general, tall, medium-short, medium, small (≥ 10 cm in diameter) and climbing stem types, solitary or cespitous, are used for construction, furniture, handicrafts, utensils, tools and musical instruments. Only small stems (< 10 cm diameter) are used to manufacture weapons for hunting and fishing, and climbing stems are used in the manufacture of ropes. Stems of Socratea exorrhiza, Euterpe oleracea and Desmoncus polyacanthos are the most frequently used to meet subsistence needs in traditional communities in the Brazilian Amazon. Our findings indicate that there is a potential for use of several native palm stems as sources for alternative materials in the manufacture industry and as sustainable income sources for Amazonian communities.

scholarly journals Morphology, histochemical test, potential, and conservation effort of Alpinia warburgii K. Schum., a native species to Sulawesi

2021 ◽  
Vol 948 (1) ◽  
pp. 012014
Author(s):  
Trimanto ◽  
L Hapsari
Keyword(s):  
Native Species ◽  
Cell Structure ◽  
Morphological Characteristics ◽  
Anatomical Study ◽  
Anatomical Structure ◽  
Ex Situ ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Conservation Effort ◽  
Histochemical Tests

Abstract Alpinia warburgii is a species of the Zingiberaceae family native to Sulawesi. This study aims to characterize the plant morphology; analyze the anatomical structure, histochemical localization, and secretory cell structure of the rhizome; also discuss the potential and conservation effort of the species. Anatomical study and histochemical tests were observed microscopically using the fresh preparation method and wet mounted slide. Histochemical tests carried out include starch, protein, lipid, tannin, alkaloid, and flavonoid. This study is considered the first report of histochemical tests on A. warburgii. The main morphological characteristics of A. warburgii are perennial herbaceous; inflorescence terminal and raceme; flowers white, labellum obovate with red lines in the middle, and flanked by light green edges; fruit globose, green when young and yellow when ripe. The rhizome anatomical structure comprises the epidermis, endodermis, hypodermis, parenchyma, cortex, and vascular bundles, classified as closed collateral type. Histochemical tests showed positive localization for starch, protein, alkaloids, flavonoids, and tannins. The density of flavonoids secretory cells is higher than tannins and alkaloids, which indicates its potential as medicine to be associated with antioxidant, antimicrobial, antiinflammation, and anticancer. In addition to in-situ, ex-situ conservation effort through propagation is also important to maintain the species.

scholarly journals Morphological and Molecular Characteristics of Fungal Species Associated with Crown Rot of Strawberry in South Korea

2021 ◽  
Author(s):  
Oliul Hassan ◽  
Taehyun Chang
Keyword(s):  
South Korea ◽  
Root Rot ◽  
Species Complex ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Fungal Species ◽  
Crown Rot ◽  
Molecular Characteristics ◽  
Pathogenicity Test ◽  
Crown And Root Rot

Abstract Crown and root rot is the most important and destructive strawberry diseases in Korea as it causessubstantial economic loss. In August 2020, a severe outbreak of crown and root rot on strawberries (Fragaria×ananassa Duch.) was observed in the greenhouse at Sangju, South Korea. Infected plantlets displayed browning rot within the crown and root, stunted growth, and poor rooting. Thirty fungal isolates were procured from the affected plantlet. Isolates were identified based on morphological characteristics and pathogenicity test as well as sequence data obtained from internal transcribed spacer, large subunit ribosomal ribonucleic acid, translation elongation factor,and RNA polymerase Ⅱ-second largest subunit. Results showed that thecrown and root rot of strawberry in Korea was caused by three distinct fungal species:Fusarium oxysporum species complex, F. solani species complex, andPlectosphaerella cucumerina. To the best of our knowledge,F. solani species complex andP. cucumerinaare reported for the first time as the causal agents of the crown and root rot of strawberryin South Korea.Pathogenicity tests confirmed that these isolates are pathogenic to strawberry.Understanding the composition and biology of the pathogen population will be helpful toprovide effectivecontrol strategies for the disease.

scholarly journals Morphology and genome size of Epipactis helleborine (L.) Crantz (Orchidaceae) growing in anthropogenic and natural habitats

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5992 ◽  
Author(s):  
Agnieszka Rewicz ◽  
Monika Rewers ◽  
Iwona Jędrzejczyk ◽  
Tomasz Rewicz ◽  
Jeremi Kołodziejek ◽  
...  
Keyword(s):  
Genome Size ◽  
Native Species ◽  
Environmental Changes ◽  
Phenotypic Variability ◽  
Plant Cover ◽  
Morphological Characteristics ◽  
Nuclear Dna Content ◽  
Natural Habitats ◽  
Anthropogenic Habitats ◽  
Biometric Features

Background The process of apophytism or spreading native species to human-made habitats is one of the main elements in the creation of plant cover in anthropogenic areas. Lately, an increase of anthropogenic localities with valuable flora has been observed. Apophytes are also members of the family Orchidaceae, especially from the genus Epipactis. The aim of the study was to (i) determine and compare the phenotypic variation of E. helleborine (L.) Crantz plants in anthropogenic and natural habitats, (ii) compare the genome size of plants growing in natural and anthropogenic habitats. The results reported in this study may indicate that a habitat influences morphological characteristics of plant species. Methods Field studies were conducted on four native stands and four stands in anthropogenic areas of E. helleborine in Poland in years 2011–2013. Biometrical analyses were performed on shoots and flowers. The flowers were characterised by 25 biometric features and measured using a Nikon SMZ 800 binocular, microscopic Moticam-1SP cameras and the MIPlus07 programme (Conbest Co.). The nuclear DNA content was determined in fresh and young leaves of E. helleborine, collected from four natural and four anthropogenic populations. Results We observed that in anthropogenic populations: (i) shoots were higher than shoots from natural populations, (ii) flowers differed significantly in terms of ten biometric features between habitats, (iii) the genome size of some population differed significantly between plants growing in natural and anthropogenic habitats. Discussion According to some researchers, the presence of phenotypic variability and the occurrence of ecotypes are adaptation strategies of plants to environmental changes. In our opinion, in the case of the studied anthropogenic habitats (roadside) in which the E. helleborine populations grew, we can talk about ecofen due to the often repeated set of characteristic features, i.e., high shoots, long inflorescence and long, broad leaves. We agree, however, that it is difficult to isolate a taxonomic unit for ecofen due to the lack of experimental research.

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