scholarly journals Life Cycle and Genetic Identification of Argas persicus Infesting Domestic Fowl in Khyber Pakhtunkhwa, Pakistan

2021 ◽  
Vol 8 ◽  
Author(s):  
Hafsa Zahid ◽  
Sebastián Muñoz-Leal ◽  
Muhammad Qayash Khan ◽  
Abdulaziz S. Alouffi ◽  
Marcelo B. Labruna ◽  
...  
Keyword(s):  
Life Cycle ◽  
Domestic Fowl ◽  
The United States ◽  
Genetic Identification ◽  
16S Rrna Genes ◽  
Health Concern ◽  
Rrna Genes ◽  
Economic Losses ◽  
Local Alignment ◽  
Argas Persicus

Ticks transmit numerous pathogens to animals including humans; therefore, they are parasites of health concern. Soft ticks infesting domestic fowl in Pakistan are carriers of viruses and bacteria and cause unestimated economic losses in the poultry sector. The current study was intended to identify soft ticks infesting domestic fowl and understand their spatiotemporal distribution along 1 year. A sum of 7,219 soft ticks were collected from 608 domestic fowl in 58 infested shelters; 938 (12.9%) ticks were found on the host and 6,281 (87%) in the shelters. The collected ticks comprised 3,503 (48.52%) adults including 1,547 (21.42%) males and 1,956 (27.09%) females, 3,238 (44.85%) nymphs, and 478 (6.62%) larvae. The most prevalent life stages were adults, followed by nymphs and larvae. Overall tick prevalence considering all visited shelters was 38.66% (58/150). The highest tick prevalence was found in district Lakki Marwat (50.03%) followed by Peshawar (31.08%) and Chitral (18.88%) districts. All ticks were morpho-taxonomically identified as Argas persicus. To determine their life cycle, adult A. persicus were reared in the laboratory infesting domestic fowl (Gallus gallus domesticus). The life cycle was completed in 113–132 days (egg to egg) with a mean temperature of 33 ± 3°C and relative humidity of 65 ± 5%. Individual ticks were used for DNA extraction and subjected to polymerase chain reaction (PCR) using specific primers for the amplification of a partial fragment of mitochondrial cytochrome oxidase subunit I (cox1) and 16S ribosomal RNA (16S rRNA) genes. Obtained amplicons were compared using basic local alignment search tool (BLAST) to scan for homologous sequences. Phylogenetic trees showed A. persicus from Pakistan clustering with conspecific sequences reported from Australia, Chile, China, Kenya, and the United States. This is the first study aiming to reproduce the life cycle of A. persicus and genetically identify this tick in the region. Further studies are encouraged to investigate the pathogens associated with this soft tick species in Pakistan.

scholarly journals Descriptive Evidence of COVID-19’s Impact on Black LGBTQ Adults Amidst Ongoing Health Inequities

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. 1039-1039
Author(s):  
Charleigh Flohr ◽  
Cassandra Burton
Keyword(s):  
Human Rights ◽  
Employment Status ◽  
Online Survey ◽  
The United States ◽  
Health Concern ◽  
Economic Losses ◽  
Adult Participants ◽  
Important Health ◽  
Negative Impacts ◽  
Community Marketing

Abstract Black LGBTQ people significant challenges and discrimination as they face the barriers of living at the intersection of multiply marginalized identities, which have worsened during the coronavirus pandemic. At a baseline, LGBTQ people exhibit elevated risk of being negatively impacted by the pandemic across health, economic and other social outcomes (Cahill et al., 2020; Heslin & Hall, 2021; Human Rights Campaign, 2020). Black LGBTQ people have also suffered significant economic losses (Human Rights Campaign Foundation, 2020) . Methods The Human Rights Campaign Foundation and AARP supported and partnered with Community Marketing & Insights to conduct a survey of 1,815 Black LGBTQ adults in the United States. The online survey was fielded between September 21 and October 30, 2020. Many Black LGBTQ adult participants report healthcare discrimination in the last three years, with 19% reporting racial discrimination and 11% reporting sexual orientation-based discrimination. Furthermore, 31% of Black transgender adults report healthcare discrimination in the last three years. Preventing or treating COVID-19 is an important health concern to 67% of Black LGBTQ adults aged fifty-five and older, 63% of Black LGBTQ adults aged thirty-five to fifty-four and 53% aged eighteen to thirty-four. Overall, Black LGBTQ adults report being significantly impacted by the pandemic, including negative impacts on their social health (60%), mental health (44%), the physical health of their close friends and family (33%), their finances (30%), and their employment status (22%). Overall, one-quarter (25%) of Black LGBTQ adults had at least one close friend or family member die from coronavirus illness.

scholarly journals Mediation Analysis as a Means of Identifying Dietary Components That Differentially Affect the Fecal Microbiota of Infants Weaned by Modified Baby-Led and Traditional Approaches

2018 ◽  
Vol 84 (18) ◽  
Author(s):  
Claudia Leong ◽  
Jillian J. Haszard ◽  
Blair Lawley ◽  
Anna Otal ◽  
Rachael W. Taylor ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Fiber ◽  
Complementary Feeding ◽  
Alpha Diversity ◽  
The United States ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Content Type ◽  
Diet Records ◽  
Dietary Components

ABSTRACT The introduction of “solids” (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a “baby-led” approach to complementary feeding that encourages the early introduction of an adult-type diet results in alterations of the gut microbiota composition compared to traditional spoon-feeding. The Baby-Led Introduction to SolidS (BLISS) study randomized 206 infants to BLISS (a modified version of baby-led weaning [BLW], the introduction of solids at 6 months of age, followed by self-feeding of family foods) or control (traditional spoon-feeding of purées) groups. Fecal microbiotas and 3-day weighed-diet records were analyzed for a subset of 74 infants at 7 and 12 months of age. The composition of the microbiota was determined by sequencing of 16S rRNA genes amplified by PCR from bulk DNA extracted from feces. Diet records were used to estimate food and dietary fiber intake. Alpha diversity (number of operational taxonomic units [OTUs]) was significantly lower in BLISS infants at 12 months of age (difference [95% confidence interval {CI}] of 31 OTUs [3.4 to 58.5]; P = 0.028), and while there were no significant differences between control and BLISS infants in relative abundances of Bifidobacteriaceae, Enterobacteriaceae, Veillonellaceae, Bacteroidaceae, Erysipelotrichaceae, Lachnospiraceae, or Ruminococcaceae at 7 or 12 months of age, OTUs representing the genus Roseburia were less prevalent in BLISS microbiotas at 12 months. Mediation models demonstrated that the intake of “fruit and vegetables” and “dietary fiber” explained 29% and 25%, respectively, of the relationship between group (BLISS versus control) and alpha diversity. IMPORTANCE The introduction of solid foods (complementary feeding or weaning) to infants leads to more-complex compositions of microbial communities (microbiota or microbiome) in the gut. In baby-led weaning (BLW), infants are given only finger foods that they can pick up and feed themselves—there is no parental spoon-feeding of puréed baby foods—and infants are encouraged to eat family meals. BLW is a new approach to infant feeding that is increasing in popularity in the United States, New Zealand, the United Kingdom, and Canada. We used mediation modeling, commonly used in health research but not in microbiota studies until now, to identify particular dietary components that affected the development of the infant gut microbiota.

scholarly journals Genetic identification of hake and megrim eggs in formaldehyde-fixed plankton samples

2005 ◽  
Vol 62 (5) ◽  
pp. 908-914 ◽  
Author(s):  
Juliana Perez ◽  
Paula Álvarez ◽  
Jose L. Martinez ◽  
Eva Garcia-Vazquez
Keyword(s):  
Stock Assessment ◽  
Pcr Amplification ◽  
Species Specificity ◽  
Genetic Identification ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Merluccius Merluccius ◽  
Commercially Important ◽  
Eggs And Larvae ◽  
European Hake

Abstract Spawning of European hake (Merluccius merluccius) and megrim (Lepidorhombus whiffiagonis and L. boscii) overlap in time and space. Their eggs are morphologically similar, so genetic identification is needed for purposes of stock assessment based on plankton surveys. Amplification fragment sizes of a partial sequence within the 16S rRNA genes are different for Merluccius merluccius, Lepidorhombus boscii, and L. whiffiagonis. Species-specificity of the pattern was confirmed after analysing adult individuals from all distribution areas of the three species. After Chelex-based DNA extraction and PCR amplification, fragment sizes at this gene were successfully determined from 85% formaldehyde-fixed eggs and larvae recovered from plankton samples. Species-specificity was resolved using an ABI genetic analyser. The results were 100% reproducible. This methodology for genetic identification of hake and megrim eggs can be very useful for stock assessment of these three commercially important fish species.

scholarly journals Tick-Borne Hemoparasites of Sheep: A Molecular Research in Turkey

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 162
Author(s):  
Onur Ceylan ◽  
Benedicto Byamukama ◽  
Ceylan Ceylan ◽  
Eloiza May Galon ◽  
Mingming Liu ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Control Measures ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Economic Losses ◽  
Anaplasma Ovis ◽  
Subtropical Zone ◽  
Theileria Lestoquardi ◽  
Geographical Regions ◽  
Babesia Ovis

Tick-borne diseases (TBDs) indulge in severe economic losses in the livestock industry by adversely affecting the small ruminant breeding in tropical and subtropical zone countries, including Turkey. Turkey encompasses a wide land area representing diverse climatic conditions. The present study explored the presence and distribution of Babesia ovis, Theileria ovis, Theileria lestoquardi, Anaplasma ovis, Anaplasma phagocytophilum and the co-occurrence status of these pathogens. A total of 299 sheep blood samples were collected from fifteen provinces located in six different geographical regions in Turkey. PCR analyses were executed using species-specific primers based on Babesia ovis BoSSU rRNA, Theileria ovis ToSSU rRNA, Theileria lestoquardi 18S rRNA, Anaplasma ovis Major Surface Protein (AoMSP4), and Anaplasma phagocytophilum 16S rRNA genes. Overall, 219 (73.24%) sheep were found to be infected with at least one of the following protozoan and rickettsial pathogens; B. ovis, A. ovis,T. ovis, and A. phagocytophilum. Theileria lestoquardi was not detected in any blood sample. The global prevalence of B. ovis, A. ovis, T. ovis, and A. phagocytophilum was estimated to be 2.68%, 16.05%, 41.47%, and 57.19%, respectively. Besides this, dual (24.41%), triple (9.03%), and quadruple (0.67%) co-infections were detected in the study. Phylogenetic analysis revealed significant nucleotide sequence identities between the sequences obtained in this study and the sequences registered in the GenBank. This study provides relevant data regarding the predominance of ovine tick-borne protozoan and rickettsial agents in Turkey. A high molecular prevalence of tick-borne pathogens (TBPs) was identified in the study. This situation indicates that TBPs should be screened continuously, and necessary control measures should be taken to prevent diseases caused by tick-borne protozoan and rickettsial agents.

scholarly journals Diversity Converges During Community Assembly In Methanogenic Granules, Suggesting A Biofilm Life-Cycle

2018 ◽  
Author(s):  
Anna Christine Trego ◽  
Cristina Morabito ◽  
Isabelle Bourven ◽  
Gilles Guibaud ◽  
Simon Mills ◽  
...  
Keyword(s):  
Life Cycle ◽  
Community Assembly ◽  
Linear Convergence ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Core Microbiome ◽  
Methanogenic Activity ◽  
Size Fractions ◽  
Physico Chemical ◽  
Engineered Systems

Anaerobic biological decomposition of organic matter is ubiquitous in Nature wherever anaerobic environments prevail, and is catalysed by hydrolytic, fermentative, acetogenic, methanogenic, and various other groups, including syntrophic bacteria. It is also harnessed in innovative ways in engineered systems that may rely on small (0.1-4.0 mm), spherical, anaerobic granules, which we have found to be highly-replicated, whole-ecosystems harbouring the entire community necessary to mineralise complex organics. We hypothesised distinct granule sizes correspond to stages in a biofilm life-cycle, in which small granules are young and larger ones are old. Here, granules were separated into 10 size fractions used for physico-chemical and ecological characterisation. Gradients of volatile solids, density, settleability, biofilm morphology, methanogenic activity, and EPS profiles were observed across size fractions. Sequencing of 16S rRNA genes indicated linear convergence of diversity during community assembly as granules increased in size. A total of 155 discriminant OTUs were identified, and correlated strongly with physico-chemical parameters. Community assembly across sizes was influenced by a niche effect, whereby Euryarchaeota dominated a core microbiome presumably as granules became more anaerobic. The findings indicate opportunities for precision management of environmental biotechnologies, and the potential of aggregates as playgrounds to study assembly and succession in whole microbiomes.

scholarly journals Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics

2021 ◽  
Vol 8 ◽  
Author(s):  
Bin Xu ◽  
Xi Chen ◽  
Fengying Lu ◽  
Yu Sun ◽  
Huawei Sun ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Molecular Diagnostic ◽  
Economic Losses ◽  
Clinical Samples ◽  
Comparative Genomic ◽  
Evolutionary Analysis ◽  
Genome Sequences ◽  
Mycoplasma Synoviae

Mycoplasma synoviae is an important pathogen of poultry, causing significant economic losses in this industry. Analysis of the unique genes and shared genes among different M. synoviae strains and among related species is helpful for studying the molecular pathogenesis of M. synoviae and provides valuable molecular diagnostic targets to facilitate the identification of M. synoviae species. We selected a total of 46 strains, including six M. synoviae strains, from 25 major animal (including avian) Mycoplasma species/subspecies that had complete genome sequences and annotation information published in GenBank, and used them for comparative genomic analysis. After analysis, 16 common genes were found in the 46 strains. Thirteen single-copy core genes and the 16s rRNA genes were used for genetic evolutionary analysis. M. synoviae was found to have a distant evolutionary relationship not only with other arthritis-causing mycoplasmas, but also with another major avian pathogen, Mycoplasma gallisepticum, that shares the major virulence factor vlhA with M. synoviae. Subsequently, six unique coding genes were identified as shared among these M. synoviae strains that are absent in other species with published genome sequences. Two of the genes were found to be located in the genetically stable regions of the genomes of M. synoviae and were determined to be present in all M. synoviae isolated strains (n = 20) and M. synoviae-positive clinical samples (n = 48) preserved in our laboratory. These two genes were used as molecular diagnostic targets for which SYBR green quantitative PCR detection methods were designed. The two quantitative PCR methods exhibited good reproducibility and high specificity when tested on positive plasmid controls and genomic DNA extracted from different M. synoviae strains, other major avian pathogenic bacteria/mycoplasmas, and low pathogenic Mycoplasma species. The detection limit for the two genes was 10 copies or less per reaction. The clinical sensitivity and specificity of the quantitative PCR methods were both 100% based on testing chicken hock joint samples with positive or negative M. synoviae infection. This research provides a foundation for the study of species-specific differences and molecular diagnosis of M. synoviae.

scholarly journals Efficiency of boiling and four other methods for genomic DNA extraction of deteriorating spore-forming bacteria from milk

2016 ◽  
Vol 37 (5) ◽  
pp. 3069 ◽  
Author(s):  
Jose Carlos Ribeiro Junior ◽  
Ronaldo Tamanini ◽  
Bruna Fritegoto Soares ◽  
Aline Marangon de Oliveira ◽  
Fernando De Godoi Silva ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
The United States ◽  
Enzymatic Digestion ◽  
Molecular Techniques ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Genomic Dna Extraction ◽  
Commercial Kit

The spore-forming microbiota is mainly responsible for the deterioration of pasteurized milk with long shelf life in the United States. The identification of these microorganisms, using molecular tools, is of particular importance for the maintenance of the quality of milk. However, these molecular techniques are not only costly but also labor-intensive and time-consuming. The aim of this study was to compare the efficiency of boiling in conjunction with four other methods for the genomic DNA extraction of sporulated bacteria with proteolytic and lipolytic potential isolated from raw milk in the states of Paraná and Maranhão, Brazil. Protocols based on cellular lysis by enzymatic digestion, phenolic extraction, microwave-heating, as well as the use of guanidine isothiocyanate were used. This study proposes a method involving simple boiling for the extraction of genomic DNA from these microorganisms. Variations in the quality and yield of the extracted DNA among these methods were observed. However, both the cell lysis protocol by enzymatic digestion (commercial kit) and the simple boiling method proposed in this study yielded sufficient DNA for successfully carrying out the Polymerase Chain Reaction (PCR) of the rpoB and 16S rRNA genes for all 11 strains of microorganisms tested. Other protocols failed to yield sufficient quantity and quality of DNA from all microorganisms tested, since only a few strains have showed positive results by PCR, thereby hindering the search for new microorganisms. Thus, the simple boiling method for DNA extraction from sporulated bacteria in spoiled milk showed the same efficacy as that of the commercial kit. Moreover, the method is inexpensive, easy to perform, and much less time-consuming.

Oral Microbiome in Children with Caries: A Systematic Review

2020 ◽  
Vol 981 ◽  
pp. 271-279
Author(s):  
Muhammad Zaki Ramli ◽  
Nina Suhaity Azmi
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Public Health Problem ◽  
The United States ◽  
Oral Microbiome ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Careful Examination ◽  
Future Research ◽  
Childhood Caries

Oral caries remains a significant public health problem and affects children with early childhood caries (ECC) and severe early childhood caries (S-ECC). However, the mechanisms of these complex multi-species communities are not well understood. This article systematically reviews the studies involving children with caries to learn the demographics of the subjects, their subjects and types of sample, the methodological approaches undertaken, the main findings, and eventually to make recommendations for future research. A search using a designated search string in the Web of Science resulted in 148 articles. A careful examination of each article based on the inclusion criteria resulted in a final 40 articles. Most of the studies were conducted in the United States and China. The studies involved mostly, but not exclusively, children with primary dentition and were having caries. Different types of subjects were sampled from different racial backgrounds and diseases. Different samples were collected, such as supragingival plaque, saliva and biofilm. Clonal analyses and anaerobic approaches were used to characterise the oral microbiome, including shotgun sequencing, pyrosequencing of 16S rRNA genes, and Pacbio sequencing. Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria were among the phyla commonly reported by the studies. Streptococcus mutans was a key aetiologic agent in the development of caries, besides Veillonella, Prevotella spp. and Lactobacillus spp. Some studies developed models and discussed the applications of oral microbiome in relation to diseases. We recommend further reviews on studies strictly on children with caries and studies involving children only for a better generalisation of the findings. More studies relating oral microbiome with dietary intake and other diseases can also be conducted.

scholarly journals FILOGENI MOLEKULER BAKTERI DARI MEDIA PEMELIHARAAN ROTIFER YANG DIBERI OLAHAN LIMBAH IKAN SEBAGAI SUMBER NUTRISI

2021 ◽  
Vol 9 (1) ◽  
pp. 38
Author(s):  
Herlin S Hubu ◽  
Stenly Wullur ◽  
Veibe Warouw ◽  
Elvy L Ginting ◽  
Robert A Bara ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Species ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Local Alignment ◽  
Rrna Gene ◽  
Fish Waste ◽  
Positive Effects ◽  
The 16S Rrna Gene

This study aims to identify and construct molecular phylogeny of an isolate bacteria from culture media of rotifer Brachionus rotudiforis supplied with processed fishery waste feed as nutritional source. The use of fish waste-based food for rotifer showed positive effects on growth and nutrient content of the rotifers. Genomic DNA of the isolate bacteria BRLI- 01 was extracted and the 16S rRNA gene was amplified using primers (8F and 1492F) and further sequenced using Sanger sequence technique. The 16S rRNA gene was analysed using SeqScanner® and MEGA® followed with BLAST (Basic Local Alignment Search Tool) analyses in the NCBI (National Centre for Biotechnology Information). Amplification result of 16S rRNA gene bacteria s NCBI site as a reference for identification and phylogeny of bacterial species. BRLI-01 was successfully cultured on rotifer rearing media. The results of the 16S rRNA gene amplification of the isolate bacteria showed a DNA band with a length of 1400 bp. The BLAST result on the NCBI showed that the isolate bacteria BRLI-01 had a percent identity (98.46%) and is in the same phylogony branching position with Vibrio rotiferianus Keywords: Rotifers, Bacteria, Fish waste, 16S rRNA Genes, Phylogeny identification

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