The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.)

Sulfur (S) fumigation is a commonly used sterilization method in horticultural facilities against fungal diseases. S fumigation damaged cucumber leaves, although the response mechanism is unclear. This study analyzes the growth, transcriptome, and metabolomic profiles of young and mature leaves, ovaries, and commercial cucumber fruits to decipher the mechanism of cucumber stress response under S fumigation. S fumigation significantly changed the photosynthetic efficiency and reactive oxygen species (ROS) in leaves, but not fruit development, fruit mass, and peel color. Transcriptome analysis indicated that S fumigation strongly regulated stress defense genes. The weighted gene co-expression network analysis revealed that S fumigation regulated ASPG1, AMC1 defense genes, LECRK3, and PERK1 protein kinase. The abscisic acid (ABA)-mediated model of regulation under S fumigation was constructed. Metabolome analysis showed that S fumigation significantly upregulated or downregulated the contents of amino acids, organic acids, sugars, glycosides, and lipids (VIP > 1 and P-value < 0.05). The opposite Pearson’s correlations of these differential metabolites implied that cucumber had different metabolic patterns in short-term and long-term S fumigation. Besides, the elevated levels of proline and triglyceride indicated that stress-responsive mechanisms existed in S-fumigated cucumber. Moreover, the comprehensive analysis indicated that S fumigation elevated secondary S-containing metabolites but decreased sulfate absorption and transportation in cucumber. Overall, our results provided a comprehensive assessment of S fumigation on cucumber, which laid the theoretical foundation for S fumigation in protected cultivation.

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Salinity Stress Responses and Adaptation Mechanisms in Eukaryotic Green Microalgae

Cells ◽

10.3390/cells8121657 ◽

2019 ◽

Vol 8 (12) ◽

pp. 1657 ◽

Cited By ~ 7

Author(s):

Prateek Shetty ◽

Margaret Mukami Gitau ◽

Gergely Maróti

Keyword(s):

Stress Responses ◽

High Salinity ◽

Freshwater Algae ◽

Short Term ◽

Adaptation Mechanisms ◽

Fundamental Research ◽

Ionic Imbalance ◽

Compare And Contrast ◽

Harsh Conditions

High salinity is a challenging environmental stress for organisms to overcome. Unicellular photosynthetic microalgae are especially vulnerable as they have to grapple not only with ionic imbalance and osmotic stress but also with the generated reactive oxygen species (ROS) interfering with photosynthesis. This review attempts to compare and contrast mechanisms that algae, particularly the eukaryotic Chlamydomonas microalgae, exhibit in order to immediately respond to harsh conditions caused by high salinity. The review also collates adaptation mechanisms of freshwater algae strains under persistent high salt conditions. Understanding both short-term and long-term algal responses to high salinity is integral to further fundamental research in algal biology and biotechnology.

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Stress responses of tomato fruit tissue submitted to massive doses of ionising radiation

Proceedings of the Royal Society of Edinburgh Section B Biological Sciences ◽

10.1017/s0269727000014019 ◽

1994 ◽

Vol 102 ◽

pp. 97-105 ◽

Cited By ~ 3

Author(s):

C. Triantaphylidès ◽

N. Banzet ◽

J. M. Ferullo ◽

C. Larrigaudière ◽

L. Nespoulous

Keyword(s):

Stress Responses ◽

Protein Metabolism ◽

Tomato Fruit ◽

Genetic Regulation ◽

Acc Synthase ◽

Ionising Radiation ◽

Short Term ◽

Induction Kinetics ◽

Global Protein Synthesis

AbstractIn plant tissue, massive doses of ionising radiation (0.5–3 kGy) induce an oxidative burst due to the overproduction of oxygen-centred free radicals. Changes in the protein metabolism of cherry tomato fruits were investigated in response to this peculiar stress. Although DNA damage definitively arrested cell division, the changes observed on a short-term basis were attributed to genetic regulation. Changes in protein metabolism were also maintained long term. Gamma-induced proteins (GIPs) were classified according to their induction kinetics. Group 1 proteins were induced immediately after the treatment and their synthesis was stopped within 24 h. During the same time period, global protein synthesis was restored and a new set of GIPs was induced. The function of these proteins is not yet known; but they may be involved in physiological disorders triggered by irradiation or in repair processes. Short-term typical changes involve the synthesis of ACC synthase – the ethylene pathway regulating enzyme - and most probably of some LMW-HSPs. A non-relevant response to irradiation has also been discovered, namely the long-term accumulation of chitinases. Irradiation induces both specific and non-specific responses which can be analysed by comparison with other types of oxidative stress and some GIPs seem to be specific to the treatment. The ability of irradiation to induce such different responses might be profitably applied for a better understanding of the oxidative mechanisms involved in signal transduction during environmental stress situations.

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Neuroendocrine modulation of stress response in the anuran, Rana esculenta

Amphibia-Reptilia ◽

10.1163/156853806778190141 ◽

2006 ◽

Vol 27 (3) ◽

pp. 401-408 ◽

Cited By ~ 15

Author(s):

Gilberto Mosconi ◽

Kazutoshi Yamamoto ◽

Sakae Kikuyama ◽

Marcello Carotti ◽

Francesco Palermo ◽

...

Keyword(s):

Salinity Stress ◽

Stress Responses ◽

Energy Demand ◽

Rana Esculenta ◽

Plasma Corticosterone ◽

Feedback Mechanism ◽

Negative Effects ◽

Short Term ◽

Wide Range

AbstractIn amphibians, as in other vertebrates, stress stimuli have been found to affect different functions, including development, growth, and reproduction. A wide range of responsiveness to stressors has been reported for amphibians; for instance, capture and/or captivity stress induced changes both in the hypothalamus-pituitary-interrenal and hypothalamus-pituitary-gonadal axes. However, few studies have examined the response to stress in terms of recovery and/or adaptation by applying stress paradigms for a short and long-term duration. In the present paper, the short-term captivity stress responses were evaluated in the anuran Rana esculenta by measuring peripheral corticosterone, androgens, prolactin (PRL), and growth hormone (GH) changes. Moreover, in long-term captivity and salinity stress, effects were evaluated by measuring peripheral PRL changes and those of PRL mRNA in the pituitary together with plasma corticosterone and androgens. Short-term (24 h) captivity stress induced an increase of peripheral corticosterone together with that of GH and PRL since these hormones are involved in the "alarm phase" and in energy demand of stressed animals. The opposite trend was found for peripheral androgens, in view of the negative effects exerted by stress in the reproductive axis. In long-term (1 month) captivity and salinity stress, responses were consistent with the increasing of PRL mRNA at pituitary level, through a long-loop feedback mechanism depending on the decreasing levels of peripheral PRL, whereas no changes were found in the levels of plasma corticosterone and androgens. Therefore, it seems that Rana esculenta activates different neuroendocrine mechanisms depending on the duration of stress and on the types of stressors.

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Supporting the supporter

Journal of Social and Personal Relationships ◽

10.1177/0265407516680500 ◽

2016 ◽

Vol 35 (2) ◽

pp. 202-223 ◽

Cited By ~ 5

Author(s):

Meara H. Faw

Keyword(s):

Social Support ◽

Stress Responses ◽

Physiological Stress ◽

Negative Effects ◽

Short Term ◽

Term Care ◽

The Social ◽

Significant Health

The social, economic, and physical costs associated with providing long-term care for a child with disabilities can be overwhelming, and it is not uncommon for caregivers to experience burnout, emotional distress, and significant health ailments as a result of their commitment to their child. Social support can be a key resource to combat these negative effects, as ample research has shown that social support can act as a buffer to the negative effects of stress. The current study explored whether short-term supportive interactions between parents of children with disabilities and members of their supportive network ( n = 40 dyads) influenced the physiological stress responses (as measured by salivary cortisol) of both interactants. Results indicated that receiving support in a short interaction resulted in parents experiencing decreases to their physiological stress, though the quality of the support played a key role in determining how beneficial the interaction was in this context. These results suggest the importance of considering support quality when examining the influence of social support on physical outcomes for support recipients.

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Conceptual short-term memory (CSTM) supports core claims of Christiansen and Chater

Behavioral and Brain Sciences ◽

10.1017/s0140525x15000928 ◽

2016 ◽

Vol 39 ◽

Author(s):

Mary C. Potter

Keyword(s):

Working Memory ◽

Rapid Serial Visual Presentation ◽

Language Comprehension ◽

Short Term Memory ◽

Visual Presentation ◽

Long Term Memory ◽

Short Term ◽

Term Memory ◽

Use Of Knowledge

AbstractRapid serial visual presentation (RSVP) of words or pictured scenes provides evidence for a large-capacity conceptual short-term memory (CSTM) that momentarily provides rich associated material from long-term memory, permitting rapid chunking (Potter 1993; 2009; 2012). In perception of scenes as well as language comprehension, we make use of knowledge that briefly exceeds the supposed limits of working memory.

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Ultrastructural investigations of MDL 19,660-induced effects on the canine platelet and megakaryocyte

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159412 ◽

1990 ◽

Vol 48 (3) ◽

pp. 374-375

Author(s):

D.E. Loudy ◽

J. Sprinkle-Cavallo ◽

J.T. Yarrington ◽

F.Y. Thompson ◽

J.P. Gibson

Keyword(s):

Antidepressant Drug ◽

Beagle Dogs ◽

Long Term Effects ◽

Short Term ◽

Platelet Counts ◽

Toxicological Studies ◽

Induced Aggregation ◽

Internal Architecture

Previous short term toxicological studies of one to two weeks duration have demonstrated that MDL 19,660 (5-(4-chlorophenyl)-2,4-dihydro-2,4-dimethyl-3Hl, 2,4-triazole-3-thione), an antidepressant drug, causes a dose-related thrombocytopenia in dogs. Platelet counts started to decline after two days of dosing with 30 mg/kg/day and continued to decrease to their lowest levels by 5-7 days. The loss in platelets was primarily of the small discoid subpopulation. In vitro studies have also indicated that MDL 19,660: does not spontaneously aggregate canine platelets and has moderate antiaggregating properties by inhibiting ADP-induced aggregation. The objectives of the present investigation of MDL 19,660 were to evaluate ultrastructurally long term effects on platelet internal architecture and changes in subpopulations of platelets and megakaryocytes.Nine male and nine female beagle dogs were divided equally into three groups and were administered orally 0, 15, or 30 mg/kg/day of MDL 19,660 for three months. Compared to a control platelet range of 353,000- 452,000/μl, a doserelated thrombocytopenia reached a maximum severity of an average of 135,000/μl for the 15 mg/kg/day dogs after two weeks and 81,000/μl for the 30 mg/kg/day dogs after one week.

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Comparison of Auditory, Language, Memory, and Attention Abilities in Children With and Without Listening Difficulties

American Journal of Audiology ◽

10.1044/2020_aja-20-00018 ◽

2020 ◽

Vol 29 (4) ◽

pp. 710-727

Author(s):

Beula M. Magimairaj ◽

Naveen K. Nagaraj ◽

Alexander V. Sergeev ◽

Natalie J. Benafield

Keyword(s):

Auditory Processing ◽

Short Term Memory ◽

Group Differences ◽

Long Term Memory ◽

Short Term ◽

Significant Group ◽

Term Memory ◽

Memory And Attention ◽

Speed And Accuracy

Objectives School-age children with and without parent-reported listening difficulties (LiD) were compared on auditory processing, language, memory, and attention abilities. The objective was to extend what is known so far in the literature about children with LiD by using multiple measures and selective novel measures across the above areas. Design Twenty-six children who were reported by their parents as having LiD and 26 age-matched typically developing children completed clinical tests of auditory processing and multiple measures of language, attention, and memory. All children had normal-range pure-tone hearing thresholds bilaterally. Group differences were examined. Results In addition to significantly poorer speech-perception-in-noise scores, children with LiD had reduced speed and accuracy of word retrieval from long-term memory, poorer short-term memory, sentence recall, and inferencing ability. Statistically significant group differences were of moderate effect size; however, standard test scores of children with LiD were not clinically poor. No statistically significant group differences were observed in attention, working memory capacity, vocabulary, and nonverbal IQ. Conclusions Mild signal-to-noise ratio loss, as reflected by the group mean of children with LiD, supported the children's functional listening problems. In addition, children's relative weakness in select areas of language performance, short-term memory, and long-term memory lexical retrieval speed and accuracy added to previous research on evidence-based areas that need to be evaluated in children with LiD who almost always have heterogenous profiles. Importantly, the functional difficulties faced by children with LiD in relation to their test results indicated, to some extent, that commonly used assessments may not be adequately capturing the children's listening challenges. Supplemental Material https://doi.org/10.23641/asha.12808607

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