scholarly journals The Gene FvTST1 From Strawberry Modulates Endogenous Sugars Enhancing Plant Growth and Fruit Ripening

2022 ◽  
Vol 12 ◽  
Author(s):  
Arif Rashid ◽  
Haixiang Ruan ◽  
Yunsheng Wang
Keyword(s):  
Plant Growth ◽  
Fruit Ripening ◽  
Target Genes ◽  
Developmental Stages ◽  
Sugar Transport ◽  
Biological Significance ◽  
Sugar Accumulation ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway ◽  
Differential Expression Pattern

Sugar is an important carbon source and contributes significantly to the improvement of plant growth and fruit flavor quality. Sugar transport through the tonoplast is important for intracellular homeostasis and metabolic balance in plant cells. There are four tonoplast sugar transporters (FvTST1-4) in strawberry genome. The qRT-PCR results indicated that FvTST1 has a differential expression pattern in different tissues and developmental stages, and exhibited highest expression level in mature fruits. The yeast complementation assay showed that FvTST1 can mediate the uptake of different sugars, such as fructose, glucose, sucrose, and mannose. Subcellular localization analyses revealed that FvTST1 was mainly targeted to the tonoplast. Transient expression of FvTST1 in strawberry fruits enhanced both fruit ripening and sugar accumulation. Furthermore, FvTST1-transformed tomato plants exhibited higher sucrose and auxin content, enhanced seed germination and vegetative growth, higher photosynthetic rate, early flowering, and bore fruit; fructose and glucose levels were higher in transgenic fruits than those in the control. Transcriptomic analysis indicated that the auxin signaling pathway was highly enriched pathway in up-regulated Gene-ontology terms. In transgenic plants, genes encoding transcription factors, such as phytochrome-interacting factors PIF1, -3, and -4, as well as their potential target genes, were also induced. Collectively, the results show that FvTST1 enhances plant growth and fruit ripening by modulating endogenous sugars, and highlight the biological significance of this gene for future breeding purposes.

scholarly journals The NAC transcription factor FaRIF controls fruit ripening in strawberry

2021 ◽  
Author(s):  
Carmen Martín-Pizarro ◽  
José G Vallarino ◽  
Sonia Osorio ◽  
Victoriano Meco ◽  
María Urrutia ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Developmental Stages ◽  
Phenylpropanoid Pathway ◽  
Nac Transcription Factor ◽  
Sugar Accumulation ◽  
Nac Transcription Factors ◽  
Climacteric Fruit ◽  
Transgenic Lines ◽  
Functional Analyses

Abstract In contrast to climacteric fruits such as tomato, the knowledge on key regulatory genes controlling the ripening of strawberry, a non-climacteric fruit, is still limited. NAC transcription factors mediate different developmental processes in plants. Here, we identified and characterized FaRIF (Ripening Inducing Factor), a NAC transcription factor that is highly expressed and induced in strawberry receptacles during ripening. Functional analyses based on stable transgenic lines aimed at silencing FaRIF by RNA interference, either from a constitutive promoter or the ripe receptacle-specific EXP2 promoter, as well as overexpression lines showed that FaRIF controls critical ripening-related processes such as fruit softening and pigment and sugar accumulation. Physiological, metabolome and transcriptome analyses of receptacles of FaRIF-silenced and overexpression lines point to FaRIF as a key regulator of strawberry fruit ripening from early developmental stages, controlling abscisic acid (ABA) biosynthesis and signaling, cell wall degradation and modification, the phenylpropanoid pathway, volatiles production, and the balance of the aerobic/anaerobic metabolism. FaRIF is therefore a target to be modified/edited to control the quality of strawberry fruits.

scholarly journals Identification and Expression of SAUR Genes in the CAM Plant Agave

Genes ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 555
Author(s):  
Gang Deng ◽  
Xing Huang ◽  
Li Xie ◽  
Shibei Tan ◽  
Thomas Gbokie ◽  
...  
Keyword(s):  
Plant Growth ◽  
Stress Responses ◽  
Growth And Development ◽  
Developmental Stages ◽  
Auxin Signaling ◽  
Plant Growth And Development ◽  
Cam Plants ◽  
African Countries ◽  
Biotic Stresses ◽  
Species Specific

Agave species are important crassulacean acid metabolism (CAM) plants and widely cultivated in tropical areas for producing tequila spirit and fiber. The hybrid H11648 of Agave ((A. amaniensis × A. angustifolia) × A. amaniensis) is the main cultivar for fiber production in Brazil, China, and African countries. Small Auxin Up-regulated RNA (SAUR) genes have broad effect on auxin signaling-regulated plant growth and development, while only few SAUR genes have been reported in Agave species. In this study, we identified 43, 60, 24, and 21 SAUR genes with full-length coding regions in A. deserti, A. tequilana, A. H11648, and A. americana, respectively. Although phylogenetic analysis revealed that rice contained a species-specific expansion pattern of SAUR gene, no similar phenomena were observed in Agave species. The in silico expression indicated that SAUR genes had a distinct expression pattern in A. H11648 compared with other Agave species; and four SAUR genes were differentially expressed during CAM diel cycle in A. americana. Additionally, an expression analysis was conducted to estimate SAUR gene expression during different leaf developmental stages, abiotic and biotic stresses in A. H11648. Together, we first characterized the SAUR genes of Agave based on previously published transcriptome datasets and emphasized the potential functions of SAUR genes in Agave’s leaf development and stress responses. The identification of which further expands our understanding on auxin signaling-regulated plant growth and development in Agave species.

scholarly journals Small RNAs, Degradome, and Transcriptome Sequencing Provide Insights into Papaya Fruit Ripening Regulated by 1-MCP

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1643
Author(s):  
Jiahui Cai ◽  
Ziling Wu ◽  
Yanwei Hao ◽  
Yuanlong Liu ◽  
Zunyang Song ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Fruit Ripening ◽  
Transcriptome Sequencing ◽  
Target Genes ◽  
Enrichment Analysis ◽  
Functional Enrichment Analysis ◽  
Underlying Mechanism ◽  
Functional Enrichment ◽  
Auxin Signaling ◽  
Signal Pathways

As an inhibitor of ethylene receptors, 1-methylcyclopropene (1-MCP) can delay the ripening of papaya. However, improper 1-MCP treatment will cause a rubbery texture in papaya. Understanding of the underlying mechanism is still lacking. In the present work, a comparative sRNA analysis was conducted after different 1-MCP treatments and identified a total of 213 miRNAs, of which 44 were known miRNAs and 169 were novel miRNAs in papaya. Comprehensive functional enrichment analysis indicated that plant hormone signal pathways play an important role in fruit ripening. Through the comparative analysis of sRNAs and transcriptome sequencing, a total of 11 miRNAs and 12 target genes were associated with the ethylene and auxin signaling pathways. A total of 1741 target genes of miRNAs were identified by degradome sequencing, and nine miRNAs and eight miRNAs were differentially expressed under the ethylene and auxin signaling pathways, respectively. The network regulation diagram of miRNAs and target genes during fruit ripening was drawn. The expression of 11 miRNAs and 12 target genes was verified by RT-qPCR. The target gene verification showed that cpa-miR390a and cpa-miR396 target CpARF19-like and CpERF RAP2-12-like, respectively, affecting the ethylene and auxin signaling pathways and, therefore, papaya ripening.

scholarly journals Aux/IAA and ARF Gene Families in Salix suchowensis: Identification, Evolution, and Dynamic Transcriptome Profiling During the Plant Growth Process

2021 ◽  
Vol 12 ◽  
Author(s):  
Suyun Wei ◽  
Yingnan Chen ◽  
Jing Hou ◽  
Yonghua Yang ◽  
Tongming Yin
Keyword(s):  
Plant Growth ◽  
Growth Process ◽  
Transcriptome Profiling ◽  
Gene Families ◽  
Auxin Signaling ◽  
Vascular Differentiation ◽  
Auxin Signaling Pathway ◽  
Functional Involvement ◽  
Functional Features ◽  
Gene Structures

The phytohormone auxin plays a pivotal role in the regulation of plant growth and development, including vascular differentiation and tree growth. The auxin/indole-3-acetic acid (Aux/IAA) and auxin response transcription factor (ARF) genes are key components of plant auxin signaling. To gain more insight into the regulation and functional features of Aux/IAA and ARF genes during these processes, we identified 38 AUX/IAA and 34 ARF genes in the genome of Salix suchowensis and characterized their gene structures, conserved domains, and encoded amino acid compositions. Phylogenetic analysis of some typical land plants showed that the Aux/IAA and ARF genes of Salicaceae originated from a common ancestor and were significantly amplified by the ancestral eudicot hexaploidization event and the “salicoid” duplication that occurred before the divergence of poplar and willow. By analyzing dynamic transcriptome profiling data, some Aux/IAA and ARF genes were found to be involved in the regulation of plant growth, especially in the initial plant growth process. Additionally, we found that the expression of several miR160/miR167-ARFs was in agreement with canonical miRNA–ARF interactions, suggesting that miRNAs were possibly involved in the regulation of the auxin signaling pathway and the plant growth process. In summary, this study comprehensively analyzed the sequence features, origin, and expansion of Aux/IAA and ARF genes, and the results provide useful information for further studies on the functional involvement of auxin signaling genes in the plant growth process.

scholarly journals Characterization of RIN Isoforms and Their Expression in Tomato Fruit Ripening

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1739
Author(s):  
Maria A. Slugina ◽  
Gleb I. Efremov ◽  
Anna V. Shchennikova ◽  
Elena Z. Kochieva
Keyword(s):  
Fruit Ripening ◽  
Wild Species ◽  
Target Genes ◽  
Tomato Fruit ◽  
Expression Patterns ◽  
Fleshy Fruit ◽  
Sugar Accumulation ◽  
Gene Promoters ◽  
Tomato Fruit Ripening ◽  
Splicing Site

Ripening of tomato fleshy fruit is coordinated by transcription factor RIN, which triggers ethylene and carotenoid biosynthesis, sugar accumulation, and cell wall modifications. In this study, we identified and characterized complete sequences of the RIN chromosomal locus in two tomato Solanum lycopersicum cultivars, its rin/RIN genotype, and three wild green-fruited species differing in fruit color and composition. The results reveal that S. lycopersicum cultivars and some wild species (S. pennellii, S. habrochaites, and S. huaylasense) had a 3′-splicing site enabling the transcription of RIN1i and RIN2i isoforms. The other wild species (S. arcanum, S. chmielewskii, S. neorickii, and S. peruvianum) had a 3′-splicing site only for RIN2i, which was consistent with RIN1i and RIN2i expression patterns. The genotype rin/RIN, which had an extended 3′-terminal deletion in the rin allele, mainly expressed the chimeric RIN–MC transcript, which was also found in cultivars (RIN/RIN). The RIN1, but not RIN2, protein is able to induce the transcription of the reporter gene in the Y2H system, which positively correlated with the transcription profile of RIN1i and RIN target genes. We suggest that during fruit ripening, RIN1 activates ripening-related genes, whereas RIN2 and RIN–MC act as modulators by competing for RIN-binding sites in gene promoters, which should be confirmed by further studies on the association between RIN-splicing mechanisms and tomato fruit ripening.

scholarly journals Exogenous application of growth stimulators improves the condition of maize exposed to soil drought

2021 ◽  
Vol 43 (4) ◽  
Author(s):  
Agnieszka Ostrowska ◽  
Maciej T. Grzesiak ◽  
Tomasz Hura
Keyword(s):  
Plant Growth ◽  
Developmental Stages ◽  
Aminolevulinic Acid ◽  
Stem Elongation ◽  
Development Stage ◽  
Photochemical Activity ◽  
Soil Drought ◽  
Beneficial Effects ◽  
Induced Changes ◽  
The Impact

AbstractSoil drought is a major problem in plant cultivation. This is particularly true for thermophilic plants, such as maize, which grow in areas often affected by precipitation shortage. The problem may be alleviated using plant growth and development stimulators. Therefore, the aim of the study was to analyze the effects of 5-aminolevulinic acid (5-ALA), zearalenone (ZEN), triacontanol (TRIA) and silicon (Si) on water management and photosynthetic activity of maize under soil drought. The experiments covered three developmental stages: three leaves, stem elongation and heading. The impact of these substances applied during drought stress depended on the plant development stage. 5-ALA affected chlorophyll levels, gas exchange and photochemical activity of PSII. Similar effects were observed for ZEN, which additionally induced stem elongation and limited dehydration. Beneficial effects of TRIA were visible at the stage of three leaves and involved leaf hydration and plant growth. A silicon preparation applied at the same developmental stage triggered similar effects and additionally induced changes in chlorophyll levels. All the stimulators significantly affected transpiration intensity at the heading stage.

scholarly journals Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein–protein interaction network

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Suthanthiram Backiyarani ◽  
Rajendran Sasikala ◽  
Simeon Sharmiladevi ◽  
Subbaraya Uma
Keyword(s):  
Candidate Genes ◽  
Protein Interaction ◽  
Target Genes ◽  
Interaction Network ◽  
Enrichment Analysis ◽  
Auxin Signaling ◽  
Pathway Enrichment Analysis ◽  
Ppi Network ◽  
Protein Protein Interaction ◽  
The Difference

AbstractBanana, one of the most important staple fruit among global consumers is highly sterile owing to natural parthenocarpy. Identification of genetic factors responsible for parthenocarpy would facilitate the conventional breeders to improve the seeded accessions. We have constructed Protein–protein interaction (PPI) network through mining differentially expressed genes and the genes used for transgenic studies with respect to parthenocarpy. Based on the topological and pathway enrichment analysis of proteins in PPI network, 12 candidate genes were shortlisted. By further validating these candidate genes in seeded and seedless accession of Musa spp. we put forward MaAGL8, MaMADS16, MaGH3.8, MaMADS29, MaRGA1, MaEXPA1, MaGID1C, MaHK2 and MaBAM1 as possible target genes in the study of natural parthenocarpy. In contrary, expression profile of MaACLB-2 and MaZEP is anticipated to highlight the difference in artificially induced and natural parthenocarpy. By exploring the PPI of validated genes from the network, we postulated a putative pathway that bring insights into the significance of cytokinin mediated CLAVATA(CLV)–WUSHEL(WUS) signaling pathway in addition to gibberellin mediated auxin signaling in parthenocarpy. Our analysis is the first attempt to identify candidate genes and to hypothesize a putative mechanism that bridges the gaps in understanding natural parthenocarpy through PPI network.

scholarly journals QTL Across Fruit Development in Red Raspberry, A Study of A Primocane X Biennial Raspberry Population.

2021 ◽  
Author(s):  
Julie Graham ◽  
Kay Smith ◽  
Katrin MacKenzie ◽  
Linda Milne ◽  
Nikki Jennings ◽  
...  
Keyword(s):  
Linkage Group ◽  
Genetic Control ◽  
Fruit Ripening ◽  
Fruit Development ◽  
Developmental Stages ◽  
Developmental Process ◽  
Linkage Groups ◽  
Red Raspberry ◽  
Ripening Process ◽  
Second Year

Abstract Background The changing climate is altering timing of key fruit ripening processes and increasing the occurrence of fruit defects. This work aimed to expand our knowledge of the genetic control of the ripening process in raspberry by examining a biennial x primocane F1 population to determine if the progeny exhibited both primocane and biennial flowering modes, which if any was dominant, and to identify QTL and genome locations associated with fruit development to understand how developmental control in this population differs from a biennial x biennial F1 population previously studied. Results The progeny from this biennial x primocane population exhibited primocane fruiting completing their lifecycle in a single season and also fruiting on second-year wood not removed in season one. QTL associated with rate of fruit development were identified on both primocane and fruiting canes with both parents impacting. Conclusions Novel QTL associated with the developmental process of primocane fruiting were identified. These in the main, differed from developmental QTL for similar developmental stages on fruiting canes (second year canes) with only one significant overlap on linkage group 6. In general, the process of development on fruiting canes overall differed from that in a biennial x biennial population, with the differences being greatest on linkage groups 3 and 6 suggesting control of development differs in the different fruiting types. Further understanding will be achieved by examining genome regions linked to QTL to allow breeding to meet climate requirements for yield stability.

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