scholarly journals Low Lignin Mutants and Reduction of Lignin Content in Grasses for Increased Utilisation of Lignocellulose

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 256 ◽  
Author(s):  
Cecilie S. L. Christensen ◽  
Søren K. Rasmussen
Keyword(s):  
Animal Feed ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Biofuel Production ◽  
Phenotypic Traits ◽  
Monolignol Biosynthesis ◽  
Genes Encoding ◽  
Regulated Gene Expression ◽  
Mutant Lines ◽  
The Impact

Biomass rich in lignocellulose from grasses is a major source for biofuel production and animal feed. However, the presence of lignin in cell walls limits its efficient utilisation such as in its bioconversion to biofuel. Reduction of the lignin content or alteration of its structure in crop plants have been pursued, either by regulating genes encoding enzymes in the lignin biosynthetic pathway using biotechnological techniques or by breeding naturally-occurring low lignin mutant lines. The aim of this review is to provide a summary of these studies, focusing on lignin (monolignol) biosynthesis and composition in grasses and, where possible, the impact on recalcitrance to bioconversion. An overview of transgenic crops of the grass family with regulated gene expression in lignin biosynthesis is presented, including the effect on lignin content and changes in the ratio of p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S) units. Furthermore, a survey is provided of low-lignin mutants in grasses, including cereals in particular, summarising their origin and phenotypic traits together with genetics and the molecular function of the various genes identified.

scholarly journals Patatin-Related Phospholipase AtpPLAIIIα Affects Lignification of Xylem in Arabidopsis and Hybrid Poplars

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 451 ◽  
Author(s):  
Jin Hoon Jang ◽  
Ok Ran Lee
Keyword(s):  
Cell Walls ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Biofuel Production ◽  
Amide Bonds ◽  
Hybrid Poplars ◽  
Plant Lipids ◽  
Lipid Acyl Hydrolase ◽  
Specific Regulation

Lipid acyl hydrolase are a diverse group of enzymes that hydrolyze the ester or amide bonds of fatty acid in plant lipids. Patatin-related phospholipase AIIIs (pPLAIIIs) are one of major lipid acyl hydrolases that are less closely related to potato tuber patatins and are plant-specific. Recently, overexpression of ginseng-derived PgpPLAIIIβ was reported to be involved in the reduced level of lignin content in Arabidopsis and the mature xylem layer of poplar. The presence of lignin-polysaccharides renders cell walls recalcitrant for pulping and biofuel production. The tissue-specific regulation of lignin biosynthesis, without altering all xylem in plants, can be utilized usefully by keeping mechanical strength and resistance to various environmental stimuli. To identify another pPLAIII homolog from Arabidopsis, constitutively overexpressed AtpPLAIIIα was characterized for xylem lignification in two well-studied model plants, Arabidopsis and poplar. The characterization of gene function in annual and perennial plants with respect to lignin biosynthesis revealed the functional redundancy of less lignification via downregulation of lignin biosynthesis-related genes.

scholarly journals Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10741
Author(s):  
Nan Chao ◽  
Qi Qi ◽  
Shuang Li ◽  
Brent Ruan ◽  
Xiangning Jiang ◽  
...  
Keyword(s):  
Shikimic Acid ◽  
Lignin Content ◽  
Expression Profiles ◽  
Populus Trichocarpa ◽  
Functional Divergence ◽  
Lignin Biosynthesis ◽  
Populus Tomentosa ◽  
Motif Analysis ◽  
Monolignol Biosynthesis ◽  
Populus Tomentosa Carr

Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates.

scholarly journals Genetic, transcriptional, and regulatory landscape of monolignol biosynthesis pathway in Miscanthus × giganteus

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xiaofei Zeng ◽  
Jiajing Sheng ◽  
Fenglin Zhu ◽  
Tianzi Wei ◽  
Lingling Zhao ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Enzymatic Reactions ◽  
Biosynthesis Pathway ◽  
Miscanthus Giganteus ◽  
Monolignol Biosynthesis ◽  
Essential Knowledge ◽  
Main Component ◽  
Regulatory Landscape

Abstract Background Miscanthus × giganteus is widely recognized as a promising lignocellulosic biomass crop due to its advantages of high biomass production, low environmental impacts, and the potential to be cultivated on marginal land. However, the high costs of bioethanol production still limit the current commercialization of lignocellulosic bioethanol. The lignin in the cell wall and its by-products released in the pretreatment step is the main component inhibiting the enzymatic reactions in the saccharification and fermentation processes. Hence, genetic modification of the genes involved in lignin biosynthesis could be a feasible strategy to overcome this barrier by manipulating the lignin content and composition of M. × giganteus. For this purpose, the essential knowledge of these genes and understanding the underlying regulatory mechanisms in M. × giganteus is required. Results In this study, MgPAL1, MgPAL5, Mg4CL1, Mg4CL3, MgHCT1, MgHCT2, MgC3′H1, MgCCoAOMT1, MgCCoAOMT3, MgCCR1, MgCCR2, MgF5H, MgCOMT, and MgCAD were identified as the major monolignol biosynthetic genes in M. × giganteus based on genetic and transcriptional evidence. Among them, 12 genes were cloned and sequenced. By combining transcription factor binding site prediction and expression correlation analysis, MYB46, MYB61, MYB63, WRKY24, WRKY35, WRKY12, ERF021, ERF058, and ERF017 were inferred to regulate the expression of these genes directly. On the basis of these results, an integrated model was summarized to depict the monolignol biosynthesis pathway and the underlying regulatory mechanism in M. × giganteus. Conclusions This study provides a list of potential gene targets for genetic improvement of lignocellulosic biomass quality of M. × giganteus, and reveals the genetic, transcriptional, and regulatory landscape of the monolignol biosynthesis pathway in M. × giganteus.

Forages for feedstocks of biorefineries in temperate environments: review of lignin research in bioenergy crops and some insight into Miscanthus studies

2014 ◽  
Vol 65 (11) ◽  
pp. 1199 ◽  
Author(s):  
Maria S. Dwiyanti ◽  
J. Ryan Stewart ◽  
Toshihiko Yamada
Keyword(s):  
Panicum Virgatum ◽  
Target Genes ◽  
Lignin Content ◽  
Genetic Regulation ◽  
Warm Season ◽  
Lignin Biosynthesis ◽  
Biofuel Production ◽  
Bioenergy Crops ◽  
Cost Efficient ◽  
Insight Into

Rhizomatous and perennial warm-season C4 grasses such as Miscanthus spp. and switchgrass (Panicum virgatum) are potential bioenergy crops for temperate regions. However, lignin in Miscanthus and switchgrass inhibits the cellulose digestion process during bioethanol production. One of the targets for improvement of forages from feedstocks to bioenergy crops is to develop a cost-efficient biorefinery process through lignin content manipulation. Numerous reports have shown that RNAi suppression of lignin-biosynthesis pathway genes can increase biomass fermentable sugar yields for biofuel production. These studies have also reported that RNAi suppression of cell-wall lignin biosynthesis can decrease biomass yield and resistance to biotic stress in the transgenic plants. Transcriptome and metabolome approaches can be used to clarify the networks and pathways of lignin biosynthesis to facilitate the identification of appropriate target genes for transformation. However, whole-genome sequencing of the forage species, which provides much-needed genomic information, is limited. Germplasm of natural, low-lignin mutants also plays a role in identification of genetic regulation of lignin content and this would be useful breeding material. Molecular markers have been developed and utilised to accelerate identification of quantitative trait loci/genes for traits relating to the biorefinery process. All of these studies will serve as basic information for supporting genetic improvement through classical breeding or genetic transformation, and offer the opportunity to develop cultivars which have enhanced biomass and are cost-efficient for biorefinery process.

scholarly journals CRISPR-Knockout of CSE Gene Improves Saccharification Efficiency by Reducing Lignin Content in Hybrid Poplar

2021 ◽  
Vol 22 (18) ◽  
pp. 9750
Author(s):  
Hyun-A Jang ◽  
Eun-Kyung Bae ◽  
Min-Ha Kim ◽  
Su-Jin Park ◽  
Na-Young Choi ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lignin Content ◽  
Hybrid Poplar ◽  
Lignin Biosynthesis ◽  
Biofuel Production ◽  
Guide Rnas ◽  
Hybrid Poplars ◽  
Four Seasons ◽  
Promising Target ◽  
Saccharification Efficiency

Caffeoyl shikimate esterase (CSE) has been shown to play an important role in lignin biosynthesis in plants and is, therefore, a promising target for generating improved lignocellulosic biomass crops for sustainable biofuel production. Populus spp. has two CSE genes (CSE1 and CSE2) and, thus, the hybrid poplar (Populus alba × P. glandulosa) investigated in this study has four CSE genes. Here, we present transgenic hybrid poplars with knockouts of each CSE gene achieved by CRISPR/Cas9. To knockout the CSE genes of the hybrid poplar, we designed three single guide RNAs (sg1–sg3), and produced three different transgenic poplars with either CSE1 (CSE1-sg2), CSE2 (CSE2-sg3), or both genes (CSE1/2-sg1) mutated. CSE1-sg2 and CSE2-sg3 poplars showed up to 29.1% reduction in lignin deposition with irregularly shaped xylem vessels. However, CSE1-sg2 and CSE2-sg3 poplars were morphologically indistinguishable from WT and showed no significant differences in growth in a long-term living modified organism (LMO) field-test covering four seasons. Gene expression analysis revealed that many lignin biosynthetic genes were downregulated in CSE1-sg2 and CSE2-sg3 poplars. Indeed, the CSE1-sg2 and CSE2-sg3 poplars had up to 25% higher saccharification efficiency than the WT control. Our results demonstrate that precise editing of CSE by CRISPR/Cas9 technology can improve lignocellulosic biomass without a growth penalty.

Groundnut Shells as a Potential Feed Supplement for Ruminants on Pastures: A Review

2021 ◽  
Author(s):  
B.G. Mokolopi
Keyword(s):  
Animal Feed ◽  
Lignin Content ◽  
Poor Quality ◽  
Biofuel Production ◽  
Feed Supplement ◽  
Short Supply ◽  
Industrial Processing ◽  
Arachis Hypogea ◽  
Groundnut Shell ◽  
High Lignin Content

Communal grazing does not offer adequate forage for ruminants throughout the year. This problem is exacerbated during the dry season when grazing is scarce and of poor nutritional quality. Mineral shortages are common in communal grazing environments and yet they are nutritional requirements for optimal development, physiologic functioning and productivity in animals, as well as for cattle growth, reproduction and health. However, the use of groundnut (Arachis hypogea L.) shell (GNS) that are readily available but have no direct nutritional benefit in humans, have not been extensively investigated as a potential source of animal feed. This paper investigates the potential of GNS as feed supplement for ruminants on pasture and its use in other industries. After extracting the seed, the groundnut shell, accounts for roughly 21-29 per cent of the total weight of the nut. Despite the high lignin content of the shell that necessitates adequate processing before use in animal feed, groundnut shell includes 0.50 per cent crude protein, 59.0 per cent crude fiber, 2.50 per cent ash and 4.43 per cent carbs. Sodium (42.00 mg/100 g), potassium (705.11 mg/100 g), magnesium (3.98.00 mg/100 g), calcium (2.28 mg/100 g), iron (6.97 mg/100 g), zinc (3.20 mg/100 g) and phosphorus (10.55 mg/100 g) are all abundant in groundnut shells. In view of this, GNS, a by-product of industrial processing of groundnuts is a rich source of nutrients and can be used to supplement ruminants on pastures during times when pastures are in short supply and of poor quality. Studies are needed to investigate their use to supplement cattle on pasture grazing during times of feed shortage. However, its use as animal feed supplement is likely to face challenges from other industries such as biofuel production.

Markets for Purchased Farm Inputs

2005 ◽  
pp. 60-71
Author(s):  
E. Serova ◽  
O. Shick
Keyword(s):  
Agricultural Policy ◽  
Financial Constraints ◽  
Information Sources ◽  
Animal Feed ◽  
Government Policies ◽  
Market Institutions ◽  
Policy Makers ◽  
Government Organizations ◽  
The Impact ◽  
Farm Inputs

Russian policy makers argue that agriculture suffers from decapitalization due to financial constraints faced by producers. This view is the basis for the national agricultural policy, which emphasizes reimbursement of input costs and substitutes government and quasi-government organizations for missing market institutions. The article evaluates the availability of purchased farm inputs, the efficiency of their use, the main problems in the emergence of market institutions, and the impact of government policies. The analysis focuses on five groups of purchased inputs: farm machinery, fertilizers, fuel, seeds, and animal feed. The information sources include official statistics and data from two original surveys.

ACOUSTIC PANEL CHICKEN FEATHER WASTE ENVIRONMENTALLY FRIENDLY

2020 ◽  
Author(s):  
Ansarullah ◽  
Ramli Rahim ◽  
Baharuddin Hamzah ◽  
Asniawaty Kusno ◽  
Muhammad Tayeb
Keyword(s):  
Animal Feed ◽  
Environmentally Friendly ◽  
Selling Price ◽  
Chicken Feather ◽  
Chicken Feathers ◽  
The Road ◽  
Feather Waste ◽  
Chicken Feather Waste ◽  
Almost All ◽  
The Impact

Chicken feathers are the result of waste from slaughterhouses and billions ofkilograms of waste produced by various kinds of poultry processing. This hal is a veryserious problem for the environment because it causes the impact of pollution. Hasmany utilization of chicken feather waste such as making komocen, accessories,upholstery materials, making brackets to the manufacture of animal feed but from theresults of this activity cannot reduce the production of chicken feathers that hiscontinuously increase every year. This is due to the fact that the selling price of chickenmeat has been reached by consumers with middle to upper economic levels. This caneasily be a chicken menu in almost all restaurants and restaurants to the food stalls onthe side of the road. An alternative way of utilizing chicken feathers is to makecomposite materials in the form of panels. Recent studies have shown that the pvacmaterial can be utilized as a mixing and adhesive material with mashed or groundfeathered composites to form a panel that can later be used as an acoustic material.The test results show that the absorption of chicken feathers and pvac glue into panelscan absorb sound well with an absorption coefficient of 0.59, light. This result is veryeconomical so it is worth to be recommended as an acoustic material. Apart from theresults of research methods carried out is one of the environmentally friendly activitiesin particular the handling of waste problems

The sugar and alcohol industry in the biofuels and cogeneration era: a paradigm change (part II)

2014 ◽  
pp. 97-104 ◽  
Author(s):  
Electo Eduardo Silv Lora ◽  
Mateus Henrique Rocha ◽  
José Carlos Escobar Palacio ◽  
Osvaldo José Venturini ◽  
Maria Luiza Grillo Renó ◽  
...  
Keyword(s):  
Large Scale ◽  
New Technologies ◽  
Process Integration ◽  
Animal Feed ◽  
Raw Materials ◽  
Biofuel Production ◽  
New Paradigm ◽  
Alcohol Industry ◽  
Feed Production ◽  
Steam Parameters

The aim of this paper is to discuss the major technological changes related to the implementation of large-scale cogeneration and biofuel production in the sugar and alcohol industry. The reduction of the process steam consumption, implementation of new alternatives in driving mills, the widespread practice of high steam parameters use in cogeneration facilities, the insertion of new technologies for biofuels production (hydrolysis and gasification), the energy conversion of sugarcane trash and vinasse, animal feed production, process integration and implementation of the biorefinery concept are considered. Another new paradigm consists in the wide spreading of sustainability studies of products and processes using the Life Cycle Assessment (LCA) and the implementation of sustainability indexes. Every approach to this issue has as an objective to increase the economic efficiency and the possibilities of the sugarcane as a main source of two basic raw materials: fibres and sugar. The paper briefly presents the concepts, indicators, state-of-the-art and perspectives of each of the referred issues.

AN ECONOMIC STUDY FOR THE IMPACT OF BIOFUEL PRODUCTION EXPANSION

2018 ◽  
Vol 3 (2) ◽  
pp. 259-260
Author(s):  
I. S. Ali ◽  
A. M. Taryal ◽  
S. A. Abou-El Naga ◽  
M. M. Abd-El Menem
Keyword(s):  
Biofuel Production ◽  
Economic Study ◽  
The Impact
Sign in / Sign up

Export Citation Format

Share Document