scholarly journals ALKBH10B, an mRNA m6A Demethylase, Modulates ABA Response During Seed Germination in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun Tang ◽  
Junbo Yang ◽  
Hongchao Duan ◽  
Guifang Jia
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Seed Germination ◽  
Chemical Modification ◽  
Aba Signaling ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Aba Response

As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark N6-methyladenine (m6A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m6A demethylase and regulates floral transition. However, it is unclear whether ALKBH10B plays a role in abiotic stress response. Here, we found that the expression of ALKBH10B is increased in response to abscisic acid (ABA), osmotic, and salt stress. The alkbh10b mutants showed hypersensitive to ABA, osmotic, and salt stress during seed germination. Transcriptome analysis revealed that the expression of several ABA response genes is upregulated in alkbh10b-1 than that of wild type, indicating ALKBH10B negatively affects the ABA signaling. Furthermore, m6A sequencing showed that ABA signaling genes, including PYR1, PYL7, PYL9, ABI1, and SnRK2.2 are m6A hypermethylated in alkbh10b-1 after ABA treatment. Taken together, our work demonstrated that ALKBH10B negatively modulates ABA response during seed germination in Arabidopsis.

scholarly journals Antagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in Arabidopsis

2008 ◽  
Vol 20 (6) ◽  
pp. 1678-1692 ◽  
Author(s):  
Michiko Yasuda ◽  
Atsushi Ishikawa ◽  
Yusuke Jikumaru ◽  
Motoaki Seki ◽  
Taishi Umezawa ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Abiotic Stress ◽  
Stress Response ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Abiotic Stress Response ◽  
Antagonistic Interaction

scholarly journals An abscisic acid-responsive protein interaction network for sucrose non-fermenting related kinase1 in abiotic stress response

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Carina Steliana Carianopol ◽  
Aaron Lorheed Chan ◽  
Shaowei Dong ◽  
Nicholas J. Provart ◽  
Shelley Lumba ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Abiotic Stress ◽  
Stress Response ◽  
Stress Responses ◽  
Energy Homeostasis ◽  
Plant Stress ◽  
Interaction Network ◽  
Abiotic Stress Response ◽  
Functional Studies ◽  
Transcriptional Changes

AbstractYeast Snf1 (Sucrose non-fermenting1), mammalian AMPK (5′ AMP-activated protein kinase) and plant SnRK1 (Snf1-Related Kinase1) are conserved heterotrimeric kinase complexes that re-establish energy homeostasis following stress. The hormone abscisic acid (ABA) plays a crucial role in plant stress response. Activation of SnRK1 or ABA signaling results in overlapping transcriptional changes, suggesting these stress pathways share common targets. To investigate how SnRK1 and ABA interact during stress response in Arabidopsis thaliana, we screened the SnRK1 complex by yeast two-hybrid against a library of proteins encoded by 258 ABA-regulated genes. Here, we identify 125 SnRK1- interacting proteins (SnIPs). Network analysis indicates that a subset of SnIPs form signaling modules in response to abiotic stress. Functional studies show the involvement of SnRK1 and select SnIPs in abiotic stress responses. This targeted study uncovers the largest set of SnRK1 interactors, which can be used to further characterize SnRK1 role in plant survival under stress.

scholarly journals AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85208 ◽  
Author(s):  
An Yan ◽  
Minjie Wu ◽  
Limei Yan ◽  
Rui Hu ◽  
Imran Ali ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Seed Germination ◽  
Abiotic Stress Response

Growth, physiology, and abiotic stress response to abscisic acid in tomato seedlings

2015 ◽  
Vol 56 (3) ◽  
pp. 294-304 ◽  
Author(s):  
Ngoc-Thang Vu ◽  
Ho-Min Kang ◽  
Young-Shik Kim ◽  
Ki-Young Choi ◽  
Il-Seop Kim
Keyword(s):  
Abscisic Acid ◽  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response ◽  
Growth Physiology ◽  
Tomato Seedlings

scholarly journals OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice

2021 ◽  
Vol 22 (5) ◽  
pp. 2487
Author(s):  
Juyoung Choi ◽  
Wonkyung Lee ◽  
Gynheung An ◽  
Seong-Ryong Kim
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
In Silico Analysis ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Environmental Stress Response

Ubiquitination is an important environmental stress response, and E3 ubiquitin ligases play a major role in the process. T-DNA insertion mutants of rice, Oscbe1-1, and Oscbe1-2, were identified through the screening of cold stress tolerance at seedling stage. Oscbe1 mutants showed a significantly higher cold stress tolerance in the fresh weight, chlorophyll content, and photosynthetic efficiency than wild type. Molecular prediction showed that OsCBE1 (Oryza sativa Cullin4-Based E3 ubiquitin ligase1) encoded a novel substrate receptor of Cullin4-based E3 ubiquitin ligase complex (C4E3). Whereas Oscbe1 mutants had fewer panicles and grains than wild type in the paddy field, the overexpression lines of OsCBE1 had more panicles and grains, suggesting that OsCBE1 is involved in the regulation of both abiotic stress response and development. Oscbe1 mutants also showed ABA hypersensitivity during seed germination, suggesting OsCBE1 function for the stress response via ABA signaling. In silico analysis of OsCBE1 activity predicted a CCCH-type transcription factor, OsC3H32, as a putative substrate. Co-IP (Co-immunoprecipitation) study showed that OsCBE1 interacts with OsDDB1, an expected binding component of OsCBE1 and OsC3H32. Additionally, expression of OsOLE16, OsOLE18, and OsBURP5 were negatively related with expression of OsCBE1. These results suggest that OsCBE1 functions as a regulator of the abiotic stress response via CCCH as a member of the C4E3.

scholarly journals Mediator Complex: A Pivotal Regulator of ABA Signaling Pathway and Abiotic Stress Response in Plants

2020 ◽  
Vol 21 (20) ◽  
pp. 7755
Author(s):  
Leelyn Chong ◽  
Pengcheng Guo ◽  
Yingfang Zhu
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Rna Polymerase Ii ◽  
Signaling Pathway ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Current Knowledge ◽  
Mediator Complex ◽  
Aba Signaling ◽  
Abiotic Stress Response

As an evolutionarily conserved multi-protein complex, the Mediator complex modulates the association between transcription factors and RNA polymerase II to precisely regulate gene transcription. Although numerous studies have shown the diverse functions of Mediator complex in plant development, flowering, hormone signaling, and biotic stress response, its roles in the Abscisic acid (ABA) signaling pathway and abiotic stress response remain largely unclear. It has been recognized that the phytohormone, ABA, plays a predominant role in regulating plant adaption to various abiotic stresses as ABA can trigger extensive changes in the transcriptome to help the plants respond to environmental stimuli. Over the past decade, the Mediator complex has been revealed to play key roles in not only regulating the ABA signaling transduction but also in the abiotic stress responses. In this review, we will summarize current knowledge of the Mediator complex in regulating the plants’ response to ABA as well as to the abiotic stresses of cold, drought and high salinity. We will particularly emphasize the involvement of multi-functional subunits of MED25, MED18, MED16, and CDK8 in response to ABA and environmental perturbation. Additionally, we will discuss potential research directions available for further deciphering the role of Mediator complex in regulating ABA and other abiotic stress responses.

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